Article
Chemistry, Applied
Liren Xu, Jin Yu, Yunpeng Wang, Yuru Chen, Xiaoli Zhang, Rubing Han, Jing Jing, Rubo Zhang, Xiaoling Zhang
Summary: In this study, a fluorescent probe PCH was synthesized to detect ClO- in mitochondria. PCH can target mitochondria with fast response speed, high selectivity, large Stokes shift, and low detection limitation. With this probe, mitochondrial ClO- can be detected endogenously and exogenously.
Article
Chemistry, Analytical
Xiao-Chuang Chang, Ling Zhang, Bing-Jie Liu, Yan-Tao Lou, Wei-Fan Xu, Xin-Ru Li, Xue-Feng Han, Wei-Na Wu, Yuan Wang, Zhi-Hong Xu
Summary: A novel pyrene-based fluorescent probe was synthesized for the detection of hypochlorite (ClO-) with aggregation-induced ratiometric emission (AIRE) at 525 nm in aqueous solution. The probe showed a fast and sensitive fluorescent response to ClO- within 20 seconds with a detection limit of 269 nM. The sensing mechanism of the probe was investigated using various techniques, and it was successfully applied for imaging endogenous and exogenous ClO- in living cells and zebrafish.
MICROCHEMICAL JOURNAL
(2023)
Article
Spectroscopy
Xiao-Lei Xue, Hao Zhang, Gui-Hua Chen, Guan-Hua Yu, Hao-Ran Hu, Shu-Yan Niu, Kun- Peng Wang, Zhi-Qiang Hu
Summary: There is a pressing need for the development of highly sensitive and selective fluorescence probes for ONOO- in mitochondria. In this study, we introduced a ratiometric fluorescent probe COUS, which utilized a coumarin-cyanine hybrid as the fluorophore and C = C bonds as reaction sites of ONOO-. The probe COUS displayed sensitivity and selectivity towards ONOO-, with a large fluorescence emission shift (239 nm) and a low detection limit (41.88 nM). Furthermore, COUS demonstrated mitochondrial targeting ability, and the targeting moiety could dissociate from the probe upon reaction with ONOO-, enabling accurate detection of ONOO- in mitochondria.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Yu Meng, Chang Liu, Huan Ding, Peiyu Yao, Song He, Xianshun Zeng
Summary: A novel fluorescent probe C-Ptz was synthesized for the detection of ClO- ion. The reaction of C-Ptz with ClO- ions resulted in a fluorescence enhancement (about 42 folds) by blocking PET quenching pathway. The probe C-Ptz showed highly selectivity and fast turn-on response (within 3 s) towards ClO- with detection limit down to 12.6 nM. Furthermore, the fluorescent probe C-Ptz was utilized for fluorescence imaging of ClO- in living cells and measurement of ClO- in real-life water samples.
Article
Chemistry, Analytical
Si-Lin Xu, Fang-Fang Guo, Zhi-Hong Xu, Yuan Wang, Tony D. James
Summary: A water-soluble fluorescent probe 1 based on chromene-hemicyanine has been developed, which exhibits colorimetric fluorescent response towards hypochlorite (ClO-) with a low LOD (0.082 mu M), and turn-on response to viscosity changes. Due to its low cytotoxicity and good biocompatibility, probe 1 is used to monitor hypochlorous acid and viscosity in mitochondria of living cells and zebrafish.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jinshuai Lan, Li Liu, Zhe Li, Ruifeng Zeng, Lixia Chen, Yitian He, Hai Wei, Yue Ding, Tong Zhang
Summary: A novel multi-signal near-infrared fluorescent probe was developed to distinguish biothiols and visualize the metabolism of its derivatives. It showed high sensitivity, selectivity, and rapid fluorescence response, making it a convenient and efficient tool for understanding the physiological functions of biothiols and its metabolism.
Article
Chemistry, Analytical
Keqin Yang, Li Hou, Zhifang Li, Tianran Lin, Jianniao Tian, Shulin Zhao
Summary: A ratiometric fluorescent nanoprobe for detecting peroxynitrite was successfully constructed, showing selective accumulation in mitochondria and linear detection in the range of 0-6.0 µM. This nanoprobe enables ratiometric fluorescence imaging of endogenous peroxynitrite in cell mitochondria.
Article
Spectroscopy
Hai Wu, Qinqin Xu, Kun Yin, Zhaoqiang Liu, Tian Xie, Li Wang, Yuanyuan Li, Mengjie Zhang, Xiaojun Lv, Wenyong Li, Suhua Fan
Summary: A novel fluorescent probe with turn-on fluorescence response, high sensitivity, good compatibility, and mitochondria-targeting function has been synthesized for detecting and visualizing cyanide. The probe utilizes an electron-donating triphenylamine group as the fluorescent, an electron-accepting moiety as the mitochondria-targeted localization unit, and an intramolecular charge transfer system. The probe showed a turn-on fluorescence response towards cyanide due to the insertion of an electron-deficient group and the inhibition of intramolecular charge transfer induced by the nucleophilic addition of cyanide. The probe demonstrated successful detection of cyanide in food samples and showed low cytotoxicity, clear mitochondria-localizing capability, and excellent fluorescence imaging in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Huimin Shangguan, Qianwei Liu, Yuanjuan Wang, Zixuan Teng, Rumeng Tian, Tong Wu, Lei Yang, Ling Jiang, Xingjiang Liu, Liuhe Wei
Summary: In this study, a chromenoquinoline-based ratiometric fluorescent probe was developed for the detection and quantification of hypochlorous acid (HClO). The probe exhibited a large Stokes shift, high synthetic yield, simple synthesis method, short response time, low detection limit, and low toxicity. The probe was successfully validated in live cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Jinshuai Lan, Yu Wang, Yanhong Qin, Zhe Li, Ruifeng Zeng, Li Liu, Lixia Chen, Kaixu Yang, Yue Ding, Tong Zhang
Summary: In this study, a novel dual-site mitochondria-targeted fluorescent probe was developed for the simultaneous detection of SO2 derivatives and ClO-. The probe exhibited distinct fluorescence for each target, and demonstrated good selectivity, high sensitivity and rapid response.
Article
Chemistry, Analytical
Xiaodong Chen, Qian Chen, Dan He, Sanxiu Yang, Yunfei Yang, Jing Qian, Lingliang Long, Kun Wang
Summary: We developed a novel ratiometric NIR fluorescent probe for highly sensitive and selective detection of SO2 derivatives in plant mitochondria. The probe can not only target into the mitochondria but also immobilize in the mitochondria to continue detecting SO2 derivatives even when the mitochondrial membrane potential is collapsed by high concentration of SO2 derivatives. It has been successfully used to monitor high concentration of SO2 derivatives in the plant mitochondria when the plant is suffering from SO2 phytotoxicity, indicating that the interaction of SO2 derivatives with plant mitochondria could be an important mechanism for SO2 phytotoxicity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Multidisciplinary
Keli Zhong, Xiaoling Hu, Shiyi Zhou, Xiuying Liu, Xue Gao, Lijun Tang, Xiaomei Yan
Summary: Hydrogen sulfide (H2S) has benefits for human health and postharvest fruits and vegetables, but can also have negative impacts on some foodstuffs and beverages. A fluorescent turn-on responding probe SPy-DNs has been designed for efficient detection of H2S, exhibiting red emission, large Stokes shift, and nanomolar level detection limit. SPy-DNs shows ultrafast response to H2S, making it a valuable tool for rapid detection in various applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Beidou Feng, Kui Wang, Zhe Wang, Huiyu Niu, Ge Wang, Yuehua Chen, Hua Zhang
Summary: A mitochondrial-targeted probe MBI-OMe was developed to specifically detect changes in ferroptosis-induced ClO-. It showed a ratiometric signal in living cells in response to ferroptosis-induced ClO-.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wei Shu, Jin Yu, Huidan Wang, Anji Yu, Liyan Xiao, Zhuohang Li, Haitao Zhang, Yu Zhang, Yulong Wu
Summary: In this study, a reversible fluorescent probe GeP was designed for detecting intracellular glutathione. The probe exhibited fast response and high fluorescence change, and could target mitochondria. Additionally, GeP enabled superresolution fluorescence imaging of mitochondria.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Lu Sun, Linlin Chen, Zihui Yang, Xuebao Sun, Daojun Jin, Yigui Qiu, Wen Gu
Summary: A novel ratiometric fluorescent probe CPL was designed and synthesized for monitoring HPO42-, which had a significant fluorescent response and demonstrated many advantages. It was successfully applied to detect HPO42- in food samples and for bioimaging.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
