Journal
BIOLOGY OPEN
Volume 6, Issue 9, Pages 1364-1374Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.024927
Keywords
Short bowel syndrome; Ileocecal resection; Intestinal adaptation; Bile acid; Vitamin B12
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT KAKENHI) [26112705]
- Japan Society for the Promotion of Science London (JSPS KAKENHI) [24390186, 26221307]
- Regenerative Medicine Realization Base Network Program from the Japan Agency for Medical Research and Development
- Ministry of Health, Labor and Welfare of Japan
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [17K19674, 26112705, 26221307, 24390186] Funding Source: KAKEN
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Ileocecal resection (ICR), one of several types of intestinal resection that results in short bowel syndrome (SBS), causes severe clinical disease in humans. We here describe a mouse model of massive ICR in which 75% of the distal small intestine is removed. We demonstrate that mice underwent 75% ICR show severe clinical signs and high mortality, which may recapitulate severe forms of human SBS, despite an adaptive response throughout the remnant intestine. By using this model, we also investigated whether the epithelium of the remnant intestine shows enhanced expression of factors involved in region-specific functions of the ileum. Cubn mRNA and its protein product, which play an essential role in vitamin B12 absorption in the ileum, are not compensatory up-regulated in any part of the remnant intestine, demonstrating a clear contrast with post-operative up-regulation of genes involved in bile acid absorption. Our study suggests that functional adaptation by phenotypical changes in the intestinal epithelium is not a general feature for nutrient absorption systems that are confined to the ileum. We also propose that the mouse model developed in this study will become a unique system to facilitate studies on SBS with ICR in humans.
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