Journal
BOTANICAL STUDIES
Volume 58, Issue -, Pages -Publisher
SPRINGEROPEN
DOI: 10.1186/s40529-017-0214-6
Keywords
Conservation; Gastrodia; Leaf litter-decomposing fungi; Mycoheterotrophic plants
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Funding
- JSPS KAKENHI [17K07536, 15H04417]
- Research Grant for Plant Science from The New Technology Development Foundation
- National Museum of Nature and Science research grant entitled Integrated analysis of natural history collections for conservation of highly endangered species
- Grants-in-Aid for Scientific Research [17K07536, 15H04417] Funding Source: KAKEN
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Background: Mycoheterotrophic plants are one of the most difficult plant groups to conserve because they are entirely dependent on symbiotic fungi. Establishment of viable culture systems would greatly aid their conservation. We describe a simple culture system for the mycoheterotrophic orchid, Gastrodia pubilabiata, that does not require laboratory facilities. The orchid is symbiotic with leaf-litter-decomposing fungi. Results: Gastrodia pubilabiata seeds were incubated in plastic boxes or glass bottles filled with leaf litter collected from the natural habitat of the species. Seed germination was observed after 35 days and seedling development followed. Fungal isolates from seedlings were identified as Mycenaceae (Basidiomycota), a leaf-litter-decomposing mycorrhizal fungus of Gastrodia species. Conclusion: Our method can be used to conserve endangered mycoheterotrophic plants associated with leaf litterdecomposing fungi efficiently, and can also serve as a model system for physiological and molecular studies of such plants.
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