Article
Agriculture, Multidisciplinary
Xiaoxian He, Xuena Guo, Zhengda Du, Xuelian Liu, Junnian Jing, Chenyao Zhou, Yanfei Cheng, Zhaoyue Wang, Xiu-Ping He
Summary: Copper is an essential micronutrient that is rigorously regulated for homeostasis, and lipid droplets not only serve as a storage pool for copper but also potentially facilitate its trafficking to other organelles within the cell.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Matthew R. Breuer, Ananya Dasgupta, Joseph G. Vasselli, Xiaorong Lin, Brian D. Shaw, Matthew S. Sachs
Summary: The prevalence and increasing incidence of fungal infections globally is a significant worldwide health problem. Cryptococcosis, primarily caused by the pathogenic yeast Cryptococcus neoformans, is responsible for approximatelyestimated deaths annually. The scarcity of treatments and the increasing resistance to current therapeutics highlight the need for the development of antifungal agents which have novel mechanisms of action and are suitable for clinical use. Repurposing existing FDA-approved compounds as antimycotic therapeutics is a promising strategy for the rapid development of such new treatments. Sertraline (SRT), a commonly prescribed antidepressant, is a broad-spectrum antifungal agent with particular efficacy against C. neoformans. However, the effect of SRT on fungal physiology is not understood. Here, we report that SRT induces the formation of supersized lipid droplets (SLDs) in C. neoformans, and in Candida albicans, Saccharomyces cerevisiae, and Aspergillus fumigatus. SLDs were not induced in C. neoformans by treatment with the antifungal fluconazole (FLC), consistent with SRT and FLC acting differently to perturb C. neoformans physiology. The formation of SLDs in response to SRT indicates that this compound alters the lipid metabolism of C. neoformans. Moreover, the SRT-induced enlargement of LDs in other fungal species may indicate a common fungal response to SRT.
Article
Agricultural Engineering
Ya Zhu Wang, Jin Zheng, Muhammad Nawaz, Fan Yang, Jiajun Hu, Min-Tian Gao
Summary: This study discovered that soluble polysaccharides from rice straw were conjugated with high levels of phenolic compounds. These conjugates significantly affected yeast growth and ethanol fermentation, improving their efficiency. The conjugates reduced osmotic stress and intracellular hydrogen peroxide levels, leading to higher yeast growth and ethanol production.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Munir Ozturk, Mert Metin, Volkan Altay, Luigi De Filippis, Bengu Turkyilmaz Unal, Anum Khursheed, Alvina Gul, Mirza Hasanuzzaman, Kamuran Nahar, Tomonori Kawano, Pedro Garcia Caparros
Summary: Cadmium, a toxic heavy metal from industrial activities, causes various metabolic effects and signaling pathway activations in living beings. Exposure to cadmium stress in Saccharomyces cerevisiae results in protein structural changes, cell cycle disruption, and oxidative stress.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ramy S. Yehia, Ahmed M. Saleh, Mohammad Bani Ismail, Saleh Al-Quraishy, Ohoud Al-Amri, Rewaida Abdel-Gaber
Summary: This study investigated the production, characterization, and bioactivity of yeast mannan. The highest production of mannan was observed in the Saccharomyces cerevisiae isolate RY1. Characterization of RY1 mannan revealed its ability to scavenge radicals and exhibit anti-tumor activity against liver and breast cancer cell lines. The findings confirm the potential of S. cerevisiae mannan as a biotherapeutic agent for cancer treatment.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Cell Biology
Rasha Khaddaj, Muriel Mari, Stephanie Cottier, Fulvio Reggiori, Roger Schneiter
Summary: This study investigates the localization of integral membrane proteins to the surface of lipid droplets (LDs) and confirms that the ER-LD junction acts as a barrier for ER-resident integral membrane proteins. The study also reveals that certain membrane proteins can be targeted to LDs through LD-targeted proteins.
JOURNAL OF CELL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Haotian Xue, Huabin Cao, Chenghong Xing, Jiapei Feng, Linwei Zhang, Caiying Zhang, Guoliang Hu, Fan Yang
Summary: The study demonstrated that selenium has a protective effect against cadmium-induced oxidative stress and autophagy in the brain. Selenium treatment can inhibit cadmium-induced degenerative changes in cells and restore the balance of the redox system. Furthermore, selenium can also suppress cadmium-induced autophagy and activation of the Nrf2 signaling pathway.
Article
Environmental Sciences
Shuqiu Yang, Zhonghua Xiong, Tianfang Xu, Chengcheng Peng, Aiming Hu, Wenjuan Jiang, Zhiwei Xiong, Yunhui Wu, Fan Yang, Huabin Cao
Summary: This study investigated the effects of compound probiotics on intestinal dysfunction and cecal microbiota dysregulation induced by cadmium in broilers. The results showed that cadmium caused intestinal villus damage and inflammation, down-regulated genes related to intestinal barrier and inflammatory factors, and up-regulated certain other genes. However, the addition of compound probiotics effectively improved these changes. Furthermore, the analysis of intestinal microbiota showed that supplementation with compound probiotics increased the abundance of beneficial bacteria and regulated pathways related to metabolism and translation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Chuan Yang, Aoxue Dong, Li Deng, Fang Wang, Junfeng Liu
Summary: Fermentation processes are greatly influenced by thermal changes, particularly in the yield of lipid products; however, the changes in lipid metabolism in response to low temperatures are not well understood. This study utilized transcriptome and lipidomic analysis to investigate alterations in lipid metabolism under low and optimal temperatures. Transcriptome analysis revealed that ribosome biogenesis and carbon metabolism pathways were significantly induced by the drop in temperature. The data also indicated that yeast cells adjust membrane fluidity by modifying the content of different lipid species, rather than the unsaturation level of corresponding fatty acids. The core regulator of energy metabolism in yeast's adaptation to low temperature was identified as Snf1 through transcriptome analysis of glucose-energy metabolism. These findings enhance our understanding of carbon metabolism in Saccha-romyces cerevisiae under cold stress and provide valuable insights for the production of high-value lipid products through metabolic engineering.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Reena R. Varma, Juhee G. Pandya, Jyoti Sharma, Chandramani Pathak, Mohan N. Patel
Summary: In this study, five N, S donor ligands were synthesized and their complexes with rhenium were evaluated for their activity against DNA, cells, and bacteria. The synthesized compounds showed toxicity towards yeast cells and exhibited antiproliferative activity against bacteria and cancer cells.
MOLECULAR DIVERSITY
(2021)
Article
Biotechnology & Applied Microbiology
Yijin Zhao, Yueping Zhang, Jens Nielsen, Zihe Liu
Summary: Regulating lipid metabolic pathways is crucial for increasing beta-carotene accumulation in Saccharomyces cerevisiae. Overexpression of sterol ester synthesis genes and deletion of phosphatidate phosphatase genes significantly enhance beta-carotene yield.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Environmental
Agnieszka Wegrzyn, Avtandil Tsurtsumia, Stefan Witkowski, Olga Freitas, Sonia Figueiredo, Joanna Cybinska, Wojciech Stawinski
Summary: This study found that the addition of vermiculite in yeast can reduce the toxicity of Cd2+ and enhance the adsorption capacity of yeast for Cd2+.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Microbiology
Linru Huang, Zhijia Fang, Jian Gao, Jingwen Wang, Yongbin Li, Lijun Sun, Yaling Wang, Jianmeng Liao, Ravi Gooneratne
Summary: Threonine (Thr) shows strong protective effects against cadmium (Cd) toxicity in yeast cells by preferentially chelating Cd ions, which in turn preserves the activity of superoxide dismutase (SOD) enzyme. This suggests that Thr supplementation could potentially reduce Cd-induced oxidative damage.
JOURNAL OF BASIC MICROBIOLOGY
(2021)
Article
Environmental Sciences
Varsha Dogra, Gurpreet Kaur, Rajeev Kumar, Sandeep Kumar
Summary: In this study, ruthenium and ruthenium oxide nanoparticles derived from different metallosurfactants were synthesized and their nanotoxicity towards Saccharomyces cerevisiae was investigated. Results showed that RuCTAC had the most toxic effect on yeast cells, with RuCTAC and RuHEXA causing the most damage to yeast genomic DNA.
Article
Environmental Sciences
Li Wang, Fan Yang, Mingwen Hu, Guiping Chen, Yun Wang, Haotian Xu, Danghua Fu, He Bai, Guoliang Hu, Huabin Cao
Summary: Cadmium (Cd), a harmful heavy metal, causes nephrotoxicity by accumulating in the kidneys. Selenium (Se) alleviates the toxicity of Cd. This study investigated the protective mechanism of Se against Cd nephrotoxicity, focusing on ferroptosis and pyroptosis. The results showed that Cd impaired kidney function and induced ferroptosis and pyroptosis. However, Se increased the expression levels of protective factors and ameliorated the Cd-induced changes.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Cell Biology
Selvaraj Rajakumar, Nagaraj Bhanupriya, Chidambaram Ravi, Vasanthi Nachiappan
CELL STRESS & CHAPERONES
(2016)
Article
Genetics & Heredity
Chidambaram Ravi, Ramachandran Gowsalya, Vasanthi Nachiappan
Review
Cell Biology
Selvaraj Rajakumar, Albert Abhishek, Govindan Sadasivam Selvam, Vasanthi Nachiappan
CELL STRESS & CHAPERONES
(2020)
Review
Biochemistry & Molecular Biology
Abhishek Albert, Eldho Paul, Selvaraj Rajakumar, Luciano Saso
FREE RADICAL RESEARCH
(2020)
Article
Genetics & Heredity
Selvaraj Rajakumar, Rajendran Vijayakumar, Albert Abhishek, Govindan Sadasivam Selvam, Vasanthi Nachiappan
Article
Biotechnology & Applied Microbiology
Selvaraj Rajakumar, Vennila Suriyagandhi, Vasanthi Nachiappan
FEMS YEAST RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Gowsalya Ramachandran, Ravi Chidambaram, Vasanthi Nachiappan
Summary: The study revealed the role of FSH1 in lipid metabolism. Overexpression of FSH1 led to decreased cellular phospholipid levels, increased triacylglycerol levels, and influenced lipid droplet numbers. The purified recombinant protein Fsh1p was identified as a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS) and impacts lipid homeostasis in S. cerevisiae.
BIOTECHNOLOGY LETTERS
(2021)
Article
Genetics & Heredity
Ramachandran Gowsalya, Chidambaram Ravi, Vasanthi Nachiappan
Summary: This study found that expressing the OVCA2 gene in yeast induces apoptotic cell death, and complementing OVCA2 in fsh3 Delta cells slows cell growth and increases apoptotic phenotypes.
Article
Biochemistry & Molecular Biology
Ravi Chidambaram, Gowsalya Ramachandran, Ram Rajasekharan, Vasanthi Nachiappan
Summary: This study elucidated the important role of the transcription factor GCR1 in lipid metabolism in Saccharomyces cerevisiae. The study confirmed the binding of GCR1 to the OPI3 promoter region and its regulation of OPI3 expression. The study also demonstrated the impact of GCR1 on lipid homeostasis, including the level of phosphatidylcholine and the number and size of lipid droplets.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Rajakumar Selvaraj, Sarah Zehnder, Russell Watts, Jihong Lian, Chinmayee Das, Randal Nelson, Richard Lehner
Summary: Two distinct diacylglycerol acyltransferases (DGAT1 and DGAT2) catalyze the final step of TG synthesis in hepatocytes. TG is either stored in cytosolic LDs or assembled into VLDLs in the ER. DGAT1-made TG is stored in smaller LDs while DGAT2-made TG is stored in larger LDs. ATGL targets DGAT1-generated LDs and the fatty acids released from these LDs are preferentially used for oxidation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Toxicology
Abhishek Raj, Vasanthi Nachiappan
Summary: The study used Saccharomyces cerevisiae as a model organism to investigate the impact of benzene metabolites on cell growth and oxidative stress. Results showed that acute exposure to hydroquinone (HQ) led to increased expression of apoptosis regulatory genes and alterations in phospholipid and neutral lipid levels in wild-type cells.
TOXICOLOGY RESEARCH
(2021)
Article
Toxicology
Abhishek Raj, Vasanthi Nachiappan
TOXICOLOGY RESEARCH
(2019)
Review
Toxicology
Shahid Yousuf Ganie, Darakhshan Javaid, Younis Ahmad Hajam, Mohd Salim Reshi
Summary: Arsenic toxicity is a complex and pervasive public health problem that affects millions of people worldwide. This review aims to consolidate current knowledge on arsenic toxicity, including its sources, chemical forms, and the pathways through which it affects human health. The study found that arsenic is associated with chronic diseases such as cancer, cardiovascular disorders, and neurotoxicity, and oxidative stress, genotoxicity, and epigenetic modifications play important roles in arsenic-induced cellular damage. Further research is needed to fill gaps in our understanding of arsenic toxicity and inform public health policies aimed at minimizing exposure.
TOXICOLOGY RESEARCH
(2024)
Article
Toxicology
Lu Zhang, Min Li, Dalong Zhang, Shujing Zhang, Li Zhang, Xiaojun Wang, Zhiyong Qian
Summary: With the increasing incidence of neurodevelopmental disorders, it is important to screen and evaluate developmental neurotoxicity (DNT) compounds from a large number of environmental chemicals and understand their mechanisms. This study conducted a DNT qualitative structure-activity relationship (QSAR) study for the first time, and preliminarily illustrated the structural characterization of DNT compounds. Multiple models and methods were used to construct prediction models, resulting in the best model with good predictive ability. By combining different models, both MCC values and application domain values were improved. Additionally, through modeling descriptors analysis and structure alerts (SAs) identification, electronical properties, van der Waals volume-related properties, and S, Cl or P containing substructure were found to be associated with DNT. This study lays a foundation for further DNT prediction of environmental exposures in human and contributes to the understanding of DNT mechanism.
TOXICOLOGY RESEARCH
(2024)
Article
Toxicology
Khushboo Sharma, Satish Koundal, Mandeep Singh, Pooja Chadha, Harvinder Singh Saini
Summary: The unregulated discharge of untreated or partially treated industrial wastewater poses a serious threat to aquatic ecosystems. This study exposed Channa punctata fish to untreated and microbially treated textile industry effluent to analyze their toxicity effects. The results showed that the untreated effluent group had more severe hematological, biochemical, histopathological, and ultrastructural alterations compared to the microbially treated group. This highlights the importance of finding more effective microbial treatments to protect aquatic life and human health.
TOXICOLOGY RESEARCH
(2024)
Article
Toxicology
Chen Gu, Yan Sun, Meiqi Mao, Jinguo Liu, Xiongyao Li, Xiaoping Zhang
Summary: This study investigated the mechanism of lunar dust simulant (CLDS-i) in inducing inflammatory lung injury. The results showed that CLDS-i regulated immune inflammatory cells and activated signaling pathways related to inflammatory diseases, promoting the occurrence and development of inflammatory injury. The study also found that amino acid metabolism imbalance might be related to the activation of the NF-kappa B signaling pathway.
TOXICOLOGY RESEARCH
(2024)
