Article
Cell Biology
Xiaoyun Wu, Yongxu Mu, Jingyi Yao, Fuhong Lin, Daocheng Wu, Zhijie Ma
Summary: The study revealed that ADSCs from UC patients exhibited defective immunosuppression, indicating their unsuitability for autologous transplantation in treating UC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Tao Wang, Yingyu Li, Yu Zhu, Zebiao Liu, Li Huang, Hongxia Zhao, Zuping Zhou, Qiong Wu
Summary: This study reveals the anti-aging and anti-obesity effects of adipose-derived stem cells (ASCs) in aged animals, while the underlying molecular mechanism remains unknown. The transplantation effects of ASCs from different age donors on natural aging and leptin knockout mice were evaluated, and the multi-omics expression profiles of ASCs from young and aged mouse donors were analyzed. It was found that ASCs from young donors induced weight and abdominal fat loss in older recipients but not in young or leptin knockout mice, and young and aged mouse donor ASCs displayed significant phenotypic differences contributing to the distinguished effects in aged mice.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Biotechnology & Applied Microbiology
Xiaoxue Ren, Xiaoting Gao, Yicheng Cheng, Lingxia Xie, Liping Tong, Wei Li, Paul K. K. Chu, Huaiyu Wang
Summary: In this study, nanoneedle arrays with different heights and widths were fabricated using self-crystallization ability of biocompatible PCL. The substrates were then used to culture BMMSCs and investigate their adhesion, spreading, proliferation ability and multipotency. Different effects were observed on different substrates, with PDA decoration improving adhesion and spreading, but weakening cell-cell interaction and multipotency. The pure PCL nanoneedle arrays, especially PCL1 with a height of 50 nm, were found to maintain the multipotency of BMMSCs through clustered growth with the highest proliferation rate and best multipotential differentiation ability. The study reveals the importance of cell-cell interaction on the multipotency of MSCs and provides a theoretical basis for MSCs behavioral regulation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Samih Mohamed-Ahmed, Mohammed A. Yassin, Ahmad Rashad, Heidi Espedal, Shaza B. Idris, Anna Finne-Wistrand, Kamal Mustafa, Hallvard Vindenes, Inge Fristad
Summary: Comparing the bone regenerative capacity of donor-matched human ASC and BMSC, this study found that BMSC demonstrated higher osteogenic activity and faster bone regeneration rate, making them more suitable for bone regenerative applications.
CELL AND TISSUE RESEARCH
(2021)
Article
Cell & Tissue Engineering
Maria Diniz-da-Costa, Chow-Seng Kong, Katherine J. Fishwick, Thomas Rawlings, Paul J. Brighton, Amelia Hawkes, Joshua Odendaal, Siobhan Quenby, Sascha Ott, Emma S. Lucas, Pavle Vrljicak, Jan J. Brosens
Summary: The study identifies a highly proliferative population of mesenchymal cells (hPMC) in midluteal human endometrium, potentially serving as novel decidual precursors; hPMC differ from endometrial stromal cells in the expression of genes for prolactin and other decidual transcription factors, and show signs of differentiation into a decidual subpopulation discovered in early pregnancy; recurrent pregnancy loss is associated with depletion of hPMC.
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Mohamed Elashry, Mebrie Kinde, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: In this study, it was found that exposure to hypoxia enhanced the proliferation and motility of skeletal muscle-derived stem cells, promoted the differentiation of muscle and adipocytes, and improved the efficiency of myoblast differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, General & Internal
Lucy V. Trevor, Kirsten Riches-Suman, Ajay L. Mahajan, M. Julie Thornton
Summary: This study aims to isolate stromal vascular fraction from human breast tissue exposed to radiotherapy and determine the presence of adipose-derived stem cells. The results show that stromal vascular fraction from irradiated donors has a similar effect in stimulating the migration of dermal fibroblasts as pre-adipocyte conditioned media from healthy donors. Thus, the functionality of adipose-derived stem cells appears to be preserved following radiotherapy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Immunology
Daniel Udenze, Ivan Trus, Sean Lipsit, Scott Napper, Uladzimir Karniychuk
Summary: Congenital virus infections, such as cytomegalovirus and rubella virus infections, commonly affect the central nervous and hematological systems in fetuses and offspring. However, the interactions between congenital Zika virus and the hematological system in fetuses and offspring are largely unknown. This study aimed to determine whether silent in utero Zika virus infection can cause functional and molecular footprints in the bone marrow and blood of fetuses and offspring. The findings showed that in utero Zika virus infection caused multi-organ inflammation in fetuses and local inflammation in the fetal bone marrow, leaving footprints in the offspring's bone marrow and PBMCs.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Biochemistry & Molecular Biology
Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen, Lars Lund, Ditte Caroline Andersen, Soren Paludan Sheikh
Summary: The study found that diabetes does not appear to limit the effectiveness of autologous adipose stem cell therapy for treating erectile dysfunction (ED). Both diabetic and non-diabetic rats' adipose stem cells showed similar abilities in restoring erectile function and inducing genes related to endothelial function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zahra Eydian, Alaleh Mohammad Ghasemi, Samira Ansari, Ali Naghi Kamali, Maryam Khosravi, Sima Momtaz, Sanaz Riki, Laleh Rafighdoost, Reza Entezari Heravi
Summary: This study demonstrates the differentiation of mesenchymal stem cells (MSCs) from human adipose tissue and bone marrow into functional beta-islet cells. It suggests that adipose stem cells may be a better choice for the treatment of diabetes mellitus due to their higher potency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Environmental Sciences
Yifan Pan, Hui Qin, Lu Zheng, Yong Guo, Wei Liu
Summary: HFPO-TA and HFPO-DA have been shown to adversely affect human bone marrow mesenchymal stem cells, with effects on cell cycle processes and promotion of cell proliferation. They also inhibit osteogenic differentiation and increase the expression of core pluripotency regulator NANOG.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Article
Multidisciplinary Sciences
Yu-Hee Kim, Hyun-Ji Lee, Kyung-Ah Cho, So-Youn Woo, Kyung-Ha Ryu
Summary: This study found that conditioned medium obtained from human tonsil-derived MSCs (T-MSC CM) can inhibit adipocyte differentiation by regulating glucocorticoid signal transduction, thereby reducing obesity. The experiments showed that T-MSC CM can attenuate early-stage adipocyte differentiation and protect mice from the negative effects induced by glucocorticoid treatment. Additionally, T-MSC CM enhances megakaryocyte differentiation and protects blood platelet counts.
Article
Engineering, Biomedical
Mattia Vitale, Cosimo Ligorio, Bethan McAvan, Nigel W. Hodson, Chris Allan, Stephen M. Richardson, Judith A. Hoyland, Jordi Bella
Summary: Hydrogels have great potential for tissue engineering applications, but their use in bone regeneration is often hindered by poor mineralization and mechanical properties. Osteoclasts (OCs), which play a pivotal role in bone homeostasis, are often overlooked. This study aims to develop customized, reinforced hydrogels as a platform for studying OC function and differentiation.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Cosimo Ligorio, Aravind Vijayaraghavan, Judith A. Hoyland, Alberto Saiani
Summary: This study investigated the effects of acidic and basic hydrogels on nucleus pulposus cells, with acidic hydrogels accelerating the production of degradative enzymes and pro-inflammatory cytokines.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
C. M. Disney, J. Mo, A. Eckersley, A. J. Bodey, J. A. Hoyland, M. J. Sherratt, A. A. Pitsillides, P. D. Lee, B. K. Bay
Summary: This study used synchrotron computed tomography to investigate the structural and functional differences in collagen fibers within the intervertebral disc. The results showed significant variations in fiber orientation, curvature, and strain between different regions, which are important for understanding tissue compliance and strain transfer.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Alexander Eckersley, Matiss Ozols, Peikai Chen, Vivian Tam, Liam J. Ward, Judith A. Hoyland, Andrew Trafford, Xi -Ming Yuan, Herbert B. Schiller, Danny Chan, Michael J. Sherratt
Summary: Extracellular matrices in the intervertebral disc, lung, and artery undergo age-related damage accumulation, which may influence the progression of age-related diseases. Peptide location fingerprinting analysis identified common structural differences in proteins across multiple organs, species, tissue regions, and genders, which are associated with aging or atherosclerosis.
Review
Biochemistry & Molecular Biology
Matthew J. Kibble, Marco Domingos, Judith A. Hoyland, Stephen M. Richardson
Summary: Back pain, caused by degeneration of the intervertebral discs, is a leading cause of disability worldwide. Understanding the different extracellular matrix cues is crucial for designing effective cellular and regenerative therapies. Omics technologies and modern biofabrication techniques can be used to study matrix molecules and growth factors in disc models, guiding the drivers of healthy cell phenotype and differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Ian P. Smith, Marco Domingos, Stephen M. Richardson, Jordi Bella
Summary: Collagen is commonly used as a biomaterial in regenerative medicine and biomedical research, but its use is limited by sociological and ethical concerns. This study characterizes the properties of collagen derived from jellyfish and compares them with mammalian collagens, providing insights for using alternative collagen sources in biomedical research.
Article
Orthopedics
Shaghayegh Basatvat, Frances C. Bach, Marcos N. Barcellona, Abbie L. Binch, Conor T. Buckley, Brian Bueno, Nadeen O. Chahine, Ana Chee, Laura B. Creemers, Stefan Dudli, Bailey Fearing, Stephen J. Ferguson, Jennifer Gansau, Benjamin Gantenbein, Rahul Gawri, Juliane D. Glaeser, Sibylle Grad, Julien Guerrero, Lisbet Haglund, Paula A. Hernandez, Judith A. Hoyland, Charles Huang, James C. Iatridis, Svenja Illien-Junger, Liufang Jing, Petra Kraus, Lisanne T. Laagland, Gernot Lang, Victor Leung, Zhen Li, Thomas Lufkin, Josette C. van Maanen, Emily E. McDonnell, Chris J. Panebianco, Steven M. Presciutti, Sanjna Rao, Stephen M. Richardson, Sarah Romereim, Tara C. Schmitz, Jordy Schol, Lori Setton, Dmitriy Sheyn, Joseph W. Snuggs, Y. Sun, Xiaohong Tan, Marianna A. Tryfonidou, Nam Vo, Dong Wang, Brandon Williams, Rebecca Williams, S. Tim Yoon, Christine L. Le Maitre
Summary: This study aims to standardize the extraction and expansion techniques for nucleus pulposus (NP) cells, in order to reduce variability between laboratories and improve comparability of results. The most commonly used methods for NP cell extraction, expansion, and re-differentiation were identified through a global survey. Recommended protocols for NP cell culture in different species were provided to support harmonization and cross-lab comparisons.
Article
Biochemistry & Molecular Biology
Xuelian Wang, Daman J. Adlam, Ran Wang, Amal Altujjar, Zhenyu Jia, Jennifer M. Saunders, Judith A. Hoyland, Nischal Rai, Brian R. Saunders
Summary: Injectable hydrogels with moduli close to target tissues are promising for tissue augmentation. Preformed ultra-high molecular weight pH-responsive microgels (DX MGs) interlinked to form hydrogels offer a different approach. By replacing some pH-responsive polymer microgels (MGs) with hydrophilic non-ionic MGs, the mechanical properties of the composite DX MGs can be tuned to achieve gel moduli similar to human nucleus pulposus tissue. These injectable pH-responsive gels show low cytotoxicity and provide a potential system for minimally invasive intervertebral disk augmentation.
Article
Multidisciplinary Sciences
Jack Llewellyn, Venkatesh Mallikarjun, Ellen Appleton, Maria Osipova, Hamish T. J. Gilbert, Stephen M. Richardson, Simon J. Hubbard, Joe Swift
Summary: Cells respond to stress by producing chaperone proteins to maintain protein function, but aging leads to a disruption of protein balance and the formation of disease-related protein aggregates. Understanding the molecular causes of this proteostasis deterioration is important for disease interventions and cell health maintenance in regenerative medicine strategies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Meeting Abstract
Pathology
Michal Dudek, Honour Morris, Yinhui Lu, Karl E. Kadler, Judith A. Hoyland, Qing-Jun Meng
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2023)
Meeting Abstract
Cell & Tissue Engineering
Sam R. Moxon, Marco Domingos, Julie Gough, Stephen M. Richardson
TISSUE ENGINEERING PART A
(2022)
Review
Polymer Science
Cosimo Ligorio, Judith A. Hoyland, Alberto Saiani
Summary: Low back pain caused by intervertebral disc degeneration is a major contributor to global disability. Current treatments are limited, and alternative strategies using soft biomaterials as injectable carriers for disc repair are being explored.
Article
Biotechnology & Applied Microbiology
Alexandra Alcantara Guardado, Alexander Baker, Andrew Weightman, Judith A. Hoyland, Glen Cooper
Summary: Lumbar disc herniation is a common condition that causes lower back pain and sciatica. Microdiscectomy is the most used surgical technique, but it can result in defects in the annulus fibrosus. This paper reviews commercially available products for mechanically closing the annulus and proposes a design specification for a mechanical annular closure strategy.
BIOENGINEERING-BASEL
(2022)
Article
Cell & Tissue Engineering
Dong Wang, Pandi Peng, Michal Dudek, Xueyu Hu, Xiaolong Xu, Qiliang Shang, Di Wang, Haoruo Jia, Han Wang, Bo Gao, Chao Zheng, Jianxin Mao, Chu Gao, Xin He, Pengzhen Cheng, Huanbo Wang, Jianmin Zheng, Judith A. Hoyland, Qing-Jun Meng, Zhuojing Luo, Liu Yang
Summary: This study demonstrates that abnormal mechanical loading can dampen the circadian clock in intervertebral discs (IVDs), leading to intervertebral disc degeneration (IDD). Overexpression of the core clock molecule BMAL1 can attenuate compression-induced IDD. Inhibition of the RhoA/ROCK pathway can restore BMAL1 expression and alleviate IDD. These findings highlight the importance of the circadian clock for IVD homeostasis and provide a potential therapeutic strategy for IDD.