Article
Pharmacology & Pharmacy
John D. Imig, Md Abdul Hye Khan, Anna Stavniichuk, Wojciech K. Jankiewicz, Samaneh Goorani, Michael M. Yeboah, Ashraf El-Meanawy
Summary: The incidence of ureter obstruction is increasing and recovery from this kidney injury can lead to long-term consequences such as an increased risk for salt-sensitive hypertension. A study using a RUUO model found that mice with unilateral ureteral obstruction had elevated blood pressure and decreased sodium excretion, along with renal tubular injury, inflammation, decreased vascular density, and renal fibrosis. These findings highlight the importance of addressing ureter obstruction and its potential impact on sodium excretion and hypertension.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Immunology
Kirti Hira, Pravesh Sharma, Ashutosh Mahale, Onkar Prakash Kulkarni, A. Sajeli Begum
Summary: The study identified natural compounds that can alleviate renal injury by reducing inflammation and fibrosis markers. These compounds showed significant effects in decreasing plasma IL-1β levels, regulating mRNA expression levels of inflammatory and renal injury markers, and improving histological damage. The compounds also demonstrated protective and antiapoptotic effects in vitro, as well as significant anti-fibrotic activity. Therefore, these proline-based cyclic dipeptides are recommended as potential drug leads for treating renal injury.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Urology & Nephrology
Chao Gao, Long Zhang, Enuo Chen, Wenzheng Zhang
Summary: This study demonstrates that Aqp2(+) B1B2(+) cells behave as adult progenitor cells to maintain and repair DCT2/CNT1/CNT2/CD segments.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Julia Stabinska, Aruna Singh, Nora M. Haney, Yuguo Li, Farzad Sedaghat, Max Kates, Michael T. McMahon
Summary: This study investigates the potential of DCE MR CEST urography for assessing renal function in mice with unilateral ureter obstruction (UUO) by simultaneous pH and renal uptake/clearance measurements. The results show that DCE-MR-CEST urography can detect changes in renal uptake/excretion and pH homeostasis and distinguish between obstructed and unobstructed kidney as early as 1 day after UUO.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Biophysics
Feng Wang, Tadashi Otsuka, Keiko Takahashi, Chikage Narui, Daniel C. Colvin, Raymond C. Harris, Takamune Takahashi, John C. Gore
Summary: Utilizing quantitative MRI relaxometry, particularly spin-lock relaxation dispersion analysis, shows promise in detecting and evaluating tubular dilation and fibrosis in a mouse model of UUO. Changes in relaxation rates provide valuable information on tubular dilation and fibrosis progression, with specific parameters like S-rho showing high correlations with renal fibrosis. This technique offers additional specific insights into fibrosis assessment in kidney disease beyond traditional histological methods.
NMR IN BIOMEDICINE
(2021)
Article
Medicine, General & Internal
Ercan Yuvanc, Devrim Tuglu, Tunc Ozan, Ucler Kisa, Mahi Balci, Ertan Batislam, Erdal Yilmaz
Summary: The experimental study demonstrated that pheniramine maleate (PM) and zofenopril can reduce oxidation and tissue damage caused by unilateral partial obstruction in the kidney.
ARCHIVES OF MEDICAL SCIENCE
(2021)
Article
Obstetrics & Gynecology
Phillip A. Romanski, Pietro Bortoletto, Samantha M. Pfeifer
Summary: This article discusses three cases of unilateral Mullerian obstructions in adolescent female patients, highlighting the potential for unusual variations even among well-known Mullerian anomalies. The importance of correct diagnostic tools and individualized treatment plans for successful management of these conditions is emphasized.
JOURNAL OF PEDIATRIC AND ADOLESCENT GYNECOLOGY
(2021)
Editorial Material
Rheumatology
Taro Horino, Satoshi Inotani, Hideki Nakajima, Hiroshi Ohnishi, Masahiro Komori, Osamu Ichii, Yoshio Terada
LANCET RHEUMATOLOGY
(2023)
Article
Cell Biology
Marina Hosotani, Osamu Ichii, Takashi Namba, Md Abdul Masum, Teppei Nakamura, Yasuhiro Hasegawa, Takafumi Watanabe, Yasuhiro Kon
Summary: In this study, we investigated the expression of hedgehog signaling-related components in the murine oviductal infundibulum and found that the homeostasis of the infundibulum epithelium was disturbed in MRL/lpr mice. The motile cilia of ciliated epithelial cells in the infundibulum harbored Hh signaling pathway-related molecules. However, these molecules were significantly downregulated in MRL/lpr mice at 6 months of age. Pax8 and Foxj1 expression levels were positively correlated with those of Ptch1, Smo, and Gli2. Overall, Hh signaling is involved in the regulation of ciliated epithelium homeostasis in the infundibulum and interacts with inflammation, leading to disturbances in epithelial homeostasis and reproductive function.
CELL AND TISSUE RESEARCH
(2023)
Review
Cell Biology
Hayder M. Al-kuraishy, Ali I. Al-Gareeb, Yaser Hosny Ali Elewa, Mahmoud Hosny Zahran, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha
Summary: Parkinson's disease (PD) is a degenerative brain disorder characterized by the loss of dopamine neurons in the substantia nigra and the accumulation of Lewy bodies and α-synuclein in the brain. Patients with PD often have deficiencies in vitamins such as folate, vitamin B6, and vitamin B12, leading to increased levels of homocysteine in the bloodstream, which may contribute to the development and progression of PD through oxidative and inflammatory pathways. Hyperhomocysteinemia is implicated in neurodegenerative disorders and triggers PD through various mechanisms, including oxidative stress, mitochondrial dysfunction, apoptosis, and endothelial dysfunction. Inflammatory signaling pathways like NF-kappa B and NLRP3 inflammasome are also involved in the pathogenesis of PD. In conclusion, hyperhomocysteinemia plays a role in the neuropathology of PD by directly inducing degeneration of dopamine neurons or indirectly activating inflammatory signaling pathways.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Marina Hosotani, Osamu Ichii, Takafumi Watanabe, Yasuhiro Kon
Summary: Oocyte transportation in the oviduct is influenced by the interaction between ciliated epithelial cells and cumulus cells. In this study, we investigated the effect of cumulus-oocyte complex (COC) quality on the transportation property of COCs by comparing the transportation velocity of COCs (TVC) in different combinations of infundibula and COCs collected from various mice. Our results showed that the TVC was lower in aged MRL strains compared to aged C57BL/6N mice, indicating that aging affects COCs transportation in MRL strains. Moreover, the TVC was the lowest in aged MRL/MpJ-Fas (lpr/lpr) mice, while it accelerated when the infundibulum or COCs were collected from other strains, suggesting that the properties of COCs also play a role in determining their transportation property.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Yaser Hosny Ali Elewa, Mahmoud M. Abd Elwakil, Hideyoshi Harashima, Sherif Kh A. Mohamed, Mahmoud Hosny Zahran
Summary: By comparing the changes in the lungs and mediastinal fat-associated lymphoid clusters (MFALCs) between patients with metastatic lung melanoma (MLM) and healthy individuals, it was found that MLM patients had a significant increase in immune cell populations in MFALCs, which may be associated with the progression of MLM. Therefore, targeting immune cells in MFALCs could be a promising therapeutic strategy for cancer treatment.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Multidisciplinary Sciences
Mei Sugawara-Suda, Keitaro Morishita, Osamu Ichii, Takashi Namba, Keisuke Aoshima, Yumiko Kagawa, Sangho Kim, Kenji Hosoya, Nozomu Yokoyama, Noboru Sasaki, Kensuke Nakamura, Jumpei Yamazaki, Mitsuyoshi Takiguchi
Summary: In this study, splenectomy was performed as an alternative therapy for refractory PIMA in dogs, and gene expression levels in the spleens and serum of dogs with PIMA were analyzed. Transcriptome analysis revealed differential gene expression in the spleens of dogs with PIMA, with up-regulation of genes such as S100A12, S100A8, and S100A9. Immunohistochemistry confirmed higher levels of S100A8/A9 protein expression in dogs with PIMA. Proteome analysis identified differentially expressed proteins in the serum samples before and after splenectomy, with up-regulation of proteins in the pre-splenectomy samples. Pathway analysis revealed the involvement of the lectin pathway of complement activation in the pre-splenectomy samples. These findings provide deeper insights into the pathology and mechanisms of splenectomy for PIMA.
Article
Microbiology
Takuya Yagisawa, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Shun Ando, Osamu Ichii, Hironobu Murakami, Osamu Matsushita, Seiji Katagiri
Summary: We examined the uterine microbiota associated with low fertility in dairy cows and found two new insights into their relevance to fertility. The deterioration in reproductive performance due to low fertility leads to significant economic losses on dairy farms, making the uterine microbiota an interesting area for research. Our study analyzed the uterine microbiota in dairy cows and found that it can vary depending on farm management practices, indicating its potential as a measure for low fertility.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Misato Masugi, Osamu Ichii, Yuki Otani, Takashi Namba, Yasuhiro Kon
Summary: Immune cells play a crucial role in skeletal muscle repair, and systemic autoimmune diseases can impair muscle regeneration. This study found that, regardless of the effects of autoimmune diseases, injured skeletal muscles caused by minor trauma can normally regenerate, although lymphocyte infiltrations were more severe in mice with autoimmune diseases.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Sahar F. Mahmoud, Yaser H. Elewa, Ahmed G. Nomir, Ahmed M. Rashwan, Ahmed E. Noreldin
Summary: The study aimed to investigate the influence of proliferation and apoptosis in different compartments of the kidney on the developmental function of the protein calbindin-D28k. The results showed that calbindin localization was inversely related to PCNA and ssDNA in the nephron compartments, indicating their role in bone-building and muscle contraction efficiency during animal development.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Veterinary Sciences
Hikari Seo, Shiro Murata, Osamu Ichii, Takashi Namba, Shwe Yee Win, Takumi Sato, Eiji Oishi, Akira Taneno, Naoya Maekawa, Tomohiro Okagawa, Satoru Konnai, Kazuhiko Ohashi
Summary: In this study, RNAscope-based in situ hybridization (ISH) was used to analyze gene expression in the poultry red mite (PRM). The results showed that Dg-CatD-1 mRNA was mainly expressed in the digestive tract, while Dg-Cystatin mRNA was expressed in the ovaries and digestive tract. RNAscope ISH is an effective method for investigating gene expression and characterizing target genes in PRM.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2023)
Article
Veterinary Sciences
Ayana Noguchi, Natsume Kurahara, Osamu Yamato, Osamu Ichii, Akira Yabuki
Summary: This study investigated the lectin-binding patterns in normal feline kidneys and established a feline-specific lectin-binding pattern. The findings provide a foundation for future glycopathological studies of feline chronic kidney disease.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yuka Katayama, Osamu Ichii, Teppei Nakamura, Keita Yanase, Masaya Hiraishi, Takashi Namba, Yuki Otani, Teppei Ikeda, Erika Tsuji, Natsuko Tsuzuki, Ken Kobayashi, Yasuhiro Kon, Takanori Nishimura
Summary: This study demonstrated the formation of connective tissue in dairy cows following subcutaneous implantation of acrylic resin-based implants created using 3D printing technology. The results provide insights into the tissue remodeling process related to foreign body reactions.
Article
Agriculture, Dairy & Animal Science
Keigo Kosenda, Osamu Ichii, Yusuke Yamashita, Hiromichi Ohtsuka, Shigeo Fukuda, Yasuhiro Kon
Summary: The conjunctiva of cows exhibits immune cell aggregation, but calves have a weaker immune function compared to adult cows. This suggests that cows have a conjunctival local immune system similar to that of other animals, but the immune function is less developed in calves.