Article
Cell Biology
Taiki Satoh, Marcelo A. S. Toledo, Janik Boehnke, Kathrin Olschok, Niclas Flosdorf, Katrin Goetz, Caroline Kuestermann, Stephanie Sontag, Kristin Sere, Steffen Koschmieder, Tim H. Bruemmendorf, Nicolas Chatain, Yoh-ichi Tagawa, Martin Zenke
Summary: Dendritic cells (DC) are professional antigen-presenting cells that can be divided into different subsets, with the DC3 subset having proinflammatory properties. Human iPS cell-derived DC3 exhibit similar characteristics to blood DC3 in vitro. The JAK2 V617F mutation enhances DC3 production and biases towards erythrocytes and megakaryocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Somayeh Pouyanfard, Nairika Meshgin, Luisjesus S. Cruz, Karin Diggle, Hamidreza Hashemi, Timothy Pham, Manuel Fierro, Pablo Tamayo, Andrea Fanjul, Tatiana Kisseleva, Dan S. Kaufman
Summary: The study shows that human iPSC-derived macrophages can effectively reduce gene expression and pathological markers of liver fibrosis, offering a promising cell therapy approach to ameliorate fibrosis.
Article
Neurosciences
Christian Schinke, Valeria Fernandez Vallone, Andranik Ivanov, Yangfan Peng, Peter Koertvelyessy, Luca Nolte, Petra Huehnchen, Dieter Beule, Harald Stachelscheid, Wolfgang Boehmerle, Matthias Endres
Summary: Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect with no predictive biomarkers or preventive treatments available. Using sensory neurons derived from induced pluripotent stem cells as an experimental model for CIPN, researchers found differential responses to neurotoxic drugs between cell lines from different healthy donors, showcasing potential for studying CIPN pathogenesis and neuroprotective treatments.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Nelio A. J. Oliveira, Handan Sevim
Summary: Dendritic cells are important cells in the immune system, and researchers are looking for efficient methods to obtain dendritic cells from human induced pluripotent stem cells. Despite some challenges, progress has been made and there is potential for clinical applications.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Arisa Ito, Ke Ye, Masanari Onda, Nao Morimoto, Fumitaka Osakada
Summary: The study introduced an efficient and robust method for inducing RPE cells, despite the differentiation bias in hiPSC lines, which could help overcome line-to-line variations in RPE production.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Wei Cheng, Chengming Fan, Qing Song, Ping Chen, Hong Peng, Ling Lin, Cong Liu, Bin Wang, Zijing Zhou
Summary: Fibrotic diseases are responsible for a third of global deaths, leading to organ remodeling and dysfunction. Despite the complications of organ transplantation, induced pluripotent stem cell (iPSC)-based therapy shows potential as a treatment option by replacing fibrotic organs. Recent studies have successfully differentiated iPSCs into fibrosis-relevant cell types with anti-fibrotic effects, providing promising precision treatments for organ-specific fibrosis. This review summarizes the potential, advantages, challenges, and future outlook of iPSC-based approaches in treating organ fibrosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tsutomu Nakazawa, Ryosuke Maeoka, Takayuki Morimoto, Ryosuke Matsuda, Mitsutoshi Nakamura, Fumihiko Nishimura, Shuichi Yamada, Ichiro Nakagawa, Young-Soo Park, Hiroyuki Nakase, Takahiro Tsujimura
Summary: This study investigated the capability of human induced pluripotent stem cells (iPSCs) lysate-pulsed dendritic cells (DCs) to induce cancer-responsive cytotoxic T lymphocytes (CTLs) in vitro. The results demonstrated that the iPSCs/DCs induced sufficient CTL numbers that responded to specific cancer cells, suggesting the potential of iPSC/DCs as a promising universal cancer vaccine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Aeyung Kim, Seo-Young Lee, Sun-Ku Chung
Summary: The study found that caffeic acid (CAA) selectively eliminates undifferentiated induced pluripotent stem cells (iPSCs) and suppresses teratoma formation, suggesting its potential in preparing safe iPSC-based therapeutic cells.
Review
Biochemistry & Molecular Biology
Li-Jie Hsu, Chao-Lin Liu, Ming-Ling Kuo, Chia-Ning Shen, Chia-Rui Shen
Summary: Cell therapy involves using cells selected, manipulated, and treated outside the body to treat or prevent human disease. iPSCs are ideal cell sources due to their unique characteristics. NK cells are effective tumor killers without causing GVHD, making them excellent candidates for cell therapy.
Review
Cell Biology
Juan Huang, Qi Feng, Li Wang, Bingying Zhou
Summary: Cardiac diseases are the leading cause of global mortality, but limited progress has been made in developing therapeutic options. The use of animal models has limitations due to species-specific differences, leading to inaccurate drug testing results. Human pluripotent stem cell (hPSC) technology offers promising alternatives for heart disease modeling, cell therapy, and drug discovery, with potential for revolutionizing these fields with further optimization required.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Biotechnology & Applied Microbiology
Yanyan Chen, Pufeng Huang, Mengda Niu, Chuanhuizi Tian, Tingting Zhang, Zhiping Peng
Summary: This study developed an efficient culture method to generate functional CD8(+) T cells from human induced pluripotent stem cells (hiPSC) and obtained hiPS-CAR-T cells with high antigen-specific cytotoxicity that are not significantly different from CAR T cells found in peripheral blood. This finding may pave the way for the clinical use of hiPSC for cell-based cancer therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
