Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yiqiang Chang, Ansong Ping, Chunyu Chang, Volker M. Betz, Lin Cai, Bin Ren
Summary: This study investigated the effects of lactoferrin (LF) on the osteogenic differentiation of adipose-derived stem cells (ADSCs). The results showed that LF could promote the proliferation and osteogenic differentiation of ADSCs. LF concentration of 100 mu g/mL exhibited the most significant osteoinductive effect. LF could be used as an alternative to bone morphogenetic protein-2 (BMP-2) in bone tissue engineering to promote bone repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Stefano Negri, Yiyun Wang, Takashi Sono, Qizhi Qin, Ginny Ching-Yun Hsu, Masnsen Cherief, Jiajia Xu, Seungyong Lee, Robert J. Tower, Victoria Yu, Abhi Piplani, Carolyn A. Meyers, Kristen Broderick, Min Lee, Aaron W. James
Summary: Research has shown that anti-DKK1 neutralizing antibodies can enhance the osteogenic differentiation of human adipose-derived stem cells, aiding in bone repair, and demonstrating potential beneficial effects in a mouse model.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mei-Ling Ho, Chin-Jung Hsu, Che-Wei Wu, Ling-Hua Chang, Jhen-Wei Chen, Chung-Hwan Chen, Kui-Chou Huang, Je-Ken Chang, Shun-Cheng Wu, Pei-Lin Shao
Summary: This study investigated the effect of adipose-derived stem cell (ADSC)-secreted extracellular vesicles (EVs) on osteoblast function. The results showed that ADSC-EVs can enhance osteoblast proliferation and activity, and may regulate osteoblast function through autophagy or signaling pathways.
Review
Biotechnology & Applied Microbiology
V. K. Anupama Devi, Anjaneyulu Udduttula, Amit Kumar Jaiswal
Summary: Biomedical uses for natural polysaccharides of marine origin, including fucoidan derived from brown algae, are gaining popularity. Despite being less researched compared to other biomaterials, fucoidan is more accessible and cost-effective. This review provides an overview of its history, sources, and characteristics, with a focus on its application in bone tissue engineering. The global research status of fucoidan, especially for bone tissue engineering, is examined, making this study a comprehensive manual for understanding its research status.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Dakshi Kochhar, Megan K. DeBari, Rosalyn D. Abbott
Summary: This review highlights the biophysical properties of silk as a polymeric biomaterial that can dynamically regulate cellular fates, affecting cell behaviors such as adhesion, proliferation, migration, and differentiation. The unique mechanical properties, bioactive component sequestration, degradability, well-defined architectures, and biocompatibility of silk play a crucial role in unlocking the body's inherent regenerative potential.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yu-Hee Kim, Hyun-Ji Lee, Kyung-Ah Cho, So-Youn Woo, Kyung-Ha Ryu
Summary: This study found that conditioned medium obtained from human tonsil-derived MSCs (T-MSC CM) can inhibit adipocyte differentiation by regulating glucocorticoid signal transduction, thereby reducing obesity. The experiments showed that T-MSC CM can attenuate early-stage adipocyte differentiation and protect mice from the negative effects induced by glucocorticoid treatment. Additionally, T-MSC CM enhances megakaryocyte differentiation and protects blood platelet counts.
Article
Biochemistry & Molecular Biology
Piyachat Chuysinuan, Patcharakamon Nooeaid, Thanyaluck Thanyacharoen, Supanna Techasakul, Prasit Pavasant, Kavita Kanjanamekanant
Summary: The study demonstrates the potential use of synthetic HA-incorporated fibroin-alginate hydrogel as a scaffold for bone regeneration in dentistry, showing good biocompatibility. The hydrogel exhibits high swelling ratio and degradation properties in vitro, but further in vivo studies are needed to validate its clinical application potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cell Biology
Tao Zhang, Yuqian He, Xin Shu, Xiaoyu Ma, Jiaqi Wu, Zuoqin Du, Jin Xu, Ni Chen, Jingcan You, Yaofang Liu, Tian Li, Jianbo Wu
Summary: The study found that cultured adipose-derived stem cells (ASCs) undergo cellular senescence during in-vitro passage. It was also discovered that light activation can reduce the expression of senescent genes and SA-beta-Gal in later passage cells. Furthermore, light-activated ASCs-derived exosomes can decrease cellular senescence and SA-beta-Gal expression in later passage cells. Opsin3 (Opn3) in light-activated ASCs was found to be photoreceptive and its deletion affected the effects of light activation on reducing the expression of senescent genes, increasing Ca2+ influx, and cAMP levels. In conclusion, light-activated ASCs can serve as a new protective approach to mitigate cellular senescence during in-vitro passage.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Engineering, Biomedical
R. Tevlin, H. desJardins-Park, J. Huber, S. E. DiIorio, M. T. Longaker, D. C. Wan
Summary: Osteoarthritis is a global burden that affects joints and bones. Tissue engineering, specifically adipose-derived stromal cells, shows promise as a treatment option. Advancements in material science allow for improved engraftment of these cells and activation of biophysical signals through designed microstructures.
Article
Materials Science, Multidisciplinary
Danli Zhu, Wanting Bao, Boxuan Wei, Hao Wei, Jinwu Wang, Guangdong Zhou, Xiansong Wang, Shanyu Guo
Summary: In this study, a strategy for the construction of vascularized tissue-engineered adipose tissue was proposed. By integrating a gas-foamed method (soil) and three-dimensional cell spheroids (seeds), adipose tissue regeneration was achieved. The prepared soil significantly enhanced the vascularization of the regenerated tissue, while optimizing the seed cells promoted adipogenesis. This study provides a solution for the regeneration of vascularized adipose tissue and establishes a general research model for the reconstruction of breast defects.
APPLIED MATERIALS TODAY
(2023)
Article
Biotechnology & Applied Microbiology
Merve G. Kesim, Caner Durucan, Deniz Atila, Dilek Keskin, Aysen Tezcaner
Summary: SIM-loaded and DAT-coated HAp microspheres were developed for bone regeneration. The microspheres demonstrated enhanced drug release and improved cellular response when coated with DAT. Low-dose DAT-coated SIM microspheres hold promise for bone tissue engineering applications.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Lara Milian, Pilar Molina, Maria Oliver-Ferrandiz, Carlos Fernandez-Sellers, Ana Monzo, Rafael Sanchez-Sanchez, Aitana Braza-Boils, Manuel Mata, Esther Zorio
Summary: This study found the feasibility of isolating and characterizing adipose-derived stem cells (ADSCs) from cadaveric samples, and confirmed their ability to differentiate into chondrocytes and osteocytes. This discovery provides potential applications in tissue engineering and research fields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yan Zhang, Cheng Zhang, Jiasheng Wang, Hao Liu, Muyao Wang
Summary: Bone is a metabolically active organ that undergoes constant remodeling throughout life, and adipose tissue disorders strongly influence the development of bone diseases. Adipokines play important roles in the interplay between bone and adipose tissue, while extracellular vesicles have been identified as a novel method of communication between different organs in the field of bone remodeling.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Materials Science, Biomaterials
Masumeh Staji, Negar Sadeghzadeh, Soheila Zamanlui, Mojgan Azarani, Ali Golchin, Masoud Soleimani, Abdolreza Ardeshirylajimi, Arash Khojasteh, Simzar Hosseinzadeh
Summary: This study investigated the synergistic effect of an electrospun scaffold made of polyurethane, gelatin, and human amniotic polymers in the co-culture of mesenchymal stem cells and human keratinocytes. The results showed that this scaffold promoted cell proliferation and epithelial differentiation, and had a significant influence in mice experiments.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Hanieh Nokhbatolfoghahaei, Farshid Bastami, Saeed Farzad-Mohajeri, Maryam Rezai Rad, Mohammad Mehdi Dehghan, Mahboubeh Bohlouli, Hekmat Farajpour, Nasser Nadjmi, Arash Khojasteh
Summary: This study evaluated the use of the masseter muscle as a natural bioreactor for bone regeneration using beta TCP or PCL/beta TCP scaffolds. The results showed that using the masseter muscle as a bioreactor improved bone regeneration. The composition of the scaffold, pedicle preservation, and treatment with MSCs or rhBMP2 also influenced the rate of new bone formation and scaffold degradation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Review
Cell & Tissue Engineering
Hanieh Nokhbatolfoghahaei, Maryam Rezai Rad, Zahrasadat Paknejad, Abdolreza Ardeshirylajimi, Arash Khojasteh
Summary: In this systematic study, the impact of mechanical forces on osteogenesis was reviewed, and it was found that the Wnt signaling pathway was predominantly activated by almost all types of force. All signaling pathways regulate the expression of RUNX2, and in tension force, the mode of force administration is more important than the percentage of elongation.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, General & Internal
Leila Dehghani, Arash Khojasteh, Masoud Soleimani, Saeed Oraee-Yazdani, Saeed Heidari Keshel, Mohammad Saadatnia, Masih Saboori, Alireza Zali, Seyed Mahmoud Hashemi, Reyhane Soleimani
Summary: This study evaluated the safety of intraparenchymal injection of placenta-derived exosomes in patients with malignant middle cerebral artery infarct (mMCAI). The results showed no adverse effects and suggested that local injection of exosomes may be a safe treatment option for patients with acute ischemic stroke and post ischemic disability in the future.
INTERNATIONAL JOURNAL OF PREVENTIVE MEDICINE
(2022)
Article
Polymer Science
Reza Samanipour, Sina Farzaneh, Javad Ranjbari, Sheida Hashemi, Arash Khojasteh, Simzar Hosseinzadeh
Summary: In this study, an oxygen-releasing PCL/SP-PVA scaffold was developed and used as a platform for osteogenic differentiation of dental pulp stem cells. The scaffold showed no inhibitory effect on cell growth and provided a suitable matrix for osteogenic differentiation. The results support the use of oxygen-releasing scaffolds as a potential strategy to accelerate bone regeneration.
Article
Genetics & Heredity
Vahid Reza Yassaee, Arash Khojasteh, Farzad Hashemi-Gorji, Hossein Sadeghi, Hannaneh Safiaghdam, Reza Mirfakhraie
Summary: This study identified a novel pathogenic variant in the ANO5 gene that is responsible for gnathodiaphyseal dysplasia in a large Iranian family.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Materials Science, Textiles
Azadeh Izadyari Aghmiuni, Saeed Heidari Keshel, Mostafa Rezaei-tavirani, Farshid Sefat, Arash Khojasteh, Masoud Soleimani, Farzad Pakdel
Summary: This study explores new approaches to improve skin lesions using tissue engineering. By adjusting the ratios and molecular weights of polymers, and grafting scaffold with silk fibroin biomaterial, a substrate with a skin-like pattern was successfully fabricated. The engineered scaffold exhibited better physicomechanical properties and keratinocyte expression compared to decellularized dermis. This indicates that tissue-engineered scaffolds could be a more suitable option for skin regeneration.
FIBERS AND POLYMERS
(2022)
Article
Engineering, Biomedical
Hannaneh Safiaghdam, Hanieh Nokhbatolfoghahaei, Saeed Farzad-Mohajeri, Mohammad Mehdi Dehghan, Hekmat Farajpour, Hossein Aminianfar, Zeinab Bakhtiari, Massoumeh Jabbari Fakhr, Simzar Hosseinzadeh, Arash Khojasteh
Summary: In this study, the researchers evaluated the effect of adding NanoMgO to PCL/β-TCP scaffolds on bone regeneration. The results suggest that the PCL/β-TCP/nanoMgO scaffold is more suitable as a bone substitute in critical-sized calvarial defects compared to PCL/β-TCP scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Engineering, Biomedical
Mehrdad Seifi, Sadrollah Motamed, Abdolreza Rouientan, Mahboubeh Bohlouli
Summary: There are significant challenges associated with ear cartilage deformities, which pose issues for patients, especially children. However, the development of regenerative medicine offers potential solutions through tissue engineering, cell manipulation, and biomolecule application. The use of both natural and synthetic materials, along with advanced techniques like 3D printing, has shown promise in improving the properties of engineered auricular tissue.
Article
Biochemistry & Molecular Biology
Farshid Bastami, Seyedeh-Mina Safavi, Sina Seifi, Nasser Nadjmi, Arash Khojasteh
Summary: A 3D-printed biodegradable hydrogel has been developed as a scaffold to enhance cell adhesion, proliferation, and osteogenic differentiation. It shows promising potential for bone regeneration, surpassing the conventional clinical approach.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Simzar Hosseinzadeh, Forough Shams, Roya Fattahi, Ghader Nuoroozi, Elnaz Rostami, Lida Shahghasempour, Nasim Salehi-Nik, Mahboubeh Bohlouli, Arash Khojasteh, Nazanin Ghasemi, Habibollah Peiravi
Summary: A hemocompatible substrate enables vascular endothelial cells to produce nitric oxide (NO) in response to inflammation after injuries, inhibiting platelet aggregation in small-diameter vessels. By modifying the surface of polyurethane (PU) films, the concentrations of gelatin-aspirin and gelatin-heparin were optimized for enhanced biocompatibility and bioactivity.
ADVANCED PHARMACEUTICAL BULLETIN
(2023)
Article
Dentistry, Oral Surgery & Medicine
Hadi Assadian, Arash Khojasteh, Zahra Ebrahimian, Fereshteh Ahmadinejad, Helia Sadat Haeri Boroojeni, Mahboubeh Bohlouli, Mohammad Hossein Nekoofar, Paul Mh Dummer, Hanieh Nokhbatolfoghahaei
Summary: This study aimed to compare the response of human dental pulp stem cells (hDPSCs) to three hydraulic calcium silicate cements (HCSCs). The results showed that Biodentine significantly upregulated DMP1 gene expression over 14 days, whereas CEM was only minimally associated with the expression of DSPP and DMP1.
JOURNAL OF APPLIED ORAL SCIENCE
(2022)
Article
Immunology
Hanieh Mojtahedi, Nikoo Hossein-khannazer, Seyed Mahmoud Hashemi, Mina Masoudnia, Monireh Askarzadeh, Arash Khojasteh, Mandana Sattari
Summary: This study evaluated the effects of P. gingivalis and E. coli lipopolysaccharide (LPS) on the gene expression of TLRs and inflammatory cytokines in human dental pulp stem cells. The results showed that both types of LPS stimulate inflammation through increased TLR4 expression, with P. gingivalis LPS showing stronger stimulation within the first 24 hours. However, with prolonged exposure time, both LPS appeared to have anti-inflammatory properties.
IRANIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Emergency Medicine
Mohammad Ali Okhovatpour, Esmaeel Yousefi-Hajivand, Arash Khojasteh, Ali Tabrizi, Alireza Mirahmadi
Summary: Humerus shaft fracture is a common long bone fracture, and the MIPO technique combined with bone graft can achieve better and faster union while reducing tissue dissection and nerve damage.
