Article
Dentistry, Oral Surgery & Medicine
H. Zheng, J. Fu, Z. Chen, G. Yang, G. Yuan
Summary: This study found that Mdm2 plays a crucial role in dentin formation by interacting and ubiquitinating Dlx3, which regulates odontoblast differentiation and transcriptional activity of Dspp gene.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Youjing Qiu, Takashi Saito
Summary: The novel bioactive adhesive monomer CMET demonstrated low cytotoxicity and high odontogenic differentiation capacity on human dental pulp stem cells. In a rat pulp injury model, CMET induced continuous hard tissue bridge formation at the pulp exposure site, with a regular dentinal tubule pattern. These findings suggest that CMET may serve as a promising adjunctive for pulp-capping materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
H. Zheng, Y. Shi, L. Bi, Z. Zhang, Z. Zhou, C. Shao, D. Cui, X. Cheng, R. Tang, H. Pan, Z. Wu, B. Fu
Summary: Methacryloyloxydecyl dihydrogen phosphate (MDP) has the ability to induce mineralization. Through various techniques, we demonstrate that MDP can demineralize dentin and infiltrate collagen fibrils to induce intrafibrillar mineralization. This dual functionality of MDP could have significant implications in adhesive dentistry and preventive dentistry.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Elio Minetti, Andrea Palermo, Giuseppina Malcangi, Alessio Danilo Inchingolo, Antonio Mancini, Gianna Dipalma, Francesco Inchingolo, Assunta Patano, Angelo Michele Inchingolo
Summary: This study analyzed the biochemical characteristics of dentin granules, demineralized dentin granules, and cortical bone granules. The results showed that demineralized dentin is similar to natural bone in terms of their chemical composition, which makes it a potential alternative to autogenous bone in regenerative surgery.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Dentistry, Oral Surgery & Medicine
Anita Aminoshariae, James C. Kulild
Summary: The study identified five different mechanisms of dentinal sensitivity and suggested that these mechanisms may interact. Pulpal responses to tissue injury may alter the relative contribution of these mechanisms, for example, pulpal inflammation may lead to neuronal sprouting and peripheral sensitization.
JOURNAL OF ENDODONTICS
(2021)
Article
Biotechnology & Applied Microbiology
Anna Zawadzka-Knefel, Agnieszka Rusak, Monika Mrozowska, Tomasz Machalowski, Andrzej Zak, Katarzyna Haczkiewicz-Lesniak, Michal Kulus, Piotr Kuropka, Marzenna Podhorska-Okolow, Katarzyna Skoskiewicz-Malinowska
Summary: The use of stem cells for tissue regeneration is a prominent trend in regenerative medicine and tissue engineering. Dental pulp stem cells (DPSCs) have attracted attention due to their ability to differentiate into osteoblasts and odontoblasts. This study explored the biological responses of human DPSCs to a purified 3D chitin scaffold derived from the marine demosponge Aplysina fistularis. Results showed that the chitin scaffold, especially with hydroxyapatite (HAp) modification, supported cell adhesion, proliferation, and differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Physiology
Huihui Ren, Quan Wen, Qingxuan Zhao, Nan Wang, Yuming Zhao
Summary: Dental pulp plays a crucial role in maintaining tooth function and pulp-regeneration therapy shows promise in clinical practice. However, the mechanisms of pulp regeneration and the role of dental stem cells are not fully understood. This study used single-cell RNA sequencing analysis to explore the development of dental pulp and stem cells at different stages. The results revealed temporal changes in the composition of pulp cells and identified subpopulations of odontoblasts and mesenchymal stem cells. These findings may provide insights into tooth development and improve the success of dental pulp regeneration.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Cell Biology
Mengmeng Liu, Graham Goldman, Mary MacDougall, Shuo Chen
Summary: BMP signaling plays a crucial role in dentin development, regulating the differentiation and gene expression of odontoblasts through canonical and non-canonical Smad signaling pathways. Dysregulation of BMP signaling leads to tooth disorders, and studies in mice with BMP signaling-associated gene mutations or knockouts have provided insights into the underlying networks. This review summarizes recent advances in understanding BMP signaling in odontoblast differentiation and dentin formation, including the expression of BMPs, receptors, and downstream genes, as well as the structures of BMPs, receptors, antagonists, and their association with dentin defects.
Article
Chemistry, Multidisciplinary
Elio Minetti, Andrea Grassi, Tomas Beca Campoy, Andrea Palermo, Filiberto Mastrangelo
Summary: This study evaluated a novel flapless alveolar socket preservation (ASP) procedure. The results showed that using demineralized tooth dentin graft material covered by three reabsorbable membranes effectively promoted bone regeneration and achieved good clinical outcomes as well as maintenance of tissue architecture.
APPLIED SCIENCES-BASEL
(2023)
Article
Pharmacology & Pharmacy
E. Minetti, A. Palermo, A. D. Inchingolo, A. Patano, F. Viapiano, A. M. Ciocia, E. de Ruvo, A. Mancini, F. Inchingolo, S. Sauro, G. Malcangi, G. Dipalma, A. M. Inchingolo
Summary: This study examined the size of granules obtained by the Tooth Transformer (TT) device using a laser instrument. The results showed that 85% of the granules were in accordance with the dimensions suggested in the literature.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Anatomy & Morphology
Tsuyoshi Kano, Tsuyoshi Morita, Kaori Sumida, Hiromichi Yumoto, Otto Baba
Summary: The study suggests that FGF-FGFR signaling may play a crucial role in dentin formation, especially in secondary and tertiary dentin formation after tooth eruption. Analysis on mouse teeth revealed that Dspp and Fgfr2c had the highest expression levels at 2 weeks old.
ANATOMICAL SCIENCE INTERNATIONAL
(2021)
Article
Biology
Jila Nasirzade, Karol Ali Apaza Alccayhuaman, Zahra Kargarpour, Ulrike Kuchler, Franz Josef Strauss, Layla Panahipour, Carina Kampleitner, Patrick Heimel, Frank Schwarz, Reinhard Gruber
Summary: The study found that dentin lysate coating did not significantly affect bone regeneration in rat calvarial defects, with nearly complete defect closure even in untreated defects. Therefore, conclusions cannot be drawn regarding the stimulation of bone regeneration by dentin lysates.
Article
Chemistry, Physical
Md Arafat Kabir, Masaru Murata, Mamata Shakya, Katsuhisa Yamada, Toshiyuki Akazawa
Summary: The study evaluated the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, PR-DDM, in critical-size iliac defects after in vivo implantation. The morphological changes due to bio-absorption by the cellular phagocytes were comparable to the 3D micro-CT and histological images at 2 and 4 months, demonstrating that the PR-DDM block was gradually absorbed by multinucleated giant cells and regenerated bone. PR-DDM may serve as a unique scaffold for extraoral bone regeneration.
Article
Physiology
Sadao Ohyama, Takehito Ouchi, Maki Kimura, Ryuya Kurashima, Keiko Yasumatsu, Daisuke Nishida, Suzuro Hitomi, Sobhan Ubaidus, Hidetaka Kuroda, Shinichirou Ito, Masayuki Takano, Kentaro Ono, Toshihide Mizoguchi, Akira Katakura, Yoshiyuki Shibukawa
Summary: Recent evidence suggests that dentinal pain or sensitivity is caused by dentinal fluid movement, which activates mechanosensitive TRP/Piezo channels in odontoblasts. The communication between odontoblasts and neurons mediated by the Piezo1/TRPA1-pannexin-1-P2X(3) receptor axis is crucial for the development of dentinal pain.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Boon Chin Heng, Xuehui Zhang, Dominique Aubel, Yunyang Bai, Xiaochan Li, Yan Wei, Martin Fussenegger, Xuliang Deng
Summary: YAP and TAZ are crucial proteins involved in maintaining tissue/organ size and have diverse roles in various biological processes, including cell proliferation, tissue regeneration, cell fate determination, tumorigenesis, and mechanosensing.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yuanhao Xie, Chenqi Tang, Zizhan Huang, Sicheng Zhou, Yuwei Yang, Zi Yin, Boon Chin Heng, Weishan Chen, Xiao Chen, Weiliang Shen
Summary: In vitro extracellular matrix (ECM) remodeling is critical for maintaining stem cell (SC) function. This review summarizes how cells remodel ECM and the significance of ECM remodeling in ex vivo SC culture, as well as outlines the shortcomings of current research and future directions.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Engineering, Biomedical
Li Wang, Xiaolei Guo, Jiaqing Chen, Zhen Zhen, Bin Cao, Wenqian Wan, Yuandong Dou, Haobo Pan, Feng Xu, Zepu Zhang, Jianmei Wang, Daisong Li, Quanyi Guo, Qing Jiang, Yanan Du, Jiakuo Yu, Boon Chin Heng, Qianqian Han, Zigang Ge
Summary: With the interdisciplinary convergence of biology, medicine, and materials science, there is a growing need to bridge the gap between applied basic research and translational products in the field of biomaterials. This article critically examines the developmental pipeline of biodegradable biomaterials and proposes ideas to integrate different mindsets to guide research and translation, reducing unnecessary research efforts and funding.
BIOACTIVE MATERIALS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Mei Zhuang, Wuwen Zhang, Nuo Cheng, Ling Zhou, Dan Liu, Hua Yan, Ge Fang, Boon Chin Heng, Yan Sun, Guoqing Tong
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Engineering, Biomedical
Ting Zou, Shan Jiang, Baicheng Yi, Qixin Chen, Boon Chin Heng, Chengfei Zhang
Summary: The study demonstrated that SCAPs induced in BDNF-GelMA with small molecule cocktail showed significant upregulation of neural markers, exhibiting morphological characteristics similar to neuronal cells, which may provide a therapeutic platform for neurological disorders.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Cell Biology
Jiabei Yang, Xuewei Zhang, Jiaqing Chen, Boon Chin Heng, Yangzi Jiang, Xiaoyu Hu, Zigang Ge
Summary: The study highlights the important role of macrophages in cartilage regeneration, showing that they promote regeneration by modulating proliferation and apoptosis. Exogeneous macrophages have therapeutic potential in promoting cartilage regeneration by regulating stem cells. Only M2c macrophages are able to promote regeneration of both cartilage tissues and subchondral bone.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biology
Kejia Li, Litong Fan, Jianjing Lin, Boon Chin Heng, Zhantao Deng, Qiujian Zheng, Jue Zhang, Yangzi Jiang, Zigang Ge
Summary: Mesenchymal stem cells (MSCs) can benefit from enhanced chondrogenesis through the combination of nanosecond pulsed electric fields and ghrelin. The sequential order of applying these two stimuli is crucial for promoting cartilage regeneration. This study provides new insights and strategies for future cell-based therapies.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Materials Science, Biomaterials
Xiaohan Dai, Yunyang Bai, Boon Chin Heng, Yiping Li, Zhangui Tang, Changjian Lin, Ousheng Liu, Ying He, Xuehui Zhang, Xuliang Deng
Summary: This study designed biomimetic micro/sub-micro hierarchical surfaces on titanium implants to mitigate inflammatory reactions and osteoclastogenesis, enhance osteogenic differentiation, and promote early osseointegration. The biomimetic hierarchical surfaces inhibited M1 macrophage-mediated inflammatory reactions and decreased osteoclast formation. In vivo, the implants with hierarchical surfaces underwent rapid and early osseointegration. This study offers a novel biomimetic strategy for implant-bone osseointegration in osteoporotic patients.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Cell Biology
Peiwen He, Dengfeng Ruan, Zizhan Huang, Canlong Wang, Yiwen Xu, Honglu Cai, Hengzhi Liu, Yang Fei, Boon Chin Heng, Weishan Chen, Weiliang Shen
Summary: This review discusses the processes of tendon development and repair, involving various regulatory factors and signaling pathways. By studying and comparing these processes, it can provide guidance for the treatment of tendon injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Yaru Guo, Feng Mei, Ying Huang, Siqin Ma, Yan Wei, Xuehui Zhang, Mingming Xu, Ying He, Boon Chin Heng, Lili Chen, Xuliang Deng
Summary: This study demonstrates that matrix stiffness promotes sprouting of endothelial cells and regulates the formation of tip cells through a specific signaling axis. The findings have significant implications for biomaterial design and treatment of pathological conditions.
BIOACTIVE MATERIALS
(2022)
Review
Endocrinology & Metabolism
Jaydeep Roy, Kan Yin Wong, Luca Aquili, Md. Sahab Uddin, Boon Chin Heng, George Lim Tipoe, Kah Hui Wong, Man Lung Fung, Lee Wei Lim
Summary: In this review, the developmental trends and neuroprotective mechanisms of melatonin therapies for Alzheimer's disease (AD) are examined. Melatonin has been shown to alleviate various pathological processes in AD and beneficially affect behavior in animal models. Clinical studies demonstrate positive effects of melatonin on sleep, cognition, behavior, and psychiatric symptoms in AD patients.
FRONTIERS IN NEUROENDOCRINOLOGY
(2022)
Article
Chemistry, Physical
Ruyuan Ding, Yajun Liu, Dawei Cheng, Gang Yang, Wenjing Wu, Haoran Du, Xin Jin, Yihan Chen, Yuanyin Wang, Boon Chin Heng, Qing Yang, Jianguang Xu
Summary: The incorporation of pro-osteogenic growth factors into bone graft materials to enhance bone regeneration is a key research area. The combination of gene therapy and tissue engineering through gene-activated matrix (GAM) provides a solution to overcome the limitations of growth factors degradation. In this study, a novel GAM system with superior bone regeneration capacity was prepared, and in vitro experiments demonstrated excellent biocompatibility and sustained release of therapeutic genes.
Review
Dentistry, Oral Surgery & Medicine
Boon Chin Heng, Yunyang Bai, Xiaochan Li, Xuehui Zhang, Xuliang Deng
Summary: Dental and oral-derived stem cells have high potential for neurogenesis. Using electroactive or electroconductive tissue engineering scaffolds may enhance their neurogenesis. However, there is limited research on these cells, with most scientific information coming from studies on other mesenchymal stem cell lineages.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Engineering, Biomedical
Fangyuan Bao, Junzhi Yi, Yixiao Liu, Yuliang Zhong, Hui Zhang, Zhonglin Wu, Boon Chin Heng, Ying Wang, Ziyang Wang, Lizi Xiao, Hua Liu, Hongwei Ouyang, Jing Zhou
Summary: This study investigates the optimal applied form and underlying mechanisms of nano-hydroxyapatite (nHAP) in bone repair. The results suggest that when nHAP is applied in the fixed state in a 3D form, it can induce optimal osteogenesis and increased bone volume through upregulation of the PI3K-AKT signaling pathway and ITGA7-mediated mechanisms, contrary to the results observed with free nHAP.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Xiaochan Li, Boon Chin Heng, Yunyang Bai, Qianqian Wang, Min Gao, Ying He, Xinwen Zhang, Xuliang Deng, Xuehui Zhang
Summary: The study confirms the dose-response effect of surface electrical charge on SHED neural differentiation and provides insights into the underlying molecular mechanisms and signaling pathways involved.
BIOACTIVE MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Engku Nasiha Engku Ngah Sayuddin, Muhammad Taher, Mohd Hafiz Arzmi, Nor Aszlitah Burhanudin, Muhamad Ashraf Rostam
Summary: This article reviews the role of podoplanin in oral cancer and its potential as a therapeutic agent. High expression of podoplanin has been observed in oral cancer and pre-cancerous lesions, suggesting that inhibitors targeting podoplanin could be effective in treating oral cancer.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Doris Haydee Rosero Salazar, Sydney Honnlee, Zi-Jun Liu
Summary: This study analyzed the dynamics and coordination of oropharyngeal muscles during natural chewing and swallowing, revealing the key role of palatal and pharyngeal muscles in chewing and the simultaneous changes in muscle activity and respiratory cycle during these processes.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Junkang Quan, Yang Liu, Lingli Ji, Yuming Zhao, Shuguo Zheng
Summary: This study identified a new DLX3 gene mutation in a Chinese family with enamel dysplasia and found that this mutation may affect ameloblast differentiation by inhibiting the transcriptional activity of Amelx/Odam/Klk4, thereby interfering with enamel formation.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Viviane Martins dos Santos Ribeiro, Mauricio Gonsalves da Costa Sousa, Paula Ribeiro Garcia, Lucas Souza dos Santos, Eliza Carla Barroso Duarte, Jose Raimundo Correa, Carlos Alberto Labate, Thais Regiani Cataldi, Rosiane Andrade da Costa, Marcelo Henrique Soller Ramada, Juliana Lott de Carvalho, Taia Maria Berto Rezende
Summary: This study compared the structural and proteomic architecture of decellularized human dental pulp using two different protocols. The results showed that the ECM preserved in the MG protocol was more intact. Proteomic analysis revealed that the MG protocol maintained around 69.56% of the proteins identified in the untreated pulp tissue, while the SG protocol only maintained 28.26%.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Jinjin Lu, Lixin Zhang, Ningxin Zhu, Dan Wang, Fei Xie, Man Qin, Yuanyuan Wang
Summary: The study investigated the effects of varying glucose concentrations on the proliferation and senescence of SHED and hBMSC. It was found that high glucose levels promoted proliferation in both cell types, but delayed senescence in SHED through autophagy inhibition and accelerated senescence in hBMSC. Additionally, the glycolysis in SHED was enhanced under high glucose culture conditions compared to hBMSC.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Luciana O. Almeida, Luan Cesar Silva, Carolina Emerick, Juliana Amorim dos Santos, Rogerio M. Castilho, Cristiane H. Squarize
Summary: This study found that the loss of PTEN signaling increases the population of HNSCC CSCs, which can be targeted by rapamycin. However, a deficiency in mTORC2 can lead to problematic selection of the parasphere CSC subtype.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Elham Mohebbi, Zainab Alimoradi, Laura S. Rozek, Azadeh Mohebbi, Elahe Jafari
Summary: This study conducted a systematic review and meta-analysis to investigate the risk of head and neck cancer among opium users. The findings showed a significant correlation between opium use and the development of head and neck cancers, particularly lip and oral cavity cancers and laryngeal cancer. This highlights the importance of further research and targeted preventive measures to address this concerning association.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Antonio Alexandre Coelho, Rute Romao Carvalho, Ana Luiza Muniz, Andre Alves Crispim, Anderson Maia Meneses, Carla Welch da Silva, Dayrine Silveira de Paula, Ana Paula Negreiros Nunes Alves, Fabricio Bitu Sousa, Paulo Goberlanio de Barros Silva
Summary: The study aimed to evaluate the role of CD20 + cells in the healing process of oral traumatic ulcers (OTU) in rats. The results showed that blocking CD20 + cells with anti-CD20 rituximab (RTX) reduced cell migration, acute inflammation, and wound healing in OTU.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Meimei Cui, Jinling Cheng, Huijuan Cheng, Ming Zhao, Dan Zhou, Min Zhang, Jingjing Jia, Limei Luo
Summary: This study explored the characteristics of human papillomavirus (HPV) in oropharyngeal carcinoma (OPC) and found that high-risk HPV infection is common in OPC patients, with higher prevalence in North America, Oceania, and Europe. Females and older patients are more susceptible to infection. Tonsil and base of tongue are the most frequent sites of infection. This study provides important theoretical basis for the prevention and treatment of OPC.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Xiaoyue Guan, Chen Shi, Yuting Wang, Yani He, Yingxue Li, Yao Yang, Wenli Mu, Wenlan Li, Tiezhou Hou
Summary: The study revealed that Gremlin1 expression levels are significantly increased in periapical lesions and may be positively correlated with IL-18 levels. Blocking Gremlin1 can significantly suppress bone resorption loss and restrain inflammatory status by affecting the activation levels of phosphorylated-CREB, ICAM-1, VCAM-1, IL-18.
ARCHIVES OF ORAL BIOLOGY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Giliano Nicolini Verzeletti, Eduardo Jose Gaio, Keilla Gomes Machado, Tobias Rauber Spuldaro, Clarissa Favero Demeda, Rafaele Frassini, Andre Felipe Streck, Sidnei Moura E. Silva, Cassiano Kuchenbecker Rosing, Joao Antonio Pegas Henriques, Mariana Roesch-Ely
Summary: This study investigates the effects of D. anceps seaweed on ligature-induced experimental periodontitis in rats. The results show that D. anceps can modulate the inflammatory response and have potential therapeutic effects for periodontitis.
ARCHIVES OF ORAL BIOLOGY
(2024)
