Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 410, Issue 7, Pages 2031-2042Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-0882-4
Keywords
Oestrogenic compounds; Magnetic nanoparticles; Micro-dispersive solid-phase extraction; Milk; Ultra-high-performance liquid chromatography; Tandem mass spectrometry
Funding
- Canary Agency of Economy, Industry, Trade and Knowledge of the Government of the Canary Islands - European Social Funds
- Spanish Ministry of Economy and Competitiveness [AGL2011-24667, CTQ2014-57195-P]
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In this work, the suitability of Fe3O4 nanoparticles coated with polydopamine was evaluated as sorbent for the extraction of a group of 21 compounds with oestrogenic activity including seven phytoestrogens, six mycotoxins as well as four synthetic and four natural oestrogens from different types of milk, including sheep milk, in which the evaluation of oestrogenic compounds have never been developed before. Extraction was carried out using magnetic micro-dispersive solid-phase extraction after a previous deproteinisation step. Separation, determination and quantification of the target analytes were achieved by ultra-high-performance liquid chromatography coupled to triple quadrupole-tandem mass spectrometry. The methodology was validated for five milk samples using 17 beta-estradiol-2,4,16,16,17-d(5) as internal standard for natural and synthetic oestrogens, beta-zearalanol-10,10,11,12,12-d(5) for mycotoxins and prunetin for phytoestrogens. Recovery values ranged from 70 to 120% for the five types of matrices with relative standard deviation values lower than 18%. Limits of quantification of the method were in the range 0.55-11.8 mu g L-1 for all samples.
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