Review
Cell Biology
That Minh Pham, Julie Ladeby Erichsen, Justyna Magdalena Kowal, Soren Overgaard, Hagen Schmal
Summary: Patients with intra-articular fractures have significantly elevated pro-inflammatory cytokines in synovial fluid after injury, along with increased levels of anti-inflammatory cytokines. However, concentrations of IL-13, CTX-II, and aggrecan did not show significant differences between compared cohorts. Further standardized studies focusing on the ratio of pro- and anti-inflammatory cytokines at different time points are needed to better understand the inflammatory responses in intra-articular fractures.
Article
Engineering, Biomedical
Hao Yao, Jiankun Xu, Jiali Wang, Yifeng Zhang, Nianye Zheng, Jiang Yue, Jie Mi, Lizhen Zheng, Bingyang Dai, Wenhan Huang, Shuhang Yung, Peijie Hu, Yechun Ruan, Qingyun Xue, Kiwai Ho, Ling Qin
Summary: The combination therapy of magnesium ions and vitamin C significantly alleviated joint destruction and pain in osteoarthritis, with sustained effects for up to 3 months. Vitamin C enhanced the promotive effect of magnesium ions on HIF-1 alpha expression in cartilage, and the combination also promoted M2 polarization of macrophages in the synovium.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Cristian Guarise, Davide Ceradini, Martina Tessari, Mauro Pavan, Stefano Moro, Veronica Salmaso, Carlo Barbera, Riccardo Beninatto, Devis Galesso
Summary: A new class of MMP3Is based on a small peptide backbone derivatized with an alkyl chain was developed, showing effective inhibitory activity and water solubility for potential treatment of osteoarthritis. The most effective MMPI, e(I), reversed inflammatory outcomes in vitro and promoted stable micellar formulation at higher concentrations in water, potentially increasing bioavailability and efficacy of the MMPIs.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Guillermo Bauza-Mayol, Marcos Quintela, Ava Brozovich, Michael Hopson, Shazad Shaikh, Fernando Cabrera, Aaron Shi, Federica Banche Niclot, Francesca Paradiso, Emma Combellak, Tom Jovic, Paul Rees, Ennio Tasciotti, Lewis W. Francis, Patrick Mcculloch, Francesca Taraballi
Summary: The use of biomimetic scaffolds in cartilage tissue engineering shows promising results in enhancing cartilaginous tissue formation and suppressing host cartilage degeneration. This approach may provide a clinical solution for cartilage tissue repair by modulating the immune environment to favor regenerative processes and suppress cartilage degeneration.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Orthopedics
Nicholas B. Allen, Bijan Abar, Richard M. Danilkowicz, Virginia B. Kraus, Steven A. Olson, Samuel B. Adams
Summary: Intra-articular ankle fracture can lead to cartilage damage through the activation of inflammatory cytokines and MMPs in synovial fluid fracture hematoma. However, the addition of IL-1Ra or doxycycline can partially attenuate this damaging effect on cartilage without affecting chondrocyte survival.
FOOT & ANKLE INTERNATIONAL
(2022)
Article
Food Science & Technology
Chengcheng Ma, Mingxiao Yu, Zhoujin Huang, Jingfeng Wang, Xue Zhao, Chunmei Kang, Hao Xu, Yanchao Wang, Hu Hou
Summary: The study demonstrates that administration of chicken sternal cartilage hydrolysates (CCH) can effectively prevent osteoarthritis by inhibiting abnormal subchondral bone formation, preventing osteophyte formation, and ameliorating cartilage damage through improving collagen metabolism.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Review
Chemistry, Medicinal
Oliver McClurg, Ryan Tinson, Linda Troeberg
Summary: Osteoarthritis is a common, degenerative joint disease with no disease-modifying drugs available for treatment. Current research focuses on inhibiting metalloproteinase-mediated cartilage degradation and promoting growth factor-driven repair to counteract cartilage damage. Approaches such as metalloproteinase-blocking antibodies, FGF18, and Wnt pathway inhibitors are being explored in clinical trials for potential therapeutic development.
Review
Immunology
James Orman Early, Lauren E. Fagan, Annie M. Curtis, Oran D. Kennedy
Summary: Inflammation is an important biological response to tissue damage, but dysregulation of the process can lead to tissue damage. Mitochondria play a key role in orchestrating the inflammatory response, beyond their traditional function in energy production. Osteoarthritis, previously thought of as non-inflammatory, now appears to involve chronic inflammation as well.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
J. Alberto Gines
Summary: This study provides initial information on the effect of platelet rich plasma on the most important pro-inflammatory cytokines in dogs with osteoarthritic joint. The results suggest that LrPRP can modulate the ratios of pro-inflammatory cytokine TNF-alpha in dogs with CCLR.
Article
Rheumatology
James MacKay, Ali Guermazi, C. Kent Kwoh, P. L. Paul See, Mohamed Jarraya, Ling Li, Michael J. Hannon, Tomoko Fuji, Frank W. Roemer
Summary: This study aims to describe the frequencies of osteophytes and co-localized cartilage damage as well as evaluate their associations in knees with incident tibiofemoral radiographic knee osteoarthritis (OA). The results showed that the patellofemoral joint had more severe cartilage damage compared to other compartments, while cartilage damage in the posterior femur was rare.
ARTHRITIS CARE & RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ke Li, Xing Ji, Rebecca Seeley, Wen-Chih Lee, Yu Shi, Fangfang Song, Xueyang Liao, Chao Song, Xiaobin Huang, Danielle Rux, Ju Cao, Xiaoji Luo, Steven M. Anderson, Wei Huang, Fanxin Long
Summary: This study found that the expression of glucose transporter Glut1 is reduced in articular cartilage in osteoarthritis (OA). Inducible deletion or forced expression of Glut1 can accelerate or protect against cartilage destruction, respectively. Moreover, in diabetic mice, both Glut1 expression and glycolysis rate are diminished in articular cartilage, resulting in more severe cartilage destruction.
Article
Biotechnology & Applied Microbiology
Manon Jammes, Romain Contentin, Fabrice Audigie, Frederic Casse, Philippe Galera
Summary: This study tested the effects of different storage conditions on the secretome of mesenchymal stromal cells (MSCs) and investigated the enhancement of MSC secretome properties through cytokine priming. The results showed that secretome stored at -80°C, -20°C, and 4°C improved collagen protein accumulation, cell proliferation, and inflammation reduction in chondrocytes. Cytokine priming had different effects on the expression of immunomodulatory molecules and influenced the behavior of equine articular chondrocytes (eACs). These findings demonstrate the potential of MSC-conditioned media for improving the management of equine osteoarthritis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Phaedra Winstanley-Zarach, Gregor Rot, Shweta Kuba, Aibek Smagul, Mandy J. J. Peffers, Simon R. R. Tew
Summary: Polyadenylation (polyA) defines the 3' boundary of a transcript's genetic information. Alternative polyadenylation (APA) transcripts can exist for a gene, causing variance in regulatory domains and potential coding sequence changes. This study analyzed the polyA site distribution in healthy and osteoarthritic knee articular chondrocytes and found modest differential use of polyadenylation sites between the two conditions, suggesting potential functional relevance in affected genes. This characterization of polyadenylation landscape contributes to understanding the role of polyA in knee osteoarthritis, distinguishing it from other pathologies such as cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Joachim C. Manning, Veronica Baldoneschi, Laura L. Romero-Hern, Katharina M. Pichler, Gabriel GarcIa Caballero, Sabine Andr, Tanja J. Kutzner, Anna-Kristin Ludwig, Valerio Zullo, Barbara Richichi, Reinhard Windhager, Herbert Kaltner, Stefan Toegel, Hans-Joachim Gabius, Paul V. Murphy, Cristina Nativi
Summary: Pairing glycans with tissue lectins can regulate multiple effector pathways in (patho)physiology, and it is particularly important in the progression of osteoarthritis. Designing inhibitors that can block galectin binding and matrix metalloproteinases (MMPs) has the potential to dissect the roles of lectins and proteolytic enzymes. Testing these inhibitors on clinical material has shown promising results in reducing galectin binding to osteoarthritis specimens.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)