Article
Polymer Science
Rikako Hama, Derya Aytemiz, Kelvin O. Moseti, Tsunenori Kameda, Yasumoto Nakazawa
Summary: Silk fibroin (SF) has great potential as a biomaterial due to its shape and structure. This study successfully modified SF with heparin using tyrosine residues as reaction points, which improved its ability to bind to high-molecular-weight and hydrophilic compounds. Increased heparin modification led to higher heparin content and improved water wettability on film surfaces, promoting the proliferation of endothelial cells and fibroblasts.
Article
Polymer Science
Tanrada Fungmongkonsatean, Jirapas Jongjitwimol, Pussadee Paensuwan, Teonchit Nuamchit, Duangduan Siriwittayawan, Sorada Kanokpanont, Siriporn Damrongsakkul, Piyanuch Thitiwuthikiat
Summary: The hemocompatibility of Thai Bombyx mori silk fibroin (SF) was investigated and improved by immobilizing low molecular weight heparin (LMWH). The endothelial cell proliferation on LMWH-immobilized SF samples was evaluated. The hemocompatibility evaluation showed that Thai SF did not have adverse effects on clotting time, complement and leukocyte activation, and was non-hemolytic compared to the negative control. LMWH immobilization improved the surface hydrophilicity of SF films and demonstrated slightly improved hemocompatibility.
Article
Engineering, Environmental
Do Kyung Kim, Sanghyuk Lee, Minjeong Kim, Yunhui Jeong, Soonchul Lee
Summary: The study demonstrates that using MSC-derived exosomes coated SF scaffolds can effectively promote bone regeneration. In vitro assay showed positive effects of the scaffolds on the growth and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells, while in vivo study confirmed the thorough integration and attachment of implanted scaffolds in the bone defect region.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Polymer Science
Huiyan Shao, Ziling Sun
Summary: Silk fibroin (SF) is commonly used in different applications due to its biocompatibility, controllable biodegradability, minimal inflammation, and adjustable mechanical properties. Neovascularization of SF is essential for successful wound healing, as it provides nutrient supply to the granulation tissue and promotes vessel formation and remodeling. This review summarizes the neovascularization of SF in various forms and dissolutions through the use of different composites, modified active peptides, and added growth factors in the treatment of different wound healing processes.
Review
Biochemistry & Molecular Biology
Weizhen Sun, David Alexander Gregory, Mhd Anas Tomeh, Xiubo Zhao
Summary: Tissue engineering involves combining cells, scaffold materials, and growth factors to regenerate or replace damaged tissues or organs. Silk fibroin has attracted attention for its outstanding properties and ability to be reconstructed into various material formats for tissue engineering applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Guangfei Li, Shan Sun
Summary: Silk fibroin scaffolds are widely used materials in tissue engineering, with excellent properties and diverse forms that can be achieved through various fabrication techniques. These scaffolds play a significant role in various tissue engineering applications.
Article
Green & Sustainable Science & Technology
Chunhong Yang, Songmin Shang, Dahua Shou, Luoxiao Ran, Guangqian Lan, Enling Hu
Summary: This study focuses on upcycling disqualified silk fibers by transforming natural silk nonwovens into bioadhesives for tissue repair. The technique involves pretreating the silk nonwovens to obtain a modified cocoon sheet (MCS) and surface modification to achieve controllable adhesion. The resulting bioadhesive showed superior performance in repairing tissues and has the potential to bridge the gap between advanced biomaterials and disqualified silk wastes.
JOURNAL OF CLEANER PRODUCTION
(2021)
Review
Engineering, Biomedical
Prerak Gupta, Biman B. Mandal
Summary: Vascular tissue engineering is focusing on finding biologically active substitutes with in situ growth potential, with silk biomaterials showing remarkable potential due to their favorable biological and mechanical properties.
ACTA BIOMATERIALIA
(2021)
Review
Medicine, Research & Experimental
Ziyang Zhou, Jin Cui, Shunli Wu, Zhen Geng, Jiacan Su
Summary: Osteoarthritis is a common joint disease that causes physical and psychological harm to patients and burdens the healthcare system. Tissue engineering has been focusing on cartilage repair, with silk fibroin as a promising biomaterial due to its unique properties and capabilities for functional modification.
Review
Biochemistry & Molecular Biology
Minghui Li, Jiaqian You, Qiuyue Qin, Manxuan Liu, Yixin Yang, Kewen Jia, Yidi Zhang, Yanmin Zhou
Summary: Bone tissue engineering (BTE) utilizes silk fibroin (SF) to regulate bone regeneration and stimulate bone formation. SF is a promising material due to its tunable mechanical properties, biodegradability, and biocompatibility. This review introduces the structure and characteristics of SF, summarizes its immunological mechanism for osteogenesis, and emphasizes the various forms of SF biomaterials and their development prospects in BTE. Biomaterials based on SF have great potential in bone tissue engineering, and this review guides future design and research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Shihui Jiang, Zhaoxia Yu, Lanrui Zhang, Guanhua Wang, Xiaohua Dai, Xiaoli Lian, Yan Yan, Linpu Zhang, Yue Wang, Ruixin Li, Huiru Zou
Summary: This study evaluated the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on human dental pulp cells (HDPCs) proliferation and differentiation. The scaffolds improved cell adhesion and proliferation, as well as significantly increased ALP activity, suggesting their potential as candidates for dentine-pulp complex regeneration.
REGENERATIVE BIOMATERIALS
(2021)
Review
Chemistry, Physical
Federica Paladini, Mauro Pollini
Summary: Bone tissue engineering is a multidisciplinary research field that aims to create biological substitutes for bone regeneration. The selection of appropriate biomaterials and structures remains a challenge, and silk fibroin is considered a valuable option due to its unique biological properties and processability.
Article
Biochemistry & Molecular Biology
Natasha Bokhari, Asif Ali, Abida Yasmeen, Hamad Khalid, Sher Zaman Safi, Faiza Sharif
Summary: Soft tissue defects can be resolved with coated silk meshes, which have antimicrobial and wound healing properties. The coating alters the pore size and fiber diameter of the mesh, while also improving its mechanical strength. Among the variants tested, MHRJ showed the best cell viability and wound healing effects. In vivo experiments with rat hernia model confirmed the non-toxic nature and wound healing characteristics of the modified mesh. This study suggests that MHRJ could be a valuable biomedical implant.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Francisco Vieira dos Santos, Renato Luiz Siqueira, Lucas de Morais Ramos, Sergio Akinobu Yoshioka, Marcia Cristina Branciforti, Daniel Souza Correa
Summary: This review highlights the versatile application of silk fibroin (SF)-derived electrospun materials in tissue regeneration, disease treatment, and drug delivery systems, as well as explores potential future trends and limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Promita Bhattacharjee, Mark Ahearne
Summary: There is a need to develop tissue engineering methods to address the shortage of donor corneas for transplantation. A novel approach using free-radical polymerization to fabricate three-dimensional hydrogels for corneal stromal tissue regeneration was investigated. The hydrogels showed rapid gelation, good porosity, enhanced cellular adhesion and proliferation, and no cytotoxicity. This study provides an initial step towards developing clinically applicable ocular implants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Engineering, Biomedical
Pingping Han, Guillermo A. Gomez, Georg N. Duda, Saso Ivanovski, Patrina S. P. Poh
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Ranieri Cancedda, J. Mary Murphy, Martijn Van Griensven
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Claudia Del Toro Runzer, Shivesh Anand, Carlos Mota, Lorenzo Moroni, Christian Plank, Martijn van Griensven, Elizabeth R. Balmayor
Summary: Nucleic acids have great potential for gene therapy, with plasmid DNA as the first therapeutic nucleic acid and mRNA as a safer and more affordable option. This study investigated the uptake mechanisms and efficiencies of genetic material by cells, focusing on different nucleic acids, delivery vectors, and human primary cells. The study found that caveolae-mediated uptake was the main route for gene delivery, pDNA had higher expression levels in fast-dividing fibroblasts, cmRNA was responsible for high protein production in slow-dividing osteoblasts, and in mesenchymal stem cells, the combination of vector and nucleic acid was more relevant. Protein expression was higher when cells were seeded on 3D scaffolds.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biotechnology & Applied Microbiology
Eduardo Gushiken, Milagros Quintana, Luz Agueda Perez, Patricia Herrera-Velit, Elizabeth R. Balmayor, Martijn van Griensven, Jose R. Espinoza
Summary: This study successfully isolated ASCs using anti-CD105 VHH conjugated magnetic beads, which showed high selectivity and recovery rate. This method may provide a new strategy for MSCs isolation.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Emergency Medicine
Rald V. M. Groven, Job T. Blokhuis, Martijn Poeze, Martijn van Griensven, Taco J. Blokhuis
Summary: Recently, a surgical suction filter device was introduced to generate a suction filter-derived bone grafting substitute (SF-BGS). This study aimed to investigate the quality of the SF-BGS by determining the expression of miRNAs and osteogenic mRNAs. The results showed distinct differences in miRNA expression and overall upregulation of osteogenic marker genes in SF-BGS.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Carlos Julio Peniche Silva, Rodolfo E. de la Vega, Joseph Panos, Virginie Joris, Christopher H. Evans, Elizabeth R. Balmayor, Martijn van Griensven
Summary: In this study, the miRNA expression profile in enthesis tissue following injury was investigated. Target prediction and mRNA expression analysis suggest that certain miRNAs may regulate the expression of mRNA targets relevant for enthesis healing. Additionally, the protein expression levels of collagens I, II, III, and X were found to change after injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Immunology
Edwin Boelke, Martijn van Griensven, E. Marion Schneider, Johannes C. Fischer, Torsten Feldt, Verena Keitel, Wilfried Budach, Jan Haussmann, Danny Jazmati, Christiane Matuschek
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Rald Victor Maria Groven, Johannes Greven, Umit Mert, Klemens Horst, Qun Zhao, Taco Johan Blokhuis, Markus Huber-Lang, Frank Hildebrand, Martijn van Griensven
Summary: This study investigates the effects of Early Total Care (ETC) and Damage Control Orthopedics (DCO) on microRNA (miRNA) expression in extracellular vesicles (EVs) and identifies their mRNA targets. The results show that the miRNA expression patterns in the ETC group are more pro-inflammatory, indicating potentially decreased tissue regeneration capabilities. In silico target prediction reveals mRNA targets related to inflammation, fibrosis, and Wnt signaling. Validation of these targets in lung tissue shows treatment-specific deregulations that match the expression of their upstream miRNAs, while no significant deregulations were observed in the liver.
FRONTIERS IN IMMUNOLOGY
(2023)
Editorial Material
Medicine, Research & Experimental
Elizabeth R. Balmayor
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Materials Science, Multidisciplinary
Pichaporn Sutthavas, Matthias Schumacher, Martyna Nikody, Vaijayanthi Ramesh, Jurij Jakobi, Elizabeth R. R. Balmayor, Pamela Habibovic, Christoph Rehbock, Stephan Barcikowski, Sabine van Rijt
Summary: Bioactive glass nanoparticles (nBGs) have been successfully doped with iron, strontium, and/or copper ions using pulsed laser techniques. The laser-doped nBGs maintained their morphology and showed biocompatibility. Furthermore, the presence of specific ions in the nBGs enhanced bone repair and vessel formation.
MATERIALS ADVANCES
(2023)
Article
Medicine, Research & Experimental
Danny Jazmati, Balint Tamaskovics, Norman-Philipp Hoff, Bernhard Homey, Edwin Boelke, Belebenie Boyomo, Waseem Garabet, Jan Haussmann, Wilfried Budach, Judith Neuwahl, Hubert Schelzig, Stefanie Corradini, Martijn van Griensven, Johannes Fischer, Wolfram Trudo Knoefel, John Pegani, Alessia Pedoto, Gerald Antoch, Julian Kirchner, Tom Luedde, Noemi F. Freise, Torsten Feldt, Bjoern-Erik Ole Jensen, Verena Keitel, Christiane Matuschek
Summary: Low-dose irradiation is an effective treatment option for persistent lymphatic fistulas after inguinal vascular surgery, preventing infection, sepsis, bleeding, and possible leg amputation. The recommended dose ranges from 0.3-0.5 to 1-12 Gy. In this study, every patient experienced resolution of the lymphatic fistula without major complications.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Immunology
Bingduo Wang, Aliona Woehler, Johannes Greven, Rebekka J. S. Salzmann, Cindy M. Keller, Tobias Tertel, Qun Zhao, Uemit Mert, Klemens Horst, Ludmila Lupu, Markus Huber-Lang, Martijn van Griensven, Tom Erik Mollnes, Sebastian Schaaf, Robert Schwab, Christian P. Strassburg, Ingo G. H. Schmidt-Wolf, Bernd Giebel, Frank Hildebrand, Veronika Lukacs-Kornek, Arnulf G. Willms, Miroslaw T. Kornek
Summary: This study investigated the potential of serum-derived extracellular vesicles (EVs) as biomarkers for decision-making in polytrauma. The findings suggest that specific types of small EVs may serve as valuable, non-invasive, and objective biomarkers for assessing and monitoring the severity of polytrauma and associated organ damage.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Zeynep Karagoz, Fiona R. Passanha, Lars Robeerst, Martijn van Griensven, Vanessa L. S. LaPointe, Aurelie Carlier
Summary: Various cell surface receptors, including cadherin-11 and platelet-derived growth factor receptors (PDGFRs), are involved in the differentiation and self-renewal of human mesenchymal stem cells (hMSCs). This study used a computational model to investigate the interactions between cadherin-11 and PDGFRs, and revealed the existence of crosstalk at both receptor and downstream signaling levels. The findings contribute to a better understanding of the effect of cell surface receptors on hMSCs proliferation.
SCIENTIFIC REPORTS
(2023)