Article
Medical Laboratory Technology
Yuqi Wang, Ling Li, Ping Li
Summary: The association between gestational diabetes mellitus (GDM) and single nucleotide polymorphisms (SNPs) has been widely studied. SNPs can provide insights into the pathogenesis of GDM, help predict the risk of GDM, and guide the management of GDM patients. This review focuses on recent studies investigating the association between SNPs and GDM, identifying several SNPs that have been associated with GDM. However, further research is needed to explore the role of SNPs in the prediction, diagnosis, treatment, and prognosis of GDM in diverse ethnic populations.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Tengfei Yuan, Yan Li
Summary: Previous studies have shown associations between steroids and gestational diabetes mellitus (GDM). This study aimed to comprehensively analyze steroid profiling in Chinese women with GDM during the third trimester of pregnancy. Results indicated significant differences in steroids and product-to-precursor ratios between GDM and control groups, with certain genetic variants predisposing Chinese women to GDM.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Polina Popova, Alexandra A. Klyushina, Lyudmila B. Vasilyeva, Alexandra S. Tkachuk, Elena A. Vasukova, Anna D. Anopova, Evgenii A. Pustozerov, Inga Gorelova, Ekaterina N. Kravchuk, O. Li, Tatiana M. Pervunina, Anna A. Kostareva, Elena N. Grineva
Summary: Among 11 SNPs associated with T2D and/or GDM in other populations, significant association with GDM was confirmed for two variants in MTNR1B in Russian women. However, the value of these variants in identifying GDM cases was limited.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Nutrition & Dietetics
Siyu Lai, Dandan Yan, Jie Xu, Xiangtian Yu, Jingyi Guo, Xiangnan Fang, Mengyang Tang, Rong Zhang, Hong Zhang, Weiping Jia, Mingjuan Luo, Cheng Hu
Summary: In this study, the genetic effects of four key genes (CYP2C8, CYP2C9, CYP2J2, and EPHX2) on the processing and degradation pathways of epoxyeicosatrienoic acid in gestational diabetes mellitus (GDM) and metabolic traits were explored in a Chinese population. Significant associations were found between GDM susceptibility and 12 single nucleotide polymorphisms (SNPs) of these genes. A genetic risk score model was constructed to assess GDM risk. Additionally, a significant interaction between CYP2J2 and CYP2C8 in GDM was observed. These findings are important for understanding the genetic mechanisms underlying GDM and predicting GDM risk.
Review
Endocrinology & Metabolism
Yumeng Tian, Ping Li
Summary: This article reviews the research progress in the use of genetic risk score (GRS) in diabetes mellitus in recent years and discusses future prospects.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Chenyu Jiang, Mi Zhou, Huai Bai, Meng Chen, Chunyi Yang, Kaifeng Hu, Yujie Wu, Qingqing Liu, Yangyu Zhao, Xinghui Liu, Ping Fan
Summary: This study found that the MPO G-463A genetic variation is associated with an increased risk of GDM, while the CYBA C242T genetic variation is not. These two genetic polymorphisms may be linked to obesity, dyslipidemia, insulin resistance, and oxidative stress.
ENDOCRINE CONNECTIONS
(2023)
Article
Medicine, General & Internal
Kymberleigh A. Pagel, Hoyin Chu, Rashika Ramola, Rafael F. Guerrero, Judith H. Chung, Samuel Parry, Uma M. Reddy, Robert M. Silver, Jonathan G. Steller, Lynn M. Yee, Ronald J. Wapner, Matthew W. Hahn, Sriraam Natarajan, David M. Haas, Predrag Radivojac
Summary: This cohort study found that the addition of polygenic risk scores (PRS) was associated with the stratified risk of gestational diabetes (GD) diagnosis among high-risk patient subgroups, suggesting the benefits of targeted PRS ascertainment to encourage early intervention.
Article
Endocrinology & Metabolism
Ana Ramos-Levi, Ana Barabash, Johanna Valerio, Nuria Garcia de la Torre, Leire Mendizabal, Mirella Zulueta, Maria Paz de Miguel, Angel Diaz, Alejandra Duran, Cristina Familiar, Ines Jimenez, Laura Del Valle, Veronica Melero, Inmaculada Moraga, Miguel A. Herraiz, Maria Jose Torrejon, Maddi Arregi, Laureano Simon, Miguel A. Rubio, Alfonso L. Calle-Pascual
Summary: This study identified a core set of SNPs associated with diabetes and GDM in a large cohort of patients from two main ethnicities. The identification of these genetic variants, even in the context of a nutritional intervention, is useful for designing preventive and therapeutic strategies.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Qi Wu, Yanmin Chen, Menglin Zhou, Mengting Liu, Lixia Zhang, Zhaoxia Liang, Danqing Chen
Summary: This study developed and verified an early pregnancy prediction model of gestational diabetes mellitus (GDM) based on genetic variants and clinical characteristics. The model showed good predictive power in the trial cohort, test cohort, and validation cohort. It can help identify high-risk individuals for GDM in early pregnancy and provide timely interventions.
DIABETOLOGY & METABOLIC SYNDROME
(2022)
Article
Endocrinology & Metabolism
Manning Zhu, Yaer Lv, Yanqing Peng, Yingnan Wu, Yanan Feng, Tianshuang Jia, Songcheng Xu, Songxue Li, Wei Wang, Jiawei Tian, Litao Sun
Summary: This study investigated the associations of GCKR and ADIPOQ gene variants with the risk of gestational diabetes mellitus (GDM) in Chinese women. The results showed that the GCKR rs1260326 variant was significantly associated with GDM and related clinical indicators. The association between ADIPOQ gene variants and GDM was weaker.
ACTA DIABETOLOGICA
(2023)
Article
Endocrinology & Metabolism
Qiaoli Zeng, Taili Yang, Wenfeng Wei, Dehua Zou, Yue Wei, Fengqiong Han, Jieyun He, Jinzhi Huang, Runmin Guo
Summary: The study found that GLO1 rs1130534 was associated with an increased susceptibility to GDM and higher blood glucose levels, while GLO1 rs4746 was associated with a decreased risk of GDM. The rs1781735 variant was linked to maternal glyoxal accumulation and subsequent weight gain in neonates.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Tianxiao Zhang, Longrui Zhao, Shujin Wang, Juan Liu, Ying Chang, Louyan Ma, Jia Feng, Yu Niu
Summary: This study found that NUS1 and GP2 genes are associated with the susceptibility to gestational diabetes mellitus (GDM) in Chinese Han population. Two SNPs were significantly associated with the risk of GDM, as well as related biomedical indexes. Differences in serum levels of protein NUS1 and GP2 were identified among groups with different genotypes of the SNPs.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Gongchen Huang, Qiulian Liang, Yukun Wang, Linyuan Qin, Haili Yang, Lin Lin, Xiangyuan Yu
Summary: This study found a significant relationship between ACE2 genetic variants and the risk of gestational diabetes mellitus (GDM) in a southern Chinese population. The association appears to be mediated through gene-gene and gene-environment interactions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Physiology
B. Ortega-Contreras, A. Armella, J. Appel, D. Mennickent, J. Araya, M. Gonzalez, E. Castro, A. M. Obregon, L. Lamperti, J. Gutierrez, E. Guzman-Gutierrez
Summary: Gestational Diabetes Mellitus (GDM) is a pathological condition in pregnant women that is associated with genetic predisposition. This review explores the potential effects of various polymorphisms in genes on the development of GDM.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Immunology
Hongying Liu, Aizhong Liu, Atipatsa C. Kaminga, Judy McDonald, Shi Wu Wen, Xiongfeng Pan
Summary: This meta-analysis indicates the presence of abnormal concentrations of various chemokines in patients with GDM, which are closely associated with the development of GDM. Additionally, the subgroup analysis highlights the importance of investigating the potential mechanisms underlying this association to develop individualized clinical and therapeutic strategies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Diogo Henrique Kita, Nathalie Guragossian, Ingrid Fatima Zattoni, Vivian Rotuno Moure, Fabiane Gomes de Moraes Rego, Sabrina Lusvarghi, Thomas Moulenat, Billel Belhani, Geraldo Picheth, Sofiane Bouacida, Zouhair Bouaziz, Christelle Marminon, Malika Berredjem, Joachim Jose, Marcos Brown Goncalves, Suresh V. Ambudkar, Glaucio Valdameri, Marc Le Borgne
Summary: A new generation of indeno[1,2-b]indole compounds were synthesized and tested as ABCG2 inhibitors, showing potent inhibition activity and ability to fully chemosensitize cancer cells. These compounds exert their effects by stimulating ABCG2 ATPase activity and stabilizing the protein structure.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Inorganic & Nuclear
Gabriel B. Baptistella, Graciele C. M. Manica, Susan W. de Souza, Francielli S. Santana, Lucas G. Fachini, David L. Hughes, Eduardo L. de Sa, Geraldo Picheth, Jaisa F. Soares, Fabiane G. M. Rego, Giovana G. Nunes
Summary: A centrosymmetric oxidovanadium(IV) complex was synthesized and characterized through various analyses, including cytotoxicity evaluation and investigation of electronic structure. The results indicate the potential anti-diabetic activity of the complex in a hyperglycemic environment.
Article
Biochemistry & Molecular Biology
Andressa Chequin, Luiz E. Costa, Felipe F. de Campos, Angie D. B. Moncada, Lucas T. F. de Lima, Lucas R. Sledz, Guilherme F. Picheth, Eliana R. Adami, Alexandra Acco, Marcos B. Goncalves, Graciele C. M. Manica, Glaucio Valdameri, Lucia de Noronha, Jose E. Q. Telles, Elisa H. F. Jandrey, Erico T. Costa, Fabricio F. Costa, Emanuel M. de Souza, Edneia A. S. Ramos, Giseli Klassen
Summary: The study explored the potential of repurposing the diabetes type II drug liraglutide to modulate epigenetic modifications in breast cancer cells, showing significant reductions in cell viability, migration, and DNA methylation levels. The in vivo experiments demonstrated that liraglutide was effective in reducing tumor growth, indicating its potential as a new adjuvant treatment for breast cancer.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Ingrid Fatima Zattoni, Thales Kronenberger, Diogo Henrique Kita, Lais Danciguer Guanaes, Matheus Murmel Guimaraes, Larissa de Oliveira Prado, Melanie Ziasch, Luis C. Vesga, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Marcos Brown Goncalves, Miguel D. Noseda, Diogo R. B. Ducatti, Antti Poso, Robert W. Robey, Suresh Ambudkar, Vivian Rotuno Moure, Alan Guilherme Goncalves, Glaucio Valdameri
Summary: A newly discovered porphyrin derivative (4B) was identified as an inhibitor of the ABCG2 transporter, capable of overcoming multidrug resistance in vitro and showing selective inhibition towards ABCG2 without being transported by it.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Oncology
Claudia Martins Galindo, Fernando Augusto de Oliveira Ganzella, Giseli Klassen, Edneia Amancio de Souza Ramos, Alexandra Acco
Summary: PFKFB3 is a critical enzyme that supports glucose catabolism and plays a role in the progression of various cancers. It is important for sustaining glycolysis in the tumor environment, promoting metabolic reprogramming, cell proliferation, DNA repair, and drug resistance. Breast cancer has unique characteristics that drive the expression of PFKFB3. This enzyme acts as a convergence point for multiple growth-promoting and oncogenic signaling pathways.
CLINICAL BREAST CANCER
(2022)
Article
Biochemistry & Molecular Biology
Diogo Henrique Kita, Gisele Alves de Andrade, Juliana Morais Missina, Kahoana Postal, Viktor Kalbermatter Boell, Francielli Sousa Santana, Ingrid Fatima Zattoni, Isadora da Silva Zanzarini, Vivian Rotuno Moure, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Emanuel Maltempi de Souza, David A. Mitchell, Suresh Ambudkar, Giovana Gioppo Nunes, Glaucio Valdameri
Summary: The study found that polyoxovanadates can serve as inhibitors of ABC drug transporters, selectively inhibiting P-glycoprotein with V-10 and V-18 being the most promising compounds.
Article
Biotechnology & Applied Microbiology
I. F. Zattoni, L. F. Huergo, E. C. M. Gerhardt, J. M. Nardin, A. M. F. dos Santos, F. G. M. Rego, G. Picheth, V. R. Moure, G. Valdameri
Summary: Flow cytometry has been used as a promising technique for detecting SARS-CoV-2 antibodies. In this study, a novel strategy was developed to simultaneously detect IgG, IgM, and IgA using a covalently bound nucleocapsid protein and fluorophore-conjugated secondary antibodies. The multiplexed assay showed high sensitivity and specificity, allowing for efficient discrimination between COVID-19 negative and positive samples.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Veronica S. Pires, Fernando A. de O. Ganzella, Guilherme A. Minozzo, Luciana L. Dias de Castro, Angie D. B. Moncada, Giseli Klassen, Edneia A. S. Ramos, Marcelo B. Molento
Summary: The study analyzed the DNA methylation profile of the SLC11a1 gene in naturally infected horses and found a correlation between high methylation of island 2 and cyathostomin fecal egg count. Animals with high methylation rates may exhibit a decrease in fecal egg count over time. This suggests the possibility of epigenetic regulation of the SLC11a1 gene in horses.
Article
Microbiology
Marcelo S. Conzentino, Ana C. A. Goncalves, Nigella M. Paula, Fabiane G. M. Rego, Dalila L. Zanette, Mateus N. Aoki, Jeanine M. Nardin, Luciano Fernandes Huergo
Summary: This article describes an immunological assay for detecting immune response to SARS-CoV-2 Spike Receptor Binding Domain (RBD) antigen in humans. The method is simple, low cost, and high throughput, and can be used to monitor the levels of protective antibodies in response to infection and/or immunization in the population.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Guilherme F. Picheth, Fernando A. de O. Ganzella, Joao O. C. Filizzola, Yan K. Canquerino, Gabriela C. Cardoso, Michelle B. Collini, Leonardo B. Colauto, Maria Cristina Figueroa-Magalhaes, Edneia A. S. R. Cavalieri, Giseli Klassen
Summary: This review discusses newly developed approaches and strategies to improve treatment and overcome resistance mechanisms associated with breast cancer. It highlights the use of ligands with selective or tailored affinity to increase therapeutic efficiency and reduce side effects and recurrence.
Article
Agriculture, Dairy & Animal Science
Luciana L. Dias De Castro, Gerson A. Oliveira Junior, Bruno Costa Perez, Minos E. Carvalho, Edneia A. De Souza Ramos, Jose Bento S. Ferraz, Marcelo Beltrao Molento
Summary: This study identified genomic regions associated with Cyathostomins infection in horses through genomic-wide association analysis. Candidate genes related to fecal egg count and hematological parameters were discovered, providing insights for animal breeding activities and reducing losses caused by parasite infections.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thanise Pitelli de Nigro, Graciele Cristiane More Manica, Susan Webber de Souza, Carlos Henrique Alves Jesus, Rubia Camila Ronqui Bottini, Juliana Morais Missina, Glaucio Valdameri, Giovana Gioppo Nunes, Joice Maria da Cunha, Geraldo Picheth, Fabiane Gomes de Moraes Rego
Summary: Diabetes mellitus is a complex and heterogeneous disease associated with hyperglycemia, leading to mortality and reduced life expectancy. This study evaluated the effects of a vanadium complex, [VO(bpy)(mal)]·H2O (complex A), in human hepatocarcinoma cells and streptozotocin-induced diabetic rats. The results showed that complex A had low cytotoxic effects, increased glucose uptake, and improved glycemia when administered orally in combination with insulin. These findings suggest that complex A has potential as a promising adjuvant treatment for diabetes.
Article
Multidisciplinary Sciences
Isadora da Silva Zanzarini, Gustavo Barbosa, Larissa de Oliveira Prado, Ingrid Fatima Zattoni, Gustavo Da Paz, Ademir Luiz Prado, Waldemar Volanski, Marcos Dinis Lavarda, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Vivian Rotuno Moure, Glaucio Valdameri
Summary: This paper introduces a novel, simple, and low-cost device for in vitro photodynamic therapy (PDT) assays. The device utilizes conventional programmable LEDs, an LCD module, and a light sensor connected to a commercial microcontroller board. Experimental results demonstrate that the device enhances the cytotoxic effect of the photosensitizer verteporfin through reactive oxygen species (ROS) mediated mechanism. The prototype described in this work is validated by comparing the results with a commercial PDT device, showing its potential as an alternative for in vitro studies of PDT.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Lucas Trevisan Franca de Lima, Fernando Augusto de Oliveira Ganzella, Gabriela Casani Cardoso, Veronica dos Santos Pires, Andressa Chequin, Giulia Luiza Santos, Karin Braun-Prado, Claudia Martins Galindo, Odair Braz Braz Junior, Marcelo Beltrao Molento, Alexandra Acco, Eliana Rezende Adami, Erico Tosoni Costa, Celia Regina Cavichiolo Franco, Giseli Klassen, Edneia Amancio de Souza Ramos
Summary: The monoterpene L-carvone (CRV) found in spearmint has been shown to inhibit adhesion, migration, and invasion of breast cancer cells in vitro and suppress tumor growth in mice. Mechanistic studies revealed that CRV disrupts focal adhesion by modulating the beta 1-integrin/FAK signaling pathway.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Pharmacology & Pharmacy
Glaucio Valdameri, Diogo Henrique Kita, Julia de Paula Dutra, Diego Lima Gomes, Arun Kumar Tonduru, Thales Kronenberger, Bruno Gavinho, Izadora Volpato Rossi, Mariana Mazetto de Carvalho, Basile Peres, Ingrid Fatima Zattoni, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Rilton Alves de Freitas, Antti Poso, Suresh V. Ambudkar, Marcel Ramirez, Ahcene Boumendjel, Vivian Rotuno Moure
Summary: Inhibition of ABC transporters is a promising strategy to overcome multidrug resistance in cancer. This study identified a potent ABCG2 inhibitor called chromone 4a (C4a) that exhibits selectivity towards ABCG2. C4a effectively inhibited the efflux of different substrates mediated by ABCG2 and showed potential for drug delivery using liposomes and extracellular vesicles (EVs) in overcoming poor water solubility.