Article
Genetics & Heredity
Melissa D. Morales-Moreno, Erick G. Valdes-Galindo, Mariana M. Reza, Tatiana Fiordelisio, Jorge Peon, Armando Hernandez-Garcia
Summary: Using 2-3 gRNAs in parallel can enhance the sensitivity and speed of SARS-CoV-2 detection, accurately detecting positive and negative samples in a short time.
Review
Chemistry, Analytical
Patarajarin Akarapipad, Elizabeth Bertelson, Alexander Pessell, Tza-Huei Wang, Kuangwen Hsieh
Summary: The COVID-19 pandemic has highlighted the necessity for fast and accurate diagnostic testing. Multiplex nucleic acid diagnostic tests have made significant advancements in providing comprehensive detection of emerging variants and respiratory viruses. This review discusses the performances and limitations of different assays, provides highlights of platforms suitable for point-of-care use, and identifies technical challenges that remain. It serves as a valuable resource for future research and development in combating COVID-19.
Review
Food Science & Technology
Sandhya Sadanandan, V. S. Meenakshi, Keerthana Ramkumar, Neeraja P. Pillai, P. Anuvinda, P. J. Sreelekshmi, V Devika, K. Ramanunni, R. Jeevan Sankar, M. M. Sreejaya
Summary: Food-borne diseases caused by bacterial contamination are a serious threat to human food safety and well-being. Gold nanoparticles can be used for the detection of food-borne pathogens, ensuring food safety.
Article
Medicine, Research & Experimental
Jihyo Park, Sangwon Woo, Jiyeon Kim, Hakho Lee, Yeong-Eun Yoo, Seonki Hong
Summary: The study introduced a surface-engineered single-chamber device for nucleic acid (NA) extraction and in situ amplification, allowing rapid and convenient NA-based diagnostics. By integrating the NA extraction and amplification steps into one microfluidic chip, the system enables fast detection of pathogens. The technique offers advantages such as cost-effectiveness and ease of operation.
Article
Biophysics
Sahar Sadat Mahshid, Sarah Elizabeth Flynn, Sara Mahshid
Summary: Electrochemical biosensors offer the selectivity of electrochemical signal transducers and the specificity of biomolecular recognition strategies. During the COVID-19 pandemic, electrochemical platforms have shown potential to address significant limitations of traditional diagnostic platforms, making them strong candidates for rapid detection of SARS-CoV-2 infection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Biomedical
Sita S. Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R. Jangid, Berenice Charrez, Arturo M. Escajeda, Sungmin Son, Roger Mcintosh, Huyen Tran, Abdul Bhuiya, Maria Diaz de Leon Derby, Neil A. Switz, Maxim Armstrong, Andrew R. Harris, Noam Prywes, Maria Lukarska, Scott B. Biering, Dylan C. J. Smock, Amanda Mok, Gavin J. Knott, Qi Dang, Erik Van Dis, Eli Dugan, Shin Kim, Tina Y. Liu, Igi Testing Consortium, Erica A. Moehle, Katherine Kogut, Brenda Eskenazi, Eva Harris, Sarah A. Stanley, Liana F. Lareau, Ming X. Tan, Daniel A. Fletcher, Jennifer A. Doudna, David F. Savage, Patrick D. Hsu
Summary: This article reports a COVID-19 testing method implemented in a microfluidic device, which can rapidly detect SARS-CoV-2 RNA in saliva samples using isothermal RNA amplification and Cas13-mediated cleavage of a quenched fluorophore. The method has high sensitivity and specificity, and the testing can be completed within 60 minutes.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Chemistry, Analytical
Qiuyuan Lin, Yuanwei Cao, Guobin Han, Wen Sun, Wenhao Weng, Hui Chen, Haoyi Wang, Jilie Kong
Summary: We report a general and programmable diagnostic platform that combines mesophilic Clostridium perfringens Argonaute (CpAgo) with exponential isothermal amplification (EXPAR) for the rapid and sensitive detection of multiple miRNAs with single-nucleotide discrimination in one pot. The CpAgo-based One-Pot (COP) assay achieved a limit of detection of 1 zMmiRNA within 30 min and showed superior analytical performance in distinguishing colorectal cancer patients from healthy individuals. This programmable, one-pot, multiplex, rapid, and specific strategy holds great promise in scientific research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Pooja Bhardwaj, Rajni Kant, Sthita Pragnya Behera, Gaurav Raj Dwivedi, Rajeev Singh
Summary: This article emphasizes the importance of CRISPR/Cas technology as a diagnostic tool for early management, diagnosis, and treatment of infections and diseases, and explores its detection capabilities for non-nucleic acid targets as well as the technical challenges and improvements in clinical assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Hoang Long Pham, Hua Ling, Matthew Wook Chang
Summary: This article reviews the latest developments in microbial biosensing devices and discusses the challenges and prospects of their broad applications in real-world settings.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Owen Higgins, Terry J. Smith
Summary: PCR is crucial for infectious disease detection, especially during the COVID-19 pandemic. Real-time multiplex detection and single-base specificity play key roles in identifying single-nucleotide polymorphisms (SNPs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Qiuyuan Lin, Guobin Han, Xueen Fang, Hui Chen, Wenhao Weng, Jilie Kong
Summary: The study presents a general and programmable diagnostic strategy for miRNA analysis. By combining exponential isothermal amplification and programmable TtAgo cleavage, this method enables rapid and sensitive multiplex detection of miRNAs.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Sri Gowtham Thakku, Jackson Lirette, Kanagavel Murugesan, Julie Chen, Grant Theron, Niaz Banaei, Paul C. Blainey, James Gomez, Sharon Y. Wong, Deborah T. Hung
Summary: This study presents a new method called WATSON that can detect microbial cell-free DNA (cfDNA) for diagnosing infection. WATSON utilizes pooled amplification and CRISPR/Cas13-based detection to improve the detection of cfDNA compared to traditional methods. This approach shows potential for point-of-care diagnostic tests for infectious diseases.
NATURE COMMUNICATIONS
(2023)
Review
Biotechnology & Applied Microbiology
Kun Chen, Ziyi Shen, Guanzhen Wang, Wei Gu, Shengchao Zhao, Zihan Lin, Wei Liu, Yi Cai, Gohar Mushtaq, Jia Jia, Chunpeng (Craig) Wan, Tingdong Yan
Summary: CRISPR/Cas technology, awarded the Nobel Prize in Chemistry in 2020, has gained attention for its success in gene editing and its potential in molecular diagnostics. By integrating with biosensors and bioassays, CRISPR-based detection shows high specificity and sensitivity, making it a promising tool for detecting biomarkers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Maria-Carmen Marques, Javier Sanchez-Vicente, Rau'l Ruiz, Roser Montagud-Martinez, Rosa Marquez-Costa, Gustavo Gomez, Alberto Carbonell, Jose-Antonio Daros, Guillermo Rodrigo
Summary: This article introduces a method for rapid detection of viruses in plants using CRISPR technology. By utilizing the CRISPR-Cas12a and CRISPR-Cas13a/d systems, different RNA viruses can be detected, and real-time detection can be performed in field conditions.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
M. Dong, C. Fisher, G. Anez, M. Rios, H. L. Nakhasi, J. P. Hobson, M. Beanan, D. Hockman, E. Grigorenko, R. Duncan
JOURNAL OF APPLIED MICROBIOLOGY
(2016)
Article
Microbiology
Ashutosh, Mansi Garg, Shyam Sundar, Robert Duncan, Hira L. Nakhasi, Neena Goyal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Article
Immunology
Ranadhir Dey, Pradeep K. Dagur, Angamuthu Selvapandiyan, J. Philip McCoy, Poonam Salotra, Robert Duncan, Hira L. Nakhasi
JOURNAL OF IMMUNOLOGY
(2013)
Article
Immunology
Hamide Aslan, Ranadhir Dey, Claudio Meneses, Philip Castrovinci, Selma Maria Bezerra Jeronimo, Gaetano Oliva, Laurent Fischer, Robert C. Duncan, Hira L. Nakhasi, Jesus G. Valenzuela, Shaden Kamhawi
JOURNAL OF INFECTIOUS DISEASES
(2013)
Article
Pathology
Elena Grigorenko, Carolyn Fisher, Sunali Patel, Caren Chancey, Maria Rios, Hira L. Nakhasi, Robert C. Duncan
JOURNAL OF MOLECULAR DIAGNOSTICS
(2014)
Editorial Material
Pathology
Robert Duncan
JOURNAL OF MOLECULAR DIAGNOSTICS
(2014)
Article
Biochemistry & Molecular Biology
Sreenivas Gannavaram, Patricia S. Connelly, Mathew P. Daniels, Robert Duncan, Poonam Salotra, Hira L. Nakhasi
MOLECULAR MICROBIOLOGY
(2012)
Article
Multidisciplinary Sciences
Sreenivas Gannavaram, Paresh Sharma, Robert C. Duncan, Poonam Salotra, Hira L. Nakhasi
Article
Infectious Diseases
Ines Lakhal-Naouar, Armando Jardim, Rona Strasser, Shen Luo, Yukiko Kozakai, Hira L. Nakhasi, Robert C. Duncan
PLOS NEGLECTED TROPICAL DISEASES
(2012)
Article
Infectious Diseases
Sreenivas Gannavaram, Sonya Davey, Ines Lakhal-Naouar, Robert Duncan, Hira L. Nakhasi
PLOS NEGLECTED TROPICAL DISEASES
(2014)
Review
Pathology
Robert Duncan, Elena Grigorenko, Carolyn Fisher, Donna Hockman, Bryan Lanning
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2019)
Article
Biotechnology & Applied Microbiology
R. A. Multari, D. A. Cremers, A. Nelson, Z. Karimi, S. Young, C. Fisher, R. Duncan
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Irina Tiper, Moussa Kourout, Carolyn Fisher, Krishnamurthy Konduru, Anjan Purkayastha, Gerardo Kaplan, Robert Duncan
Summary: Filoviruses are emerging pathogens causing acute fever with high fatality rates, posing a global public health threat. Genome sequencing during the Ebola virus outbreak enabled the study of virus evolution, pathogenic mutations, and viral spread. Resequencing microarrays provide a targeted, rapid, and sensitive method for obtaining genomic sequences critical in studying Ebolaviruses.
Review
Public, Environmental & Occupational Health
Angamuthu Selvapandiyan, Ranadhir Dey, Sreenivas Gannavaram, Ines Lakhal-Naouar, Robert Duncan, Poonam Salotra, Hira L. Nakhasi
JOURNAL OF TROPICAL MEDICINE
(2012)