Journal
ACTA DERMATO-VENEREOLOGICA
Volume 98, Issue 1, Pages 44-49Publisher
ACTA DERMATO-VENEREOLOGICA
DOI: 10.2340/00015555-2738
Keywords
melanoma; BRAF; NRAS; immunohistochemistry; Idylla; next-generation sequencing
Categories
Funding
- Omnium Group
Ask authors/readers for more resources
BRAF and NRAS genetic analyses are time-consuming and can delay treatment choices in patients with metastatic melanomas presenting with acute deterioration. We compared the rapid, real-time, fully automated molecular diagnosis platform Idylla (TM) with next-generation sequencing (NGS) and immunohistochemistry for detection of BRAF and NRAS mutations in 36 patients with metastatic melanomas. The Idylla (TM) NRAS-BRAF-EGFRS492R mutation assay (110 min per sample) detected BRAF and NRAS mutations in 15 and 17 samples, respectively. One NRAS mutation was different between NGS and Idylla (TM) (NRASG13C vs. NRASG12A/D). Four samples were BRAF and NRAS wild-type. The global concordance between NGS and Idylla (TM) assays was 97.2% (35/36 cases). Immunohistochemistry was positive only in 9/9 BRAFV600E and 6/6 NRASQ61R-mutated samples with VE1 and SP174 antibodies, respectively. The Idylla (TM) platform is a valuable rapid molecular diagnosis tool to reduce the delay in BRAF and NRAS analyses-related treatment choices for patients with metastatic melanoma presenting with acute deterioration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available