Article
Peripheral Vascular Disease
Luke. S. Dunaway, Marie Billaud, Edgar Macal, Miranda. E. Good, Christopher. B. Medina, Ulrike Lorenz, Kodi Ravichandran, Michael Koval, Brant. E. Isakson
Summary: This study suggests that Panx1 in smooth muscle cells plays a role in regulating sympathetic nerve constriction and blood pressure. The findings also demonstrate the feasibility of using Panx1-mouse models to test potential drugs for hypertension.
Article
Cardiac & Cardiovascular Systems
Lei Wang, Megan Rice, Sandra Swist, Thomas Kubin, Fan Wu, Shengpeng Wang, Simone Kraut, Norbert Weissmann, Thomas Boettger, Matthew Wheeler, Andre Schneider, Thomas Braun
Summary: The study revealed that BMP9 and BMP10 act directly on VSMCs for induction and maintenance of their contractile state. The effects of BMP9/10 in VSMCs are mediated by different combinations of BMP type 1 receptors in a vessel bed-specific manner, offering new opportunities to manipulate blood pressure in the pulmonary circulation.
Article
Cardiac & Cardiovascular Systems
Benjamin Grimmer, Adrienn Krauszman, Xudong Hu, Golam Kabir, Kim A. Connelly, Mei Li, Jana Grune, Christian Madry, Brant E. Isakson, Wolfgang M. Kuebler
Summary: This study identifies Panx1 as a novel regulator of HPV, but its role is not necessarily linked to ATP release and downstream signaling via P2Y receptors or TRPV4 activation. Instead, Panx1 appears to modulate the Ca2+ response of PASMC to hypoxia. Furthermore, Panx1 does not affect the development of chronic hypoxic PH.
CARDIOVASCULAR RESEARCH
(2022)
Article
Sport Sciences
Sophie Moller, Camilla Collin Hansen, Thomas Svare Ehlers, Andrea Tamariz-Ellemann, Sarah A. Rogvi Tolborg, Melanie Emmonds Kurell, Jorge Perez-Gomez, Simon Schultz Patrzalek, Christine Maulitz, Ylva Hellsten, Lasse Gliemann
Summary: This study aimed to determine the mechanisms behind the reduction of blood pressure through exercise training. The results showed that the exercise training-induced lowering of arterial blood pressure in nonmedicated hypertensive men does not involve changes in the function of pannexin 1. Training, however, can enhance leg vascular conductance and improve the vasodilator response to acetylcholine.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Article
Peripheral Vascular Disease
Christine A. Klemens, Evgeny G. Chulkov, Jing Wu, Md Abdul Hye Khan, Vladislav Levchenko, Michael J. Flister, John D. Imig, Alison J. Kriegel, Oleg Palygin, Alexander Staruschenko
Summary: Studies have shown that ClC-6 may modulate blood pressure by regulating Golgi calcium reserves, thereby affecting vascular smooth muscle function.
Article
Biology
Zdravka Daneva, Matteo Ottolini, Yen Lin Chen, Eliska Klimentova, Maniselvan Kuppusamy, Soham A. Shah, Richard D. Minshall, Cheikh Seye, Victor E. Laubach, Brant E. Isakson, Swapnil K. Sonkusare
Summary: The study showed that endothelial Panx1 signaling pathway contributes to vasodilation and reduces pulmonary arterial pressure through ATP-TRPV4 channel signaling. By regulating ATP efflux and TRPV4 channel activity, Panx1 could potentially be targeted for the treatment of pulmonary arterial hypertension and related diseases.
Article
Cardiac & Cardiovascular Systems
Iain R. Lamb, Nicole M. Novielli-Kuntz, Coral L. Murrant
Summary: This study identified a pannexin/purinergic-dependent communication pathway between capillaries and upstream arterioles, indicating a new mechanism for blood flow control in skeletal muscle. The involvement of both pannexin/purinergic-dependent and gap-junction-dependent pathways in coordinating skeletal muscle blood flow was demonstrated.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Derek M. Dykxhoorn, Huilan Wang, Andrea Da Fonseca Ferreira, Jianqin Wei, Chunming Dong
Summary: Cocaine abuse increases the risk of cardiovascular diseases and hypertension. This study focused on the role of miRNAs in mediating the cardiovascular effects of cocaine. Multiple miRNAs showed altered expression after cocaine exposure in the cardiovascular system. Specifically, miR-423-5p and its target gene Cacna2d2 were found to regulate intracellular calcium concentration and smooth muscle cell contractility. Manipulation of the miR-423-5p-Cacna2d2-calcium transport pathway may provide a novel therapeutic strategy for cocaine-induced hypertension and aortic stiffness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ksenia A. Zaripova, Ekaterina P. Kalashnikova, Svetlana P. Belova, Tatiana Y. Kostrominova, Boris S. Shenkman, Tatiana L. Nemirovskaya
Summary: The findings suggest that PANX1 ATP-permeable channels play a crucial role in regulating muscle atrophy processes by modulating the expression of E3 ligases, as well as protein translation and elongation processes during unloading.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Peripheral Vascular Disease
Stephanie M. Mutchler, Annet Kirabo, Thomas R. Kleyman
Summary: The development of high blood pressure is influenced by genetic and environmental factors, with high salt intake being a known environmental contributor. There is a spectrum of sodium-sensitivity among individuals, partly due to genetic variation in pathways involved in sodium handling and excretion. ENaC plays a crucial role in regulating extracellular fluid volume and blood pressure by facilitating sodium reabsorption in the distal nephron.
Article
Cardiac & Cardiovascular Systems
Yen-Lin Chen, Zdravka Daneva, Maniselvan Kuppusamy, Matteo Ottolini, Thomas M. Baker, Eliska Klimentova, Soham A. Shah, Jennifer D. Sokolowski, Min S. Park, Swapnil K. Sonkusare
Summary: This study demonstrates the role of TRPV4(SMC) channels in blood pressure regulation, showing that their activity increases resting blood pressure in normal mice. The study also reveals the opposite effects of α1AR-TRPV4(SMC) signaling and intraluminal pressure-TRPV4(SMC)-BK channel signaling on blood pressure. In hypertension, constrictor α1AR-PKCα-TRPV4 signaling is upregulated, while dilator pressure-TRPV4-BK channel signaling is disrupted, leading to increased vasoconstriction and elevated blood pressure.
Article
Cardiac & Cardiovascular Systems
Melissa A. Luse, Nenja Kruger, Miranda E. Good, Lauren A. Biwer, Vlad Serbulea, Anita Salamon, Rebecca A. Deaton, Norbert Leitinger, Axel Godecke, Brant E. Isakson
Summary: This study reveals the important role of the fat mass obesity (FTO) gene in regulating smooth muscle contractility, possibly through methylation of serum response factor (Srf).
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ola El Atab, Crystal M. Ghantous, Nabil El-Zein, Rima Farhat, Abdelali Agouni, Hesham M. Korashy, Laiche Djouhri, Layla Kamareddine, Kazem Zibara, Asad Zeidan
Summary: This study investigates the molecular mechanisms involved in hyperglycemia-induced vascular remodeling, with a focus on the role of APN and leptin. It found that hyperglycemia increases APN synthesis but decreases APN receptor expression, and upregulates leptin expression in vascular smooth muscle cells. These changes lead to increased oxidative stress and alterations in actin cytoskeleton dynamics, facilitating vascular remodeling.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Jeff Arni C. Valisno, Joel May, Kuldeep Singh, Eric Y. Helm, Lisia Venegas, Enkhjargal Budbazar, Jena B. Goodman, Christopher J. Nicholson, Dorina Avram, Richard A. Cohen, Gary F. Mitchell, Kathleen G. Morgan, Francesca Seta
Summary: The study reveals that BCL11B is expressed in vascular smooth muscle and regulates vascular stiffness. RNA sequencing in Bcl11b null mice identified changes in signaling pathways leading to increased arterial stiffness and cardiovascular damage.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Brett S. Kirby, Matthew A. Sparks, Eduardo R. Lazarowski, Denise A. Lopez Domowicz, Hongmei Zhu, Timothy J. McMahon
Summary: The study found that Panx1 channels participate in hypoxic vasodilation by regulating hypoxia-sensitive extracellular ATP levels in blood. Mice lacking Panx1 showed attenuated decrease in blood pressure and reduced increase in limb blood flow during hypoxia, along with lower plasma ATP levels. This indicates the important role of Panx1 in vascular responses to hypoxia.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Physiology
T. C. Stevenson Keller, Christophe Lechauve, Alexander S. Keller, Steven Brooks, Mitchell J. Weiss, Linda Columbus, Hans Ackerman, Miriam M. Cortese-Krott, Brant E. Isakson
Summary: Globin proteins exist in various cell types of the vasculature and have the ability to interact with molecular oxygen and nitric oxide. They play important roles in regulating vascular contraction, tissue perfusion, and vascular reactivity. Understanding the impact of globin proteins on vascular physiology is of great significance for further research on their functions.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Oncology
Yoshihiro Otani, Ji Young Yoo, Cole T. Lewis, Samantha Chao, Jessica Swanner, Toshihiko Shimizu, Jin Muk Kang, Sara A. Murphy, Kimberly Rivera-Caraballo, Bangxing Hong, Joseph C. Glorioso, Hiroshi Nakashima, Sean E. Lawler, Yeshavanth Banasavadi-Siddegowda, John D. Heiss, Yuanqing Yan, Guangsheng Pei, Michael A. Caligiuri, Zhongming Zhao, E. Antonio Chiocca, Jianhua Yu, Balveen Kaur
Summary: This study evaluated the impact of NOTCH signaling on virus-induced immunotherapy. The results showed that NOTCH signaling was significantly correlated with myeloid cell infiltration, and NOTCH-activated macrophages promoted the secretion of CCL2 in the tumor microenvironment, leading to the expansion of myeloid-derived suppressor cells. Pharmacologic blockade of NOTCH signaling improved the immunosuppressive tumor microenvironment and activated a CD8-dependent antitumor memory response, resulting in therapeutic benefits.
CLINICAL CANCER RESEARCH
(2022)
Article
Endocrinology & Metabolism
C. Brent Wakefield, Vanessa R. Lee, Danielle Johnston, Parastoo Boroumand, Nicolas J. Pillon, Samar Sayedyahossein, Brooke L. O'Donnell, Justin Tang, Rafael E. Sanchez-Pupo, Kevin J. Barr, Robert Gros, Lauren Flynn, Nica M. Borradaile, Amira Klip, Frank Beier, Silvia Penuela
Summary: PANX3 expression in male adult mouse adipose tissue may regulate adipocyte numbers, influencing fat accumulation and inflammation. Male KO mice fed a chow diet had lower body weight and lower fat mass, reduced to the same extent as WT mice with forced exercise, suggesting a potential role of PANX3 in regulating body weight. Additionally, KO mice exhibited a more anti-inflammatory phenotype on a high-fat diet compared to WT mice, with significantly lower expression of multiple pro-inflammatory genes in white adipose tissue.
INTERNATIONAL JOURNAL OF OBESITY
(2022)
Article
Hematology
Yiqing Yang, Melanie Mumau, Joanna Tober, Qin Zhu, Laura Bennett, Courtney Hong, Derek Sung, Thomas Keller, Yasin Uzun, Peng Gao, Swapnil Shewale, Mei Chen, Jisheng Yang, Xiaowen Chen, Steven A. Thomas, Kai Tan, Nancy A. Speck, Mark L. Kahn
Summary: This study identifies the MEKK3-KLF2/4 signaling pathway as essential for the specification of hemogenic endothelial cells and their transition to intraaortic hematopoietic cluster cells during embryonic hematopoiesis. Inflammatory mediators and fluid shear forces can activate this signaling pathway and promote the process.
Meeting Abstract
Biochemistry & Molecular Biology
Abigail G. Wolpe, Melissa A. Luse, Scott R. Johnstone, Jianxiang Xue, Vikram Sabapathy, Brent Wakefield, Rahul Sharma, Kevin Barr, Frank Beier, Dale Laird, Stefanie Redemann, Linda Columbus, Silvia Penuela, Brant E. Isakson
Article
Cardiac & Cardiovascular Systems
Leon J. DeLalio, Sean D. Stocker
Summary: The circadian cycle affects sympathetic and hemodynamic responses to activation of renal chemosensitive sensory fibers. Responses to intrarenal bradykinin or capsaicin infusion were exaggerated during nighttime, but responses to elevated renal pelvic pressure were not. Importantly, renal afferent nerve responses were not different between nighttime and daytime.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Peripheral Vascular Disease
Luke. S. Dunaway, Marie Billaud, Edgar Macal, Miranda. E. Good, Christopher. B. Medina, Ulrike Lorenz, Kodi Ravichandran, Michael Koval, Brant. E. Isakson
Summary: This study suggests that Panx1 in smooth muscle cells plays a role in regulating sympathetic nerve constriction and blood pressure. The findings also demonstrate the feasibility of using Panx1-mouse models to test potential drugs for hypertension.
Review
Physiology
Brent Wakefield, Silvia Penuela
Summary: Pannexins, expressed in the cardiovascular and musculoskeletal system, play important roles in cell signaling through the release of nucleotides and calcium channel activation. Exercise training has been shown to influence pannexin expression and may have implications in certain diseases.
JOURNAL OF VASCULAR RESEARCH
(2023)
Review
Physiology
Meghan W. Sedovy, Xinyan Leng, Melissa R. Leaf, Farwah Iqbal, Laura Beth Payne, John C. Chappell, Scott R. Johnstone
Summary: Connexin 43 (Cx43) plays a vital role in vascular function by enabling communication between vascular cells and influencing cell signaling and vasomotor activity. It is expressed in various vascular cell types and its expression and function differ depending on vessel size and location. Alterations in Cx43 expression, localization, and post-translational modification have been observed in vascular diseases such as atherosclerosis, hypertension, and diabetes.
JOURNAL OF VASCULAR RESEARCH
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Xiaobo Wu, David King, Gregory Hoeker, Scott R. Johnstone, Robert G. Gourdie, Seth Weinberg, Steven Poelzing
CIRCULATION RESEARCH
(2022)
Meeting Abstract
Hematology
Louisa Mezache, Andrew Soltisz, Scott R. Johnstone, Brant Isakson, Rengasayee Veeraraghavan
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Proceedings Paper
Acoustics
Mahsa Bataghva, Student Member, Danielle Johnston, Nicholas Power, Aaron Ward, Silvia Penuela, James C. Lacefield
Summary: This study assesses the usefulness of perfusion parameters from different ultrasound images in classifying the anti-angiogenic tumor treatment response. The results indicate that the model with selected parameters from SVD-filtered PD images outperforms other models and can be used as a supplement to conventional PD and CEUS analysis.
2022 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IEEE IUS)
(2022)
Meeting Abstract
Dermatology
Harry Scott, Li Dong, Sharmila Srinivasan, Andrew Stevenson, Alasdair MacDonald, Paola Massimi, Lawrence Banks, Malcolm Hodgins, Patricia Martin, Scott Johnstone, Sheila Graham
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Meeting Abstract
Biophysics
Louisa Mezache, Andrew M. Soltisz, Scott R. Johnstone, Brant Isakson, Rengasayee Veeraraghavan
BIOPHYSICAL JOURNAL
(2022)