Article
Genetics & Heredity
Sung Hye Kim, David A. MacIntyre, Lynne Sykes, Maria Arianoglou, Phillip R. Bennett, Vasso Terzidou
Summary: This study examined the effect of whole blood holding time on the stability of plasma miRNA expression profiles. The results demonstrated that holding time can have dramatic effects on analytical reliability and reproducibility.
FRONTIERS IN GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Lin Li, Andre Gorgens, Veronika Mussack, Elena Pepeldjiyska, Anne Sophie Hartz, Andreas Rank, Jorg Schmohl, Doris Kra''mer, Samir El Andaloussi, Michael W. Pfaffl, Helga Schmetzer
Summary: Extracellular Vesicles (EVs) play a crucial role in hematological malignancies, affecting bone marrow environment, immune system, drug resistance, and therapies. EVs collected from serum of leukemia samples have potential as biomarkers for disease pattern distinctions. This study aims to investigate the potential role and methodology of serum-derived EVs as biomarkers in leukemic versus healthy samples.
CANCER GENE THERAPY
(2022)
Article
Immunology
Sonya Krishnan, Artur T. L. Queiroz, Amita Gupta, Nikhil Gupte, Gregory P. Bisson, Johnstone Kumwenda, Kogieleum Naidoo, Lerato Mohapi, Vidya Mave, Rosie Mngqibisa, Javier R. Lama, Mina C. Hosseinipour, Bruno B. Andrade, Petros C. Karakousis
Summary: Novel high-throughput approaches may improve the recognition of tuberculosis in HIV-infected individuals, especially in those with severely suppressed immune systems.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Patrick Altmann, Markus Ponleitner, Paulus Stefan Rommer, Helmuth Haslacher, Patrick Mucher, Fritz Leutmezer, Axel Petzold, Christoph Wotawa, Rupert Lanzenberger, Thomas Berger, Henrik Zetterberg, Gabriel Bsteh
Summary: The study found that the soluble serum and plasma NfL concentration remains stable when unprocessed blood samples are stored up to 7 days at room temperature, which is crucial for ensuring reliable study protocols.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Kuno Suzuki, Tatsuya Yamaguchi, Masakazu Kohda, Masami Tanaka, Hiroyuki Takemura, Mitsuru Wakita, Yoko Tabe, Shunsuke Kato, Motomi Nasu, Takashi Hashimoto, Shinji Mine, Nobuko Serizawa, Ko Tomishima, Akihito Nagahara, Takahisa Matsuda, Taiki Yamaji, Shoichiro Tsugane, Yutaka Saito, Hiroyuki Daiko, Takaki Yoshikawa, Ken Kato, Takuji Okusaka, Takahiro Ochiya, Yusuke Yamamoto, Shoji Yotsui, Takashi Yamamoto, Tomoyuki Yamasaki, Hiroshi Miyata, Masayoshi Yasui, Takeshi Omori, Kazuyoshi Ohkawa, Kenji Ikezawa, Tasuku Nakabori, Naotoshi Sugimoto, Toshihiro Kudo, Keiichi Yoshida, Masayuki Ohue, Takashi Nishizawa
Summary: This study investigated the expression of microRNAs (miRNAs) in blood and its relationship with various diseases. The results showed that the use of ethylenediaminetetraacetic acid (EDTA) as an anticoagulant and specific storage conditions can contribute to highly reproducible miRNA expression profiles. Additionally, standardization of these preanalytical conditions can reduce the variation in sample quality across different facilities.
Review
Oncology
Jose D. Debes, Pablo A. Romagnoli, Jhon Prieto, Marco Arrese, Angelo Z. Mattos, Andre Boonstra
Summary: Hepatocellular carcinoma (HCC) is a major cause of cancer death worldwide. Late diagnosis and limited treatment options contribute to the low survival rate of patients with HCC. Currently, combining ultrasound with the biomarker AFP is a commonly used screening method, but its clinical utility and operator-dependence are controversial.
Article
Biochemistry & Molecular Biology
Christiane Muehle, Johannes Kornhuber
Summary: The activity of neutral sphingomyelinase (NSM) has been detected in human serum and plasma samples, which hydrolyzes fluorescently labeled sphingomyelin to ceramide. This discovery could lead to the development of new biomarkers or therapeutic targets for diseases affected by sphingolipid derangements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Hongdong Li, Liyuan Ma, Fengyuan Luo, Wenkai Liu, Na Li, Tao Hu, Haijian Zhong, You Guo, Guini Hong
Summary: This study utilized a large miRNA-profiled serum samples repository to identify two miRNA pairs that can accurately diagnose glioma and differentiate it from other brain diseases. These findings provide potential population screening and cancer-specific biomarkers for glioma clinical practice.
Article
Endocrinology & Metabolism
Elena Flowers, Bradley E. Aouizerat, Alka M. Kanaya, Jose C. Florez, Xingyue Gong, Li Zhang
Summary: This study identified 5 miRs (miR-144, miR-186, miR-203a, miR-205, miR-206) that can effectively predict the incidence of type 2 diabetes. After adjusting for covariates, the HR for the highest quartile risk group compared with the lowest quartile risk group was 5.91. There was significant interaction between the intensive lifestyle and metformin groups compared with placebo. These findings may have potential clinical utility for risk stratification of individuals with prediabetes.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Immunology
Jun-Nan Lv, Jia-Qi Li, Ying-Bin Cui, Yuan-Yuan Ren, Ya-Jing Fu, Yong-Jun Jiang, Hong Shang, Zi-Ning Zhang
Summary: Approximately 10-40% of HIV-infected individuals fail to achieve immune function improvement after ART, increasing the risk of non-AIDS-related diseases. This study identified five upregulated miRNAs in the plasma of immune non-responders, which were negatively correlated with CD4(+) T cell counts. A predictive model using these miRNAs accurately distinguished between immune non-responders and responders, with further mechanistic insights provided on the role of miR-16-5p in CD4(+) T cell function regulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jaehoon Lee, Hee Seung Lee, Soo Been Park, Chanyang Kim, Kahee Kim, Dawoon E. Jung, Si Young Song
Summary: The study aimed to identify non-invasive miRNA markers for early pancreatic cancer diagnosis. A total of 39 miRNA markers were selected from blood serum samples to build a PC diagnosis model, which showed excellent performance in independent sample validation and differential expression in samples from PC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Melanie D. Smith, Shalem Y. Leemaqz, Tanja Jankovic-Karasoulos, Dale McAninch, Dylan McCullough, James Breen, Claire T. Roberts, Katherine A. Pillman
Summary: This study proposes a method for detecting haemolysis in miRNA high-throughput sequencing data and establishes a haemolysis signature. The results demonstrate that the abundance of miRNA in blood plasma is affected by haemolysis. The feasibility of this signature is further validated through validation experiments.
Article
Oncology
Elena Flowers, Isabel Elaine Allen, Alka M. Kanaya, Bradley E. Aouizerat
Summary: Circulating microRNAs were found to be significant predictors of fasting blood glucose levels in individuals with prediabetes, with some microRNAs associated with risk for type 2 diabetes. This longitudinal study assessed whether microRNAs could predict changes in fasting blood glucose levels over time, providing new insights into the complex etiology of type 2 diabetes and responses to behavioral interventions.
BIOMARKER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Xinyue Zhang, Ruling Yang, Mengjie Ma, Siyu Chen, Kaiyin Guo, Li Zhang, Yingnan You, Hailin Jia, Bo Han
Summary: This study investigated the plasma exosome miRNA profile in pediatric fulminant myocarditis (FM) using small RNA microarray. Differential expression of 266 miRNAs was observed, including 197 upregulated and 69 downregulated candidate genes. Through analysis, FM-specific target genes related to inflammation and/or the immune system were identified. CeRNA networks between cardiomyocytes and peripheral blood mononuclear cells were established, and specific miRNAs were validated as potential biomarkers for FM diagnosis.
Article
Biochemistry & Molecular Biology
Maia Segura-Wang, Bertrand Grenier, Suzana Ilic, Ursula Ruczizka, Maximiliane Dippel, Moritz Buenger, Matthias Hackl, Veronika Nagl
Summary: This study investigated the changes in miRNA expression induced by DON in porcine liver, jejunum, and serum. The serum miRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual miRNAs were significant, and a combination of four miRNAs increased the predictive capacity for DON exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
