Journal
FEBS JOURNAL
Volume 282, Issue 6, Pages 1043-1064Publisher
WILEY
DOI: 10.1111/febs.13201
Keywords
Canavaliaensiformis; circular dichroism; Jaburetox; light scattering; NMR spectroscopy; urease
Categories
Funding
- Brazilian agency Conselho de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [PVE 054/2012]
- Ciencias sem Fronteiras (Science without Borders)
- CAPES a split PhD fellowship at the University of Bologna
- Specialty Fertilizer Products (Leawood, KS, USA)
- CIRMMP (Consorzio Interuniversitario di Risonanze Magnetiche di Metallo-Proteine)
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Belgium [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of France [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Italy [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Germany [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of the Netherlands
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Poland [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Portugal [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Spain [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of UK [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of South Africa [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Malaysia [261572]
- WeNMR project (European FP7 e-Infrastructure grant) by European Grid Initiative through the national GRID Initiative of Taiwan [261572]
- Latin America GRID infrastructure via the Gisela project
- International Desktop Grid Federation
Ask authors/readers for more resources
Jaburetox is a polypeptide derived from jack bean (Canavaliaensiformis) urease and toxic to a broad spectrum of insects, phytopathogenic filamentous fungi and yeasts of medical importance. The elucidation of the structural basis for the mode of action of Jaburetox is the focus of this multifaceted study. Jaburetox in solution is a monomer of 11.0kDa featuring a large hydrodynamic radius, suggestive of a disordered polypeptide. The intrinsically disordered nature of Jaburetox was theoretically predicted by a comprehensive bioinformatics analysis and experimentally confirmed by light scattering as well as by circular dichroism and NMR spectroscopy. NMR signal assignment provided backbone secondary chemical shifts that indicated that Jaburetox has a low propensity to assume a stable secondary structure. N-15 relaxation studies revealed significant backbone mobility, especially in the N-terminal portion of the polypeptide. The solution structure of Jaburetox shows the presence of an -helical motif close to the N terminus, together with two turn-like structures situated in the central portion of the protein and close to the C terminus. Similar regions were predicted as potential protein-protein interaction sites using computational tools. The knowledge of the structural properties of Jaburetox in solution is a key step to correlate its structural and biological activities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available