Review
Chemistry, Applied
Kwon-Young Choi
Summary: Indigo dyeing technology generates hazardous by-products and sludge, causing environmental pollution. Biological treatments using enzymes have been spotlighted as a green solution, but process design and enzyme production strategy must be optimized for cost-effectiveness. Furthermore, attention should be given to electron mediators supply and unexpected by-products removal during the whole process.
Article
Biochemistry & Molecular Biology
Chunlei Wang, Sijia Wang, Jieru Zhang, Shumin Jiang, Daizong Cui, Haiqiong Sun, Chengwei Liu, Lili Li, Min Zhao
Summary: This article investigates the capacity of Bacillus safensis HL3 spore to degrade and detoxify indigo carmine. The spore is found to effectively decolorize indigo carmine within 2 hours and increase the activities of laccase, intracellular tyrosinase, and lignin peroxidase. The study proposes a detailed pathway of indigo carmine degradation by bacterial spores and demonstrates the reduced toxicity of the biodegradation products.
Article
Biotechnology & Applied Microbiology
Aakanksha Ahlawat, Avijeet S. Jaswal, Saroj Mishra
Summary: The addition of indigo carmine enhances the growth of the white-rot fungus Cyathus bulleri, particularly on malt-extract agar and in potato dextrose broth. The indigo carmine is degraded into anthranilic acid by oxidoreductases, specifically laccases, in the fungus culture broth. The anthranilic acid then enters the tryptophan biosynthetic pathway.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Materials Science, Multidisciplinary
R. Shakunthala, T. Kavya Krishnaveni, Manickam Matheswaran
Summary: A non-metal type photocatalytic layered boron nitride (BN) material was synthesized by hydrothermal process and pyrolysis. The synthesized BN exhibited a hexagonal crystal structure with a strong B--N bond. The photocatalytic decolorization of indigo carmine (IC) dye was achieved with a 88% efficiency using the synthesized BN under UV light. Response surface methodology (RSM) based box-benken design (BBD) was used to optimize the operating parameters and a model equation for decolorization efficiency was obtained. The photocatalytic decolorization mechanism was proposed based on experimental results.
Article
Chemistry, Physical
Sonia Ben Younes, Chadia Dallali, Ali Ellafi, Lamjed Bouslama, Anwar Feriani, Sami Sayadi
Summary: The study characterized eleven bacterial strains isolated from various Tunisian biotopes for their lignin degradation activities and screened them for their ability to decolorize Indigo Carmine. Results showed that the bacterial strains were effective in decolorizing Indigo Carmine, with some strains also displaying potential detoxification capabilities. The study suggests that these bacterial isolates could be utilized as powerful tools for bioremediation of textile effluents and other potential applications.
Article
Biotechnology & Applied Microbiology
Na Yan, Hui Ma, Chun-Xue Yang, Xiang-Ru Liao, Zheng-Bing Guan
Summary: In this study, Bacillus pumilus W3 CotA-laccase was engineered to enhance its decolorization activity towards Congo Red (CR) through rational modification of three amino acids in the substrate-binding pocket. The obtained multi-site mutants showed significantly improved decolorization efficiency and catalytic efficiency compared to the wild-type CotA. Structural analysis suggested possible mechanisms for the activity enhancement of the mutants, indicating their potential as novel bioremediation tools.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Youri Yang, Sunil Ghatge, Hor-Gil Hur
Summary: Directed evolution was used to enhance the laccase activity of thermoalkaliphilic laccase. The V243D mutant showed increased enzyme activity and catalytic efficiency compared to the wild-type. Real-time oxygen measurement technique proved to be useful in evaluating oxidase activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Navleen Kaur Chopra, Sonica Sondhi
Summary: The laccase gene from Bacillus licheniformis NS2324 was cloned and expressed in E. coli, and its properties and activities were studied. The results showed that the recombinant laccase exhibited good tolerance and decolorization ability, making it suitable for eco-friendly and cost-effective dye decolorization in industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Krzysztof Michalski, Christian Hertig, Dariusz R. Mankowski, Jochen Kumlehn, Janusz Zimny, Anna M. Linkiewicz
Summary: Cas endonuclease-mediated genome editing offers a long-awaited molecular biological approach for modifying predefined genomic target sequences in living organisms. The implementation of this technology can be challenging for species like triticale, but pre-validated cas9/gRNA constructs provide a strong foundation for the targeted induction of heritable modifications in triticale genes. The study also demonstrates that in-del induction frequency in triticale can be increased by TREX2 nuclease activity, regardless of gRNA performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jiangbo Zhan, Hengsong Sun, Zhuojun Dai, Yi Zhang, Xiaorong Yang
Summary: “”In this study, the function of two amino acid residues, D357 and K430, in Thermus thermophilus SG0.5JP17-16 laccase (lacTT) were investigated. Mutations of these residues led to changes in the catalytic efficiency and decolorization ability of the enzyme, providing insights for understanding and improving the functionality of laccase.
Article
Biochemistry & Molecular Biology
Tao Li, Hongbin Wang, Jingwen Li, Luying Jiang, Hongwei Kang, Zehui Guo, Chen Wang, Wei Yang, Fufeng Liu, Fuping Lu, Yihan Liu
Summary: A newly isolated laccase gene from Bacillus licheniformis TCCC 111219 was expressed efficiently in Escherichia coli, showing high stability towards various pH levels and temperatures. However, simultaneous exposure to strong alkaline and high temperature environments resulted in significant damage to the active site loop regions of the enzyme. This thermo- and alkaline-stable enzyme demonstrated efficient decolorization of structurally different dyes, indicating its significant potential for industrial applications in textile dyeing effluent treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Octavian Dumitru Pavel, Alexandra-Elisabeta Stamate, Rodica Zavoianu, Anca Cruceanu, Alina Tirsoaga, Ruxandra Birjega, Ioana Andreea Brezestean, Alexandra Ciorita, Daniela Cristina Culita, Ana Paula Soares Dias
Summary: This paper focuses on the use of hybrid catalysts (Mo-LDH-GO composites) for the removal of indigo carmine dye (IC) from wastewater using environmentally friendly H2O2 as the oxidation agent. The catalysts were characterized and the most efficient catalyst was found to be the one with 20%wt. GO, achieving a removal rate of 96.6% for IC. The results suggest a strong correlation between catalytic activity, textural properties, and basicity of the catalysts.
Article
Biochemistry & Molecular Biology
Yitong Jia, Qianqian Huang, Lanlan Zhu, Chengyuan Pan
Summary: Trametes hirsuta secretes laccase isoenzymes, and an inducible laccase from this species was successfully expressed in Pichia pastoris. The recombinant laccase showed optimal pH and temperature for the oxidation of ABTS, and its activity was influenced by metal ions and organic solvents. Furthermore, the laccase exhibited efficient decolorization ability for certain dyes, suggesting its potential application in dye removal from wastewater.
Article
Multidisciplinary Sciences
Liubov Igorevna Trubitsina, Azat Vadimovich Abdullatypov, Anna Petrovna Larionova, Ivan Vasilyevich Trubitsin, Sergey Valerievich Alferov, Olga Nikolaevna Ponamoreva, Alexey Arkadyevich Leontievsky
Summary: A novel two-domain laccase CjSL was identified, cloned, expressed, and characterized in this study, showing excellent oxidation performance and stability, which holds potential value in biotechnological applications.
Article
Polymer Science
Saerom Park, Dahun Jung, Hyejin Do, Jonghyeon Yun, Dongjun Lee, Soeun Hwang, Sang Hyun Lee
Summary: This study developed a laccase-mediator system using natural mediators for melanin decolorization, effectively overcoming issues such as the low redox potential of laccase and limited access of melanin to the active site. Natural mediators with methoxy and ketone groups played a crucial role in melanin decolorization, and using acetosyringone as a mediator significantly enhanced the specificity constants of laccases. The laccase-mediator system could decolorize synthetic eumelanin analogs and natural melanin produced by melanoma cells, showing promising potential for skin whitening applications.