Article
Fisheries
Sheng-Wei Luo, Ning-Xia Xiong, Zi-Ye Luo, Lan-Fen Fan, Kai-Kun Luo, Zhuang-Wen Mao, Shao-Jun Liu, Chang Wu, Fang-Zhou Hu, Shi Wang, Ming Wen
Summary: NK-lysin in hybrid fish exhibits cytotoxicity and immune defense against bacterial infection, regulating inflammatory responses and bacterial loads in the fish after infection.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Hao Ma, Yueh-Chiang Han, Yniv Palti, Guangtu Gao, Sixin Liu, Debra E. Palmquist, Gregory D. Wiens, Brian S. Shepherd
Summary: Nk-lysin (Nkl) is an antimicrobial peptide (AMP) found in mammals, and in this study, six Nkl genes were identified and characterized in rainbow trout, showing tissue-specific expression patterns and potential role in innate immunity.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Tyler E. Boggs, Jessica S. Friedman, Joshua B. Gross
Summary: This study investigates the adaptive traits of Astyanax mexicanus, a freshwater fish, in response to hypoxia in cave environments. The results show that cavefish have higher concentrations of hemoglobin in their blood and larger erythrocyte size compared to surface fish. These findings suggest that cavefish have adapted to hypoxia through modulation of hemoglobin production and erythrocyte size.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Xinyu Li, Heng Chi, Roy Ambli Dalmo, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: In this study, recombinant proteins rPoHep2 and rPoNKL were obtained from flounder and found to possess antimicrobial and antiviral activities, as well as immune regulatory functions. These proteins showed no cytotoxicity to mammalian and fish cell lines and could potentially be used as alternatives to traditional antibiotics in aquaculture.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Yulema Valero, Carmen Gonzalez-Fernandez, Constanza Cardenas, Fanny Guzman, Rosa Leon, Alberto Cuesta
Summary: Antimicrobial peptides (AMP) are small molecules with potential antibacterial, antiviral, and antifungal effects, with NK-lysin being a crucial molecule in both innate immunity and cell-mediated cytotoxic responses. Studies have shown that NK-lysin derived peptides (NKLPs) have direct antiviral activity against nervous necrosis virus (NNV) in vitro, making them a potential prophylactic treatment for European sea bass. More research is needed to understand the mechanisms of action and applicability of NKLPs in the aquaculture industry.
ANTIVIRAL RESEARCH
(2021)
Article
Fisheries
Chang-biao Wang, Xue Yan, Guang-hua Wang, Wen-qing Liu, Yue Wang, Dong-fang Hao, Min Zhang
Summary: In this study, a sevenband grouper NK-Lysin peptide, NKHs27, was identified and studied. The results showed that NKHs27 had antibacterial activity against both Gram-negative and Gram-positive bacteria and showed a synergistic effect when combined with rifampicin or erythromycin. Furthermore, NKHs27 enhanced the immune response and leukocyte proliferation and reduced pathogen dissemination and replication in vivo. These findings suggest that NKHs27 has potential applications in aquaculture as a broad-spectrum antibacterial peptide.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Maria Andrea Hernandez-Castaneda, Marilyne Lavergne, Pierina Casanova, Bryan Nydegger, Carla Merten, Bibin Yesodha Subramanian, Patricia Matthey, Nils Lannes, Pierre-Yves Mantel, Michael Walch
Summary: Malaria is a serious health problem in developing countries, and Plasmodium parasites deplete cholesterol in red blood cells making their membranes vulnerable, but it is the exposure of phosphatidylserine that triggers resistance to eukaryotic pore-forming proteins in parasitized cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Hematology
Amin A. Alamin
Summary: New research is shedding light on the roles played by red blood cells (RBCs) in hemostasis, particularly blood clotting. Previous studies mainly focused on quantitative measures of RBCs, but recent findings are uncovering the various functions of RBCs and their interactions with platelets in clot formation. This renewed interest in RBCs has led to the suggestion that they could be potential targets for treating hemostatic disturbances.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Engineering, Biomedical
Paige N. Smith, Leran Mao, Kaustubh Sinha, Alan J. Russell
Summary: Organophosphate nerve agents (OPNAs) are extremely toxic chemical warfare agents. Access to prophylactic measures is crucial due to their toxicity. Researchers have developed an enhanced delivery system by engineering butyrylcholinesterase (BChE) to the surface of red blood cells (RBCs).
ACTA BIOMATERIALIA
(2021)
Article
Fisheries
Dong-fang Hao, Guang-hua Wang, Ning-qiu Li, Hong-mei Liu, Chang-biao Wang, Wen-qing Liu, Xue Yan, Min Zhang
Summary: NK-lysin homologue SsNKL1 was characterized in black rockfish, which showed direct antibacterial activity and enhanced the bactericidal process of erythromycin. In vivo studies demonstrated that SsNKLP27 reduced pathogen transmission and replication in tissues, while in vitro analysis revealed its ability to enhance macrophages' respiratory burst ability and regulate the expression of immune-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Medicine, Research & Experimental
Nicola Conran, Stephen H. Embury
Summary: Sickle cell anemia is a hereditary hemoglobinopathy characterized by abnormal hemoglobin polymerization that triggers pathological mechanisms leading to various severe complications. Recent research has focused on the role of leukocytes in vaso-occlusive processes and the importance of adhesion by different cell types, including endothelial cells, platelets, leukocytes, and red blood cells.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Hematology
Laura Hertz, Daniel Flormann, Lutz Birnbaumer, Christian Wagner, Matthias W. Laschke, Lars Kaestner
Summary: The protein TRPC6 is present in mouse red blood cells but absent in human red blood cells. Transfusion experiments showed that TRPC6 could be restored in mice within 10 days. Mechanical stimulation experiments suggested that protein transfer to red blood cells can occur in the absence of translational machinery. This protein transfer mechanism has potential therapeutic applications for diseases involving red blood cells.
Review
Chemistry, Multidisciplinary
Antony Vincy, Sarmistha Mazumder, Indranil Banerjee, Kuo Chu Hwang, Raviraj Vankayala
Summary: Red Blood Cells (RBCs)-derived particles are potential drug carriers or camouflage materials due to their innate biocompatibility. However, their translation from lab to clinic applications is hindered.
FRONTIERS IN CHEMISTRY
(2022)
Article
Environmental Sciences
Burcu Yuksel
Summary: The study found that occupational exposure in dental prosthesis laboratories can have cytotoxic effects on erythrocyte cells, with statistically significant morphological anomalies observed in both the experimental group and the control group. It was concluded that strict compliance with occupational health precautions is necessary for DPL employees.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Microscopy
Ian M. Davis
Summary: This article addresses the insufficient coverage of Antoni van Leeuwenhoek's role in observing and describing red blood cells and hemocytes in the existing cell biology and hematology literature. By examining Leeuwenhoek's letters and observations, the article highlights his extensive descriptions of both red blood cells and arthropod hemocytes, as well as his experimentation in different chemical and physical environments.
Article
Fisheries
Alejandro Romero, Beatriz Novoa, Antonio Figueras
Summary: This study identified most of the cathepsin family members in the bivalve Mytilus galloprovincialis and found a high diversification of cathepsin D, L, and F. Transcriptomic analysis revealed the coordinated activity of these proteins during larval development and their important role in the immune response of bivalves.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Raquel Rios-Castro, Raquel Aranguren, Alejandro Romero, Elisa Banchi, Alberto Pallavicini, Beatriz Novoa, Antonio Figueras
Summary: In this study, the spatial and temporal distribution of Perkinsus olseni, a protozoan parasite, was analyzed using sequencing and real-time PCR methods. The pathogen was found to be present in water and sediment samples, showing seasonal prevalence. It was also detected in clams and for the first time in Cerastoderma edule with low abundance and infection intensity levels.
Article
Fisheries
M. Monteiro, P. Perdiguero, A. Couto, C. R. Serra, P. Pereiro, B. Novoa, A. Figueras, L. Ribeiro, P. Pousao-Ferreira, C. Tafalla, A. Oliva-Teles, P. Enes, C. J. Secombes, P. Diaz-Rosales
Summary: Meagre, a potential candidate for Mediterranean aquaculture diversification, has understudied physiological consequences of immune system activation. This study evaluated the in vivo effects of lipo-polysaccharide on the spleen transcriptome of meagre using RNA-seq analysis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Marta Sendra, Martina Stampar, Katarina Fras, Beatriz Novoa, Antonio Figueras, Bojana Zegura
Summary: BPA is a widely used chemical compound in the manufacturing of human products, but it has been found to have endocrine disrupting and genotoxic effects. The industry has started developing BPA analogues, but there is insufficient data to determine their safety. Further research is needed to evaluate the risks of these analogues and ensure their safety for humans.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Fisheries
Patricia Pereiro, Antonio Figueras, Beatriz Novoa
Summary: In this study, the immune mechanisms involved in the resistance of Gilthead sea bream to RGNNV infection were elucidated through RNA-Seq analysis. The results showed that sea bream displayed a strong immune response characterized by the high expression of type I interferon-related genes, immune receptors, and antigen presentation-related genes in both brain and head kidney tissues. Additionally, complement-, coagulation-, and angiogenesis-related genes were highly enriched in the head kidney at the earlier sampling point. Interestingly, a neuroprotective scenario with restrained inflammation was observed in the brain of sea bream, unlike the activation of the stress axis and immunosuppression found in sea bass.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Raquel Rios-Castro, Adrian Cabo, Eva Teira, Claudio Cameselle, Susana Gouveia, Pedro Payo, Beatriz Novoa, Antonio Figueras
Summary: In this study, the DNA metabarcoding technique was used to analyze prokaryote diversity and community structure in wastewater and evaluate the efficiency of a wastewater treatment plant. The results showed significant differences in microbiomes among different sample types, with exclusive OTUs found in mussels, post-treatment discharge water, and raw wastewater. Seasonal variations were also observed in wastewater, potentially influenced by abiotic factors. The presence of potential pathogens in raw wastewater highlighted the importance of wastewater treatment, which was confirmed to effectively remove pathogens.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Immunology
Patricia Pereiro, Magali Rey-Campos, Antonio Figueras, Beatriz Novoa
Summary: In this study, the effects of sulfamethoxazole (SMX) and clarithromycin (CLA) on the transcriptome and metatranscriptome profiles of zebrafish were analyzed. The results showed that zebrafish exposed to antibiotics for two weeks had a higher bacterial load in the intestine and kidney, with significant differences in the relative abundance of certain bacterial classes observed only in the intestine. RNA-Seq analysis revealed that the complement/coagulation system was the most altered immune mechanism in the intestine, while the impact on the immune system in the kidney was not significant. Additionally, zebrafish treated with antibiotics showed a lower number of macrophages and neutrophils after 7 days of exposure.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Amaro Saco, Magali Rey-Campos, Beatriz Novoa, Antonio Figueras
Summary: In this study, mussels were challenged with viral haemorrhagic septicaemia virus (VHSV), and the viral load was eliminated after 24 hours. Mussels showed antiviral activity towards VHSV, indicating that the virus was recognized and eliminated by the mussel immune system, confirming that mussels are not VHSV vectors in the marine environment. The study also revealed the involvement of cytoplasmic viral sensors, JAK-STAT pathway activation, and various downstream antiviral effectors in the mussel immune response.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
J. G. Herranz-Jusdado, E. Morel, M. C. Ordas, D. Martin, F. Docando, L. Gonzalez, E. Sanjuan, P. Diaz-Rosales, M. Saura, B. Fouz, C. Tafalla
Summary: This study investigates the differentiation of B cells to plasmablasts/plasma cells in the skin and gills of rainbow trout in response to Yersinia ruckeri. The transcription levels of genes related to B cell function in both mucosal surfaces and in spleen and kidney were analyzed, and the presence and size of B cells, as well as the presence of plasmablasts secreting total or specific IgMs, were evaluated after infection. The results show that B cells in the skin and gills of rainbow trout can differentiate to plasmablasts/plasma cells in response to Y. ruckeri, and these cells can secrete specific IgMs as soon as 5 days after exposure.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
A. Panebianco, M. Rey-Campos, A. Romero, A. P. Diz, B. Novoa, A. Figueras
Summary: Haemocytes of Mytilus galloprovincialis are the main component of the internal self-defence system. The haemocytes in the intervalvar liquid share common characteristics with haemolymph haemocytes but also show significant differences in size, mobility, ROS production, and gene expression. These free intervalvar haemocytes could constitute the first line of defence as external sentinels.
FISH & SHELLFISH IMMUNOLOGY
(2023)