Article
Chemistry, Multidisciplinary
Yingjie Hang, Xiaoliang Ma, Chunxiao Liu, Siyuan Li, Sixuan Zhang, Ruyan Feng, Qianwen Shang, Qi Liu, Zhaozhao Ding, Xiaoyi Zhang, Liyin Yu, Qiang Lu, Changshun Shao, Hong Chen, Yufang Shi, Jiuyang He, David L. Kaplan
Summary: The development of anisotropic silk protein nanofiber-based hydrogels enables mouse embryonic stem cells to maintain stemness in vitro without the need for leukemia inhibitory factor and mouse embryonic fibroblasts, resulting in superior pluripotency and genetic stability. The hydrogels establish an improved dynamic niche that stimulates autocrine factor secretion and maintains pluripotency and propagation of ESCs, offering a simple and cost-effective option for expansion and utility of ESCs in research and therapy, while providing insights into early mammalian embryogenesis.
Article
Biochemistry & Molecular Biology
Guohua Meng, Andrea Lauria, Mara Maldotti, Francesca Anselmi, Isabelle Laurence Polignano, Stefania Rapelli, Daniela Donna, Salvatore Oliviero
Summary: Smad7 functions as a general transcription factor in ESCs, regulating both TGF-beta unrelated genes and key stemness regulators. Loss of Smad7 results in the downregulation of stemness master regulators and the upregulation of developmental genes, leading to loss of the stem phenotype. Integrative analysis reveals that Smad7 co-occupies promoters of highly expressed key stemness regulators by binding to a specific consensus response element.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Sadegh Ghorbani, Annette Christine Fuchtbauer, Andreas Mollebjerg, Pia Moller Martensen, Sara Hvidbjerg Laursen, David Christian Evar Kraft, Jorgen Kjems, Rikke Louise Meyer, Karim Rahimi, Morten Foss, Ernst-Martin Fuchtbauer, Duncan S. Sutherland
Summary: Biochemical and biomechanical signals in the niche environments regulate stem cell function. A study compared in vitro signals from LN-511 and/or topographic cues to maintain stemness in mESCs. The integration of LN-511 with nanotopography decreased colony growth but maintained stemness markers, suggesting the antagonistic effect of combining biochemical and topographic signals. Faster colony growth was observed at nanotopographies without LN-511, indicating improved ESC expansion.
Article
Biophysics
Jiakai Xing, Jiaxin Jia, Xiaofeng Cong, Ziling Liu, Quanshun Li
Summary: The novel carrier PEN successfully transfected miR-29a into cancer cells, inhibiting cell proliferation and inducing cell cycle arrest, showcasing a potential anti-cancer effect.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Cell Biology
Kimberly J. Berry, Uma Chandran, Fangping Mu, Dinesh K. Deochand, T. Lei, Miriam Pagin, Silvia K. Nicolis, A. Paula Monaghan-Nichols, Inez Rogatsky, Donald B. DeFranco
Summary: Prenatal exposure to synthetic glucocorticoids (sGCs) can reprogram brain development and increase the risk of adverse neurodevelopmental outcomes in the fetus. In a mouse model, researchers found that sGCs caused sexually dimorphic changes in the transcriptome of neural stem and progenitor cells (NSPCs) in the embryonic brain. By analyzing multiple genome-wide assays, they discovered that glucocorticoid receptor (GR) preferentially binds to specific regions of accessible chromatin to influence gene programming and cell fate decisions in NSPCs. They also identified SOX2 as a transcription factor that affects the genomic response of certain GR target genes to sGCs.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Cell Biology
Yuting Fu, Fangyuan Liu, Shuo Cao, Jia Zhang, Huizhi Wang, Baojiang Wu, Yongli Song, Shuguang Duo, Xihe Li, Siqin Bao
Summary: This study reveals that Bdh2 deficiency has no impact on pluripotency and self-renewal of ESCs, but promotes endoderm differentiation and is essential for maintaining DNA methylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Shi-Meng Guo, Yi-Ran Zhang, Bing-Xin Ma, Li-Quan Zhou, Ying Yin
Summary: In this study, the inhibition of DRP1 activity in mESCs led to an increase in cleavage embryo gene levels and a tendency towards a new expression profile similar to the 2C-like state. Additionally, the glycolysis/gluconeogenesis pathway and its related enzymes were downregulated in 2C-like cells. Furthermore, inhibiting DRP1 activity from the late zygote stage resulted in inhibited development of early embryos and inefficient silencing of cleavage embryo genes at the late 2C stage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Oliver Brookes, Stephen D. Thorpe, Olga Rigby Evans, Michael C. Keeling, David A. Lee
Summary: This study investigated the relationship between biomechanical properties and pluripotency gene expression in murine embryonic stem cells, revealing considerable variation among cells, with high pluripotency gene expression correlating to lower cell stiffness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Anderson Tan, Renuka Prasad, Eek-hoon Jho
Summary: TFEB plays a crucial role in mESCs by regulating PTN to maintain pluripotency, with interactions involving Nanog and Sox2. While high levels of TFEB are expected to enhance autophagy-lysosomal activity, undifferentiated mESCs surprisingly display low basal levels.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Rong Mu Xia, Dong Bo Yao, Xue Min Cai, Xiu Qin Xu
Summary: This study elucidated the regulatory role of histidine phosphatase LHPP in ESCs self-renewal through histidine dephosphorylation. LHPP negatively regulated the Wnt/β-catenin pathway by modulating histidine dephosphorylation, thereby inhibiting the self-renewal of ESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Raghu Ramanathan, Nathan A. Delvaux, Kevin G. Rice
Summary: We developed an improved in vitro transfection assay for testing the efficiency of non-viral vector DNA nanoparticle transfection of primary hepatocytes. The assay was optimized for cell plating number, DNA dose, and PEI to DNA ratio, and provides reliable in vitro expression results for direct comparison of different non-viral gene delivery vectors.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Developmental Biology
Dhruv Raina, Fiorella Fabris, Luis G. Morelli, Christian Schroter
Summary: Signal transduction networks play important roles in guiding cell fate decisions, and the differentiation of pluripotent cells is controlled by FGF/ERK signaling. This study reveals that dynamic ERK activity in mouse embryonic stem cells exhibits pulsing patterns, which are heterogeneous between cells. The prevalence of pulse sequences is influenced by ligand concentration and cell differentiation state, suggesting that FGF/ERK signaling operates in a transition point between oscillatory and non-oscillatory dynamics.
Article
Biochemistry & Molecular Biology
Martin Hsiu-Chu Lin, Ping-Shan Lai, Li-Ching Chang, Wei-Chao Huang, Ming-Hsueh Lee, Kuo-Tai Chen, Chiu-Yen Chung, Jen-Tsung Yang
Summary: The study combines PS80 PBCA NP with chitosan surface modification for the transfection-guided neural-lineage specification of mouse iPSCs. The complex exhibits high stability, low cell toxicity, and enhances BDNF expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Medicine, General & Internal
Colleen M. Bartman, Yoshimasa Oyama, Tobias Eckle
Article
Pharmacology & Pharmacy
Yoshimasa Oyama, Colleen Marie Bartman, Jennifer Gile, Daniel Sehrt, Tobias Eckle
CURRENT PHARMACEUTICAL DESIGN
(2018)
Review
Pharmacology & Pharmacy
Colleen Marie Bartman, Tobias Eekle
CURRENT PHARMACEUTICAL DESIGN
(2019)
Article
Cell Biology
Yoshimasa Oyama, Colleen M. Bartman, Stephanie Bonney, J. Scott Lee, Lori A. Walker, Jun Han, Christoph H. Borchers, Peter M. Buttrick, Carol M. Aherne, Nathan Clendenen, Sean P. Colgan, Tobias Eckle
Review
Medicine, Research & Experimental
Colleen M. Bartman, Aleksey Matveyenko, Y. S. Prakash
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Editorial Material
Physiology
Colleen M. Bartman, Y. S. Prakash
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Colleen M. Bartman, Marta Schiliro, Martin Helan, Y. S. Prakash, David Linden, Christina Pabelick
Review
Respiratory System
Marta Schiliro, Colleen M. Bartman, Christina Pabelick
Summary: H2S has anti-inflammatory, antioxidant, and antiapoptotic properties at physiological concentrations; both endogenous and exogenous H2S have protective effects on the respiratory system; H2S shows potential as a novel candidate for protecting lung function following preterm birth.
EXPERT REVIEW OF RESPIRATORY MEDICINE
(2021)
Article
Physiology
Colleen M. Bartman, Aleksey Matveyenko, Christina Pabelick, Y. S. Prakash
Summary: This study explores the impact of hyperoxia on the clock function in human fetal airway smooth muscle cells and how manipulating clock genes can alleviate the deleterious effects of oxygen on developing airways. Findings suggest that manipulating clock proteins can modulate intracellular calcium regulation in fASM, particularly under hyperoxia, offering a potential new therapeutic avenue for neonatal airway diseases following prematurity.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Physiology
Anne M. Roesler, Jovanka Ravix, Colleen M. Bartman, Brijeshkumar S. Patel, Marta Schiliro, Benjamin Roos, Lisa Nesbitt, Christina M. Pabelick, Richard J. Martin, Peter M. MacFarlane, Y. S. Prakash
Summary: Study found that high O2 affected CaSR expression in fetal lung ASM cells, leading to increased cellular calcium responses and stimulated intracellular and extracellular calcium ion responses, which have important implications for respiratory diseases such as asthma.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Physiology
Colleen M. Bartman, Kimberly E. Stelzig, David R. Linden, Y. S. Prakash, Sergio E. Chiarella
Summary: The study presents a simple and effective method for siRNA transfection of normal HBEs in ALI cultures, without the need for potentially cytotoxic transfection reagents and without detrimental effects on the physiology or morphology of NHBEs. This transfection protocol introduces a reproducible and efficient method for loss-of-function studies in HBE ALI cultures that can be leveraged for modeling the respiratory system and airway diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Colleen M. Bartman, Daniel Wasim Awari, Christina M. Pabelick, Y. S. Prakash
Summary: This study investigates the impact of oxygen on mitochondria in human fetal airway smooth muscle cells exposed to different oxygen levels. The findings suggest that oxygen changes significantly affect mitochondrial structure and function in developing airways, highlighting the importance of balancing oxidative damage and antioxidant defense systems.
Article
Physiology
Ashley Y. Gao, Ana M. Diaz Espinosa, Fiorenza Giani, Tho X. Pham, Chase M. Carver, Aja Aravamudhan, Colleen M. Bartman, Giovanni Ligresti, Nunzia Caporarello, Marissa J. Schafer, Andrew J. Haak
Summary: Cellular senescence is closely associated with idiopathic pulmonary fibrosis (IPF), and Pim-1 kinase has been identified as a driver of NF-kappa B activity, enhancing the production of inflammatory cytokines in IPF fibroblasts. Inhibition of Pim-1 kinase can significantly reduce the expression of cytokines and chemokines in IPF fibroblasts. These findings suggest the therapeutic potential of targeting Pim-1 kinase to reprogram the secretome of senescent fibroblasts and halt IPF progression.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Review
Medicine, General & Internal
Emily Y. Y. Zhang, Colleen M. Bartman, Y. S. Prakash, Christina M. Pabelick, Elizabeth R. Vogel
Summary: Chronic airway diseases, such as wheezing and asthma, are significant sources of morbidity and mortality in children, particularly in preterm infants. Perinatal risk factors, such as respiratory support and mechanical ventilation, may contribute to the development of chronic airway disease. Lower levels of oxygen and stretch exposure due to mechanical ventilation or CPAP may also play a role in this development. This review summarizes the current knowledge on the impact of perinatal oxygen and mechanical respiratory support on the development of chronic pediatric lung disease, with a focus on pediatric airway disease, and explores potential mechanisms for novel therapies.
FRONTIERS IN MEDICINE
(2023)
Article
Physiology
Niyati A. Borkar, Michael A. Thompson, Colleen M. Bartman, Venkatachalem Sathish, Y. S. Prakash, Christina M. Pabelick
Summary: Exposure to nicotine from cigarette smoke and e-cigarettes can increase health risks associated with asthma by affecting airway dynamics. Nicotine disrupts mitochondrial morphology and balance between fusion and fission, impairs mitochondrial calcium regulation, and alters mitochondrial respiratory and bioenergetic functions in airway smooth muscle cells. These effects are mediated through the alpha 7 subtype of the nicotinic acetylcholine receptor.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
