Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Chemistry, Applied
Suying Li, Tsz-Tsun Ng, Zhong-Ping Yao
Summary: This study developed a new method using MALDI-MS and PLS-R to quantitate triacylglycerols in blended oils, allowing simultaneous quantitation of multiple compositions with good limits of detection. The new method enables direct analysis of blended oils, rapid analysis of samples, and accurate quantitation of low-abundance oil compositions and blended oils with similar fatty acid contents, compared to conventional methods.
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Nanoscience & Nanotechnology
Peiqi Luo, Liping Wang, Lixia Jiang, Jie Sun, Yafeng Li, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: Graphdiyne, a new carbon nanomaterial, has been utilized as a matrix in laser desorption ionization mass spectrometry for small molecule analysis, showing enhanced desorption and ionization efficiency. The use of Graphdiyne in LDI MS analysis can potentially improve the early diagnosis of liver cancer by identifying fatty acids as potential biomarkers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Gabriel R. Martins, Felipe L. Brum, Davi Marconi Miranda Carvalho da Silva, Livia C. Barbosa, Ronaldo Mohana-Borges, Ayla Sant'Ana da Silva
Summary: Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a valuable technique for identifying compounds and understanding their physiological information in plant tissues. Researchers have developed a sample preparation protocol to obtain thin sections of hard seeds, enabling molecular mapping by MALDI-IMS. This method has the potential to be a valuable tool for studying the morphology, anatomy, and physiology of botanical materials.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Spectroscopy
Wendy H. Mueller, Alexandre Verdin, Edwin De Pauw, Cedric Malherbe, Gauthier Eppe
Summary: Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a technique that uses nanostructured substrates to promote the desorption/ionization of analytes, allowing for both untargeted and targeted analysis. This review focuses on SALDI-MS imaging (SALDI-MSI), discussing the nomenclature, fundamental mechanisms, analytical strategies, and applications in various fields. SALDI-MSI has strengths and limitations, but shows promising prospects for future development.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Yajie Ding, Congcong Pei, Weikang Shu, Jingjing Wan
Summary: Metabolic analysis in biofluids plays a crucial role in disease diagnosis and monitoring, and LDI MS-based metabolic analysis shows great potential in this field. This review discusses the essential elements of LDI MS-based metabolic analysis and summarizes the recent developments in diagnostic applications using different nanomaterials and machine learning algorithms.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Review
Chemistry, Physical
Wen Ma, Jun Li, Xianjiang Li, Yu Bai, Huwei Liu
Summary: The rapid advancement of nanotechnology in the field of SALDI-MS has led to the development of novel nanoparticles and nanostructured substrates, improving detection sensitivity and specificity, and impacting the field of biomedical analysis significantly.
Article
Oncology
Ibuki Ueoka, Akari Takai, Mizuki Yamaguchi, Tomohiro Chiyonobu, Hideki Yoshida, Masamitsu Yamaguchi
EXPERIMENTAL CELL RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Steven J. Marygold, Helen Attrill, Elena Speretta, Kate Warner, Michele Magrane, Maria Berloco, Sue Cotterill, Mitch McVey, Yikang Rong, Masamitsu Yamaguchi
Article
Biochemistry & Molecular Biology
Hang Thi Nguyet Pham, Hong Nguyen Tran, Phuong Thi Nguyen, Xoan Thi Le, Khoi Minh Nguyen, Sinh Viet Phan, Masanori Yoneyama, Kiyokazu Ogita, Taro Yamaguchi, William R. Folk, Masamitsu Yamaguchi, Kinzo Matsumoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Salinee Jantrapirom, Yosuke Enomoto, Jirarat Karinchai, Mizuki Yamaguchi, Hideki Yoshida, Eiichiro Fukusaki, Shuichi Shimma, Masamitsu Yamaguchi
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Masamitsu Yamaguchi, Kentaro Omori, Satoshi Asada, Hideki Yoshida
Summary: ALS and CMT are common neurodegenerative disorders with significant phenotypic heterogeneity, potentially due to modifiers that influence disease characteristics. Epigenetic regulation of biological functions through gene expression may be an important mechanism in these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Eric Hyrmeya Savadogo, Yui Shiomi, Junko Yasuda, Toshiharu Akino, Masamitsu Yamaguchi, Hideki Yoshida, Takanari Umegawachi, Ryo Tanaka, Dang Ngoc Anh Suong, Kenji Miura, Kazufumi Yazaki, Sakihito Kitajima
Summary: Genes of the PLAT protein family play a role in plant defense against insects through the involvement of anti-microbe or anti-insect proteins in laticifer cells. These genes induce an indirect insect resistance response in the host plant, as well as possibly causing direct cytotoxic effects on insects. Larval growth of insects is significantly affected by the expression of PLAT proteins from different plant species, highlighting the conserved anti-insect activity of the PLAT protein family in the plant kingdom.
Article
Biochemistry & Molecular Biology
Hang Thi Nguyet Pham, Hong Nguyen Tran, Xoan Thi Le, Ha Thi Do, Tue Trong Nguyen, Chien Le Nguyen, Hideki Yoshida, Masamistu Yamaguchi, Folk R. William, Kinzo Matsumoto
Summary: This study found that a chemically characterized extract from Ilex kudingcha can improve behavioral traits in a Drosophila melanogaster rugose mutant, resembling human Autism Spectrum Disorder (ASD) traits. The extract also increased synaptic bouton number in Drosophila rugose and altered gene expression in the mouse hippocampus. These results suggest the potential of Ilex kudingcha extract as a treatment for some forms of ASD.
NEUROCHEMICAL RESEARCH
(2021)
Article
Neurosciences
Yuuka Muraoka, Atsushi Nikaido, Ryosuke Kowada, Hiroshi Kimura, Masamitsu Yamaguchi, Hideki Yoshida
Summary: Mutations in the FIG 4 gene are associated with various disorders, possibly due to dysregulated epigenetics. HDAC1 has been identified as an important epigenetic regulator of FIG 4, suggesting potential therapeutic targets for FIG 4-related disorders such as CMT.
Correction
Oncology
Hisanori Matsubara, Reiji Tanaka, Tatsuya Tateishi, Hideki Yoshida, Masamitsu Yamaguchi, Takao Kataoka
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Oncology
Masamitsu Yamaguchi, Im-soon Lee, Salinee Jantrapirom, Kojiro Suda, Hideki Yoshida
Summary: Drosophila is being used as a model for studying human diseases, with functional homologues of a large percentage of human disease-related genes found in Drosophila. This review discusses Drosophila models targeting genes associated with ALS and CMT, highlighting potential therapeutic targets and diagnostic markers.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Oncology
Ngoc-Lan Thi Nguyen, Ngoc-Dzung Thi Dang, Quang-Huy Dang, Van-Chuc Tran, Hoang-Long Vo, Masamitsu Yamaguchi, Thanh-Van Ta
Summary: The study found that the polymorphisms of Mucin 1 gene, specifically rs4072037 and rs2070803, are associated with gastric cancer risk, with the AA genotype potentially increasing risk while the AG genotype may lower it. Combining factors such as age, gender, alcoholism, and personal history of gastric disease significantly elevate the risk of gastric cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Ryosuke Kowada, Atsushi Kodani, Hiroyuki Ida, Masamitsu Yamaguchi, Im-Soon Lee, Yasushi Okada, Hideki Yoshida
Summary: The homolog of human SCO2, Scox, plays an essential role in mitochondrial function and locomotive behavior in Drosophila. Knockdown of Scox in glial cells impairs mitochondrial morphology and function, as well as locomotive behavior, mimicking the pathological phenotypes observed in humans with mutations in the SCO2 gene.
SCIENTIFIC REPORTS
(2021)
Correction
Multidisciplinary Sciences
Tomoki Hirashima, Ryo Tanaka, Masamitsu Yamaguchi, Hideki Yoshida
SCIENTIFIC REPORTS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Masamitsu Yamaguchi, Shinya Yamamoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Yu Hiramatsu, Yuji Okamoto, Akiko Yoshimura, Jun-Hui Yuan, Masahiro Ando, Yujiro Higuchi, Akihiro Hashiguchi, Eiji Matsuura, Fumihito Nozaki, Tomohiro Kumada, Kei Murayama, Mikiya Suzuki, Yuki Yamamoto, Naoko Matsui, Yoshimichi Miyazaki, Masamitsu Yamaguchi, Youji Suzuki, Jun Mitsui, Hiroyuki Ishiura, Masaki Tanaka, Shinichi Morishita, Ichizo Nishino, Shoji Tsuji, Hiroshi Takashima
Summary: This study identified causative mutations in mitochondrial-related nuclear genes in suspected hereditary peripheral neuropathy patients. Whole-exome sequencing was performed on a large cohort of Japanese patients, and novel pathogenic variants were detected in four mitochondrial-related nuclear genes. These patients exhibited sensory and motor axonal polyneuropathy, along with central nervous system or multisystem involvements.
JOURNAL OF NEUROLOGY
(2022)
