Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 410, Issue 20, Pages 4867-4873Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1127-2
Keywords
Whole blood; Antibodies; Plasma; Serum; Asymmetric flow field-flow fractionation (AF4); Fluorescence labelling
Funding
- Vinnova Competence Center NextBioForm
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The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.
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