Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 2, Pages 1474-1479Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b13183
Keywords
two photon lithography; direct laser writing HDL; immunoassay; functional polymers; confocal microscopy
Funding
- Austrian Science Fund (FWF) [P 26461, P 29110-B21, P 25763-B13]
- Interreg European Fund project [ATCZ14]
- Upper Austrian University of Applied Sciences basic funding BF-PolFunk
- Austrian Science Fund (FWF) [P29110] Funding Source: Austrian Science Fund (FWF)
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Multiphoton polymerization (MPP) enables 3D fabrication of micro- and nanoscale devices with complex geometries. Using MPP, we create a 3D platform for protein assays. Elevating the protein-binding sites above the substrate surface allows an optically sectioned readout, minimizing the inevitable background signal from nonspecific protein adsorption at the substrate surface. Two fluorescence-linked immunosorbent assays are demonstrated, the first one relying on pstreptavidin-biotin recognition and the second one on antibody recognition of apolipoprotein Al, a major constituent of high-density lipoprotein particles. Signal-to-noise ratios exceeding 1000 were achieved. The platform has high potential for 3D multiplexed recognition assays with an increased binding surface for on-chip flow cells.
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