Article
Food Science & Technology
Cecile Miot-Sertier, Margot Paulin, Lucie Dutilh, Patricia Ballestra, Warren Albertin, Isabelle Masneuf-Pomarede, Joana Coulon, Virginie Moine, Amelie Vallet-Courbin, Julie Maupeu, Marguerite Dols-Lafargue
Summary: Chitosan is an active polysaccharide that has been developed for eliminating spoilage yeast in winemaking. The antimicrobial efficiency of chitosan on various wine microorganisms has been investigated, showing that most species are affected to some extent. The study also highlights that the efficiency of chitosan varies depending on wine parameters and stages of winemaking. Chitosan treatment is not suitable for limiting microbial pressure in musts and has limited impact on certain microorganisms such as Saccharomyces cerevisiae and acetic acid bacteria. Different types of chitosan have different antimicrobial properties, with lower molecular weight chitosan being more effective.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fei Shen, Qianqian Wang, Sami Ullah, Ya Pan, Minjie Zhao, Jing Wang, Ming Chen, Fengqin Feng, Hao Zhong
Summary: This study investigated the peristalsis-promoting potential of Ligilactobacillus acidipiscis YJ5 and its underlying molecular mechanism in relieving constipation. The results showed that L. acidipiscis YJ5 intervention increased intestinal peristalsis in zebrafish and mouse models, reduced colonic aquaporin expression, repaired intestinal morphological damage, enhanced the colonic mucosal barrier, and reversed the gut microbiota structure. The positive effects of L. acidipiscis YJ5 were attributed to its metabolites such as malic acid and heliangin.
Review
Chemistry, Applied
Shuxun Liu, Ying Lou, Yixian Li, Yan Zhao, Oskar Laaksonen, Ping Li, Jiaojiao Zhang, Maurizio Battino, Baoru Yang, Qing Gu
Summary: Wine is a complex mixture with different chemical components, and aroma is a crucial indicator of its sensory characteristics. The metabolic activities of diverse microbes play a significant role in the aroma of wine, and understanding their impact is essential for producing premium quality wine.
Article
Biotechnology & Applied Microbiology
Tomoyuki Shimazu, Mai Suzuki, Ryo Takasaki, Asuka Besshi, Yuna Suzuki, Yoichiro Iwakura
Summary: This study investigated the relationship between lactic acid bacteria (LAB) and innate immune receptors. The researchers found that C-type lectin receptors (CLRs) are involved in the immune-stimulating function of LAB. The interaction between LAB and CLRs can reduce the levels of certain cytokines and is only observed in heat-killed LAB. These findings contribute to a better understanding of the immunomodulatory effects of LAB.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Review
Engineering, Multidisciplinary
Jia-Rong Zhang, Ying-Ying Ge, Pin-He Liu, Ding-Tao Wu, Hong-Yan Liu, Hua-Bin Li, Harold Corke, Ren-You Gan
Summary: Riboflavin is an essential micronutrient for humans, with potential roles in disease prevention and treatment. Microbial fermentation can enhance riboflavin levels in foods, offering beneficial properties for human health.
Article
Food Science & Technology
Jae-Seong Kim, Seong-Eun Park, Eun-Ju Kim, Seung-Ho Seo, Hong-Seok Son
Summary: In this study, green coffee beans were fermented using different yeast and lactic acid bacteria starters, and the effects on metabolite profiles were analyzed. There was a clear separation between the groups fermented with LAB and yeast, with different associated metabolites. Chlorogenic acid levels were higher in LAB strains, particularly lower in Candida parapsilosis and Pichia guilliermondii. Caffeine levels were highest in Pediococcus pentosaceus and lowest in C. parapsilosis. This study provides comprehensive information on the metabolic changes after green coffee bean fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Andrea Laukova, Martin Tomaska, Maria Joao Fraqueza, Renata Szaboova, Eva Bino, Jana Scerbova, Monika Pogany Simonova, Emilia Dvoroznakova
Summary: This study demonstrates the antimicrobial potential of Lactiplantibacillus plantarum LP17L/1 isolated from stored ewe's milk lump cheese, showing active bacteriocin against Staphylococcus and Listeria. It also exhibits safety and survival capability in mice, making it a promising additive for Slovak local dairy products.
Review
Food Science & Technology
Alexander J. Taylor, Eduardo Cardenas-Torres, Michael J. Miller, Sihai Dave Zhao, Nicki J. Engeseth
Summary: This study investigates spontaneous cacao fermentations (SCFs) in the production of chocolate, aiming to identify SCF microbes, their interrelationships, and other key fermentation parameters. The analysis of over 1700 microbes across 60 articles reveals the major gaps in knowledge about the cacao microbiome. By understanding the cacao microbiome, researchers can optimize fermentation by identifying key microbes and fermentation parameters.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Review
Immunology
Robert Clancy Am
Summary: The concept of a common mucosal immune system (CMS) has been neglected for 50 years, leading to a lack of understanding of its significance in immune protection at mucosal sites and its potential effects on vaccination-induced protection against airway infection. The failure of current SARS-CoV-2 vaccination to meet expectations has raised concerns and prompted a review of public health and medical decisions. This review highlights the role of CMS in immune protection, discusses the limitations of systemic and mucosal vaccination, and showcases the potential of optimizing immune protection using respiratory microbiome isolates.
Article
Cell Biology
Fei Teng, Roser Tacho-Pinot, Biin Sung, Donna L. Farber, Stefan Worgall, Hamida Hammad, Bart N. Lambrecht, Matthew R. Hepworth, Gregory F. Sonnenberg
Summary: This study demonstrates that LTi-like ILC3s expressing MHC class II are enriched in lung-draining lymph nodes and play a critical role in restricting pro-inflammatory T cell responses in the airway, leading to reduced allergic airway inflammation and associated cellular responses.
Review
Microbiology
Bakhtawar Shafique, Muhammad Modassar Ali Nawaz Ranjha, Mian Anjum Murtaza, Noman Walayat, Asad Nawaz, Waseem Khalid, Shahid Mahmood, Muhammad Nadeem, Muhammad Faisal Manzoor, Kashif Ameer, Rana Muhammad Aadil, Salam A. Ibrahim
Summary: This article discusses the application of bacteriocins, peptides or proteins produced by bacteria, in food. However, the activity of bacteriocins in food matrices is influenced by various factors. Fortunately, nanotechnology-based encapsulation has emerged as a promising solution.
Article
Oncology
Wenyong Huang, Dongmei Ye, Wenjing He, Xiaoshun He, Xiaomin Shi, Yifang Gao
Summary: The study found that MAIT cells in patients with HCC had lower levels in both peripheral blood and liver compared to healthy controls, with reduced IFN-γ production. While peripheral MAIT cells showed upregulation of HLA-DR and PD-1, intrasinusoidal MAIT cells did not exhibit significant differences. Additionally, MAIT cells were significantly enriched in the livers of HCC patients, showing high levels of activation and exhaustion markers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Agriculture, Dairy & Animal Science
Ailian Lin, Xiaoxi Yan, Hongyu Wang, Yong Su, Weiyun Zhu
Summary: Lactic acid bacteria-fermented formula milk has positive effects on the growth performance, intestinal immunity, and microbiota composition of weaned piglets. It improves the average daily gain and feed intake, increases the count of ileal goblet cells and villus-crypt ratio, and enhances the levels of sIgA and lactate. It also regulates the intestinal immune network and secretory function of goblet cells, and leads to the enrichment of lactate-producing and short-chain fatty acid-producing bacteria in the intestine.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Noriko Kawamoto-Miyamoto, Hiroshi Hosoda, Kazuyuki Miyoshi, Koji Nomoto
Summary: Lactic acid bacteria (LAB) cultured on food grade medium showed smaller cell size and higher yield, and exhibited a stronger ability to induce IL-12(p40) production. Furthermore, the concentration of glutamate in the medium influenced the cell size, yield, and the ability to induce IL-12 production. Therefore, regulating the concentration of glutamate is crucial for efficient culturing of functional LAB.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Review
Food Science & Technology
Svetoslav Dimitrov Todorov, Igor Popov, Richard Weeks, Michael Leonidas Chikindas
Summary: This review critically revisits various research approaches for the application of beneficial organisms and bacteriocins in the food industry. Most studies propose potential applications of these microorganisms and bacteriocins as biopreservatives, but few have evaluated the possible adverse effects of bacteriocins.
Review
Microbiology
Julio Villena, Chang Li, Maria Guadalupe Vizoso-Pinto, Jacinto Sacur, Linzhu Ren, Haruki Kitazawa
Summary: This study highlights the potential of LAB-based vaccines in fighting against COVID-19 and emphasizes the important role of Lactiplantibacillus plantarum in improving mucosal antiviral defenses, expressing heterologous proteins, and successfully expressing viral antigens.
Review
Microbiology
A. K. M. Humayun Kober, Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Julio Villena, Haruki Kitazawa
Summary: In the past decade, the use of probiotics as feed supplements in animal production has significantly increased due to the ban on antibiotic growth promoters. Probiotics have been suggested to improve the health, immunity, growth performance, nutritional digestibility, and intestinal microbial balance of livestock. Furthermore, they can help balance the beneficial microbial population and turnover in animals by stimulating the host immune response and excluding potentially pathogenic bacteria.
Article
Biochemistry & Molecular Biology
Leonardo Albarracin, Ramiro Ortiz Moyano, Juan Martin Vargas, Bruno G. N. Andrade, Juan Cortez Zamar, Stefania Dentice Maidana, Kohtaro Fukuyama, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: In this study, the virulence and immune response of two hypermucoviscous carbapenem-resistant Klebsiella pneumoniae strains were evaluated. The results showed that LABACER01 had higher pathogenicity and caused more severe lung tissue damage compared to LABACER27. Genomic analysis revealed the presence of virulence-related genes and adhesion genes in both strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mikado Tomokiyo, Fernanda Raya Tonetti, Hikari Yamamuro, Ryoko Shibata, Kohtaro Fukuyama, Nadia Gobbato, Leonardo Albarracin, Muhammad Shahid Riaz Rajoka, A. K. M. Humayun Kober, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa
Summary: The study found that postimmunobiotics derived from Lactobacillus gasseri can alter the antiviral immune response of alveolar macrophages and have a protective effect against lung inflammatory damage caused by TLR3 activation.
Article
Microbiology
Stefania Dentice Maidana, Ramiro Ortiz Moyano, Juan Martin Vargas, Kohtaro Fukuyama, Shoichiro Kurata, Vyacheslav Melnikov, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: In this study, we evaluated the immunomodulatory effect of Corynebacterium pseudodiphtheriticum 090104 strain against the respiratory infection induced by hypermucoviscous carbapenemase-producing Klebsiella pneumoniae strains. The results showed that pre-treatment with 090104 strain significantly reduced lung bacterial cell counts and tissue damage, and regulated the respiratory innate immune response. This study advances the positioning of 090104 strain as a next-generation probiotic for the respiratory tract and suggests its potential as a non-antibiotic therapeutical approach.
Article
Food Science & Technology
Masahiko Suzuki, Leonardo Albarracin, Yuji Tsujikawa, Kohtaro Fukuyama, Iwao Sakane, Julio Villena, Haruki Kitazawa
Summary: This study characterized the potential probiotic properties of Lactiplantibacillus plantarum LOC1, including its immunomodulatory effects on innate immunity. The study demonstrated that LOC1 strain can modulate inflammatory response in macrophages and enhance their protective effects mediated by the stimulation of the Th1 response. Comparative genomics analysis revealed that LOC1 possesses genes related to adhesion and immunomodulation. These findings contribute to the development of immune-related functional foods containing LOC1.
Article
Agriculture, Dairy & Animal Science
Kasumi Suzuki, Hiroki Shinkai, Gou Yoshioka, Toshimi Matsumoto, Takato Takenouchi, Junji Tanaka, Masanori Shimizu, Haruki Kitazawa, Hirohide Uenishi
Summary: This study found that DNA markers in immune-genes can be used to reduce respiratory diseases caused by bacterial pathogens in pig farming.
Article
Microbiology
Ayelen Baillo, Julio Villena, Leonardo Albarracin, Mikado Tomokiyo, Mariano Elean, Kohtaro Fukuyama, Sandra Quilodran-Vega, Silvina Fadda, Haruki Kitazawa
Summary: The immunobiotic potential of L. plantarum CRL681 and CRL1506 was studied in this research. The results showed that both strains could induce the expression of IL-6 and IL-8 in intestinal epithelial cells and enhance the levels of IFN-gamma and TNF-alpha in mice. In addition, these strains could modulate the expression of inflammatory cytokines and chemokines, reduce the pathogen counts, and prevent its spread to other organs. L. plantarum CRL1506 was found to be more efficient in modulating mucosal immunity than CRL681.
Review
Microbiology
A. K. M. Humayun Kober, Sudeb Saha, Md. Aminul Islam, Muhammad Shahid Riaz Rajoka, Kohtaro Fukuyama, Hisashi Aso, Julio Villena, Haruki Kitazawa
Summary: This review article provides an overview of recent advances in the use of probiotics for the prevention and treatment of bovine mastitis. Research has shown that probiotics can suppress mammary pathogens through competitive exclusion or the production of antimicrobial compounds. Additionally, evidence suggests that probiotics can modulate the immune response of mammary glands to inflammation. These findings indicate that probiotics can be used as a preventive strategy for managing mastitis and antimicrobial resistance, while also improving milk production and animal and human health.
Article
Biochemistry & Molecular Biology
Stefania Dentice Maidana, Mariano Elean, Kohtaro Fukuyama, Yoshiya Imamura, Leonardo Albarracin, Sudeb Saha, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: An increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) was observed in hospitals in Norwest Argentina. In this study, it was found that the ST25 strains of K. pneumoniae can invade human intestinal epithelial cells and induce moderate inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ramiro Ortiz Moyano, Fernanda Raya Tonetti, Kohtaro Fukuyama, Mariano Elean, Mikado Tomokiyo, Yoshihito Suda, Vyacheslav Melnikov, Haruki Kitazawa, Julio Villena
Summary: Cp and BLPs can stimulate alveolar macrophages and enhance the humoral immune response induced by the commercial pneumococcal vaccine. Mice immunized with Cp + PCV or BLPs + PCV showed higher levels of specific antibodies and lower levels of pneumococcal cell counts and lung damage compared to the control group. These findings demonstrate the potential of Cp and BLPs as adjuvants to enhance the immune response against respiratory pathogens.
Article
Microbiology
Stefania Dentice Maidana, Yoshiya Imamura, Mariano Elean, Leonardo Albarracin, Keita Nishiyama, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, its effectiveness against Gram-negative bacterial infections has not been evaluated. This study aimed to assess whether Lcb. rhamnosus CRL1505 could beneficially regulate the respiratory innate immune response and improve resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25).
Article
Biochemistry & Molecular Biology
Mariano Elean, Fernanda Raya Tonetti, Kohtaro Fukuyama, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Nadia Gobbato, Keita Nishiyama, Julio Villena, Haruki Kitazawa
Summary: In this study, nasally administered L. salivarius FFIG58 was found to modulate the innate immune response in the respiratory tract and provide long-term protection against Streptococcus pneumoniae infection. The strain enhanced the production of various immune factors and protected mice from secondary infection for up to 30 days.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariano Elean, Leonardo Albarracin, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Summary: A comprehensive genomic analysis of L. delbrueckii strains was conducted, focusing on the proteolytic system and the key enzyme, cell envelope-associated proteinase (CEP). The study compared amino acid sequences and protein structures of CEPs, as well as analyzed syntenic and genomic variations among different strains. Investigation of CEP promoter region and regulatory elements provided insights into the control of proteolytic activity in lactobacilli. This study contributes to our understanding of proteolytic activity and potential applications of L. delbrueckii in the dairy industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Sudeb Saha, Kohtaro Fukuyama, Marina Debnath, Fu Namai, Keita Nishiyama, Haruki Kitazawa
Summary: This review focuses on the effect of probiotics on improving the quality of meat from small ruminants. The findings of the reviewed articles show inconsistent results due to the use of different probiotic strains, dosage rates, experiment duration, nutrition, breed, age, and animal health status. Future research should emphasize specific strains, optimal dose and days of administration, route, and mechanisms for the specific probiotic strains to host.
