Article
Chemistry, Analytical
Hong Wang, Guangmei Han, Hesen Tang, Ruilong Zhang, Zhengjie Liu, Yingqiang Sun, Bianhua Liu, Junlong Geng, Zhongping Zhang
Summary: Fluorescence microscopy is an important tool in cell biology research, but its use for long-term and strong-illumination imaging of live cells is limited by photobleaching and phototoxicity. This study introduces a new concept of fluorophore engineering chemistry, using synchronous photoactivation-imaging (SPI) fluorophores to overcome these limitations. The SPI fluorophores allow continuous replenishing of bright-state probes and reduce photocytotoxicity, enabling long-term imaging of live cells under intensive illumination.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Su Liang, Zeyu Wang, Luyu Qi, Chao Tang, Yiming Zhang, Qun Luo, Yayun Wu, Jinghe Yuan, Yao Zhao, Yanyan Zhang, Xiaohong Fang, Shijun Wang, Fuyi Wang
Summary: This study designed a fluorescent probe, ATTO565-WGN, which showed similar anti-proliferative activity to wogonin against human cancer cell lines and demonstrated mitochondrial localization through confocal fluorescence imaging. The conjugated ATTO565 fluorophore enabled STED fluorescence living cell imaging with ultrahigh spatial resolution, facilitating the investigation of wogonin-biological target interactions in living cells at mitochondria. This work provides novel insights into rational design of mitochondrion targeting fluorescence probes for ultrahigh resolution living cell imaging.
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Zhenzhen Lu, Luke W. Giles, Rico F. Tabor, Boon Mian Teo
Summary: The study fabricated NE-derived CDs with green luminescence, showing pH and concentration dependent fluorescence behavior. The multiple functional groups on the CDs allow them to alter fluorescence intensity and peak position in different pH conditions, demonstrating significant potential for live-cell imaging and blood sample detection. Additionally, a simple and effective method based on the inner filter effect was developed to determine the concentration of hemoglobin in diluted human blood samples, showing good linearity and a low limit of determination.
Article
Multidisciplinary Sciences
Alice Sandmeyer, Lili Wang, Wolfgang Huebner, Marcel Mueller, Benjamin K. Chen, Thomas Huser
Summary: Cost-effective, highly portable, and easy to use high-resolution live-cell imaging systems can revolutionize research in challenging environments, enabling real-time tracking of virus particles and studying infection mechanisms.
Article
Chemistry, Analytical
Kyong Tkhe Fam, Remi Pelletier, Farah Bouhedda, Michael Ryckelynck, Mayeul Collot, Andrey S. Klymchenko
Summary: This study aims to develop a robust molecular tool for intracellular RNA imaging. By rational design, a fluorogenic self-quenched dimer was successfully developed, which showed good affinity and fluorescence response to its cognate aptamer both in solution and live cells, with a better limit of detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Yujie Zheng, Yean Jin Lim, Hanqi Lin, Tienan Xu, Carmen Longbottom, Viviane Delghingaro-Augusto, Yee Lin Thong, Christopher R. Parish, Elizabeth E. Gardiner, Woei Ming Lee
Summary: This paper introduces a method to study cell migration using live cell imaging tools and proposes a combined system that integrates label-free nanoscale and fluorescence imaging. By empirically determining optimal scanning angles, quantitative 3D spatial temporal details of cellular components can be obtained. Using this combined imaging tool, researchers analyzed the migration of platelets and endothelial cells and made key findings, such as the importance of bovine serum albumin in platelet migration and the dynamics of mitochondria during cell migration.
Article
Chemistry, Applied
Yin Ai, Haichang Ding, Congbin Fan, Gang Liu, Shouzhi Pu
Summary: In this study, a fluorescent probe named DTRN was synthesized for the detection and imaging of Cysteine and Homocysteine in living systems. DTRN exhibited excellent sensitivity, specificity, and fast response, and it was also capable of detecting Cysteine in HeLa cells.
Article
Chemistry, Analytical
Jian Lv, Shuai Chang, Xiaoyuan Wang, Zerui Zhou, Binbin Chen, Ruocan Qian, Dawei Li
Summary: The study presents a highly efficient and convenient two-step tagging strategy for live-cell profiling of membrane sialic acids using fluorescence imaging and SERS labeling. By using tumor markers as model proteins, sialic acids can be partitioned into distinct space domains, allowing for classification of various types of cells. This strategy provides a robust and versatile platform for highly detailed analysis of cell surface saccharide profiles, with potential applications in optical biosensing and molecular diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Anayat Bhat, Shuang Li, Daniel Hammler, Martin J. Winterhalder, Andreas Marx, Andreas Zumbusch
Summary: The hydrolysis of nucleotides is important for cellular energy, and the transfer of phosphates to proteins is crucial for post-translational modification. A novel fluorogenic adenosine 5'-tetraphosphate (Ap4) analog has been developed for real-time monitoring of ATP hydrolysis within live cells, with potential applications in studying cellular processes involving Ap4 hydrolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Simon Pascal, San-Hui Chi, Alexei Grichine, Veronique Martel-Frachet, Joseph W. Perry, Olivier Maury, Chantal Andraud
Summary: This study reports the synthesis and optical properties investigation of amino-heptamethines, providing new insights into the two-photon absorption of this type of chromophores and exploring their potential for bio-imaging of living cells.
Article
Chemistry, Multidisciplinary
Esra Tanriverdi Ecik, Emrah Ozcan, Hasan Huseyin Kazan, Ismail Erol, Elif Senkuytu, Bunyemin Cosut
Summary: This study introduces a novel triad system comprising subphthalocyanine, BODIPY, and thiophene/thianaphthene units, with optical properties analyzed through various spectroscopic methods and evaluation in breast cancer cell imaging. The triads showed dual color emission, demonstrating significance in biomedical applications.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Naishun Liao, Lichao Su, Youshi Zheng, Bixing Zhao, Ming Wu, Da Zhang, Huanghao Yang, Xiaolong Liu, Jibin Song
Summary: The study introduces a novel ratiometric NIR-II fluorescence imaging strategy to track and visualize NK cell viability in vivo, demonstrating that co-treatment with IL-2, IL-15, and IL-21 can enhance NK cell viability and improve immunotherapy for hepatocellular carcinoma. This approach allows for longitudinal and quantitative monitoring of NK cell viability in NK cell-based immunotherapy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Biomedical
Yaxi Li, Dehong Hu, Zonghai Sheng, Tianliang Min, Menglei Zha, Jen-Shyang Ni, Hairong Zheng, Kai Li
Summary: In recent decades, research on fluorogens with aggregation-induced emission (AIEgens) has been intensively conducted with a focus on biomedical applications. One efficient strategy involves designing amphiphilic AIEgens that can self-assemble into uniform nanoparticles for high-resolution intravital vascular fluorescence imaging with a significant penetration depth.
Article
Materials Science, Multidisciplinary
Xiaolu Zhou, Xue Bai, Xuejian Zhang, Jing Wu, Yu Liu
Summary: A macrocyclic confinement-activated green phosphorescence and two-photon near-infrared (NIR) fluorescence supramolecular cascade assembly has been constructed by multivalent interaction. Through the use of CB[8]-induced macrocyclic confinement and molecular folding, the fluorescence of BTPA is effectively redshifted, and NIR fluorescence is enhanced through cascade confinement. Introduction of the PY/CB[8] complex leads to the fabrication of multicomponent nanoparticles, showing efficient NIR fluorescence and green phosphorescence emission for two-photon cancer cell-targeted imaging.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Chemistry, Applied
Le Liu, Rongjin Zeng, Jiawei Jiang, Tiancheng Wu, Peisheng Zhang, Chonghua Zhang, Jiaxi Cui, Jian Chen
Summary: The study introduces a novel multi-state photoswitchable fluorescent polymeric nano-particles that can switch between different colors by adjusting the wavelength of light stimuli. These nanoparticles exhibit excellent performance, such as good water dispersibility, high brightness, fast responsiveness, and good photoreversibility.
Editorial Material
Chemistry, Physical
Jin Ma, Xi Xiao, Lijie Luo, Jiaming Liu, Yuanqiang Hao, Peisheng Zhang, Rongjin Zeng, Shu Chen
Summary: This paper reported the synthesis and application of polymelamine (pMel) in deep eutectic solvents (DES) for dopamine sensing. The first attempt to electrosynthesize pMel in a DES system was a valuable contribution, but there are some missing points that may lead to errors in the analysis of cyclic voltammetry and make it difficult to synthesize pMel under the described conditions.
Article
Nanoscience & Nanotechnology
Jia Tang, Yong Tian, Zhong Lin, Chonghua Zhang, Peisheng Zhang, Rongjin Zeng, Si Wu, Xudong Chen, Jian Chen
Summary: In this study, novel photoswitchable multistate fluorescent supramolecular polymers (PMFSPs) were prepared via host-guest interactions under a facile ultrasonication strategy. By adjusting the stimulation wavelength and utilizing photoinduced fluorescence resonance energy transfer (FRET), the supramolecular polymers can undergo reversible tristate fluorescence switching among none, red, and green. Due to the high contrast, rapid photoresponsiveness and prominent photoreversibility of the prepared PMFSPs, they have great potential in advanced anti-counterfeiting and multilevel information encryption.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Xiang Li, Zhong Lin, Yong Tian, Chonghua Zhang, Peisheng Zhang, Rongjin Zeng, Lei Qiao, Jian Chen
Summary: In this study, a novel negative photochromic spiropyran derivative, MAHD-SP, was designed with stable ring-opened state and notable orange fluorescence. The fluorescence of MAHD-SP was quenched by the Michael addition reaction of SO2 derivative. A ratiometric fluorescent polymeric nanoprobe (RFPN) was constructed using MAHD-SP, a fluorescent conjugated polymer PFO, and a polymeric surfactant PEO113-b-PS49. The RFPN exhibited high sensitivity and selectivity for the ratiometric detection of SO2 derivatives in aqueous solutions, and could visualize SO2 derivative generation in living cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Yong Tian, Yuqi Li, Xiang Li, Zhong Lin, Chonghua Zhang, Qiyun Kuang, Peisheng Zhang, Rongjin Zeng, Jian Chen
Summary: In this study, thermostable photoswitchable red fluorescent polymer nanoparticles (TPFPNs) were developed through a facile miniemulsion polymerization. TPFPNs exhibited adjustable FRET efficiency, outstanding thermal stability, prominent long-term stability, superior photoreversibility, as well as high contrast of on/off fluorescence. Moreover, TPFPNs were successfully used for photo-rewritable patterning and reversible fluorescence bioimaging in Zebrafish.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Jiawei Jiang, Peisheng Zhang, Yong Tian, Zhong Lin, Chonghua Zhang, Jiaxi Cui, Jian Chen, Xudong Chen
Summary: Developing high-performance information encryption materials is essential for advanced technologies that prevent information leakage and combat fraud. The researchers have developed an information encryption toolbox based on self-healing photoswitchable polymers, enabling the production of reconfigurable patterns and codes with excellent mechanical and optical properties.
SCIENCE CHINA-MATERIALS
(2023)
Article
Chemistry, Applied
Tiancheng Wu, Yong Tian, Jingmei Wu, Xiang Li, Ping Wang, Peisheng Zhang, Jiaxi Cui, Jian Chen, Xudong Chen
Summary: Nowadays, high security of information encryption technology, especially those time-dependent encrypting, are increasingly important, but remains a great challenge. Herein, we developed a novel time-dependent information encryption technology, which based on a series of photochromic spirooxazine-contained polymers with different rigidity. The color of these polymers can be reversibly switched between blue and colorless via irradiation of UV light and storage in dark. The decoloration rate of these polymers can be modulated by changing the rigidity of the polymers. By taking full advantage of this property, two self-erased time-dependent information encryption strategies were established. After UV irradiation, the encoded information was self-erased with time in dark. During this process, fake or correct information was generated. The correct information was only identified at a pre-designed period of time. This time-dependent property implies new prospect for information encryption with a high level of security.
Article
Chemistry, Analytical
Ding Zhang, Lei Qiao, Shuaiwei Xu, Lan Peng, Yu Yang, Peisheng Zhang, Zhi-ling Song, Jian Chen, Chong-Hua Zhang
Summary: In this paper, a method based on PIAA-DNAzyme wires was developed for accurate identification of exosomes. The method showed excellent sensitivity and high anti-interference, offering an advantageous platform for precise exosome recognition and early disease detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Xiang Li, Hong Wang, Jian Chen, Yong Tian, Chongchen Xiang, Wanqiang Liu, Zhihua Zhou, Jiaxi Cui, Xudong Chen
Summary: Photoswitchable fluorescent polymers (PFPs) are promising systems for applications such as photowritable pattern, optical anti-counterfeiting, and information encryption. However, the use of harmful UV light as stimulus often leads to photobleaching and poor reversibility. To address this issue, visible-light-driven PFPs are developed by copolymerization of a novel negative photochromic spiropyran monomer and methyl acrylate. These copolymers exhibit bright red fluorescence, which can be reversibly turned off by visible light irradiation. The system demonstrates fast photo-responsibility, prominent photo-reversibility and photostability, simple preparation process, and processability. Applications in photorewritable patterns, optical anti-counterfeiting, and information encryption are demonstrated.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Spectroscopy
Lijie Luo, Jiayuan Cheng, Shu Chen, Peisheng Zhang, Song Chen, Zilong Tang, Rongjin Zeng, Maotian Xu, Yuanqiang Hao
Summary: This study reports a near-infrared ratiometric fluorescent probe (DCPBCl2-Hz) for the detection of hydrazine. The probe has good selectivity, high sensitivity, large Stokes shift, and wide applicable pH range, allowing convenient detection of hydrazine down to 1 ppm. Additionally, the probe can penetrate living cells and visualize intracellular hydrazine.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Haitao Deng, Hong Wang, Yong Tian, Zhong Lin, Jiaxi Cui, Jian Chen
Summary: Photoswitchable supramolecular fluorescent polymers (PSFPs) were prepared by integrating photochromic molecules into a hydrophobic polymer matrix with fluorine elastomer, exhibiting adaptive fluorescence switching between none, green, and red under different light irradiation. PSFPs exhibited excellent photoswitchable properties, outstanding stretchability, hydrophobicity, antifouling, self-healing ability, and simplified preparation process, making them suitable for underwater data encryption and anti-counterfeiting labels applications.
MATERIALS HORIZONS
(2023)
Article
Materials Science, Multidisciplinary
Hong Wang, Jia Tang, Haitao Deng, Yong Tian, Zhong Lin, Jiaxi Cui, Jian Chen
Summary: In this study, photoswitchable fluorescent supramolecular polymers (PFSPs) were prepared through two independent host-guest interactions and successfully applied in information encryption and anti-counterfeiting systems. These polymers demonstrate excellent fluorescence properties, fast photo-responsiveness, and prominent photo-reversibility.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Haitao Deng, Hong Wang, Yong Tian, Zhong Lin, Jiaxi Cui, Jian Chen
Summary: Photoswitchable supramolecular fluorescent polymers (PSFPs) were prepared that can adaptively change their fluorescence between none, green and red by the irradiation of different light. PSFPs exhibited excellent photoswitchable properties and other desired physical properties. They were successfully applied in underwater data encryption and anti-counterfeiting labels.
MATERIALS HORIZONS
(2023)
