Journal
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
Volume 23, Issue 3, Pages 597-604Publisher
SPRINGER
DOI: 10.1007/s12298-017-0456-5
Keywords
C. annuum; CanPI7; Transgenic tomato; Proteinase inhibitor; H. armigera
Categories
Funding
- CSIR
- Science and Engineering Research Board, Department of Science & Technology, Government of India, New Delhi [SB/YS/LS-260/2013]
- CSIR under XII five year plan program [BSC0107]
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Plant proteinase inhibitors (PIs) are plant defense proteins and considered as potential candidates for engineering plant resistances against herbivores. Capsicum annuum proteinase inhibitor (CanPI7) is a multi-domain potato type II inhibitor (Pin-II) containing four inhibitory repeat domains (IRD), which target major classes of digestive enzymes in the gut of Helicoverpa armigera larvae. Stable integration and expression of the transgene in T1 transgenic generation, were confirmed by established molecular techniques. Protein extract of transgenic tomato lines showed increased inhibitory activity against H. armigera gut proteinases, supporting those domains of CanPI7 protein to be effective and active. When T1 generation plants were analyzed, they exhibited antibiosis effect against first instar larvae of H. armigera. Further, larvae fed on transgenic tomato leaves showed delayed growth relative to larvae fed on control plants, but did not change mortality rates significantly. Thus, better crop protection can be achieved in transgenic tomato by overexpression of multi-domain proteinase inhibitor CanPI7 gene against H. armigera larvae.
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