Article
Infectious Diseases
Ivana Cirkovic, Bruno H. Muller, Ana Janjusevic, Patrick Mollon, Valerie Istier, Caroline Mirande-Meunier, Snezana Brkic
Summary: This study investigated the occurrence and characteristics of carbapenem-resistant Gram-negative bacteria (CR-GNB) in wastewater in Belgrade. All isolates were found to be multidrug-resistant, with K. pneumoniae (bla(CTX-M-15)/bla(OXA-48)) being the most common. Additionally, acquired resistance to colistin was detected in some isolates. Further research is needed to track the occurrence and distribution of these bacteria and mitigate their threat.
Article
Microbiology
Yasmine H. Tartor, Norhan K. Abd El-Aziz, Rasha M. A. Gharieb, Hend M. El Damaty, Shymaa Enany, Enas A. Soliman, Samah S. Abdellatif, Amira S. A. Attia, Mosa M. Bahnass, Yousry A. El-Shazly, Mohammed Elbediwi, Hazem Ramadan
Summary: This study investigated the prevalence and antimicrobial resistance of Gram-negative bacteria isolated from clinical and subclinical bovine mastitis, and raw unpasteurized milk. The findings revealed high levels of multidrug-resistant species such as Klebsiella pneumoniae, Aeromonas hydrophila, Enterobacter cloacae, Escherichia coli, and Proteus mirabilis. The emergence of K. pneumoniae co-harboring mcr-10 and fosA5 genes in bovine milk in the Middle East poses a public health threat.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Haichen Wang, Jia Li, Changhang Min, Fengjun Xia, Mengli Tang, Jun Li, Yongmei Hu, Mingxiang Zou
Summary: This study investigated the silver resistance mechanisms and coexistence of antibiotic resistance genes with the sil operon among gram-negative pathogens isolated from wound samples. The results showed a prevalent cryptic silver resistance among Enterobacteriaceae with the sil operon or in combination with the cus operon and major porin loss, along with the presence of antibiotic resistance genes.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Yuan Liu, Kangni Yang, Yuqian Jia, Jingru Shi, Ziwen Tong, Zhiqiang Wang
Summary: The study showed that thymine can effectively enhance the bactericidal activity of antibiotics against Gram-negative pathogens by activating bacterial metabolism and restoring susceptibility to antibiotics. This finding provides a universal strategy to overcome Gram-negative pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Claire Maher, Karl A. Hassan
Summary: Gram-negative bacteria are resistant to antibiotics due to their cell envelope's permeability barrier. Understanding the role of different components in the envelope is crucial for developing new broad-spectrum antibiotics.
Article
Infectious Diseases
Omar Ahmed
Summary: This study evaluated the presence of antibiotic resistance genes in clinical strains of P. aeruginosa using whole-genome sequencing. The results showed that resistant strains had multiple antibiotic resistance genes and exhibited resistance to different types of antibiotics. These findings contribute to the understanding of the development of multidrug-resistant P. aeruginosa.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Jaime E. Zlamal, Semen A. Leyn, Mallika Iyer, Marinela L. Elane, Nicholas A. Wong, James W. Wamsley, Maarten Vercruysse, Fernando Garcia-Alcalde, Andrei L. Osterman
Summary: Resistance to the broad-spectrum antibiotic ciprofloxacin is detected at high rates in various bacterial pathogens, with early resistance primarily caused by single mutations in DNA gyrase target genes. Further genetic alterations influence drug efflux mechanisms and secondary target genes, leading to increased resistance levels. The study highlights shared and unique evolutionary aspects across different species, providing insights for the development of novel antibiotics with reduced resistance tendencies.
Review
Microbiology
Manon Lang, Andre Carvalho, Zeynep Baharoglu, Didier Mazel
Summary: This review aims to consolidate existing and recent knowledge about the uptake of aminoglycosides (AGs), explore the connection between AG-dependent bacterial stress and drug uptake, and present new strategies to enhance AG uptake for more efficient antibiotic therapies, with a focus on the connection between sugar transport and AG potentiation.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Immunology
Lenka Davidova-Gerzova, Jarmila Lausova, Iva Sukkar, Kristina Nesporova, Lucie Nechutna, Katerina Vlkova, Katerina Chudejova, Marcela Krutova, Jana Palkovicova, Jakub Kaspar, Monika Dolejska
Summary: This study revealed the transmission routes of antibiotic-resistant bacteria between hospitals and wastewater treatment plants. The results showed that Escherichia coli from urinary tract infections in hospitalized patients can enter the environment through wastewater, and they have extended-spectrum beta-lactamase (ESBL) and multidrug resistance (MDR) characteristics. Additionally, the transmission of bacteria with different sequence types through wastewater treatment plants to surface waters further exacerbates the spread of antibiotic resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Nathalia Diogo Trocado, Marcelo Soares de Moraes, Lilian Aveleda, Cristiane Rodrigues Silva, Victor Augustus Marin
Summary: The study detected multidrug resistance and various resistance genes in fresh fruit juice samples served to patients in hospitals, highlighting the potential risks of providing these foods to patients.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Infectious Diseases
Belen Fernandez-Caso, Ana Miqueleiz, Teresa Alarcon
Summary: Antibiotic resistance (AMR) is a worldwide concern, and Helicobacter pylori is no exception. Increasing resistance to antibiotics leads to higher treatment failure rates. Next-generation sequencing-based molecular tools, such as whole genome sequencing (WGS), can be valuable for understanding the genomic mechanisms of resistance and optimizing treatment guidelines based on local epidemiology. Various pipelines and platforms are continuously being developed for analyzing WGS data. This study provides a detailed view of the use and progression of WGS for studying H. pylori's AMR.
Article
Microbiology
Widad Chelaghma, Lotfi Loucif, Mourad Bendahou, Jean-Marc Rolain
Summary: Antibacterial resistance is a major threat to global public health, with the emergence of resistance among Gram-negative bacteria being a serious concern. These bacteria are increasing not only in hospital settings but also in other sources like vegetables and fruit. Fresh produce, popular for its consumption in healthy diets, can also serve as a source of foodborne disease and a reservoir for antibiotic resistant Gram-negative bacteria.
Article
Immunology
Natasha Spottiswoode, Samantha Hao, Estella Sanchez-Guerrero, Angela M. Detweiler, Honey Mekonen, Norma Neff, Henriette Macmillan, Brian S. Schwartz, Joanne Engel, Joseph L. Derisi, Steven A. Miller, Charles R. Langelier
Summary: This study used whole genome sequencing (WGS) to investigate recurrent Pseudomonas aeruginosa bloodstream infections in severely immunocompromised patients. The infections showed progressive increases in resistance to beta lactam antibiotics, despite appropriate treatment. WGS analysis revealed the in-host evolution of a single P. aeruginosa isolate with acquired genetic resistance mechanisms over the course of treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Ayan Mukherjee, Danyel Ramirez, Rajat Arora, Gilbert Arthur, Frank Schweizer
Summary: This study describes the synthesis and biological properties of amphiphilic tribasic galactosamines as potential rifampicin (RIF) potentiators. The results demonstrate that these compounds enhance the effectiveness of RIF against multidrug-resistant Acinetobacter baumannii and Escherichia coli in low salt-containing media, but not against Pseudomonas aeruginosa. The potentiating effect of RIF was reduced when bivalent Mg++ or Ca++ ions were added at physiological concentrations.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Microbiology
Tian Deng, Yuqian Jia, Ziwen Tong, Jingru Shi, Zhiqiang Wang, Yuan Liu
Summary: Antibiotic resistance poses a serious threat to public health and human safety. The emergence of the novel resistance gene tet(X4) and its variants has jeopardized the clinical utility of tigecycline, a last-line antibiotic for multidrug-resistant bacterial infections. However, the combination of bismuth drugs, particularly bismuth nitrate [Bi(NO3)(3)], with tigecycline has shown to effectively enhance its antibacterial activity against tet(X)-positive bacteria. This is achieved by inhibiting the enzymatic activity of the Tet(X) protein through competitive and noncompetitive binding mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Jinane Tabcheh, Julia Vergalli, Anne Davin-Regli, Noha Ghanem, Jean-Marie Pages, Charbel Al-Bayssari, Jean Michel Brunel
Summary: Antibiotic resistance is increasing morbidity and mortality for non-curable infectious diseases. Novel strategies, such as effective antibiotic combinations and antibiotics with non-antibiotic activity-enhancing compounds, have emerged to combat multidrug-resistant microorganisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Mohamad Yasmin, Laura J. Rojas, Steven H. Marshall, Andrea M. Hujer, Anna Cmolik, Emma Marshall, Helen W. Boucher, Alejandro J. Vila, Maxime Soldevila, Seydina M. Diene, Jean-Marc Rolain, Robert A. Bonomo
Summary: This study characterized the drug resistance mechanisms and evaluated the efficacy of contemporary antibiotics against Elizabethkingia anophelis, an emerging pathogen causing life-threatening infections in immunocompromised patients. Whole-genome sequencing identified drug-resistant genes and determined the susceptibility of the pathogen to different antibiotics. The study provides important guidance for selecting appropriate antimicrobial therapy.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Khouloud Slimene, Allaaeddin El Salabi, Olfa Dziri, Najla Mathlouthi, Seydina M. Diene, Elhussan Ahmed Mohamed, Jadalla M. A. Amhalhal, Mohammed O. Aboalgasem, Jomaa F. Alrjael, Jean-Marc Rolain, Chedly Chouchani
Summary: Antimicrobial resistance, especially to carbapenems, poses a major threat to public health. A study conducted in Ibn Sina Hospital in Libya identified low susceptibility to carbapenems among Gram-negative bacteria. Molecular investigations revealed the presence of New Delhi metallo-beta-lactamases-1 and Verona integron-encoded metallo-beta-lactamase enzymes in Pseudomonas spp. Additionally, a new gene and resistance gene were discovered in Acinetobacter baumannii.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Lilia Messadi, Jean-Marc Rolain
Summary: A study conducted in Tunisia found that rodents can potentially transmit multidrug resistant bacteria, posing a public health problem. The researchers isolated Enterobacteriaceae strains from the intestines of rats captured from various locations and found that rodents may play a role in the spread of antimicrobial resistant E. coli.
VETERINARY MICROBIOLOGY
(2023)
Editorial Material
Microbiology
Ziad Daoud, Jean-Marc Rolain
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Khouloud Slimene, Asrra A. Ali, Elhussan A. Mohamed, Allaaeddin El Salabi, Faraj S. Suliman, Agela A. Elbadri, Fadi F. El-fertas, Ahmed El-awjly, Salah A. Shokri, Jean-Marc Rolain, Chedly Chouchani
Summary: The emergence of carbapenem-resistant bacteria in SARS-CoV-2 patients in eastern Libya highlights the need for increased surveillance and rational use of antibiotics, particularly carbapenems, during the pandemic. Multidrug-resistant strains, including K. pneumoniae, A. baumannii, and colistin-resistant E. coli, were identified, emphasizing the importance of maintaining the efficacy of these antibiotics and minimizing their overuse.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Sabah El-Sawalhi, Oceane Revol, Amanda Chamieh, Alexandre Lacoste, Alexandre Annessi, Bernard La Scola, Jean-Marc Rolain, Isabelle Pagnier
Summary: This study examines the circulation of bacterial strains and their antibiotic resistance genes in the aquatic environment in Marseille, France. The importance of monitoring the frequency of these circulating bacteria in water treatments is emphasized.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Mohamad Maatouk, Jean-Marc Rolain, Fadi Bittar
Summary: Microbial ecology is a critical field that studies the composition, diversity, and functions of microorganisms in different environments and health-related processes. The discovery of Candidate Phyla Radiation (CPR) has brought attention to a new group of microorganisms characterized by symbiotic/parasitic lifestyle, small cell size, and small genome. Although poorly understood, CPRs have been widely detected in various environmental and clinical samples and are found to exhibit high genetic diversity. This review provides an overview of CPR discovery and highlights their genomic characteristics and interactions with other microbes in different ecological niches.
Letter
Infectious Diseases
May Khoder, Marwan Osman, Issmat I. Kassem, Rayane Rafei, Ahmad Shahin, Seydina M. Diene, Jean-Marc Rolain, Monzer Hamze
NEW MICROBES AND NEW INFECTIONS
(2023)
Correction
Public, Environmental & Occupational Health
Thi Loi Dao, Van Thuan Hoang, Amal Magmoun, Tran Duc Anh Ly, Sophie Alexandra Baron, Linda Hadjadj, Naomie Canard, Tassadit Drali, Frederique Gouriet, Didier Raoult, Philippe Parola, Pierre Marty, Jean-Marc Rolain, Philippe Gautret
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Review
Cell Biology
Seydina M. Diene, Pierre Pontarotti, Said Azza, Nicholas Armstrong, Lucile Pinault, Eric Chabriere, Philippe Colson, Jean-Marc Rolain, Didier Raoult
Summary: Beta-lactamase enzymes are capable of conferring resistance to widely used antibiotics in human medicine, making them a topic of significant interest. Among these enzymes, the class B beta-lactamases are a type of metallo-beta-lactamase fold protein found not only in bacteria but also in other organisms. Previously unexplored in other organisms due to various barriers, modern search approaches have now uncovered their presence in different organisms including Bacteria, Archaea, Nanoarchaeota, Asgard, Humans, Giant viruses, and Candidate Phyla Radiation (CPR). These multifunctional enzymes exhibit diverse enzymatic or non-enzymatic activities, with at least thirteen reported activities such as beta-lactamase, ribonuclease, nuclease, glyoxalase, lactonase, phytase, ascorbic acid degradation, anti-cancer drug degradation, or membrane transport. This review discusses the existence of M beta L fold enzymes in different domains of life, presents suitable approaches for studying their homologous sequences in unexpected sources, and presents described M beta L fold enzymes with demonstrated enzymatic or non-enzymatic activities.
Article
Microbiology
Mohamad Maatouk, Vicky Merhej, Pierre Pontarotti, Ahmad Ibrahim, Jean-Marc Rolain, Fadi Bittar
Summary: The Candidate Phyla Radiation (CPR) harbors a large number of genes encoding enzymes with potential antibiotic resistance activity, particularly metallo-beta-lactamase-like (MBL-like) enzymes. These CPR MBL-like sequences are longer and more diverse than bacterial MBLs, and they have a high content of functional domains. Most CPR MBL-like sequences have not been identified with known MBLs, indicating the presence of many potential, yet undiscovered, functions in CPR.
Article
Microbiology
Habibou Sarr, Aissatou Ahmet Niang, Amadou Diop, Oleg Mediannikov, Hanane Zerrouki, Seydina M. Diene, Seynabou Lo, Mouhamadou Lamine Dia, Ahmad Iyane Sow, Florence Fenollar, Jean-Marc Rolain, Linda Hadjadj, Carla Calia, Lawrence S. Young
Summary: This study investigated the antibiotic resistance of enterobacteria in Senegal and identified the emergence of carbapenemase-producing Enterobacterales (CPE) infections. The results showed that carbapenemase genes and class A β-lactamase genes were widely present in enterobacteria. This phenomenon is concerning, and strict surveillance is necessary to prevent further spread.
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
