Review
Biotechnology & Applied Microbiology
Titouan Montheil, Matthieu Simon, Daniele Noel, Ahmad Mehdi, Gilles Subra, Cecile Echalier
Summary: Natural biopolymers are widely used in bioinks, but covalent chemical hydrogels are needed to improve mechanical properties and biological functionality. However, there have been limited efforts in developing new chemical reactions that meet the requirements of cell encapsulation and chemical orthogonality. Sol-gel inorganic polymerization offers an alternative crosslinking reaction for hydrogel preparation and the silylation of biomolecules has been explored. This review discusses the sol-gel process in biocompatible conditions and the use of silylated molecules as bioink components.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hepi H. Susapto, Dana Alhattab, Sherin Abdelrahman, Zainab Khan, Salwa Alshehri, Kowther Kahin, Rui Ge, Manola Moretti, Abdul-Hamid Emwas, Charlotte A. E. Hauser
Summary: By utilizing rationally designed ultrashort peptide bioinks, this study overcomes limitations in current bioprinting procedures and demonstrates the potential for automated complex tissue fabrication with stable large-scale constructs and long-term cell survival.
Article
Polymer Science
Emine Karakaya, Faina Bider, Andreas Frank, Joerg Tessmar, Lisa Schoebel, Leonard Forster, Stefan Schruefer, Hans-Werner Schmidt, Dirk Wolfram Schubert, Andreas Blaeser, Aldo R. Boccaccini, Rainer Detsch
Summary: In the context of bioprinting, targeted printing of cells at specific positions is a novel approach that offers new possibilities for life science engineering. However, achieving high cell survival rates on gel substrates remains a challenge. This study evaluates the use of alginate-dialdehyde-polyethylene glycol (ADA-PEG) inks for drop on demand (DoD) printing, analyzing their biochemical properties, printing process, cell distribution, and shear forces. The findings highlight the importance of PEG modifications and chain lengths in achieving stable hydrogels and biocompatibility, as well as the need to determine the optimal cell concentration for targeted cell printing.
Article
Engineering, Biomedical
Juhi Chakraborty, Xuan Mu, Ankita Pramanick, David L. Kaplan, Sourabh Ghosh
Summary: 3D printing has shown rapid growth in the field of regenerative medicine and tissue engineering for bioprinting. Silk Fibroin (SF) as a bioink exhibits exceptional features and versatility for printing complex structures with tunable mechanical properties, degradation rates, and cytocompatibility. This article summarizes recent advances and needs in using SF protein as a bioink, including crosslinking methods for extrusion bioprinting and maintaining cell viability during and post bioprinting. It also discusses the differentiation of encapsulated cells within SF-based 3D bioprinted constructs and explores the applications of SF-based 3D printing, such as magnetically decorated hydrogels and in situ bioprinting. Future perspectives on improving printing strategies and utilizing multicomponent bioinks for improved print fidelity are also explored.
Review
Engineering, Biomedical
Susmita Ghosh, Hee-Gyeong Yi
Summary: Characterization and fabrication methods of new bioinks have gained attention in recent years, as the absence of bioprintable materials is a fundamental challenge for rapid progress in 3D printing. While bioinks for printing mammalian organs have been developed, there is still a lack of research in the field of plant science. This review article highlights the significance of bioinks and their applications in plant bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Biochemistry & Molecular Biology
Kelsey Willson, Anthony Atala, James J. Yoo
Summary: Developing appropriate bioinks is a complex task that depends on the mechanical and biochemical requirements of the final construct and the type of printer used. Different types of tissue printers have different requirements for bioinks, with an emphasis on supporting cellular development. Researchers are increasingly using composite bioinks to take advantage of multiple materials in the development process.
Article
Engineering, Biomedical
Ke Zhou, Rongjian Ding, Xiwang Tao, Yuwen Cui, Jiquan Yang, Hongli Mao, Zhongwei Gu
Summary: Although 3D bioprinting has emerged as an advanced method for fabricating complex in vitro models, developing suitable bioinks that fulfill the requirements for the biofabrication window is still challenging. In this study, a peptide-dendrimer-reinforced bioink is introduced, which allows for the bioprinting of complex structures and exhibits improved mechanical and rheological properties.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
X. B. Chen, A. Fazel Anvari-Yazdi, X. Duan, A. Zimmerling, R. Gharraei, N. K. Sharma, S. Sweilem, L. Ning
Summary: Bioinks combine biomaterials and living cells, and are used in extrusion bioprinting to create 3D structures that resemble human tissue or organs. These printed constructs have various applications in tissue engineering and in vitro tissue modeling. The properties of the bioinks, including rheological, mechanical, and biological properties, as well as the printing process, play a crucial role in successful printing and application. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, emphasizing bioink synthesis, characterization, and their influence on the printing process.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Manuel G. Ricardo, Peter H. Seeberger
Summary: Biomaterials with improved biological features can be obtained by conjugating glycans to nanostructured peptides. Peptide-glycan chimeras were successfully obtained through the merging of peptide and glycan solid-phase syntheses using a bifunctional monosaccharide. Different chimeras containing various sugars and peptides were prepared and exhibited different self-assembling properties.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Manuel G. Ricardo, Peter H. Seeberger
Summary: Biomaterials with improved biological features can be obtained by conjugating glycans to nanostructured peptides. Creating peptide-glycan chimeras requires superb chemoselectivity. We expedite access to such chimeras by merging peptide and glycan solid-phase syntheses employing a bifunctional monosaccharide.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Engineering, Biomedical
Yanli Cai, Soon Yee Chang, Soo Wah Gan, Sha Ma, Wen Feng Lu, Ching-Chiuan Yen
Summary: This review paper focuses on the recent developments in cell-laden nanocomposite bioinks for 3D bioprinting and their effects on the properties and performance of the bioinks. The reinforcement mechanisms and interactions between cells and nano-materials are discussed, with an emphasis on bone and cartilage tissue engineering applications. The limitations and future prospects of current cell-laden nanocomposite bioinks are also addressed.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Sarah M. Hull, Christopher D. Lindsay, Lucia G. Brunel, Daniel J. Shiwarski, Joshua W. Tashman, Julien G. Roth, David Myung, Adam W. Feinberg, Sarah C. Heilshorn
Summary: UNION bioinks represent a versatile and universal strategy for 3D bioprinting, allowing for the printing of different cell types while maintaining high cell viability and characteristic phenotypic markers. This approach expands the toolkit of customizable materials available for 3D bioprinting.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Chixuan Liu, Yen Wah Tong
Summary: Bioprinting is a precise manufacturing technology for customized 3D biological constructs utilizing biomaterials, cells, and sometimes growth factors. However, its translational application is hindered by the lack of efficient techniques for blood vessel fabrications. In this report, an efficient blood vessel bioprinting technique based on interfacial polyelectrolyte complexation was proposed and investigated. This technique demonstrated clear vascular features and the study on peptide sequencing's effect on the biocompatibility of the polyelectrolyte-peptide amphiphile complex is also interesting and relevant for vascular structure fabrication research.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Johannes Windisch, Olena Reinhardt, Sarah Duin, Kathleen Schuetz, Nuria Juliana Novoa Rodriguez, Suihong Liu, Anja Lode, Michael Gelinsky
Summary: Bioprinting, a key technology for future space missions, is being established on the International Space Station (ISS). Researchers have investigated the storability of bioinks, aiming to produce bioinks on Earth and transport them to the ISS. The study shows that bioinks containing microalgae or human cells can be stored at 4 degrees C for four weeks without losing printability, but the viability of human cells decreases during storage. This research provides a promising starting point for future studies on storing bioinks and improving complex printing processes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Engineering, Biomedical
Guy Decante, Joao B. Costa, Joana Silva-Correia, Maurice N. Collins, Rui L. Reis, J. Miguel Oliveira
Summary: Three-dimensional (3D) bioprinting technology has gained significant attention in biomedical engineering and clinical applications, with advancements in bioink development allowing for more precise mimicry of native tissue characteristics and support for biofunctionality.
Article
Biochemistry & Molecular Biology
Anna Majkowska, Karla E. Inostroza-Brito, Mariel Gonzalez, Carlos Redondo-Gomez, Alistair Rice, Jose Carlos Rodriguez-Cabello, Armando E. Del Rio Hernandez, Alvaro Mata
Summary: The study demonstrates the possibility to generate coassembling tubular membranes with distinct structures, properties, and bioactivity by minor modifications in the molecular structures of peptide amphiphiles and elastin-like recombinamers.
Article
Engineering, Biomedical
Hui Ying Lai, Magdiel Inggrid Setyawati, Catarina Vizetto Duarte, Huei Min Chua, Choon Teck Low, Kee Woei Ng
Summary: Human hair proteins, especially keratins, are found to have high radical scavenging activity and can protect human dermal fibroblasts from oxidative stress.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Mechanics
C. R. Constante-Amores, T. Abadie, L. Kahouadji, S. Shin, J. Chergui, D. Juric, A. A. Castrejon-Pita, O. K. Matar
Summary: This study investigates the effect of insoluble surfactants on the spatio-temporal evolution of turbulent jets through three-dimensional numerical simulations. The results show that surfactant-induced Marangoni stresses play a crucial role in the formation of coherent vortex structures and greatly influence the development of three-dimensional interfacial dynamics. The study also provides theoretical expressions for the mechanisms influencing the circulation production rate in the presence of surfactants.
JOURNAL OF FLUID MECHANICS
(2023)
Article
Mechanics
C. R. Constante-Amores, L. Kahouadji, S. Shin, J. Chergui, D. Juric, J. R. Castrejon-Pita, O. K. Matar, A. A. Castrejon-Pita
Summary: We investigate the influence of insoluble surfactants on the impact of surfactant-free droplets onto surfactant-laden thin liquid films using a three-dimensional numerical simulation approach. Our results show that the presence of surfactants affects the late stages of the dynamics by delaying the end-pinching mechanisms, resulting in longer ligaments. We also demonstrate that the addition of surfactants leads to surface rigidification and retards the flow dynamics.
JOURNAL OF FLUID MECHANICS
(2023)
Review
Cell Biology
Sunil S. Adav, Kee Woei Ng
Summary: Aging is a complex process that affects physiological functions, including hair graying, thinning, and loss. These changes in hair can have a significant impact on an individual's self-esteem and mental health. Omics technologies have helped lead to the discovery of hair aging biomarkers and potential therapies.
AGEING RESEARCH REVIEWS
(2023)
Article
Engineering, Environmental
Samraj Mollick, Yang Zhang, Waqas Kamal, Michele Tricarico, Annika F. Moslein, Vishal Kachwal, Nader Amin, Alfonso A. Castrejon-Pita, Stephen M. Morris, Jin-Chong Tan
Summary: By utilizing the concept of nanoconfinement and optimal surface design, organic solid-state photochromic materials with excellent mechanical robustness and photoefficiency have been developed. Commercial prototypes, such as smart windows and photochromic sculptures, have been achieved through blending with polymers.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Genetics & Heredity
Sunil S. Adav, Ching Yung Leung, Kee Woei Ng
Summary: This study utilized proteomic analysis to profile hair characteristics of individuals from different ethnic groups, genders, and ages, and found that hair proteins can be used for individual identification and differentiation.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2023)
Article
Biochemical Research Methods
Junseob Kim, Sunghwan Cho, Justin P. Coon, Alfonso A. Castrejon-Pita, Hamidreza Arjmandi
Summary: This paper proposes a novel electrophoretic molecular communication (EMC) framework utilizing a piecewise constant electric field. The framework exploits electric fields to enhance communication performance by inducing the movement of charged particles. Two optimization problems are formulated to design the electric field based on different objectives: minimizing a mean squared error and minimizing a bit interval. The proposed piecewise constant electric field significantly reduces the bit error rate compared to the constant field benchmark while performing slightly worse than the exponential field benchmark.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2023)
Article
Biophysics
Radu Cimpeanu, Alfonso A. Castrejon-Pita, Lee Nai Lim, Manu Vatish, Ektoras X. Georgiou
Summary: This article introduces a novel hydrodynamics-based needle design that generates a flow field within the follicle to successfully retrieve oocytes without added stress. Through numerical simulations and testing, it is shown that this new design has a higher success rate compared to traditional designs, and the oocytes experience minimal stress.
JOURNAL OF BIOMECHANICS
(2023)
Article
Mechanics
Thomas C. Sykes, Radu Cimpeanu, Ben D. Fudge, J. Rafael Castrejon-Pita, Alfonso A. Castrejon-Pita
Summary: When a fast droplet impacts a pool of the same fluid, the depth of the pool is revealed to be a critical factor in the formation of ejecta sheets. On deeper pools, the sheets fold inwards, while on shallower pools, they develop into outward-propagating lamellae. This transition is caused by a confinement effect of the pool base on the impact-induced pressure, which restricts flow into the ejecta sheet from the droplet.
JOURNAL OF FLUID MECHANICS
(2023)
Article
Materials Science, Multidisciplinary
Waqas Kamal, Alva C. J. Orr, Thomas C. Sykes, Alfonso A. Castrejon-Pita, Steve J. Elston, Stephen M. Morris
Summary: In this study, researchers demonstrated the ability to tune the pitch and reflection band by depositing picolitre volumes of nematic liquid crystal into printed chiral nematic liquid crystal droplets. The redshift of the reflection band can be controlled by varying the number of droplets deposited. This method showcases the potential for creating colored images using this pitch tuning process.
MATERIALS TODAY ADVANCES
(2023)
Article
Materials Science, Multidisciplinary
Samyukta Ravishankar, Anu Maashaa Nedumaran, Archana Gautam, Kee Woei Ng, Bertrand Czarny, Sierin Lim
Summary: Nanoparticles, both organic and inorganic, are used as tools for diagnostic and therapeutic applications. However, there is limited understanding of the cellular fate and molecular responses of organic nanoparticles in macrophages. In this study, we investigated the intracellular fate and immune responses of a model organic nanoparticle, Archaeoglobus fulgidus ferritin, in murine macrophage cells.
NPG ASIA MATERIALS
(2023)
Article
Engineering, Environmental
Melissa Kao Hui Lee, Hong Kit Lim, Chengxun Su, Jie Yan Cheryl Koh, Magdiel Inggrid Setyawati, Kee Woei Ng, Han Wei Hou, Chor Yong Tay
Summary: This study used a three-dimensional biomimetic microfluidic lung-on-chip platform and RNA sequencing to investigate the effects of two model nanoparticles on airway epithelium cells. It was found that even low-level exposure to these nanoparticles triggered chemotaxis of lung fibroblasts and induced a transcriptomic response associated with the development of lung fibrosis. These findings provide new insights into the early acute events of respiratory harm caused by environmental nanoparticles exposure.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)