Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 24, Pages 7019-7022Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201713080
Keywords
calcium signaling; ion channels; optogenetics; protein engineering; synthetic biology
Categories
Funding
- NIH [R21GM126532, R01GM112003]
- CPRIT [RP170660, RR140053]
- National Natural Science foundation of China [NSFC-31471279, NSFC-81728019]
- Recruitment Program for Young Professionals of China
- Fundamental Research Funds for the Central Universities [2017STUD20]
- Welch Foundation [BE-1913]
- American Cancer Society [RSG-16-215-01-TBE]
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Voltage-gated Ca2+ (Ca-V) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca-V channel modulators, to enable photo-tunable modulation of Ca-V channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca-V channels could find broad applications in interrogating a wide range of Ca-V-mediated physiological processes.
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