Journal
CHEMELECTROCHEM
Volume 4, Issue 7, Pages 1768-1774Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.201700035
Keywords
Electrochemiluminescence; aptasensors; Au-g-C3N4 NH; acetylcholinesterase; label free
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Funding
- National Natural Science Foundation of China [21575001]
- Natural Science Foundation of Anhui Province [1508085MB37]
- Key Project of Anhui Provincial Education Department [KJ2014A016]
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A feasible label-free electrochemiluminescence (ECL) aptasensor that uses an Au-nanoparticle-functionalized g-C3N4 nanohybrid (Au-g-C3N4 NH) as the luminophore was constructed for highly sensitive acetylcholinesterase (AChE) detection. The sensor was fabricated by successively modifying a glassy carbon electrode with Au-g-C3N4 NH and thiol-modified AChE-specific aptamers. In the presence of AChE, the ECL signal decreased significantly, because AChE could hydrolyze the substrate acetylthiocholine to generate acetic acid, which could react with the co-reactant triethylamine (Et3N), leading to evident consumption of the co-reactant. The ECL response of the aptasensor was linearly proportional to the concentration of AChE ranging from 0.1 pg/mL to 10 ng/mL, with a detection limit of 42.3 fg/mL (S/N= 3). This novel ECL sensing strategy demonstrated a highly sensitive and selective method for AChE detection and was expected to possess potential applications in clinical diagnosis and biomedical technology.
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