Article
Chemistry, Physical
Kateryna Vus, Uliana Tarabara, Ihor Danylenko, Yaroslav Pirko, Tetiana Krupodorova, Alla Yemets, Yaroslav Blume, Vitalii Turchenko, Dmytro Klymchuk, Petro Smertenko, Olga Zhytniakivska, Valeriya Trusova, Sergii Petrushenko, Sergey Bogatyrenko, Galyna Gorbenko
Summary: Silver nanoparticles (SNPs) synthesized extracellularly using extracts of different genus Pleurotus fungi samples showed pronounced anti-amyloid effect in vitro, suggesting their potential application as effective anti-amyloid agents.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Cai-Ling Shen, Yu-Hsuan Wu, Ting-Hao Zhang, Ling-Hsien Tu
Summary: More than 30 human peptides or proteins have been found to form amyloid fibrils, which are associated with various human diseases. However, there is currently no cure for amyloidosis. In this study, iron oxide nanoparticles were used to inhibit the aggregation of human calcitonin (hCT) and dissociate preformed amyloid fibrils, offering a potential therapeutic strategy for amyloidosis treatment.
Article
Biochemical Research Methods
Hitesh Mandal, Arbin Basak, Taraka Prabhu, Vidyalatha Kolli, Nandini Sarkar
Summary: In this study, various hydroxy-benzoic phytochemicals were investigated for their effects on the formation and disaggregation of HEWL amyloids. The compounds showed significant inhibitory and disaggregation effects on the amyloids, with interactions involving hydrogen bonding. Computational docking studies provided insights into the molecular interactions between the selected compounds and HEWL, highlighting their potential as therapeutic agents against amyloidosis.
CURRENT PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Mantas Ziaunys, Andrius Sakalauskas, Kamile Mikalauskaite, Vytautas Smirnovas
Summary: Protein aggregation is considered a major cause of several neurodegenerative diseases. Despite progress in amyloid research, the process of fibrillization remains not fully understood. Tracking insulin aggregation using ThT reveals anomalous double-sigmoidal kinetics, likely related to ThT-positive intermediates, which do not significantly alter the overall aggregation process.
Article
Chemistry, Physical
Akshat M. Desai, Shrishti P. Pandey, Prabhat K. x Singh
Summary: This study investigated the influence of different counter-anions on the aggregation of the cationic amyloid sensing probe Thioflavin-T (ThT), revealing distinct ion behaviors in the organization process. Optical properties of the salt-induced aggregates were characterized using detailed emission and absorption measurements, and the formation of contact ion pairs was explained through the theory of matching water affinity. These findings suggest that the selection of counterions with appropriate sizes can induce aggregation of ThT and potentially help in detecting false positive signals in high ionic strength environments.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Timothy S. Chisholm, Christopher A. Hunter
Summary: This study reports a method to characterize the morphology of alpha-synuclein fibrils by profiling multiple ligand binding sites on their surfaces. By using various competition binding assays, seven different types of binding sites were identified on four different morphologies of alpha-synuclein fibrils. Similar binding sites on different fibrils showed significantly different affinities for ligands. The information on binding sites and ligand interactions was combined to construct individual profiles for different alpha-synuclein fibrils.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Karina Abramov-Harpaz, Yifat Miller
Summary: This study provides insights into the molecular mechanisms of insulin degrading enzyme (IDE) activity on encapsulating different forms of amyloid beta (A beta) dimers. IDE acts as a 'dead-end' chaperone protein for fibril-like A beta dimers and successfully impedes the contacts between monomers. However, IDE's inhibitory activity on random coil/alpha-helix dimers depends on the stability of the dimer.
Article
Chemistry, Physical
Olga Zhytniakivska, Atanas Kurutos, Mykhailo Shchuka, Kateryna Vus, Uliana Tarabara, Valeriya Trusova, Galyna Gorbenko
Summary: This study demonstrates that the Fo spacing diaeresis rster resonance energy transfer (FRET) technique can differentiate between the non-fibrillized and fibrillar states of insulin. The presence of cyanine dyes causes a significant decrease in ThT fluorescence, with the magnitude of this effect depending on the cyanine chemical structure.
CHEMICAL PHYSICS LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Patricia Bondia, Cristina Flors, Joaquim Torra
Summary: Thioflavin T (ThT), commonly used as a fluorescent probe for amyloid detection, has been discovered to exhibit photodynamic activity against bacterial biofilms. It disrupts the complex architecture of biofilm matrix and efficiently inactivates bacterial cells, while also synergistically enhancing the activity of conventional antimicrobials.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Song Liu, Lingli Bu, Yuming Zhang, Jiangyu Yan, Ling Li, Guorui Li, Zhibin Song, Jing Huang
Summary: This study describes two coumarin-quinazolinone conjugates, CQ and MeCQ, which exhibit significantly different behaviors in cellular context due to their different interactions with G-quadruplex (G4) DNA. CQ shows bright fluorescence in living cells while MeCQ is nearly undetectable.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Shantanu Sen, Rafat Ali, Akanksha Onkar, Subramaniam Ganesh, Sandeep Verma
Summary: This review discusses the methods for decelerating insulin aggregation, including excipient additions and intrinsic modifications to enhance its stability and usage.
Article
Biochemistry & Molecular Biology
Bilal Javed, Xinyi Zhao, Daxiang Cui, James Curtin, Furong Tian
Summary: The study synthesized Lignin-g-p (NIPAM-co-DMAEMA) gold nanogel loaded with curcumin and piperine to treat GBM, which exhibited enhanced anticancer properties, improved drug toxicity and release, and induced apoptosis through endocytic pathways. The experimental evidence suggests that the combinatorial curcumin-piperine gold nanogels have the potential to overcome the limitations of conventional chemotherapeutic treatments for glioma cells.
Article
Chemistry, Multidisciplinary
Xuejun Cheng, Yingming Pu, Songtao Ye, Xiao Xiao, Xin Zhang, Hongyu Chen
Summary: This paper demonstrates the capability to monitor the solvent exchange process within silica micropores with millisecond time resolution by embedding rotor-based fluorophores. The results validate the effectiveness and sensitivity of this method for characterizing diffusion in silica micropores.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Kien Hong Trinh, Ulhas Sopanrao Kadam, Jinnan Song, Yuhan Cho, Chang Ho Kang, Kyun Oh Lee, Chae Oh Lim, Woo Sik Chung, Jong Chan Hong
Summary: The study successfully isolated DNA aptamers that can bind to fenitrothion through the SELEX process. The FenA2 aptamer exhibited high detection sensitivity and specificity, providing a potential label-free detection mode for real-time detection of fenitrothion. This opens up new possibilities for analytical tools in various samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Torsten John, Lisandra L. L. Martin, Bernd Abel
Summary: This review discusses the impact of surface interfaces on the structure and aggregation mechanisms of peptides and proteins, particularly their association with amyloid fibril formation and neurodegenerative diseases. Research findings demonstrate that surface interfaces can both accelerate and inhibit peptide self-assembly, with adsorption typically promoting aggregation. The review introduces experimental and theoretical models for better understanding peptide self-assembly at hard and soft matter interfaces, and proposes relationships between biological interfaces and amyloid fibril formation.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Vandana Dahiya, Bibin G. Anand, Karunakar Kar, Samanwita Pal
Summary: The analysis of the interaction between organophosphate pesticides (OP) and bovine serum albumin (BSA) through STD-NMR and molecular docking provided insights into the binding interactions and determined the binding affinities of the complexes. The research revealed high binding affinities through non-covalent interactions between these OP and BSA complexes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Physical
Kailash P. Prajapati, Ayoushna Panigrahi, Sampreeta Purohit, Masihuzzaman Ansari, Kriti Dubey, Rajendra Kumar Behera, Bibin G. Anand, Karunakar Kar
Summary: The study synthesized gold nanoparticles coated with osmolytes, which were found to inhibit temperature-induced amyloid aggregation of insulin. The nanoparticles interacted directly with insulin, protecting it from aggregation-prone domains under extreme conditions, suggesting potential for antiamyloid nanoformulations.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Vibha Kaushik, Daniela Bruennert, Eva-Maria Hanschmann, Phulwanti Kumari Sharma, Bibin G. Anand, Karunakar Kar, Suneel Kateriya, Pankaj Goyal
Summary: The study found that cofilin-1 has an intrinsic tendency to aggregate and form amyloid-like structures in vitro. Oxidation at Cys-80 makes cofilin-1 unstable and leads to a partial loss of protein structure. The results support the hypothesis that cofilin-1 aggregation may play a role in cofilin-mediated pathology and the progression of several amyloid-linked diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Dolat Singh Shekhawat, Pratibha Singh, Vikash Chandra Janu, Praveen Sharma, Kuldeep Singh
Summary: This study evaluated the relationship between maternal and umbilical cord serum aluminum levels and infant neurodevelopment. The results showed a significant correlation between maternal and cord serum aluminum levels, but no correlation with infant neurodevelopment.
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
(2023)
Article
Engineering, Biomedical
Bibin Anand, Qi Wu, Maryam Nakhaei-Nejad, Govindarajan Karthivashan, Lyudmyla Dorosh, Sara Amidian, Abhishek Dahal, Xiuju Li, Maria Stepanova, Holger Wille, Fabrizio Giuliani, Satyabrata Kar
Summary: Native PLGA nanoparticles show therapeutic potential in the treatment of Alzheimer's disease by suppressing aggregation of beta-amyloid peptides, triggering their disassembly, reducing phosphorylation of tau protein, enhancing neuronal viability, and attenuating memory deficits and A beta levels in animal models of AD.
BIOACTIVE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
M. Ansari, K. P. Prajapati, B. G. Anand, P. Chaudhuri, S. Mittal, K. Kar
Summary: In nature, hierarchically ordered supramolecular assemblies built from protein building blocks become key elements in the composition of both functional nanostructures and pathological nanostructures. The construction of multiprotein nanoarchitecture is an important research target for innovations in the designing of bioactive nanomaterials. However, the structural and catalytic characteristics of heterogeneous nanofibers formed from diverse proteins remain largely unknown.
MATERIALS TODAY NANO
(2023)
Article
Biochemistry & Molecular Biology
Shikha Mittal, Kailash Prasad Prajapati, Masihuzzaman Ansari, Bibin Gnanadhason Anand, Karunakar Kar
Summary: Curcumin exhibits multiple medical benefits including anticarcinogenic, anti-inflammatory, antibiotic and antiamyloid properties. Its mechanism of action is complicated due to low bio-viability and autooxidation affected by temperature, pH and buffer. The antiamyloid potential of curcumin is mainly attributed to synergistic action with its autooxidized products, which inhibit amyloid aggregation and promote disaggregation. Curcumin also inhibits coaggregation of food proteins and protects against UV-induced protein damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Kailash Prasad Prajapati, Masihuzzaman Ansari, Shikha Mittal, Bibin Gnanadhason Anand, Karunakar Kar
Summary: Recent discoveries on the self-assembly of aromatic amino acids into amyloid-like neurotoxic nanostructures have sparked interest in decoding the molecular mechanisms behind the onset of neurodegeneration. The multicomponent nature of amyloid deposits challenges the existing amyloid cascade hypothesis. Understanding the neurotoxicity of amyloid-like nanostructures formed by aromatic amino acids is crucial for comprehending the etiology of amyloidogenesis.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Kailash Prasad Prajapati, Bibin Gnanadhason Anand, Masihuzzaman Ansari, Ashu Bhan Tiku, Karunakar Kar
Summary: Dietary consumption of Trp is essential for crucial metabolic processes. Abnormal build-up of Trp causes brain-related medical complications. Under physiologically mimicked conditions, Trp self-assembles into amyloid-mimicking nanofibers. These Trp nanofibers have high affinity for native proteins and can induce amyloid formation. They also show toxic effects on neuroblastoma cells. This study provides insights into Trp-related complications and has significance for nanomaterials design.
Article
Biochemistry & Molecular Biology
Qi Wu, Govindarajan Karthivashan, Maryam Nakhaei-Nejad, Bibin G. Anand, Fabrizio Giuliani, Satyabrata Kar
Summary: Biodegradable poly(lactic-co-glycolic acid)(PLGA) nanoparticles have excellent biocompatibility and can be used to deliver drugs to target tissues. This study found that native PLGA without any drug/agent can protect neurons against A beta-induced toxicity and influence the expression of AD-related genes/proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Nathan Gamble, Eleanor Elise Paul, Bibin Anand, Assen Marintchev
Summary: Translation initiation in eukaryotes is dependent on a complex network of interactions, with eIF5 playing a central role in PIC assembly and remodeling. Phosphorylation by CK2 increases eIF5's affinity for eIF1A, shedding light on the mechanisms underlying CK2 stimulation of protein synthesis and cell proliferation. This study also reveals a new contact interface between eIF5-CTD and the OB domain of eIF1A, contributing to the overall affinity between the two proteins.
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kailash Prasad Prajapati, Bibin Gnanadhason Anand, Masihuzzaman Ansari, Mayur Temgire, Ashu Bhan Tiku, Karunakar Kar
Summary: This study demonstrates that dopamine, an important aromatic neuromodulator, can self-assemble into cytotoxic supramolecular nanostructures under physiological conditions, suggesting its potential contribution to dopamine-related disorders.