Article
Horticulture
Huixia Jia, Jiangping Song, Wenlong Yang, Xiaohui Zhang, Yue Zhu, Danqi Zhao, Haiping Wang
Summary: Garlic is an essential ingredient and has health benefits. AP2/ERF transcription factors play important regulatory roles in garlic breeding, but their identification and characterization in garlic are not well understood. In this study, 163 AsAP2/ERFs were identified in the garlic genome and categorized into four subfamilies. Gene structural analysis showed that most AsERFs and all AsRAVs were intronless, while most AsAP2s had introns. Gene duplications were observed and cisacting elements associated with development, hormone signaling, and stress responses were detected in promoter regions. Tissue-specific expression profiles and co-expression network analysis revealed the potential roles of AsAP2/ERFs in bulb and bolt development in garlic.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Qianlong Yu, Minghui Wang, Xuemeng Ding, Jiachen Han, Hancheng Ma, Jie Li, Guiling Zheng, Bin Zhang, Changyou Li
Summary: Different Spodoptera exigua cell lines showed different apoptosis responses during Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infection. The activation of SeCaspase-1 to -6 and the inhibition of iap1, iap2, and p35 in Se-1 cells contributed to severe apoptosis, while the opposite occurred in Se-3 cells. The different expression of P35 was identified as an important reason for the apoptosis differences between the two cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yuying Han, Xiaoliang Gao, Nan Wu, Yirong Jin, He Zhou, Weijie Wang, Hao Liu, Yi Chu, Jiayi Cao, Mingzuo Jiang, Suzhen Yang, Yanting Shi, Xin Xie, Fulin Chen, Ying Han, Wen Qin, Bing Xu, Jie Liang
Summary: Ferroptosis, a regulated cell death induced by iron-dependent lipid peroxidation, is important in tumor development and drug resistance. In this study, we found that LINC00239 is significantly overexpressed in colorectal cancer (CRC) tissues and its high expression predicts poorer survival and prognosis in CRC patients. We also demonstrated that LINC00239 inhibits ferroptosis, decreasing the anti-tumor activity of certain drugs. Importantly, LINC00239 interacts with Keap1 to regulate ferroptosis, suggesting a potential therapeutic strategy for CRC patients with low LINC00239 expression.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Maryam Pourhajibagher, Shahroo Etemad-Moghadam, Mojgan Alaeddini, Abbas Bahador
Summary: This study synthesized a novel nanomaterial NET and investigated its anti-cancer effects on head and neck squamous cell carcinoma cell lines in combination with ultrasound. The results demonstrated that NET-SDT can significantly enhance apoptosis and reduce cell viability, indicating a potential anti-cancer role in HNSCC cells.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
Article
Genetics & Heredity
Shijun Yang, Caixia Tan, Xuerong Sun, Xiong Tang, Xiao Huang, Fan Yan, Guangxiang Zhu, Qin Wang
Summary: This study investigates the mechanisms of metamorphosis in the Chinese giant salamander, focusing on the role of caspase genes and the effect of thyroid hormone treatment. The results show that caspase genes play a key role in the loss of external gills, and thyroid hormone treatment can accelerate this process.
Article
Plant Sciences
Mikhail A. Filyushin, Olga K. Anisimova, Anna V. Shchennikova, Elena Z. Kochieva
Summary: In this study, the DREB A1 and A2 genes involved in plant stress responses were identified and characterized in garlic. The expression patterns of these genes were analyzed in garlic cultivars with different sensitivity to cold and Fusarium infection. The results showed that these genes had different responses to fungal infection and cold exposure, and could be potential targets for breeding stress-tolerant varieties.
Article
Plant Sciences
Pranjali A. Gedam, Kiran Khandagale, Dhananjay Shirsat, A. Thangasamy, Onkar Kulkarni, Abhijeet Kulkarni, Swaranjali S. Patil, Vitthal T. Barvkar, Vijay Mahajan, Amar Jeet Gupta, Kiran P. Bhagat, Yogesh P. Khade, Major Singh, Suresh Gawande
Summary: This study identified differentially expressed genes (DEGs) through transcriptome analysis in two onion genotypes (Acc. 1666 and W-344) with contrasting responses to waterlogging stress. The proteins coded by these DEGs regulate key biological processes such as phytohormone production, antioxidant enzymes, programmed cell death, and energy production. The results provide valuable molecular information for improving stress tolerance and developing waterlogging tolerant onion varieties.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Md. Ashraful Alam, Md. Atikur Rahman, Md. Marufur Rahman, Md. Mahmudul Hasan, Shamsun Naher, Abu Hena Faisal Fahim, Md. Abdul Mottalib, Srabanti Roy, Md. Rafiqul Islam, Shailendra Nath Mozumder, Amnah Mohammed Alsuhaibani, Ahmed Gaber, Akbar Hossain
Summary: Salt stress is a major obstacle in crop production, particularly for glycophytic crops like onions. Extensive research has been conducted to develop genotypes that are more resistant to salt stress. Multiple tolerant genotypes have been identified for mass production.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Oceanography
Arat Yanglang, Haishen Wen, Xuebin Mao, Yuan Tian, Lingyu Wang, Jinku Li, Xin Qi, Prapansak Srisapoome, Jifang Li, Yun Li
Summary: A study has identified a set of caspase genes in spotted sea bass that regulate apoptosis and contribute to various biological processes. The expression of these genes was found to be significantly altered after infection with Vibrio harveyi, particularly in the head kidney and spleen, suggesting their important role in immune regulation. This study provides a comprehensive overview of the caspase gene family in spotted sea bass and lays the foundation for further understanding their biological functions.
JOURNAL OF OCEAN UNIVERSITY OF CHINA
(2023)
Article
Multidisciplinary Sciences
Rui Meng, Junxian Song, Lina Guan, Qian Li, Cuige Shi, Dongmei Su, Xu Ma
Summary: This study used MethylRAD sequencing to investigate changes in methylation levels in the fetal heart genome in a rat model of hyperglycemia. The results showed that methylation was enriched in intergenic regions and genes related to abnormal CCGG/CCNGG methylation in the upstream region may play dominant roles in heart malformations. Further research on these genes may provide potential molecular targets or diagnostic biomarkers for heart diseases under hyperglycemia.
Article
Multidisciplinary Sciences
Madalena Chaves, Luis C. Gomes-Pereira, Jeremie Roux
Summary: This study developed a modeling approach utilizing single-cell response data to identify regulatory reactions driving population heterogeneity in drug response, providing potential regulatory targets to curb cell dynamics of drug resistance.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Xiaoyue Zhang, Kaixia Zhao, Lan Lan, Na Shi, Hao Nan, Yanan Shi, Xiaodong Xu, Hongying Chen
Summary: By designing gene cassettes to eliminate cell apoptosis, this study successfully increased protein expression efficiency and developed a novel baculovirus vector that could be widely applied in scientific research and pharmaceutical industries.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ikumi Nakashima, Shoji Saito, Eiichi Akahoshi, Shigeki Yagyu, Mitsuko Sugano-Ishihara, Yozo Nakazawa
Summary: Breast cancer is a complex disease with unique molecular subtypes. The combination of tumor-tropic lipid nanoparticles and inducible caspase-9 mRNA shows promise as a potential treatment strategy for refractory breast cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Neurosciences
Aziz Unnisa, Nigel H. H. Greig, Mohammad Amjad Kamal
Summary: Traumatic brain injury (TBI) is a major cause of death and morbidity, and is the focus of translational research. Apoptosis is important in the pathophysiology of TBI, and inhibiting it may improve functional recovery. Caspases, such as Caspase-3 and Caspase-9, are key mediators of cell death due to apoptosis in TBI. Understanding these apoptotic pathways and finding ways to inhibit Caspases may have significant treatment implications for TBI.
CURRENT NEUROPHARMACOLOGY
(2023)
Article
Biology
Sabah Ghasan Abood Al-Ashoor, Vasudevan Ramachandran, Liyana Najwa Inche Mat, Nur Afiqah Mohamad, Mohd Hazmi Mohamed, Wan Aliaa Wan Sulaiman
Summary: Type 2 Diabetes mellitus is a chronic metabolic disorder affecting a large population globally, with OCT3 (rs2292334) gene possibly being a genetic risk factor for the development of T2DM among Indian males.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mohamed Suhail Nawabjohn, Prathibha Sivaprakasam, Suresh Kumar Anandasadagopan, A. Adeela Begum, Ashok Kumar Pandurangan
Summary: Green synthesis of silver nanoparticles using Cassia tora seed extract showed strong antibacterial activity, confirming the successful formation of nanoparticles with a variety of characterization techniques.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Srinivetha Pathmanapan, Mythrehi Sekar, Ashok Kumar Pandurangan, Suresh Kumar Anandasadagopan
Summary: The study developed a composite nanofiber scaffold for bone tissue engineering using coaxial electrospinning technique, containing organic oregano extract, mesoporous silica nanoparticles, collagen, and hydroxyapatite. The scaffold showed good biocompatibility, cell adhesion, survival, and mineralization potential, making it a promising implantable biomaterial for bone repair.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Clinical Neurology
Nabil Izzaatie Mohamad Safiai, Nur Afiqah Mohamad, Hamidon Basri, Liyana Najwa Inche Mat, Fan Kee Hoo, Anna Misyail Abdul Rashid, Abdul Hanif Khan Yusof Khan, Wei Chao Loh, Janudin Baharin, Aaron Fernandez, Intan Nureslyna Samsudin, Mohd Hazmi Mohamed, Siew Mooi Ching, Kai Wei Lee, Vasudevan Ramachandran, Patricia Pozo-Rosich, Wan Aliaa Wan Sulaiman
Summary: High-frequency repetitive transcranial magnetic stimulation over dorsolateral prefrontal cortex may be effective in reducing acute medication intake and functional disability in migraine patients.
Review
Biochemistry & Molecular Biology
Ashwathi Vijayalekha, Suresh Kumar Anandasadagopan, Ashok Kumar Pandurangan
Summary: Bone regeneration is a complex physiological process that occurs throughout a person's life. Current bone repair methods have limitations, while tissue engineering offers potential for repairing damaged tissue. Collagen-based biomaterials, particularly collagen type I, play a crucial role in bone tissue engineering.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Genetics & Heredity
Neda Ansari, Vasudevan Ramachandran, Nur Afiqah Mohamad, Elnaz Salim, Patimah Ismail, Mohamad Hazmi, Liyana Najwa Inchee Mat
Summary: The objective of this study was to determine the association of candidate genetic polymorphisms of GCK (rs1799884), GCKR (rs780094), and G6PC2 (rs560887) genes with type 2 diabetes mellitus (T2DM) among Malay ethnics. The results showed significant differences in genotypic and allelic frequencies of GCK (rs1799884), GCKR (rs780094), and G6PC2 (rs560887) gene polymorphisms between T2DM and control subjects.
GLOBAL MEDICAL GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Murali Santhoshkumar, Dhandapani Perumal, Jayaraman Narenkumar, Vasudevan Ramachandran, Karnan Muthusamy, Ahmed Alfarhan, Ernest David
Summary: This study demonstrated that bio-functional silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) synthesized from Gymnema sylvestre leaves showed potent toxicity against triple-negative breast cancer cells (TNBC). The characterization of the nanoparticles was performed using various techniques, and their anti-cancer effects were evaluated using in vitro cytotoxicity studies. The results indicated that the phytofabricated nanoparticles exhibited excellent prospects for anti-cancer activity in the pharmaceutical and medical fields.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Medicine, General & Internal
Siew Mooi Ching, Rajvinder Singh, Fatimah Shahidah Binti Azmi, Khian Lu Chong, Cynthia Ruey Shyuan Ong, Nur Afiqah Binti Ayob, Mansi Patil, Kai Wei Lee, Vasudevan Ramachandran, Viresh Arora
Summary: This study aimed to determine the prevalence, socio-demographic characteristics, and factors associated with probable obstructive sleep apnea (OSA) in hypertensive patients in Sarawak, Malaysia. The results showed a high prevalence rate of probable OSA (54.4%) among hypertensive patients, with significant positive associations found with smoking, retirees, and being of Chinese ethnicity. Primary care physicians should be vigilant in identifying hypertensive patients at risk for OSA, as early detection and intervention can reduce disease complications and healthcare costs.
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Review
Chemistry, Analytical
Pooja Chinnikrishnan, Ibrahim Abdel Aziz Ibrahim, Abdullah R. Alzahrani, Naiyer Shahzad, Prathibha Sivaprakasam, Ashok Kumar Pandurangan
Summary: Triple-negative breast cancer (TNBC) lacks receptors for estrogen, progesterone, and human epidermal growth factor, making its treatment challenging. Developing chemotherapeutic drugs using flavonoids from natural products, such as blueberries and onions, may be a potential solution due to their increased efficacy and reduced side effects. Flavonoids have shown anticancer properties and the ability to modulate tumor cell activities, making them a promising option for TNBC treatment.
Article
Psychiatry
Asieh Kouhi Fayegh, Hazwan Mat Din, Wan Aliaa Wan Sulaiman, Maryam Ravanipour, Hamidon Basri, Mohd Hazmi Bin Mohamed, Vasudevan Ramachandran, Liyana Najwa Inche Mat
Summary: This study compared sleep quality, chronotype, and obstructive sleep apnea risk between elderly people with and without migraine. The results showed that elderly migraine sufferers had higher scores on the global Pittsburgh Sleep Quality Index, but there was no significant relationship between chronotype and obstructive sleep apnea risk and migraine.
Article
Medicine, General & Internal
Siew-Mooi Ching, Ai Theng Cheong, Anne Yee, Ramayah Thurasamy, Poh Ying Lim, Irmi Ismail Zarina, Kai Wei Lee, Sri Wahyu Taher, Vasudevan Ramachandran
Summary: This study assessed the determinants of burnout among healthcare providers in the primary care setting. The prevalence of personal burnout was 41.7%, work-related burnout was 32.2%, and client-related burnout was 14.5%. Factors contributing to personal burnout included younger age, being a doctor, higher COVID-19 exposure risk, inability to handle stress, poorer sleep quality, higher COVID-19 fear and stress scores, and lower brief resilience score (BRS). Factors contributing to work-related burnout included younger age, being a doctor, longer years of working, higher COVID-19 exposure risk, inability to handle stress, lower altruistic score, poorer sleep quality, higher stress scores, and lower BRS score. Factors contributing to client-related burnout included being a doctor, being single/divorced, having more than one attachment site, higher satisfaction with infection control, inability to handle stress, higher depression scores, and lower BRS score.
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Food Science & Technology
Srinivasan Kumaraswamy, Geetha Arumugam, Ashok Kumar Pandurangan, Vasantha Srinivasan Prabhakaran, Radhakrishnan Narayanaswamy
Summary: Organic acids in honey can regulate the availability and absorption of non-heme iron. In this study, 53 selected honey ligands were assessed for their docking behavior on human Ferritin, Transferrin, and Hepcidin. Molecular physicochemical and ADME analyses were conducted, revealing the potential effects of these ligands. The findings highlight the potential of honey ligands in modulating human Ferritin, Transferrin, and Hepcidin.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sekar Harikrishnan, Shanmugam Sudarshan, Kandasamy Sivasubramani, M. S. Nandini, Jayaraman Narenkumar, Vasudevan Ramachandran, Bader O. Almutairi, Paulraj Arunkumar, Aruliah Rajasekar, Singaram Jayalakshmi
Summary: The widespread use of synthetic pesticides has led to various issues, necessitating the development of new microbial derived insecticides for safe and environmentally-friendly pest control. This study evaluated the toxicity of a biosurfactant produced from Enterobacter cloacae on mosquito larvae and termites, finding that it exhibited larvicidal and anti-termite activity. The biosurfactant caused substantial tissue damage in the studied organisms. These findings suggest that the microbial biosurfactant has great potential for the control of mosquitoes and termites.
SCIENTIFIC REPORTS
(2023)
Article
Toxicology
Sobia Nida, Hemalatha Srinivisan, Ashok Kumar Pandurangan, Mohammad Waseem
Summary: The emergence of SARS-CoV-2 has spread rapidly and caused significant impact on human population. Along with its respiratory effects, this virus has also been associated with neurological complications. This review focuses on COVID-19 mediated neurodegeneration, its impact on affected patients, and discusses potential therapeutic interventions and experimental models for drug and vaccine development against COVID-19 mediated neurological complications.
JOURNAL OF ENVIRONMENTAL PATHOLOGY TOXICOLOGY AND ONCOLOGY
(2022)
Article
Genetics & Heredity
Elnaz Salim, Vasudevan Ramachandran, Neda Ansari, Patimah Ismail, Mohd Hazmi Mohamed, Nur Afiqah Mohamad, Liyana Najwa Inche Mat
Summary: The T allele of ET-1 rs5370 and the C allele of ECE rs212526 are significant genetic risk factors for EH among Malay subjects. Additionally, BMI and age are associated with hypertension.