Journal
ANALYTICA CHIMICA ACTA
Volume 1025, Issue -, Pages 99-107Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.04.051
Keywords
Urokinase-type plasminogen activator; Biosensor; Photoelectrochemistry; C3N4/CdS
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Funding
- National Natural Science Foundation of China [21471001, 21427807, 21575001]
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Herein, we established a novel ultrasensitive photoelectrochemical biosensor for detecting urokinase-type plasminogen activator (u-PA), based on a g-C3N4/CdS nanocomposite. The prepared nanocomposite was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, ultravioletevisible absorption spectroscopy, and Fourier transform infrared spectroscopy, thus indicating that the nanocomposite was prepared successfully. In the typical process, the prepared nanocomposite was deposited on the surface of a bare FTO electrode. After being air-dried, the g-C3N4/CdS nanocomposite modified electrode was successively incubated with antibody against urokinase-type plasminogen activator and the blocking agent BSA to produce a photoelectrochemical biosensor for u-PA. In the presence of target u-PA antigen, the photocurrent response of the prepared biosensor electrode decreased significantly. The proposed novel photoelectrochemical biosensor exhibited good sensitivity, specificity, and reproducibility for u-PA detection, and a low detection limit of 33 fg mL(-1), ranging from 1 mu g mL(-1)-0.1 pg mL(-1). The proposed strategy should provide a promising method for detection of other biomarkers. (C) 2018 Elsevier B.V. All rights reserved.
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