Article
Polymer Science
Safaa Kader, Esmaiel Jabbari
Summary: This study synthesized a sericin-based hydrogel and characterized its properties. The results showed that the gelation time and properties of the hydrogel depended on the degree of sericin modification, and it supported cell viability and growth. Therefore, this sericin hydrogel has the potential to be used for the encapsulation and delivery of stem cells in regenerative medicine.
Article
Biochemistry & Molecular Biology
Shengsheng He, Huijuan Li, Baiyi Chi, Xingjiang Zhang, Yuzhe Wang, Jianxin Wu, Qing Huang
Summary: The main challenge in the field of 3D biomimetic skin is to find a suitable hydrogel matrix that can support the adhesion and proliferation of skin cells. In this study, a photo-crosslinked dual-component hydrogel matrix was constructed using photocurable chondroitin sulfate methacrylate (CSMA) and collagen methacrylate (CoLMA). The hydrogel showed improved mechanical properties and good biocompatibility, making it a promising matrix for constructing 3D biomimetic skin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Zheng, Yan Xie, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated different microenvironmental conditions for the controlled proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The results showed that collagen sponges promoted cell proliferation and chondrogenic differentiation. Combining collagen sponges with collagen hydrogels and induction factors had the highest promotive effect on chondrogenic differentiation. These findings provide useful information for stem cell chondrogenic differentiation and cartilage tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiangjing Yang, Xiaomin Sun, Jia Liu, Yongrui Huang, Yuehai Peng, Yingni Xu, Li Ren
Summary: Corneal transplantation is effective for corneal blindness, but poses risk of bacterial keratitis; the CGL membrane is suitable for corneal repair with proper physicochemical properties and in vitro biocompatibility; addition of GelMA improves cell adhesion force and gene expression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Pablo Sanchez-Cid, Mercedes Jimenez-Rosado, Victor Perez-Puyana, Antonio Guerrero, Alberto Romero
Summary: Tissue engineering research focuses on designing scaffolds with better properties to achieve improved and faster tissue regeneration, thereby enhancing people's quality of life. Collagen, the most abundant protein in animals, is widely used in tissue engineering due to its structure, biocompatibility, ease of modification, and processability, making it an ideal scaffold material.
Article
Engineering, Biomedical
Monize Caiado Decarli, Adrian Seijas-Gamardo, Francis L. C. Morgan, Paul Wieringa, Matthew B. Baker, Jorge Vicente L. Silva, Angela Maria Moraes, Lorenzo Moroni, Carlos Mota
Summary: Cartilage tissue has limited self-repair ability and irreversible progression of lesions. Tissue engineering structures, such as extrusion bioprinted constructs containing stem cell spheroids, show promise for research and therapy. In this study, a reproducible bioprinting process followed by chondrogenic differentiation using hMSC spheroids in a xanthan gum-alginate hydrogel was successfully performed. The bioprinted constructs showed stable structure, cell viability, and matrix production, demonstrating their potential as stable chondral tissue implants.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Zhen Zhang, Yi Liu, Xuelian Tao, Ping Du, Myagmartsend Enkhbat, Khoon S. Lim, Huaiyu Wang, Peng-Yuan Wang
Summary: This study utilized colloidal self-assembled patterns (cSAPs) to prepare cell co-spheroids of human bone mesenchymal stem cells/human umbilical vein endothelial cells (HBMSCs/HUVECs), which were encapsulated in phenol-modified gelatin/hyaluronan (Gel-Ph/HA-Ph) hydrogels and photo-crosslinked using blue light. The Gel-Ph/HA-Ph hydrogels with a 5%-to-0.3% ratio exhibited the best properties. The HBMSC/HUVEC co-spheroids showed enhanced osteogenic differentiation and vascular network formation, and performed better in angiogenesis and blood vessel development in a subcutaneous nude mouse model compared to HBMSC spheroids.
Article
Biotechnology & Applied Microbiology
Wuzheng Xia, Guanzhi Lai, Yichuan Li, Cong Zeng, Chengjun Sun, Pinzhe Zhang, Guanghao Zhu, Leping Li, Linwei Wu
Summary: This study aims to develop a composite hydrogel dressing with excellent hemostatic properties, biocompatibility, and ability to promote liver cell regeneration for the treatment of liver diseases. GelMA/Alg-DA-1 composite hydrogel showed the best physical and chemical properties, good biocompatibility, and hemostatic function. Furthermore, loading adipose-derived mesenchymal stem cell exosomes (AD-MSC-Exo) into GelMA/Alg-DA-1 hydrogel can effectively promote cell proliferation and migration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Marta Michalska-Sionkowska, Oliwia Warzynska, Beata Kaczmarek-Szczepanska, Krzysztof Lukowicz, Anna Maria Osyczka, Maciej Walczak
Summary: Hydrogels based on collagen/beta-glucan crosslinked with tannic acid showed stable properties and improved mechanical performance. They exhibited enhanced antimicrobial and antioxidant properties, positively influencing cell viability.
Article
Materials Science, Paper & Wood
Junyi Chen, Kevin J. Edgar, Charles E. Frazier
Summary: Chemoselective oxidation of oligo(hydroxypropyl)-substituted polysaccharides results in ketones formation at side chain termini, which can readily form hydrogels in situ with amine-containing polymers. These hydrogels exhibit promising properties such as injectability, self-healing, tunable moduli, and responsiveness to various stimuli, making them potential materials for biomedical applications. By introducing methacrylate groups to the oxidized hydroxypropyl cellulose (Ox-HPC) through esterification, UV-induced crosslinking and reinforcement were achieved, significantly enhancing the modulus of the hydrogels. The hydrogels show self-healing and injectability properties both before and after UV curing, demonstrating potential for 3D printing and biomedical applications.
Article
Biochemistry & Molecular Biology
Yin-Cheng Huang, Zhuo-Hao Liu, Chang-Yi Kuo, Jyh-Ping Chen
Summary: A photo-crosslinked hydrogel was developed as a dural substitute and anti-adhesion barrier in this study to address challenges during dural repair, demonstrating biocompatibility, biodegradability, and mechanical strength suitable for use as a dural substitute.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Materials Science, Biomaterials
Matthew Walker, Jiajun Luo, Eonan William Pringle, Marco Cantini
Summary: The extracellular matrix is a complex microenvironment that regulates cell fate, and hydrogels, made of synthetic or natural materials, can mimic these matrices to target cell behavior for tissue engineering. By tuning their properties, native tissue frameworks can be recapitulated. This review explores how hydrogels have been engineered to repair damaged cartilage tissue by harnessing stem cell chondrogenic response, discussing the signaling processes involved in hydrogel-driven chondrogenesis.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Polymer Science
Cristina Manferdini, Diego Trucco, Yasmin Saleh, Elena Gabusi, Paolo Dolzani, Enrico Lenzi, Lorenzo Vannozzi, Leonardo Ricotti, Gina Lisignoli
Summary: The study analyzed the chondrogenic differentiation ability of two different concentrations of VG hydrogels (with RGD motifs) on hASCs. The results showed that VG-RGD 1:2 hydrogels were the most effective in supporting the chondrogenic markers at the gene and protein levels.
Article
Biochemistry & Molecular Biology
Zheng Jing, Yuzhou Li, He Zhang, Tao Chen, Jinrui Yu, Xinxin Xu, Yulong Zou, Xu Wang, Kai Xiang, Xuerui Gong, Ping He, Yiru Fu, Mingxing Ren, Ping Ji, Sheng Yang
Summary: Clinical data shows that smoking is an independent risk factor for osteoporosis by disrupting bone homeostasis. However, little is known about the specific mechanisms of how tobacco toxins affect bone marrow mesenchymal stem cells (BMSCs) and induce osteoporosis. This study found that smokers have significantly lower bone mineral density (BMD) values compared to non-smokers, and exposure to cigarette smoke leads to decreased BMD and dysfunction of rat BMSCs. Further analysis revealed that the ferroptosis pathway is significantly activated in cigarette smoke-exposed BMSCs.
Article
Biotechnology & Applied Microbiology
Jie Xu, Zhenghao Zhang, Xiaofeng Ren, Yunan Zhang, Yang Zhou, Xiaorong Lan, Ling Guo
Summary: Oral mucosal wounds are susceptible to inflammation due to exposure to microorganisms. This study investigated the use of ginsenoside Rg1 in a hydrogel scaffold to treat mucosal defects in rats. The results showed that Rg1-hydrogel exhibited excellent properties and significantly improved wound healing, as evidenced by increased re-epithelialization, collagen deposition, and decreased inflammation. Molecular biology studies confirmed that Rg1-hydrogel stimulated repair-related factors and inhibited inflammatory factors secretion. These findings suggest that Rg1-hydrogel holds promise for the therapeutic management of oral mucosal wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Lu Yuan, Bao Li, Jirong Yang, Yilu Ni, Yingying Teng, Likun Guo, Hongsong Fan, Yujiang Fan, Xingdong Zhang
TISSUE ENGINEERING PART A
(2016)
Article
Biochemistry & Molecular Biology
Yongli Gao, Bao Li, Weili Kong, Lu Yuan, Likun Guo, Chuan Li, Hongsong Fan, Yujiang Fan, Xingdong Zhang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Microbiology
Lu Yuan, Henny C. van der Mei, Henk J. Busscher, Brandon W. Peterson
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Nanoscience & Nanotechnology
Lu Yuan, Hao Wei, Xiao-Yu Yang, Wei Geng, Brandon W. Peterson, Henny C. van der Mei, Henk J. Busscher
Summary: The strength of interaction in encapsulating shells affects the protection of probiotic bacteria, with alginate-hydrogel-based shells showing the best protection against simulated gastric acid and antibiotics. Strong interaction upon ZIF-8 encapsulation causes cell wall damage and slightly reduces bacterial viability, delaying growth. Synergy between alginate-hydrogel-encapsulated B. breve and antibiotics is promising for treating antibiotic-resistant E. coli infections in the gastrointestinal tract.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Bo Zhang, Yumei Qin, Li Yang, Ye Wu, Nuoya Chen, Mingyu Li, Yanyan Li, Huining Wan, Daihua Fu, Rifang Luo, Lu Yuan, Yunbing Wang
Summary: Localized drug delivery from drug-eluting stents can provide therapeutic efficacy with minimal systemic toxicity. The researchers in this study developed a micro- to nanoscale network assembly composed of copper ion and epigallocatechin gallate (EGCG), which can release rapamycin and promote endothelial cell growth. The coated stents exhibited long-term antithrombotic efficacy and attenuated the negative impact of rapamycin on endothelial cells.
Article
Engineering, Biomedical
Bo Zhang, Yumei Qin, Li Yang, Huining Wan, Lu Yuan, Yunbing Wang
Summary: This study developed a new stent that combines the biological functions of nitric oxide gas and VEGF to promote the proliferation and migration of endothelial cells while suppressing smooth muscle cells. The functionalized stent showed enhanced vascular healing and reduced inflammation and vessel wall injury.
Article
Microbiology
Yue Wang, Jingchun Kong, Xiaodong Zhang, Yan Liu, Zeyu Huang, Lu Yuan, Ying Zhang, Jianming Cao, Lijiang Chen, Yong Liu, Tieli Zhou
Summary: The emergence and spread of multi-drug resistant strains globally have raised concerns, especially with the re-emergence of colistin-resistant bacteria. A study has found that the combination of plumbagin and colistin effectively inhibits colistin-resistant P. aeruginosa, showing promising results in both in vitro and in vivo settings. This combination not only prevents biofilm formation but also eradicates mature biofilms, providing a potential treatment option for COL-R PA infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yaran Wang, Jianan Zhou, Lu Yuan, Fan Wu, Lingping Xie, Xiaojian Yan, Huaping Li, Yuanfeng Li, Linqi Shi, Rongdang Hu, Yong Liu
Summary: This study reports the development of carboxylic acid-containing nano-networks with enzymatic activity for combating bacterial biofilms. The optimal nano-networks demonstrated high catalytic efficiency in damaging cell membranes and extracellular DNA of Streptococcus mutans biofilms. The neighboring carboxyl group aids the adsorption, free radical generation, and catalyst reactivation, resulting in superb catalytic efficiency. The nano-networks show great potential in inhibiting tooth decay and treating biofilm-associated diseases without affecting the commensal oral flora.
Article
Chemistry, Analytical
Lu Yuan, Herve Straub, Liubov Shishaeva, Qun Ren
Summary: This article summarizes the application of microfluidics in biofilm research, including understanding the mechanism of bacterial adhesion and biofilm development, assessment of antifouling and antimicrobial properties, development of advanced in vitro infection models, and advancement in methods to characterize biofilms.
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Lu Yuan, Pim de Haan, Brandon W. Peterson, Ed D. de Jong, Elisabeth Verpoorte, Henny C. van der Mei, Henk J. Busscher
MICROSCOPY AND MICROANALYSIS
(2020)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
