Journal
FEBS LETTERS
Volume 589, Issue 24, Pages 4088-4096Publisher
WILEY
DOI: 10.1016/j.febslet.2015.11.021
Keywords
Extracellular HSP70; Human mesenchymal stem cell; Osteogenesis; ERK signaling pathway; Bone nonunion
Funding
- National Natural Science Foundation of China [81271973, 81201397]
- Zhejiang Provincial Natural Science Foundation of China [LY15H060001, LY15H060002, LY13H060002]
- Zhejiang medical and health science and technology plan project [2015116473]
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Heat shock proteins have protective effects when cells are exposed to stress. However, the relationship between extracellular heat shock protein 70 (eHSP70) and osteogenesis of hMSCs has not been reported. The results of this study showed that HSP70 (200 ng/ml) increases alkaline phosphatase activity and promotes hMSC mineralization. Under osteogenic induction conditions, HSP70 significantly upregulated the expression of osteo-specific genes, such as the runt family transcription factor Runx2 and osterix (OSX). Comparative expression profiling by microarray and pathway analyses revealed that HSP70 promotes osteogenesis of hMSCs through activation of the ERK signaling pathway. HSP70 may be a potential therapeutic agent for the treatment of bone nonunion. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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