Article
Biochemistry & Molecular Biology
Tao Li, Yu Chen, Yuan Li, Gang Chen, Yuqing Zhao, Guangyue Su
Summary: In this study, it was found that 20(R)-25-methoxyl-dammarane-3 beta,12 beta,20-triol (AD-1) can alleviate pulmonary fibrosis (PF) both in vitro and in vivo. The underlying mechanism may involve decreasing extracellular matrix (ECM) deposition and inflammation, enhancing antioxidant capacity, and regulating lung cell apoptosis and the TGF-beta 1/TIMP-1/alpha-SMA signaling pathway.
Article
Pharmacology & Pharmacy
Coralie Mergault, Fanny Lisee, Victor Tiroille, Melia Magnien, Christelle Parent, Woodys Lenga Mabonda, Damien Sizaret, Madeleine Jaillet, Bruno Crestani, Sylvain Marchand-Adam, Laurent Plantier
Summary: The study found that the expression of the Arp2/3 complex was increased in profibrotic environments in vitro and in vivo. Inhibition of the Arp2/3 complex repressed ACTA2 and COL1 expression and blocked the Akt/phospho-GSK3 beta/beta-catenin/MRTF-A pathway in lung fibroblasts. CK666 demonstrated antifibrotic properties in the lung in vivo.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yuko Ishida, Yumi Kuninaka, Naofumi Mukaida, Toshikazu Kondo
Summary: Fibrosis and structural remodeling of lung tissue can seriously impact lung function and have fatal consequences. The causes of pulmonary fibrosis (PF) vary, including allergens, chemicals, radiation, and environmental particles. The cause of idiopathic PF (IPF), one of the most common forms, is still unknown. The murine bleomycin (BLM) model has been extensively studied to understand the mechanisms of PF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuhua Liu, Shaofang Wang, Xueqi Gong, Yingshuo Wang, Tonghui Xu
Summary: Research indicates that B7, a B chain of recombinant human relaxin, effectively inhibits bleomycin-induced pulmonary inflammation, improving damaged alveolar structure, reducing collagen deposition, and suppressing primary pathological features of idiopathic pulmonary fibrosis.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lan Wang, Kai Xu, Ningdan Wang, Linke Ding, Wenyu Zhao, Ruyan Wan, Weiming Zhao, Xiaoshu Guo, Xin Pan, Juntang Yang, Ivan Rosas, Guoying Yu
Summary: This study demonstrates that fenbendazole (FBZ) treatment has an inhibitory effect on bleomycin-induced lung fibrosis in mice, as well as inhibiting the proliferation and migration of human embryo lung fibroblasts in vitro. The mechanism underlying this effect involves inhibition of glucose consumption, moderation of glycolytic metabolism, activation of adenosine monophosphate-activated protein kinase (AMPK), and reduction of the activation of the mammalian target of rapamycin (mTOR) pathway, ultimately leading to the inhibition of fibroblast-to-myofibroblast differentiation and collagen synthesis induced by transforming growth factor-beta (TGF-beta 1).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Aude Angelini, JoAnn Trial, Alexander B. Saltzman, Anna Malovannaya, Katarzyna A. Cieslik
Summary: The study focuses on the impaired myofibroblast development in aging male heart due to stiffer extracellular matrix (ECM), and shows decreased actin polymerization, α-smooth muscle actin (α-SMA), and Kindlin-2 expressions in old male cardiac fibroblasts. Manipulation of Kindlin-2 levels reversed the defective phenotype, and inhibition of overactivated ERK1/2 rescued actin polymerization and α-SMA expression. However, old female cardiac fibroblasts retained normal mechanosensing pathway.
Article
Immunology
Xiaohe Li, Ling Ma, Yuli Wei, Jinying Gu, Jingjing Liang, Shimeng Li, Yunyao Cui, Rui Liu, Hui Huang, Cheng Yang, Honggang Zhou
Summary: Cabozantinib shows potential as a treatment for acute lung injury and pulmonary fibrosis by improving pathological state, reducing inflammation, inhibiting fibrosis, and regulating key signaling pathways such as TLR4/NF-kappa B/NLRP3 and TGF-131/Smad3.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Respiratory System
Shengren Song, Zhenli Fu, Ruijuan Guan, Jie Zhao, Penghui Yang, Yang Li, Hang Yin, Yunxin Lai, Gencheng Gong, Simin Zhao, Jiangtian Yu, Xiaomin Peng, Ying He, Yumei Luo, Nanshan Zhong, Jin Su
Summary: This study investigated the dynamic features of bleomycin-treated mouse lungs with fibrosis using a combination of the latest technologies and traditional methods. The results showed that while the total lung hydroxyproline contents reached a peak at 4 weeks after bleomycin injury, other fibrosis evaluation parameters indicated a gradual degradation of collagens and a recovery of lung aeration. The data from immunostaining and fluorescent peptide staining explained this inconsistency, showing that hydroxyproline-rich degraded collagen fragments were detained and enriched within the intracellular regions at 10 or 16 weeks after bleomycin treatment.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Immunology
Yang Miao, Xiaohe Li, Yue Yang, Jianwei Zhang, Li Chen, Qianyi Zhang, Wenqi Li, Ying Liu, Xianfeng Zhang, Ruimin Gu, Cheng Yang
Summary: In this study, it was found that Entrectinib effectively inhibits the development of pulmonary fibrosis by blocking TGF-β1 signaling, both in vitro and in vivo.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Physiology
Julie M. M. Lade, Manuella R. R. Andrade, Clark Undem, Jasmine Walker, Haiyang Jiang, Xin Yun, Larissa A. A. Shimoda
Summary: Exposure to hypoxia leads to structural changes in pulmonary vascular wall, including hyperplasia and migration of pulmonary arterial smooth muscle cells (PASMCs). In this study, the interaction between ezrin and NHE1 was found to increase under hypoxic conditions, promoting SMA binding and enhancing PASMC migration and proliferation.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Katherine A. Cummins, Peter B. Bitterman, Daniel J. Tschumperlin, David K. Wood
Summary: Idiopathic pulmonary fibrosis (IPF) is a deadly lung disease with a lack of high-fidelity models for therapeutic development. Researchers have successfully created a three-dimensional (3D) platform to study lung fibrosis, demonstrating enhanced predictability and fidelity compared to 2D systems and superior tractability and throughput compared to 3D systems, using normal lung fibroblasts and fibroblasts from IPF patient lungs.
APL BIOENGINEERING
(2021)
Review
Cell & Tissue Engineering
Hesham Soliman, Marine Theret, Wilder Scott, Lesley Hill, Tully Michael Underhill, Boris Hinz, Fabio M. Rossi
Summary: MSCs are essential for the development, maintenance, function, and regeneration of most tissues. They play a crucial role in responding to injury, promoting regeneration through secretion of trophic factors and ECM molecules, and contributing to fibrotic reparative processes. Through fate mapping and single cell omics, tissue-specific MSC identities and functions are being elucidated, providing unprecedented insights into the secret lives of tissue-resident MSCs.
Article
Biochemistry & Molecular Biology
Basalova Nataliya, Arbatskiy Mikhail, Popov Vladimir, Grigorieva Olga, Vigovskiy Maksim, Zaytsev Ivan, Novoseletskaya Ekaterina, Sagaradze Georgy, Danilova Natalia, Malkov Pavel, Cherniaev Andrey, Samsonova Maria, Karagyaur Maxim, Tolstoluzhinskaya Anastasiya, Dyachkova Uliana, Akopyan Zhanna, Tkachuk Vsevolod, Kalinina Natalia, Efimenko Anastasiya
Summary: Membrane-bound vesicles released by fat-derived stem cells contain small RNA molecules that help resolve lung scarring. These molecules cause scar-forming cells to become more flexible, leading to healthier lung function. Drugs targeting these molecules could potentially treat fibrosis in various organs.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Fatmah Al-Habeeb, Noof Aloufi, Hussein Traboulsi, Xingxing Liu, Parameswaran Nair, Christina Haston, Ilan Azuelos, Steven K. Huang, Eric S. White, Imed E. Gallouzi, Sergio Di Marco, David H. Eidelman, Carolyn J. Baglole
Summary: The study revealed the significant role of HuR in promoting ECM production, myofibroblast differentiation, and lung fibrosis. Understanding the function of HuR could potentially lead to the development of new targeted therapy for fibrotic diseases such as Idiopathic Pulmonary Fibrosis (IPF).
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiang Tong, Shijie Zhang, Dongguang Wang, Li Zhang, Jizheng Huang, Tianli Zhang, Hong Fan
Summary: Azithromycin (AZM) effectively attenuates BLM-induced pulmonary fibrosis in mice by partially inhibiting the JNK/c-Jun and TGF-beta 1/Smad signaling pathways and reducing production of LOX and LOXL2.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Nutrition & Dietetics
Po-An Hu, Chia-Hui Chen, Bei-Chia Guo, Yu Ru Kou, Tzong-Shyuan Lee
Article
Physiology
Hsin-Kuo Ko, An-Hsuan Lin, Diahn-Warng Perng, Tzong-Shyuan Lee, Yu Ru Kou
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Chung-Kuan Wu, Chia-Lin Wu, Tzong-Shyuan Lee, Yu Ru Kou, Der-Cherng Tarng
Summary: The study found that TRPA1 plays a role in AKI by sensing oxidative stress and regulating inflammation in renal tubular cells. TRPA1 expression increased in patients with AKI and in a mouse model of IR-induced injury, highlighting its importance in kidney injury. In vitro experiments showed that TRPA1 activation by ROS under hypoxia-reoxygenation injury conditions led to increased intracellular Ca2+ level and activation of inflammatory signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Chung-Kuan Wu, Ji-Fan Lin, Tzong-Shyuan Lee, Yu Ru Kou, Der-Cherng Tarng
Summary: TRPA1 plays dual roles in sensory neurons and non-neuronal cells, promoting tissue repair, clearing harmful stimuli, and inducing protective responses, while also potentially contributing to disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Pei-Chen Tsao, Chih-Hsueh Lin, Yu-Sheng Lee, Wei-Yu Chen, Mei-Jy Jeng, Yu Ru Kou
Summary: Intratracheal budesonide delivered by full-strength surfactant provides optimal protection against LPS-induced ALI in surfactant-insufficient rat lungs, as evidenced by improved gas exchange, reduced lung injury scores, and lower levels of inflammatory markers compared to other treatment groups.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2021)
Article
Biochemistry & Molecular Biology
Man-Chen Hsu, Wei-Jia Luo, Bei-Chia Guo, Chia-Hui Chen, Po-An Hu, Yi-Hsuan Tsai, Kang-Yi Su, Tzong-Shyuan Lee
Summary: In this study, the role of HLJ1 in blood coagulation was investigated using wild-type mice and HLJ1-null mice. The results suggest that although HLJ1 is present in megakaryocytes and platelets, it may not play a role in blood coagulation under normal physiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Wen-Kuang Yu, Yu-Chun Chen, Wei-Chih Chen, Vincent Yi-Fong Su, Kuang-Yao Yang, Yu Ru Kou
Summary: Low GCS and high initial RSBI are two independent factors that synergistically affect tracheostomy in patients with SCI at C3-C5. These findings can assist in making decisions regarding tracheostomy in this specific patient population.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2022)
Article
Nutrition & Dietetics
Po-An Hu, Sz-Han Wang, Chia-Hui Chen, Bei-Chia Guo, Jenq-Wen Huang, Tzong-Shyuan Lee
Summary: Bromelain has anticoagulant effects on non-alcoholic fatty liver disease-induced blood coagulation deregulation by inhibiting the activation of the coagulation cascade, decreasing the stability of clots, and promoting fibrinolytic activity. The findings suggest the potential therapeutic value of bromelain for thrombosis-related diseases.
Article
Medicine, General & Internal
Yi-Han Hsiao, Yu-Jung Lin, Tien-Hsin Jeng, Kang-Cheng Su, Hsin-Kuo Ko, Sheau-Ning Yang, Diahn-Warng Perng, Yu Ru Kou
Summary: This study investigated the potential of impulse oscillometry (IOS) in evaluating bronchodilator reversibility (BDR) in untreated adult patients with newly diagnosed asthma (UAPNDS). The results showed that Delta Ax (%), an IOS parameter, could serve as a novel indicator to evaluate BDR in UAPNDS patients.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Chia-Hui Chen, Chien-Chung Hsia, Po-An Hu, Chung-Hsin Yeh, Chun-Tang Chen, Cheng-Liang Peng, Chih-Hsien Wang, Tzong-Shyuan Lee
Summary: Bromelain, a cysteine protease found in pineapple, has been found to have beneficial effects in the treatment of inflammatory diseases. In this study, the effects of bromelain on hyperlipidemia and atherosclerosis in mice were investigated. It was found that daily administration of bromelain improved hyperlipidemia, reduced aortic inflammation, and slowed down the progression of atherosclerosis. The mechanisms underlying these effects involve the promotion of cholesteryl ester hydrolysis, autophagy, and antioxidant capacity through the AMPK/TFEB signaling pathway.
Article
Biochemistry & Molecular Biology
Ko-Lin Kuo, Chin-Wei Chiang, Yi-Ming Arthur Chen, Chih-Chin Yu, Tzong-Shyuan Lee
Summary: Folic acid has anti-inflammatory and antifibrotic effects. The major folic acid-binding protein in the liver, GNMT, regulates cellular methylation and may be a therapeutic target in treating renal fibrosis. Increased expression of GNMT was observed in humans with obstructive nephropathy and in mice with unilateral ureteral obstruction. Folic acid treatment alleviated renal injury in wild-type mice, but not in GNMT knockout mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tsung-Ming Chang, Yao-Chang Chiang, Chiang-Wen Lee, Chieh-Mo Lin, Mei-Ling Fang, Miao-Ching Chi, Ju-Fang Liu, Yu Ru Kou
Summary: This study reveals the function of CXCL14 in promoting EMT and metastasis in lung cancer, with ACKR2 playing a role in this process. These findings can be used as prognostic biomarkers of lung cancer metastasis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Oncology
Kun-Ming Wu, Chih-Wen Chi, Jerry Cheng-Yen Lai, Yu-Jen Chen, Yu Ru Kou