Journal
ACTA BIOMATERIALIA
Volume 73, Issue -, Pages 250-262Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.04.024
Keywords
Osteomyelitis; Biofilm; Bone marrow analog; 3D in vitro model
Funding
- contract research 'Bioinspirierte Materialsynthese' of the Baden-Wurttemberg Foundation [BioMatS-14]
- young investigator network (YIN) of the KIT
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In this work, we define the requirements for a human cell-based osteomyelitis model which overcomes the limitations of state of the art animal models. Osteomyelitis is a severe and difficult to treat infection of the bone that develops rapidly, making it difficult to study in humans. We have developed a 3D in vitro model of the bone marrow, comprising a macroporous material, human hematopoietic stem and progenitor cells (HSPCs) and mesenchymal stromal cells (MSCs). Inclusion of biofilms grown on an implant into the model system allowed us to study the effects of postoperative osteomyelitis-inducing bacteria on the bone marrow. The bacteria influenced the myeloid differentiation of HSPCs as well as MSC cytokine expression and the MSC ability to support HSPC maintenance. In conclusion, we provide a new 3D in vitro model which meets all the requirements for investigating the impact of osteomyelitis. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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