Journal
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Volume 14, Issue 4, Pages 383-391Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
DOI: 10.1007/s13770-017-0060-3
Keywords
Type I collagen; Chondrocyte; Proliferation; Chondrogenic phenotype
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Funding
- National Research Foundation (NRF) - Korea Ministry of Education, Science and Technology, Republic of Korea [2009-0093829]
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It is controversial whether type I collagen itself can maintain and improve chondrogenic phenotype of chondrocytes in a three-dimensional (3D) environment. In this study, we examined the effect of type I collagen concentration in hydrogel (0.5, 1, and 2 mg/ml) on the growth and phenotype expression of rat chondrocytes in vitro. All collagen hydrogels showed substantial contractions during culture, in a concentration-dependent manner, which was due to the cell proliferation. The cell viability was shown to be the highest in 2 mg/ml collagen gel. The mRNA expression of chondrogenic phenotypes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated, particularly in 1 mg/ml collagen gel. Furthermore, the production of type II collagen and glycosaminoglycan (GAG) content was also enhanced. The results suggest that type I collagen hydrogel is not detrimental to, but may be useful for, the chondrocyte culture for cartilage tissue engineering.
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