Article
Biotechnology & Applied Microbiology
Jie Zhou, Fengling Guo, Chuangdong Qi, Jiaping Fu, Ying Xiao, Jinping Wu
Summary: This study aimed to optimize the protocol for acclimatization of tissue culture ginger plantlets. By adjusting the conditions of light, temperature, humidity, and substrate, successful survival and rooting of tissue culture ginger plantlets were achieved. This has important implications for commercial production of ginger.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Multidisciplinary Sciences
Oezhan Simsek, Dicle Donmez, Mehmet Ali Sarida, Emine Acar, Yildiz Aka Kacar, Sevgi Paydas Kargi, Tolga Izgu
Summary: This study evaluated and compared different propagation systems for myrtle genotypes and found that the Plantform system was highly successful in micropropagation and rooting. This contributes to year-round propagation and conservation of genetic resources.
Article
Multidisciplinary Sciences
Grimar Abdiel Perez, Pumipat Tongyoo, Julapark Chunwongse, Hans de Jong, Anucha Wongpraneekul, Waraporn Sinsathapornpong, Paweena Chuenwarin
Summary: This study analyzed 112 Luffa acutangula accessions using 2834 SNPs to establish moderate genetic diversity and group them into six subpopulations. The STRUCTURE analysis revealed gene flow and low variation between subpopulations, with distinct fruit shapes and length characteristics in each subpopulation.
SCIENTIFIC REPORTS
(2021)
Article
Forestry
Maurizio Capuana, Werther Guidi Nissim, Joshua D. Klein
Summary: We describe a micropropagation protocol for three ecotypes of S. acmophylla, which showed that the best results for shoot proliferation were obtained by culturing the shoot explants on Woody Plant Medium containing benzyladenine. Separating the shoot tip from the underlying internodes further enhanced proliferation.
Article
Biotechnology & Applied Microbiology
Sandopu Sravan Kumar, Parvatam Giridhar
Summary: A novel and efficient in vitro micropropagation protocol was established for B. rubra shoot cultures without the use of plant growth regulators, achieving 100% elongation and rooting of microshoots. Seed germination was improved to 70% with urea treatment, and multiple shoot proliferation was initiated using nodal shoot segments from mature plants in pots. Multiple shoots were obtained with specific growth regulator combinations, and successful rooting was achieved in both MS medium and MS medium supplemented with activated charcoal.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Horticulture
Joao Martins, Aitana Ares, Joana Costa, Jorge Canhoto
Summary: The study identified endophytic communities on strawberry tree plant tissues in vitro, revealing significant differences in composition and diversity between in vitro and ex vitro samples. The microbiome of genotypes ex vitro showed higher diversity compared to in vitro plants. The genotype was found to be a key factor shaping the microbiota structure, with potential implications for micropropagation and breeding.
SCIENTIA HORTICULTURAE
(2022)
Article
Agronomy
Behzad Kaviani, Azam Barandan, Alicja Tymoszuk, Dariusz Kulus
Summary: Pears are economically important fruits that are grown worldwide. This study investigated the in vitro propagation of P. communis L. using different growth media and hormone combinations. The combination of 2 mg center dot L-1 BA and 1 mg center dot L-1 Kin in MS medium showed the highest shoot proliferation, while the longest shoots were obtained in WPM enriched with 1 mg center dot L-1 BA. The highest number of roots was obtained on MS medium augmented with 1 mg center dot L-1 IAA. The acclimatization of the produced plantlets resulted in a 90% survival rate.
Article
Horticulture
Mansur Hakan Erol, Dicle Donmez, Belgin Bicen, Ozhan Simsek, Yildiz Aka Kacar
Summary: In this study, different culture methods were compared for banana propagation and acclimatization. The results showed that bioreactor systems improved the efficiency of propagation and rooting, and the plants developed faster and healthier in the greenhouse stage. Higher stomatal activity and pigment accumulation were observed in bioreactor systems. No genetic variations were found during the subcultures. Bioractor systems are considered as an important alternative for mass production of bananas.
Article
Chemistry, Applied
Yogesh Kumar, Lochan Singh, Vijay Singh Sharanagat, Sarvanan Mani, Sourabh Kumar, Ankur Kumar
Summary: Drying spine gourd slices at different temperatures led to significant changes in its quality and nutrient content, with an increase in protein, fiber, total phenolic, and total flavonoid content as drying temperature increased, while reducing fat, carbohydrate, chlorophyll, ascorbic acid, and antioxidant activity. The mineral content of fresh spine gourd differed significantly from dried samples, and the temperature of drying had variable effects on the nutritional composition.
Article
Horticulture
Andrea Copetta, Paolo Mussano, Poonam Devi, Angelo Lanteri, Arianna Cassetti, Carlo Mascarello, Angela Bisio, Barbara Ruffoni
Summary: This study aimed to establish a successful protocol for fast shoot propagation, rooting, and in vivo acclimatization of Agastache aurantiaca 'Sunset Yellow' and A. mexicana 'Sangria' plants for edible flower production. Micropropagation with different levels of 6-benzylamino purine (BAP) was tested, and in vitro rooting trials were conducted using different methods. The best results were obtained with 2.22 and 3.33 μM BAP levels, and the temporary immersion system (TIS) led to earlier rooting and larger plants with longer roots. No anatomical differences were observed in leaves collected from different methods during the rooting stage. Plants from the TIS bioreactor showed the best acclimatization performance.
Article
Multidisciplinary Sciences
P. Gangadhara Rao, T. K. Behera, Ambika B. Gaikwad, A. D. Munshi, Arpita Srivastava, G. Boopalakrishnan, Vinod
Summary: In this study, a high-density linkage map of bitter gourd was constructed using F-2:3 mapping population and QTL analysis was performed for six major yield-contributing traits. The study identified QTLs with different genetic effects and provided valuable information for marker-assisted selection and molecular breeding in bitter gourd crop improvement.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yue Gao, Qin-Mei Wang, Qinxia An, Jianguo Cui, Yongbin Zhou, Xinyu Qi, Lijie Zhang, Lujia Li
Summary: An efficient micropropagation protocol from leaves and stems of L. ruthenicum was developed in this study, suggesting that sucrose supply might be a crucial regulator. Results of the study indicate that after acclimatization, different types of micropropagated plants showed greater epigenetic divergence.
Article
Multidisciplinary Sciences
Maqsood Ahmed Lakho, Mushtaque Ahmed Jatoi, Najamuddin Solangi, Adel Ahmed Abul-Soad, Muneer Ahmed Qazi, Gholamreza Abdi
Summary: A comprehensive study was conducted on in vitro multiplication and rooting of pineapple cv. 'Smooth Cayenne' by using different plant growth regulators. The highest shoot buds were obtained on the medium with 2.0 mg L-1 BA, while 1.0 mg L-1 IBA significantly increased the thickness, length, and number of white adventitious roots. When transplanted, plantlets showed highest survival rates in the medium containing only peat moss, followed by soil mixtures of bolhari and peat moss (1:1) and river silt alone. The optimized protocols in this study can be commercially applied for pineapple cultivation worldwide.
SCIENTIFIC REPORTS
(2023)
Article
Horticulture
Ieva Mezaka, Dace Kavina, Laura Kalane, Arta Kronberga
Summary: In this study, protocols for seed disinfection, in vitro multiplication and rooting, and ex vitro and field adaptation of the endangered medicinal plant Eryngium maritimum L. were developed. By optimizing the length and temperature of disinfectants, a lower contamination rate and high germination rate were achieved. Nitrogen content reduction and plant growth regulator addition enhanced axillary bud proliferation and rooting. Adjusting potting media and pot size affected survival and growth ex vitro. This research provides a suitable foundation for scaling up the production of E. maritimum through micropropagation.
Article
Biology
Merve Sifa Hane Kose, Muhammet Dogan, Gokhan Sadi
Summary: This study aimed to achieve multiple and rapid propagations of Staurogyne repens using tissue culture techniques. The shoot regeneration capabilities of the nodal explants were high due to the growth regulators used, with shoot regeneration frequencies ranging between 77.77% and 100.00% in different applications of BAP-IAA and KIN-IAA.
Article
Biology
Devendra Singh Rathore, Chirmaie Nagda, Bhavya Singh Shaktawat, Tanushree Kain, Chandrapal Singh Chouhan, Rakeshwar Purohit, Harish, Rama Kanwar Khangarot, Girima Nagda, Lalit Singh Jhala
Summary: The COVID-19 lockdown led to a substantial improvement in air quality in most countries, with a significant decrease in the concentration of major pollutants and a reduction in the air quality index. The largest decline was observed in Mumbai and Lucknow.
Review
Plant Sciences
Vishnu D. Rajput, Tatiana Minkina, Arpna Kumari, Harish, Vipin Kumar Singh, Krishan K. Verma, Saglara Mandzhieva, Svetlana Sushkova, Sudhakar Srivastava, Chetan Keswani
Summary: This study aims to comprehensively review the beneficial roles of NPs in plants under different abiotic stresses, especially heavy metals, salinity, and drought, providing deep insights about mechanisms of abiotic stress alleviation in plants under NP application.
Article
Environmental Sciences
Mohd Jahir Khan, Harish, Ankesh Ahirwar, Benoit Schoefs, Arivalagan Pugazhendhi, Sunita Varjani, Karthik Rajendran, Shashi Kant Bhatia, Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Vandana Vinayak
Summary: Wastewater can be effectively treated by growing microalgae in it, which not only reduces costs but also increases the production of value-added products. Ultrasonication pre-treatment of microalgal biomass allows for easier extraction of secondary metabolites at lower cost, especially with diatoms, which contain various valuable products.
ENVIRONMENTAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Kheta Ram, Ashok Kumar Patel, Sumitra Kumari Choudhary, N. S. Shekhawat
Summary: This study demonstrates the successful commercial propagation of Capparis decidua through technology-transfer from lab to land. By using mature nodal explants and a combination of plant growth regulators, efficient root development and multiplication of plants were achieved. The high survival rate of the plants in field conditions was also confirmed through experiments.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Microbiology
Pallavi Vyas, Harish
Summary: The continuous use of antibiotics has led to multidrug resistance among bacterial populations, posing a significant threat to public health. Anti-CRISPR proteins have been identified as potential therapeutic agents against drug-resistant bacteria.
MICROBIOLOGICAL RESEARCH
(2022)
Review
Microbiology
Kunal Seth, Geetanjali Kumawat, Pallavi Vyas, Harish
Summary: Light plays a crucial role in photosynthesis, photoperiodism, and photomorphogenesis. Algae use eyespots to perceive light signals and exhibit phototaxis. Mutational studies have identified genes involved in eyespot development and function, providing important insights into the light-sensing mechanism.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Environmental Sciences
Pallavi Saxena, Amit Kumar Gupta, Vinod Saharan, Harish
Summary: Boron nanoparticles have promising applications in biomedical, industrial, and environmental fields, but their potential impact on aquatic organisms is unclear. This study compared the effects of boron nitride nanoparticles and its bulk form on freshwater algae. The results showed that the nano form had a greater impact on the growth and biochemistry of the algae, and its toxicity was higher than that of the bulk form, with toxicity being concentration-dependent.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Microbiology
Mukesh Kumar, Sneha Sabu, Vishambhar Sangela, Mukesh Meena, Vishnu D. Rajput, Tatiana Minkina, Vandana Vinayak, Harish
Summary: The demand for nanoparticles is increasing, leading to a higher risk of their discharge into the environment. Although nanoparticles have been shown to be toxic to aquatic organisms, the causes of nanoparticle toxicity under environmental conditions are still unknown. Cyanobacteria are an ideal model system to study the impact of nanoparticle toxicity on aquatic biota, as they play a crucial role in ecological balance. Recent research suggests that shading effect, reactive oxygen species generation, membrane damage, and pigment disintegration are the main reasons for nanoparticle toxicity to cyanobacteria.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Jyoti Sharma, Iqra Mariam, Mukul Suresh Kareya, Pannaga Pavan Jutur, Monika Joshi, Harish, Amit Bhatnagar, Akhilesh K. Chaurasia, Subhasha Nigam
Summary: The efficiency of Chlorella sorokiniana in removing diclofenac sodium (DFS) from freshwater was assessed, and the metabolic response of the alga was studied to understand the metabolic pathways affected during DFS decontamination. The results showed that 91.51% of DFS could be removed within 9 days of algal treatment, and valuable bioresources such as chlorophyll, carotenoids, and lipids could be recovered from the spent biomass. DFS also affected enzyme activity and induced changes in metabolites in the microalgae. This study highlights microalgae as a green option for DFS removal and suggests that metabolomics analysis can provide insights into the stress-induced strategies of C. sorokiniana for simultaneous recovery of value-added products in the presence of DFS.
BIORESOURCE TECHNOLOGY
(2023)
Review
Chemistry, Medicinal
Anagha Nair, Ankesh Ahirwar, Shashikala Singh, Reeta Lodhi, Aishwarya Lodhi, Anshuman Rai, Dipak A. Jadhav, Harish, Sunita Varjani, Gurpreet Singh, Justine Marchand, Benoit Schoefs, Vandana Vinayak
Summary: Natural astaxanthin is richer than synthetic astaxanthin, but cultivating it in photobioreactors is expensive and downstream processing techniques for solubilization are not cost-effective. As a result, the cost of astaxanthin is high, leading pharmaceutical and nutraceutical companies to opt for synthetic astaxanthin. This review discusses the chemical character of astaxanthin, more cost-effective cultivating techniques, and its bioavailability. Moreover, the antioxidant properties of this microalgal product against various diseases are discussed, which make it a promising drug for reducing inflammation and its consequences.
Review
Green & Sustainable Science & Technology
Andleeb Zehra, Mukesh Meena, Dhanaji M. Jadhav, Prashant Swapnil, Harish
Summary: This review paper examines and provides an overview of the various conservation strategies and regulatory framework to protect endangered plants, particularly the Safed Musli. However, due to a lack of effective conservation, these plants are on the verge of extinction.
Article
Environmental Sciences
Mukesh Kumar, Kunal Seth, Sunita Choudhary, Geetanjali Kumawat, Subhasha Nigam, Garima Joshi, Vinod Saharan, Mukesh Meena, Amit Kumar Gupta, Harish
Summary: The extensive use of iron oxide nanoparticles in commercial and biomedical applications poses a potential ecotoxicological threat to aquatic ecosystems. This study aims to assess the cytotoxic effects of FeO NPs on cyanobacteria, which are primary producers in aquatic food chains. The results showed that FeO NPs had a greater impact on protein content and enzymatic activities compared to its bulk counterpart, indicating its higher toxicity. Microscopy techniques also revealed cellular damage caused by FeO NPs.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Nanoscience & Nanotechnology
Sunita Choudhary, Vishambhar Sangela, Pallavi Saxena, Vinod Saharan, Arivalagan Pugazhendhi, Harish
Summary: The use of algae for synthesizing AgNPs offers advantages in terms of low cost, absence of hazardous chemical reagents, and ease of maintenance and mass multiplication.
INTERNATIONAL NANO LETTERS
(2023)
Review
Plant Sciences
Prashant Swapnil, Mukesh Meena, Sandeep Kumar Singh, Umesh Praveen Dhuldhaj, Harish, Avinash Marwal
Summary: Carotenoids are important molecules involved in various biological processes in plants and humans, acting as essential compounds for photosynthetic tissues and non-photosynthetic tissues. They play a crucial role in light absorption and photoprotection, and contribute to skin health and protection against diseases in humans.
CURRENT PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ravneet Chug, Shruti Mathur, S. L. Kothari, Harish, Vinod Singh Gour
Summary: The bacterium Pseudomonas aeruginosa produces extracellular polymeric substances (EPS) that have the potential to sequester heavy metal ions in water bodies contaminated with Cr (VI) and Ni (II). The study investigated the effect of time, pH, and temperature on EPS production and its ability to remove Cr (VI) and Ni (II) ions from aqueous systems. Results showed that specific functional groups of EPS were involved in binding with Cr (VI) and Ni (II) ions, demonstrating the potential for scaling up EPS production using a bioreactor for efficient treatment of water polluted with these heavy metals.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)