Article
Biophysics
Maria Elena Tarnok, Fanny Guzman, Luis F. Aguilar
Summary: This study investigated the influence of cholesterol content on membrane fusion facilitated by ISAV fusion peptides. Results showed that cholesterol affected membrane fusion, but the peptides did not require cholesterol in their membranes for fusion in the smallest lipid vesicles (0.2 μm LUV).
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Paola Lasso, Laura Rojas, Cindy Arevalo, Claudia Uruena, Natalia Murillo, Susana Fiorentino
Summary: In this study, it was found that the natural products Anamu-SC and P2Et have different mechanisms of antitumor activity. Anamu-SC reduces tumor growth in a murine mammary carcinoma model, while P2Et has no effect on melanoma. Both extracts decrease IL-10 levels in melanoma, but only P2Et increases TNF-α and IFN-γ levels. The extracts also have different effects on immune cell infiltration and the CD8+/Treg ratio.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jinjin Wang, Yan Li, Yawen Zhang, Longfei Chen, Lichun Fang, Shuang Chang, Yixin Wang, Peng Zhao
Summary: This study confirmed the pathogenicity of the chicken infectious anemia virus (CIAV) detected in contaminated vaccines through reverse genetic assays. The virus caused anemia and immune suppression in poultry. This highlights the significance of vaccine contamination and the potential risks associated with it.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Wenlong Cai, Surendra Kumar, Umasuthan Navaneethaiyer, Albert Caballero-Solares, Laura A. Carvalho, Shona K. Whyte, Sara L. Purcell, Nellie Gagne, Tiago S. Hori, Melissa Allen, Richard G. Taylor, Rachel Balder, Christopher C. Parrish, Matthew L. Rise, Mark D. Fast
Summary: This study investigated the impact of four experimental functional feeds on Atlantic salmon during a single infection with sea lice and a co-infection with sea lice and infectious salmon anemia virus (ISAv). Transcriptomic analysis revealed different gene expression responses in the skin tissues of fish with different experimental diets. The co-infection group showed lower survival rates and up-regulated genes associated with glycolysis and immune response pathways. Understanding the effects of experimental functional feeds on the host response and co-infection trajectory is crucial for developing effective intervention strategies in open cage culture.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Johanna Hol Fosse, Adriana Magalhaes Santos Andresen, Frieda Betty Ploss, Simon Chioma Weli, Inger Austrheim Heffernan, Subash Sapkota, Krister Lundgard, Raoul Valentin Kuiper, Anita Solhaug, Knut Falk
Summary: Many sialic acid-binding viruses express a receptor-destroying enzyme (RDE) that removes the virus-targeted receptor and limits viral interactions with host cells. In this study, it was found that infectious salmon anemia virus (ISAV) progressively destroys the ISAV receptor on erythrocytes, leading to a loss of binding capacity for new virus particles. This surface modulation is mediated by the haemagglutinin esterase (HE) and is not influenced by endogenous esterases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Yuhan Yuan, Yuanyuan Zhang, Jing Chen, Chunxia Huang, Haimei Liu, Wenlong Li, Lijuan Liang, Yi Wang, Yunjun Liu
Summary: A new ligand DTOIP was synthesized and combined with three iridium(III) complexes to form Ir1, Ir2, and Ir3. The complexes showed high antiproliferative activity against B16 cells by inducing apoptosis through mitochondrial entry, decreasing glutathione levels, increasing intracellular ROS content, and causing DNA damage. In vivo experiments demonstrated that Ir1 effectively inhibits tumor growth.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Hai-Jun Hu, Xiu Liang, Hai-Lang Li, Huai-Yuan Wang, Jin-Fa Gu, Lan-Ying Sun, Jing Xiao, Jin-Qing Hu, Ai-Min Ni, Xin-Yuan Liu
Summary: The combination therapy of localized ZD55-IL-24 and systemic PD-1 blockade shows a synergistic inhibition of both local and distant established tumors in an immunocompetent mouse model. This synergistic therapeutic effect is associated with the promotion of tumor immune infiltration and recognition, primarily facilitated by ZD55-IL-24 therapy. The study suggests a rationale for further investigation of this combination therapy in clinical settings.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Fisheries
Margo E. Chase-Topping, Chris Pooley, Hooman K. Moghadam, Borghild Hillestad, Marie Lillehammer, Lene Sveen, Andrea Doeschl-Wilson
Summary: The experiment tested the impact of vaccination and selective breeding on the transmission of Infectious salmon anemia virus in Atlantic salmon, finding that both methods reduced the probability of infection in contact fish. Genetic resistance showed a more significant effect on infection endurance compared to vaccination.
Article
Biochemistry & Molecular Biology
Ali Zari, Hajer Alfarteesh, Carly Buckner, Robert Lafrenie
Summary: Uncaria tomentosa demonstrated potent anti-cancer activity both in vitro and in vivo, particularly with its ethanolic extracts; it reduced tumor size and weight, decreased the proliferation marker Ki-67, inhibited angiogenic markers, and reduced T-cell infiltration into tumors.
Article
Fisheries
Liming Xu, Jingzhuang Zhao, Miao Liu, Guangming Ren, Shi Cheng Han, Yizhi Shao, Yongsheng Cao, Hongbai Liu, Tongyan Lu
Summary: IHNV enhances the multiplication of IPNV during co-infection, with the enhancement becoming stronger with increasing infection time interval, regardless of the infection order.
Article
Food Science & Technology
Yuanhui Li, Peng Wang, Zejun Zhang, Qinghong Liu
Summary: A novel lectin named PML was purified from the fruiting bodies of the edible mushroom Phellodon melaleucus. The purified PML showed high hemagglutination activity and was stable under certain pH and temperature conditions. PML treatment inhibited tumor growth and increased IL-10 content in a B16 melanoma mouse model, suggesting its potential as an anticancer therapeutic agent.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Veterinary Sciences
Chrissy D. Eckstrand, Brandi K. Torrevillas, Rebecca M. Wolking, Daniel S. Bradway, Janet V. Warg, Richard D. Clayton, Laura B. Williams, Allan P. Pessier, Joetta Lynn Reno, Kathleen M. McMenamin-Snekvik, Jim Thompson, Timothy Baszler, Kevin R. Snekvik
Summary: Rapid growth in aquaculture has led to the emergence of diseases in high-density production systems. Detection and surveillance methods for infectious diseases are crucial for rapid identification and response. A proof-of-concept approach for virus detection using infectious salmon anemia virus (ISAV) as the archetype pathogen was implemented, integrating histopathology, virus isolation, whole-genome sequencing, electron microscopy, in situ hybridization, and reverse transcription real-time PCR.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Oncology
Sisi Liu, Mingxin Li, Fengqiao Sun, Junwen Zhang, Fusheng Liu
Summary: The combination treatment of oncolytic herpes simplex type I virus (oHSV-1) and TLR3 agonist poly(I:C) has been found to significantly improve the therapeutic effect on uveal melanoma (UM), generating immune responses and enhancing anti-tumoral activity.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Cao Dai Phung, Tuan Hiep Tran, Hanh Thuy Nguyen, Tien Tiep Nguyen, Jee-Heon Jeong, Sae Kwang Ku, Chul Soon Yong, Han-Gon Choi, Jong Oh Kim
Summary: Efforts in developing cancer vaccines have faced challenges, but a new artificial nanovaccine targeting lymphatic dendritic cells shows promise in boosting immune response and controlling immunosuppression simultaneously. By incorporating IL-10 siRNA to inhibit suppressive cytokine secretion and utilizing a CD205 antibody for enhanced dendritic cell targeting, the nanovaccine demonstrates potential for enhancing antitumor immunity.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Agriculture, Dairy & Animal Science
Rachel B. Breyta, William N. Batts, Gael Kurath
Summary: Understanding the transmission of viruses between host populations is crucial for developing strategies to control viral diseases. This study identified three transmission links associated with the emergence of IHNV in coastal Washington steelhead trout populations between 2007 and 2011, providing insight into the virus movement from source to recipient populations. The findings suggest at least three introductions of virus from Columbia fish to coastal fish, with infected juvenile fish in the Columbia region being the likely source and adult fish returning to coastal hatcheries as the first detected recipient populations. Variations in timing and distance of these transmission links highlight the complexity of IHNV transmission routes.
Article
Virology
Carlos Barrera-Avalos, Roberto Luraschi, Eva Vallejos-Vidal, Maximiliano Figueroa, Esteban Arenillas, Daniela Barria, Felipe Hernandez, Carlos Mateluna, Javier Mena, Claudia Rioseco, Claudia Torrent, Claudio Vergara, Gaby Gutierrez, Javiera Quiroz, Javiera Alarcon, Julio Cartagena, Javiera Cayunao, Andrea Mella-Torres, Alvaro Santibanez, Sebastian Tapia, Alejandro Undurraga, Deborah Vargas, Valentina Wong, Ailen Inostroza-Molina, Daniel Valdes, Monica Imarai, Claudio Acuna-Castillo, Felipe E. Reyes-Lopez, Ana M. Sandino
Summary: This study compared the performance of different viral transport media in COVID-19 diagnosis in Santiago, Chile, finding DNA/RNA Shield (TM) to be more effective. PBS medium may compromise sample diagnosis, while NAT, Ezmedlab, VTM-N, and DNA/RNA Shield (TM) media show acceptable RT-qPCR parameters.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Alejandro Escobar, Felipe E. Reyes-Lopez, Monica L. Acevedo, Luis Alonso-Palomares, Fernando Valiente-Echeverria, Ricardo Soto-Rifo, Hugo Portillo, Jimena Gatica, Ivan Flores, Estefania Nova-Lamperti, Carlos Barrera-Avalos, Maria Rosa Bono, Leonardo Vargas, Valeska Simon, Elias Leiva-Salcedo, Cecilia Vial, Juan Hormazabal, Lina Jimena Cortes, Daniel Valdes, Ana M. Sandino, Monica Imarai, Claudio Acuna-Castillo
Summary: CoronaVac vaccine induces robust humoral immune response and at least 90 days of cellular immune memory in vaccinated volunteers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Carlos Barrera-Avalos, Roberto Luraschi, Eva Vallejos-Vidal, Andrea Mella-Torres, Felipe Hernandez, Maximiliano Figueroa, Claudia Rioseco, Daniel Valdes, Monica Imarai, Claudio Acuna-Castillo, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: This study evaluated the sensitivity and specificity of RAT (SD BIOSENSOR, South Korea) widely used for COVID-19 testing and diagnosis in Santiago of Chile, and found that it had a certain effectiveness in detecting the virus. However, it is important to be cautious of its potential impact from new viral variants.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Tejon, Nicolas Valdivieso, Felipe Flores-Santibanez, Veronica Barra-Valdebenito, Victor Martinez, Mario Rosemblatt, Daniela Sauma, Maria Rosa Bono
Summary: This study investigated the phenotype and function of peritoneal macrophages in a murine model of SLE. The results showed that macrophages from diseased mice exhibited altered phenotype and function, shifting towards a pro-inflammatory state. Additionally, there was an accumulation of B1a cells in the peritoneal cavity. These findings contribute to a better understanding of SLE and may aid in the development of novel therapies.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Virology
Matias Cardenas, Sofia Michelson, Daniel R. Perez, Margarita Montoya, Jorge Toledo, Yesseny Vasquez-Martinez, Marcelo Cortez-San Martin
Summary: This study reveals the different roles of each gene segment in viral replication, infectivity, and cellular immune response in infectious salmon anemia virus (ISAV). Segments 5 and 6 play a critical role in the virulence and infectivity of the virus, while segment 2 can modulate the cellular immune response. The genetic compatibility between the genomic segments of HPR7b and HPR0 poses a latent risk of reassortment.
Article
Public, Environmental & Occupational Health
Carlos Barrera-Avalos, Roberto Luraschi, Claudio Acuna-Castillo, Mabel Vidal, Andrea Mella-Torres, Ailen Inostroza-Molina, Rodrigo Vera, Sergio Vargas, Ivan Hernandez, Christian Perez, Eva Vallejos-Vidal, Daniel Valdes, Monica Imarai, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: Vaccine administration is an efficient way to control the COVID-19 pandemic, but the emergence of SARS-CoV-2 variants can evade vaccine-induced immunity. This report provides evidence of clinical symptom differences between vaccinated and unvaccinated patients infected with the VOI B.1.621 (Mu) variant, suggesting the protective effects of CoronaVac against this variant.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Public, Environmental & Occupational Health
Claudio Acuna-Castillo, Mabel Vidal, Ailen Inostroza-Molina, Eva Vallejos-Vidal, Roberto Luraschi, Maximiliano Figueroa, Carlos Barrera-Avalos, Rodrigo Vera, Sergio Vargas, Daniel Valdes, Kevin Maisey, Monica Imarai, Elias Leiva-Salcedo, Alejandro Escobar, Sebastian Reyes-Cerpa, Alexis Gaete, Ricardo Palma-Vejares, Dante Travisany, Leonel E. Rojo, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: The identification and tracking of SARS-CoV-2 infected patients are essential for limiting the spread of COVID-19. This study identified a case of reinfection in a homeless person, highlighting the importance of genomic surveillance programs.
JOURNAL OF ENVIRONMENTAL AND PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Roberto Luraschi, Carlos Barrera-Avalos, Eva Vallejos-Vidal, Javiera Alarcon, Andrea Mella-Torres, Felipe Hernandez, Ailen Inostroza-Molina, Daniel Valdes, Monica Imarai, Claudio Acuna-Castillo, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: This study analyzes the performance of two commercial RT-qPCR kits used in Chile and finds that the TAAG kit has lower sensitivity, potentially leading to false negatives and local outbreaks of infection.
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Claudio Acuna-Castillo, Ailen Inostroza-Molina, Sergio A. Castro, Sonia Molina-Cabrera, Elias Leiva-Salcedo, Denise Riquelme, Roberto Luraschi, Carlos Barrera-Avalos, Eva Vallejos-Vidal, Andrea Mella-Torres, Daniel Valdes, Claudio Torres, Kevin Maisey, Alejandro Escobar, Sebastian Reyes-Cerpa, Daniela Toro-Ascuy, Monica Imarai, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: This study provides a retrospective and comparative analysis of the behavior during the first and second waves of the COVID-19 pandemic in Chile. The findings suggest a decrease in mortality rate and a shift towards infections in young patients with reduced comorbidities in the second wave. Vaccinated individuals experience shorter duration of symptoms, while patients with more comorbidities are more likely to develop severe illness. This report contributes to our partial understanding of the behavior and critical factors in the severity of the COVID-19 pandemic in the population of Santiago, Chile.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Daniela Carrillo, Natalie Edwards, David Arancibia-Altamirano, Fabiola Otarola, Cynthia Villarroel, Catalina P. Prieto, Maria Gabriela Villamizar-Sarmiento, Daniela Sauma, Fernando Valenzuela, Jose Lattus, Felipe Oyarzun-Ampuero, Veronica Palma
Summary: This study evaluated the effect of Wharton's gelatin MSC secretome (hWJCM) in a psoriasiform-like dermatitis (PD) mouse model and found that hWJCM significantly improved the PD phenotype and reduced aberrant angiogenesis and discomfort. The topical application of HA hWJCM may be an effective noninvasive therapeutic solution for psoriasis and other skin diseases.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Public, Environmental & Occupational Health
Claudio Acuna-Castillo, Carlos Barrera-Avalos, Vivienne C. Bachelet, Luis A. Milla, Ailen Inostroza-Molina, Mabel Vidal, Roberto Luraschi, Eva Vallejos-Vidal, Andrea Mella-Torres, Daniel Valdes, Felipe E. E. Reyes-Lopez, Monica Imarai, Patricio Rojas, Ana Maria Sandino
Summary: This study reveals that the Omicron variant is responsible for the highest number of reinfection cases in Chile, highlighting its potential for immune evasion. This important epidemiological information contributes to the development and implementation of effective public health policies.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Infectious Diseases
Claudio Acuna-Castillo, Kevin Maisey, Mabel Vidal, Carlos Barrera-Avalos, Ailen Inostroza-Molina, Roberto Luraschi, Eva Vallejos-Vidal, Daniel Valdes, Monica Imarai, Felipe E. Reyes-Lopez, Ana Maria Sandino
Summary: The study reports a case of SARS-CoV-2 persistence for 386 days in a health worker from Santiago de Chile, which could be one of the longest reported viral persistence events. RNA sequencing analyses showed that the first and second positive results belonged to Clade 20A variant, but the second positive result had no presence or circulation in Chile since May 2021. The study highlights the importance of testing for viral absence, identifying potential persistence, and minimizing the risk of local outbreaks after completing quarantine.
INFECTIOUS DISEASE REPORTS
(2022)