Journal
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
Volume 315, Issue 4, Pages L545-L552Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00214.2018
Keywords
acute respiratory distress syndrome; auranofin; bronchopulmonary dysplasia; club cells; heme oxygenase-1; nuclear factor E2-related factor 2; thioredoxin reductase
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Funding
- National Heart, Lung, and Blood Institute [R01-HL-119280, R01-HL-119280-S1]
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Thioredoxin reductase-1 (TXNRD1) inhibition effectively activates nuclear factor (erythroid- derived 2)-like 2 (Nrf2) responses and attenuates lung injury in acute respiratory distress syndrome (ARDS) and bronchopulmonary dysplasia (BPD) models. Upon TXNRD1 inhibition, heme oxygenase-1 (HO-1) is disproportionally increased compared with Nrf2 target NADPH quinone oxidoreductase-1 (Nqo1). HO-1 has been investigated as a potential therapeutic target in both ARDS and BPD. TXNRD1 is predominantly expressed in airway epithelial cells; however, the mechanism of HO-1 induction by TXNRD1 inhibitors is unknown. We tested the hypothesis that TXNRD1 inhibition induces HO-1 via Nrf2-dependent mechanisms. Wild-type (WT), Nrf2(KO1.3), and Nrf2(KO2.2) cells were morphologically indistinguishable, indicating that Nrf2 can be deleted from murine-transformed club cells (mtCCs) using CRISPR/Cas9 gene editing. Hemin, a Nrf2-independent HO-1-inducing agent, significantly increased HO-1 expression in WT, Nrf2(KO1.3), and Nrf2(KO2.2). Auranofin (AFN) (0.5 mu M) inhibited TXNRD1 activity by 50% and increased Nqo1 and Hmox1 mRNA levels by 6-and 24-fold, respectively, in WT cells. Despite similar levels of TXNRD1 inhibition, Nqo1 mRNA levels were not different between control and AFN-treated Nrf2(KO1.3) and Nrf2(KO2.2). AFN slightly increased Hmox1 mRNA levels in Nrf2(KO1.3) and Nrf2KO2.2 cells compared with controls. AFN failed to increase HO-1 protein in Nrf2(KO1.3) and Nrf2(KO2.2) compared with a 36-fold increase in WT mtCCs. Our data indicate that Nrf2 is the primary mechanism by which TXNRD1 inhibitors increase HO-1 in lung epithelia. Future studies will use ARDS and BPD models to define the role of HO-1 in attenuation of lung injury by TXNRD1 inhibitors.
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