Journal
APPLIED SOIL ECOLOGY
Volume 124, Issue -, Pages 327-334Publisher
ELSEVIER
DOI: 10.1016/j.apsoil.2017.11.010
Keywords
T-RFLP; qPCR; Nitrification; Denitrification; Nitrogen fixation; Microbial diversity
Categories
Funding
- National Natural Science Foundation of China [81573530, 31401950, 31401306]
- Natural Science Foundation of Fujian Province [2017J01803]
- Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crop [2015-75]
- Central leading local science and technology development special fund project [2017L3010008]
- Peking University
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A field experiment with five nitrogen (N) application rates (120, 140, 160, 180, 200 kg hm(-2)) under peanut/maize intercropping regime was conducted to evaluate cropping patterns and N application rates on yield, soil enzyme activities, and rhizosphere microbial community structure. The field experiment showed that the land equivalent ratio (LER) in maize/peanut intercropping system was not decreased as compared with the mono-culture pattern, though the total N supply was approximately reduced by 20%. Terminal restriction fragment length polymorphism (T-RFLP) results showed that the treatments of intercropping (IMP100%, IMP80% and IMP60%) increased the abundance of Rhizobium hainanense, Rhizobium leguminosarum and Frankia which are associated with nitrogen fixation as compared to monoculture pattern (M100% and P100%). The qPCR results revealed that the copy number of nifH was significantly higher in the treatments of intercropping than in monoculture pattern. The result was also consistent with the T-RFLP analysis. In conclusion, peanut and maize under the condition of intercropping can promote the population of microorganisms associated with nitrogen fixing in the rhizospheric soil. Our results provide a theoretical basis and technical support to soil N utilization, development of low carbon agriculture and protection of farmland ecological environment
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