Article
Biochemistry & Molecular Biology
Yuri Y. Shevelyov
Summary: Heterochromatin is mainly localized at the nuclear periphery by interacting with the nuclear lamina and nuclear pore complexes. Recent studies have shown that these interactions play a crucial role in maintaining its peripheral localization, and there are differences in chromatin interactions with the nuclear envelope in cell populations and individual cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yuexia Wang, Khurts Shilagardi, Trunee Hsu, Kamsi O. Odinammadu, Takamitsu Maruyama, Wei Wu, Chyuan-Sheng Lin, Christopher B. Damoci, Eric D. Spear, Ji-Yeon Shin, Wei Hsu, Susan Michaelis, Howard J. Worman
Summary: Prelamin A, a farnesylated precursor of lamin A, can cause progeria syndrome when accumulated as progerin. Mutations in ZMPSTE24, the processing enzyme of prelamin A, can lead to progeroid disorders. This study generated a mouse model with permanently farnesylated prelamin A and found that these mice have extended lifespan and exhibit skeletal and nuclear defects during physiological aging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Li-Chun Cheng, Xi Zhang, Kanishk Abhinav, Julie A. Nguyen, Sabyasachi Baboo, Salvador Martinez-Bartolome, Tess C. Branon, Alice Y. Ting, Esther Loose, John R. Yates, Larry Gerace
Summary: By using engineered biotin ligase TurboID and quantitative proteomics, this study explored the neighborhoods of Emerin and LBR in cultured mouse embryonic fibroblasts. The analysis revealed 232 high confidence proximity partners interacting selectively with Emerin and/or LBR, with 49 shared by both.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Cell Biology
Lisa Laemmerhirt, Melanie Kappelmann-Fenzl, Stefan Fischer, Michaela Pommer, Tom Zimmermann, Viola Kluge, Alexander Matthies, Silke Kuphal, Anja Katrin Bosserhoff
Summary: Modifications in nuclear structures of cells play a role in diseases, including cancer. The role of nuclear lamina and related proteins in malignant melanoma is unclear. This study investigates the functional effects of dysregulated nuclear lamin B1 (LMNB1) and its nuclear receptor (LBR). It reveals their involvement in nuclear processes and their impact on senescence and chromatin state in melanoma cells. These findings suggest that LMNB1 and LBR are functionally relevant for melanoma progression.
Review
Oncology
Maddison Rose, Joshua T. Burgess, Kenneth O'Byrne, Derek J. Richard, Emma Bolderson
Summary: This review provides an overview of the potential role and possible applications of inner nuclear membrane proteins in cancer therapeutics.
CANCER AND METASTASIS REVIEWS
(2022)
Review
Cell Biology
Hyeong Jae Kim, Peter C. W. Lee, Jeong Hee Hong
Summary: With the discovery of the role of the nuclear envelope protein lamin in human genetic diseases, further diverse roles of lamins have been elucidated. The roles of lamins have been addressed in cellular homeostasis including gene regulation, cell cycle, cellular senescence, adipogenesis, bone remodeling as well as modulation of cancer biology. The modulatory roles of lamin-A/C in stem cell differentiation, skin, cardiac regulation, and oncology have also been elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Pathology
Ji-Yeon Shin, Howard J. Worman
Summary: Nuclear envelope is composed of various components and mutations in genes encoding these components can lead to laminopathies, a group of diseases that selectively affect different tissues or organs. The mechanisms underlying these diseases are still unclear and current hypotheses focus on the impact of nuclear envelope alterations on gene expression and cellular mechanics.
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE
(2022)
Article
Cell Biology
Amar N. Mirza, Fernanda Gonzalez, Sierra K. Ha, Anthony E. Oro
Summary: The nucleoskeleton and TFs have reciprocal impacts on each other, with LEM proteins playing a key role in dynamic TF trafficking pathways. The nuclear lamina serves not only as a refuge for TF accumulation but also for rapid mobilization and signal stability.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Wei-Qi Lin, Zhen-Kai Ngian, Tong-Wey Koh, Chin-Tong Ong
Summary: This study found that reduced levels of nuclear lamin-B protein in Drosophila coincided with a decline in locomotor activity and stress resistance. Tissue-specific expression of lamin-B may regulate different aspects of animal physiology during aging.
Review
Cell Biology
Brandt Warecki, William Sullivan
Summary: A conserved feature of higher eukaryotes is that centromeres are embedded in heterochromatin. This tight association is essential for proper cell division and nuclear envelope formation.
Review
Cell Biology
Aurelie Bellanger, Julia Madsen-osterbye, Natalia M. Galigniana, Philippe Collas
Summary: Cellular senescence and cancer initiation are associated with alterations in the nuclear envelope, heterochromatin reorganization, and massive reprogramming of the epigenome. Changes in chromatin organization and the epigenome affecting lamina-associated domains (LADs) and related genomic domains play crucial roles in senescence and cancer.
Article
Medicine, Research & Experimental
Jing-Qiang Huang, He-Feng Li, Jing Zhu, Jun-Wei Song, Xian-Bin Zhang, Peng Gong, Qiu-Yu Liu, Chun-Hui Zhou, Liang Wang, Li-Yun Gong
Summary: SRPK1 is overexpressed in colon cancer and correlated with clinical stage and TNM classifications. High levels of SRPK1 are associated with poor prognosis. Mechanistically, SRPK1 enhances the anti-apoptosis ability of colon cancer cells under oxaliplatin treatment by promoting NF-κB pathway activation.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Plant Sciences
Joseph F. McKenna, Hardeep K. Gumber, Zachary M. Turpin, Alexis M. Jalovec, Andre C. Kartick, Katja Graumann, Hank W. Bass
Summary: The study demonstrates that nucleoskeletal proteins NCH1, NCH2, and MKAKU41 in maize have characteristic properties of LINC-associated plant nucleoskeletal proteins, impacting the nuclear periphery structure and overall nuclear architecture.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Cell Biology
Eric C. Schirmer, Leena Latonen, Sylvain Tollis
Summary: Research suggests that changes in nuclear size are associated with tumor metastasis. Recent studies have found that reversing tumor type-dependent nuclear size changes is correlated with reduced cell migration and invasion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
A. K. Balaji, Santam Saha, Shruti Deshpande, Darshini Poola, Kundan Sengupta
Summary: Dysregulation of chromatin remodeling and nuclear envelope proteins plays a significant role in cancer development. Recent advances in single-cell sequencing, imaging technologies, and data mining approaches enable the design of small molecules to selectively inhibit cancer cell growth and proliferation in a genome- and epigenome-specific manner.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Maria Kourti, Georgia Ikonomou, Nikolaos-Nikiforos Giakoumakis, Maria Anna Rapsomaniki, Ulf Landegren, Symeon Siniossoglou, Zoi Lygerou, George Simos, Ilias Mylonis
CELLULAR SIGNALLING
(2015)
Article
Cell Biology
Ilias Mylonis, Maria Kourti, Martina Samiotaki, George Panayotou, George Simos
JOURNAL OF CELL SCIENCE
(2017)
Article
Cell Biology
Evanthia Pangou, Christina Befani, Ilias Mylonis, Martina Samiotaki, George Panayotou, George Simos, Panagiotis Liakos
JOURNAL OF CELL SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Sotiria Drakouli, Aggeliki Lyberopoulou, Maria Papathanassiou, Ilias Mylonis, Eleni Georgatsou
Article
Biochemistry & Molecular Biology
Eleni-Anastasia Triantafyllou, Eleni Georgatsou, Ilias Mylonis, George Simos, Efrosyni Paraskeva
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2018)
Article
Biochemistry & Molecular Biology
Angeliki Karagiota, Maria Kourti, George Simos, Ilias Mylonis
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Angeliki Karagiota, Ilias Mylonis, George Simos, Georgia Chachami
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Review
Cell Biology
Ilias Mylonis, George Simos, Efrosyni Paraskeva
Review
Oncology
Ilias Mylonis, Georgia Chachami, George Simos
Summary: Hypoxia inducible transcription factors (HIFs) play a critical role in enabling cancer cells to thrive in low oxygen conditions. Inhibiting HIFs can prevent cancer cell growth and metastasis, with peptide HIF inhibitors showing potential as highly selective therapeutic agents. This review highlights the importance of targeting HIFs in cancer treatment and the promising development of peptide inhibitors.
Article
Oncology
Kreon Koukoulas, Antonis Giakountis, Angeliki Karagiota, Martina Samiotaki, George Panayotou, George Simos, Ilias Mylonis
Summary: ERK-mediated phosphorylation of HIF-1 alpha enhances its interaction with NPM1, which influences the transcriptional activation of HIF-1 target genes. NPM1 and HIF-1 co-regulate genes enriched in different cancer types, and their expression correlates with hypoxic tumor status and patient prognosis.
MOLECULAR ONCOLOGY
(2021)
Review
Oncology
Ioanna-Maria Gkotinakou, Ilias Mylonis, Andreas Tsakalof
Summary: Vitamin D functions as a nutrient as well as a hormone, regulating various physiological functions. The relationship between calcitriol and HIF signaling in cancer development and treatment needs further investigation.
Article
Biochemistry & Molecular Biology
Chrysa Taze, Sotiria Drakouli, Martina Samiotaki, George Panayotou, George Simos, Eleni Georgatsou, Ilias Mylonis
Summary: The cellular response to hypoxia involves both HIF-dependent transcriptional reprogramming and less explored transcription-independent processes, such as alternative splicing of VEGFA transcript. This study shows that the splicing machinery is reorganized after short-term hypoxia, triggered by ROS production and intranuclear redistribution of nucleoskeletal proteins SAFB1/2. This remodeling of the nuclear architecture promotes the production of splicing variants that facilitate adaptation to hypoxia.
Article
Cell Biology
Anastasia Koukiali, Makrina Daniilidou, Ilias Mylonis, Thomas Giannakouros, Eleni Nikolakaki
Summary: Although SRPKs were discovered nearly 30 years ago, our understanding of their mode of regulation is still limited. In this study, we identified TAF15 as a novel SRPK1-interacting protein and demonstrated that the C-terminal RGG domain of TAF15 can downregulate SRPK1 activity. Peptides comprising the RGG repeats of nucleolin, HNRPU, and HNRNPA2B1 were also shown to inhibit SRPK1 activity, suggesting that negative regulation of SRPK1 activity might be a key biochemical property of RGG motif-containing proteins.
Review
Cell Biology
Angelos Yfantis, Ilias Mylonis, Georgia Chachami, Marios Nikolaidis, Grigorios D. Amoutzias, Efrosyni Paraskeva, George Simos
Summary: HIF-1 is a key regulator in cell survival under low oxygen conditions and its interaction with chromatin and transcriptional machinery is still being investigated. This review examines the co-regulators of HIF-1 and their impact on the expression of HIF-1 direct target genes in response to hypoxia, with the aim of identifying potential targets for anticancer therapy.