Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 410, Issue 18, Pages 4437-4443Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1098-3
Keywords
Halophilic prokaryotes; Carotenoids; Inclusions; Salts; Miniature Raman spectrometer
Funding
- Czech Science Foundation [17-04270S]
- Center for Geosphere Dynamics [UNCE/SCI/006]
- Israel Science Foundation [2221/15]
- Charles University, Prague
- Hebrew University of Jerusalem
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Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4 center dot 7H(2)O, K2SO4, and (NH4)Al(SO4)(2)center dot 12H(2)O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200-4000 cm(-1). This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.
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