Article
Dentistry, Oral Surgery & Medicine
Jishnu Krishna Kumar, Padmapriya Surendranath, Rajalakshmanan Eswaramoorthy
Summary: Conventional endodontic treatment of young permanent teeth with necrotic pulp has a poor prognosis. Regenerative endodontics, such as revascularization using platelet-rich fibrin, provides a successful alternative treatment for immature nonvital permanent teeth.
Article
Health Care Sciences & Services
Sawsan T. Abu Zeid, Ruaa A. Alamoudi, Osama S. Alothmani, Abeer A. Mokeem Saleh, Amna Y. Siddiqui
Summary: This study evaluated the long-term outcomes of regenerative endodontics in immature necrotic permanent teeth and found successful clinical and radiographic results with appropriate case selection.
Review
Dentistry, Oral Surgery & Medicine
Qianwei Tang, Hua Jin, Song Lin, Long Ma, Tingyu Tian, Xiurong Qin
Summary: Platelet concentrates can be used as successful scaffolds for regenerative endodontic treatment of necrotic immature permanent teeth, and PRP as a scaffold may achieve better periapical healing of teeth with periapical inflammation, although they did not differ significantly from conventional blood clot scaffolds in development of the root.
Article
Dentistry, Oral Surgery & Medicine
Zafer C. Cehreli, Gizem Erbas Unverdi, Elif Ballikaya
Summary: This case series demonstrates the clinical and radiographic outcomes of regenerative endodontic treatment (RET) using deciduous pulp autotransplantation in traumatized, necrotic young permanent incisors. Follow-up observations at 12 and 24 months showed radiographic evidence of complete periapical healing, slight increase in dentinal wall thickness, and continued apical closure in all patients.
JOURNAL OF ENDODONTICS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Ali Nosrat, Behnam Bolhari, Shima Saber Tahan, Omid Dianat, Paul Michael Howell Dummer
Summary: Revitalizing root canals of previously treated teeth with open apices using regenerative endodontic treatment (RET) shows promising results, but further high-quality clinical studies are needed to confirm its viability.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Review
Health Care Sciences & Services
Paulina Pietruszka, Izabela Chruscicka, Irena Dus-Ilnicka, Anna Paradowska-Stolarz
Summary: This article discusses the use of platelet-rich materials in dentistry treatments and compares different methods of obtaining them and their potential uses. These materials have great potential in regenerative personalized medicine.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Multidisciplinary Sciences
Shangting You, Yi Xiang, Henry H. Hwang, David B. Berry, Wisarut Kiratitanaporn, Jiaao Guan, Emmie Yao, Min Tang, Zheng Zhong, Xinyue Ma, Daniel Wangpraseurt, Yazhi Sun, Ting-yu Lu, Shaochen Chen
Summary: Three-dimensional bioprinting techniques are widely used for fabricating 3D-engineered tissues, but face challenges in maintaining high cell density, cell viability, and fabrication resolution simultaneously. A novel approach using iodixanol in bioink was developed to reduce light scattering and improve fabrication resolution. Tissues with high cell density and fine vascular networks were successfully fabricated, and showed viability and endothelialization after 14 days of culture in a perfusion system.
Article
Dentistry, Oral Surgery & Medicine
Juliana S. Ribeiro, Carolina K. Sanz, Eliseu A. Munchow, Nikhil Kalra, Nileshkumar Dubey, Carlos Enrique C. Suarez, J. Christopher Fenno, Rafael G. Lund, Marco C. Bottino
Summary: This study aimed to develop photocrosslinkable CHX/GelMA hydrogels with broad spectrum anti-bacterial action as a clinically viable cell-friendly disinfection therapy for regenerative endodontics.
Article
Engineering, Biomedical
Qian Li, Zhiai Hu, Yongxi Liang, Cancan Xu, Yi Hong, Xiaohua Liu
Summary: The study developed a multifunctional peptide-conjugated, pH-sensitive non-viral gene vector for fast revascularization and pulp regeneration in a full-length human tooth root. This vector significantly increased gene transfection efficiency, reduced cytotoxicity, upregulated VEGF expression in dental pulp stem cells, and accelerated new blood vessel formation and pulp tissue regeneration. The approach proves to be safe and effective for regenerative endodontics.
ACTA BIOMATERIALIA
(2021)
Article
Pharmacology & Pharmacy
Weijie Zou, Shuang Yang, Li Chen, Su Hu, Guangyu Hao, Chunhong Hu
Summary: Iodinated contrast media, particularly iodixanol, is commonly used in radiological examinations and interventional therapy. However, iodixanol has a higher incidence of delayed cutaneous adverse reactions compared to other contrast media. The mechanisms behind these reactions involve increased degranulation of mast cells and synthesis of inflammatory factors, which are mediated by PLC-gamma and PI3K-related pathways. Trigonelline has been shown to inhibit iodixanol-induced pseudo-allergic reactions in both in vitro and in vivo settings.
Article
Engineering, Biomedical
He Liu, Jing Lu, Qianzhou Jiang, Markus Haapasalo, Junrong Qian, Franklin R. Tay, Ya Shen
Summary: Regenerative endodontic procedures have been widely used in clinical endodontics as a valuable adjunct to conventional strategies. Biomaterial scaffolds have shown clinical potential and remarkable advancements in regenerative endodontics.
BIOACTIVE MATERIALS
(2022)
Article
Biophysics
Colleen A. Roosa, Ismaeel Muhamed, Ashlyn T. Young, Kimberly Nellenbach, Michael A. Daniele, Frances S. Ligler, Ashley C. Brown
Summary: A new high throughput method for generating fibrin nanoparticles using probe sonication was reported in this study, which can promote clot formation and cell migration. When combined with fibrinogen, sonicated fibrin nanoparticles can form a fibrin gel and support cell migration, which is relevant for wound healing. The results suggest that sonicated fibrin nanoparticles may be a promising therapy for wound healing that is easy to produce and deliver in a flowable formulation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yasmine Elhamouly, Rania M. El Backly, Dalia M. Talaat, Samia S. Omar, Maha El Tantawi, Karin M. L. Dowidar
Summary: This study compared the outcomes of Biodentine and 70S30C-BAG as pulpotomy agents in primary teeth, showing that Biodentine demonstrated better clinical, histologic, and anti-inflammatory outcomes compared to 70S30C-BAG, promoting pulp healing and regeneration. 70S30C-BAG resulted in pulp necrosis due to persistent inflammation, leading to clinical failure.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Health Care Sciences & Services
Raphael Richert, Julie Santamaria, Laurent Laforest, Jean-Christophe Maurin
Summary: This study assessed the medical, clinical, and radiographic aspects of multiple idiopathic resorptions (MIR) and examined the factors associated with the risk of extraction. The findings suggest that MIR often require extraction and pose challenges for prosthetic rehabilitation.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Engineering, Multidisciplinary
Naresh Kumar, Nazrah Maher, Faiza Amin, Hani Ghabbani, Muhammad Sohail Zafar, Francisco Javier Rodriguez-Lozano, Ricardo E. Onate-Sanchez
Summary: This paper thoroughly reviews and discusses various pulp-regenerative approaches and materials used in regenerative endodontics, highlighting current trends and future research prospects in this field.
Review
Dentistry, Oral Surgery & Medicine
Matthias Zehnder, Georgios N. Belibasakis
Summary: This narrative review discusses the use of biomarkers in clinical Endodontic research, highlighting the importance of addressing clinical problems through suitable diagnostic biomarkers. The study focuses on two clinical issues, reversible versus irreversible pulpitis and persistent idiopathic dentoalveolar pain, to demonstrate the role of biomarkers in understanding complex dental pathologies and guiding appropriate treatment strategies.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Frank Sanner, David Sonntag, Norbert Hambrock, Matthias Zehnder
Summary: Persistent idiopathic dentoalveolar pain (PIDAP) mainly affects females and is typically associated with undisturbed sleep and periodontal allodynia.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
N. Ballal, H. F. Duncan, D. B. Wiedemeier, N. Rai, P. Jalan, V Bhat, V. S. Belle, M. Zehnder
Summary: In this study, it was found that the levels of neutrophil gelatinase 9 (MMP-9)/total protein (TP) and NaOCl lavage significantly influenced pulp survival in cariously exposed and directly capped teeth, while mild preoperative discomfort did not have an impact.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Chemistry, Physical
Chafik Ghayor, Indranil Bhattacharya, Julien Guerrero, Mutlu oezcan, Franz E. Weber
Summary: This study investigates the role of microarchitecture and nanoarchitecture of scaffolds in osteoconduction. The results show that microporosity of the scaffolds does not affect bony ingrowth or osteoclastic resorbability. This research is important for preventing non-unions during bone regeneration procedures.
Article
Cell Biology
Chai Foong Lai, Juliet Shen, Anamaria Balic, Pierfrancesco Pagella, Martin E. Schwab, Thimios A. Mitsiadis
Summary: The study identified the widespread distribution of Nogo-A in human teeth, and clarified its significant regulatory role in the development of dental pulp stem cells, indicating its potential application in clinics.
Article
Multidisciplinary Sciences
Thimios A. Mitsiadis, Lucia -Rojo, Anamaria Balic, Silvio Weber, Paul Saftig, Pierfrancesco Pagella
Summary: The Adam10/Notch axis controls dental epithelial cell boundaries, cell fate switch, and proper enamel formation in continuously growing incisors.
Article
Dentistry, Oral Surgery & Medicine
Anas Al-Jadaa, Zehraa Saidi, Maise Mahmoud, Rahaf Al-Taweel, Matthias Zehnder
Summary: This study compared the in vitro efficacy and safety of different irrigant activation devices in apically closed and open root canal systems. The results showed that ultrasonic agitation was more effective in cleaning the central area of the root canal, while it had a lower effect in cleaning the simulated periapical lesion. Therefore, ultrasonic agitation was found to be more targeted and safer after the administration of sodium hypochlorite.
JOURNAL OF ENDODONTICS
(2023)
Article
Biotechnology & Applied Microbiology
Christophe Drouet, Nicolas Vandecandelaere, Anke Burger-Kentischer, Iris Trick, Christina G. Kohl, Tanja Maucher, Michaela Mueller, Franz E. Weber
Summary: Biomimetic nanocrystalline apatites analogous to bone mineral can be prepared using soft chemistry. However, immersion in aqueous media of dried non-carbonated biomimetic apatite crystals may generate an acidification event, which could limit their further development. In this study, the acidification phenomenon was explored, and a practical strategy to circumvent it was proposed. The non-cytotoxicity and antibacterial features of the post-equilibrated nanocrystalline apatites were demonstrated, suggesting their relevance in the field of antibacterial bone substitutes.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Thimios A. Mitsiadis, Pierfrancesco Pagella, Terence D. Capellini, Moya Meredith Smith
Summary: The Notch pathway is involved in cell fate specification and embryonic development. The Jagged2 gene, which encodes a ligand for Notch receptors, is expressed early in tooth development. Mutations in Jagged2 result in abnormal tooth morphology and impaired enamel deposition. The Notch signaling cascade is disrupted in Jagged2 mutant teeth, leading to dental structures more reminiscent of fish enamel. We propose that the increased number of Notch homologues in metazoa enabled the formation and maintenance of distinctive cell fates within organs and tissues during evolution.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julia Rieber, Gabriella Meier-Burgisser, Iris Miescher, Franz E. Weber, Petra Wolint, Yang Yao, Esteban Ongini, Athanasios Milionis, Jess G. Snedeker, Maurizio Calcagni, Johanna Buschmann
Summary: Tendon injuries can result in limited range of motion and poor biomechanical outcomes. A novel three-layer tube based on polymer DegraPol with incorporated IGF-1 was developed using emulsion electrospinning. The IGF-1-containing tubes exhibited sustained release of the growth factor and showed bioactivity by upregulating gene expression. They also demonstrated superior mechanical properties compared to pure DP tubes. These tubes may help accelerate the healing process and reduce adhesion formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julien Guerrero, Ekaterina Maevskaia, Chafik Ghayor, Indranil Bhattacharya, Franz E. Weber
Summary: This study utilized additive manufacturing to produce two different diameter tricalcium phosphate-based bone scaffolds and observed them in vivo for 10 days followed by gene sequencing and histological analysis. The results showed upregulation of genes related to immune response, cell adhesion, and cell migration in both scaffolds. However, only the scaffold with a 0.50mm diameter showed significant overexpression of genes involved in angiogenesis, cell differentiation regulation, ossification, and bone development. Immunohistochemistry and high-resolution CT scans also revealed more blood vessels and mineralized tissue in the 0.50mm scaffold. Therefore, different filament diameters significantly influence angiogenesis and cell differentiation regulation in the early phase of bone regeneration, impacting the overall clinical outcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Thimios A. Mitsiadis, Pierfrancesco Pagella, Helder Gomes Rodrigues, Alexander Tsouknidas, Liza L. Ramenzoni, Freddy Radtke, Albert Mehl, Laurent Viriot
Summary: Evolutionary changes in vertebrates are often accompanied by genetic alterations that affect tooth crown shape. The Notch pathway, a highly conserved signaling pathway, plays a crucial role in morphogenetic processes, including tooth development. Experimental studies on developing mouse molars showed that the loss of the Notch-ligand Jagged1 leads to minor modifications in tooth crown shape, similar to those observed during the evolution of Muridae species. RNA sequencing analysis revealed that these alterations are caused by changes in the expression of over 2000 genes, and that Notch signaling interacts with other important morphogenetic networks, such as Wnts and Fibroblast Growth Factors. The findings from this study provide insights into the role of Notch/Jagged1-mediated signaling in dental variations during evolution.
Review
Materials Science, Composites
Fereshte Rezaie, Masoud Farshbaf, Mohammad Dahri, Moein Masjedi, Reza Maleki, Fatemeh Amini, Jonathan Wirth, Keyvan Moharamzadeh, Franz E. Weber, Lobat Tayebi
Summary: Revolutionary fabrication technologies like 3D printing are expected to replace traditional methods in dental structure development due to their ability to create constructs with required properties and detailed structures. This review article explores the recent applications of 3D printing techniques in dental prosthetics manufacturing, including various printing techniques, materials used, and limitations/challenges faced. The article also utilizes major scientific databases to find relevant literature on dental prostheses 3D printing.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kevin Hofpeter, Matthias Zehnder, Michael Huelsmann, Anas Al-Jadaa, Shengjile Deari
Summary: This study aimed to develop a standardized curved root canal model in bovine dentine and compare its behavior to resin in standard plastic training blocks during chemo-mechanical preparation. The study found that dentine had higher microhardness compared to resin, and dentine exhibited greater canal transportation, which was slightly increased by EDTA. The results are important for testing and comparing materials and concepts of chemo-mechanical root canal instrumentation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Anamaria Balic, Dilara Perver, Pierfrancesco Pagella, Hubert Rehrauer, Bernd Stadlinger, Andreas E. Moor, Viola Vogel, Thimios A. Mitsiadis
Summary: In this study, a comprehensive single-cell atlas of carious human teeth was generated using single-cell RNA sequencing analysis and immunostaining. The study found modifications in various cell clusters within the carious dental pulp, including immune cells, mesenchymal stem cells, and fibroblasts. These modifications led to an increase in extracellular matrix components and myofibroblast enrichment. The study also revealed molecular, cellular, and biomechanical alterations in the carious dental pulp, indicative of extensive extracellular matrix remodeling.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2023)