Article
Endocrinology & Metabolism
Qiuxia Han, Xiaochen Wang, Xiaonan Ding, Jing Hao, Qi Li, Jifeng Wang, Hanjie Yu, Zhen Tang, Fuquan Yang, Guangyan Cai, Dong Zhang, Hanyu Zhu
Summary: Salivary glycopatterns could be used to differentiate between diabetic nephropathy (DN) and non-diabetic renal disease (NDRD). Levels of AAL, LEL, LCA, VVA, and NPA were correlated with DN severity, while levels of LEL and LCA could indicate the prognosis of DN.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Lina Liu, Dan Li, Jian Shu, Li Wang, Fan Zhang, Chen Zhang, Hanjie Yu, Mingwei Chen, Zheng Li, Xuan Guo
Summary: By investigating the alterations of protein glycopatterns in BALF, this study provided new possibilities for the diagnosis of lung cancer, showing potential value in the identification and early diagnosis of lung cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Jiayi Yang, Shiyu Jin, Simeng Zhang, Shujun He, Rui Chen, Yinghu Lei, Ruliang Pan, He Zhang, Zhongfu Wang, Zheng Li, Hanjie Yu, Guixin Dong, Changhong Shi, Baoguo Li, Songtao Guo
Summary: The study shows that the urinary glycome could serve as promising biomarkers for detecting pregnancy diagnosis and sex in animals. Lectin microarray combining mass spectrometry is effective in distinguishing individuals' pregnancy status and sex, but the glycopatterns appear to be species-specific.
Review
Medicine, General & Internal
Goren Saenz-Pipaon, Saioa Echeverria, Josune Orbe, Carmen Roncal
Summary: Diabetic kidney disease is the leading cause of end stage renal disease in developed countries, with a multifactorial pathogenesis leading to clinical manifestations such as proteinuria, hypertension, and gradual decline in kidney function. Traditional treatments can slow down DKD progression but cannot completely halt it, emphasizing the urgent need for novel early diagnosis and therapeutic targets.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell & Tissue Engineering
Lei Peng, Yu Chen, Shaoqing Shi, Heling Wen
Summary: Diabetic nephropathy remains the most common cause of end-stage renal disease, and early diagnosis and treatment can slow down its progression. Circulating levels of kidney-released exosomes and biomolecules have shown potential in predicting the occurrence and progression of diabetic nephropathy. Stem cell therapy and medications targeting inflammatory, oxidant, or pro-fibrotic pathways are being investigated as potential treatments.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemical Research Methods
Tatchanun Ngamdee, Thanit Chalermwatanachai, Chonpiti Siriwan, Oranut Warachit, Patsamon Rijiravanich, Werasak Surareungchai
Summary: In this study, a nucleic acid amplification-free miRNA-124 sensor was developed for the rapid detection and quantification of T2DN. Using magnetic beads-based target-induced strand displacement and methylene blue-labeled silica particles, the sensor allows measurement through visual observation, spectrophotometry, or electrochemistry. The assay demonstrated low detection limits and a short total analysis time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiameng Ren, Jian Shu, Junhong Wang, Yonghong Guo, Ying Zhang, Lixin Yue, Hanjie Yu, Wentian Chen, Chen Zhang, Jiancang Ma, Zheng Li
Summary: This study aimed to explore the glycosylation of salivary proteins associated with papillary thyroid cancer (PTC) and assess their potential as biomarkers. SVM was found to have the best diagnostic effect based on the changes of salivary glycopatterns. Salivary glycopatterns may also serve as potential biomarkers for prognosis of PTC patients.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Medicine, Research & Experimental
Ye Feng, Xin Zhong, Hai-Feng Ni, Cui Wang, Tao-Tao Tang, Li-Ting Wang, Kai-Yun Song, Ri-Ning Tang, Hong Liu, Bi-Cheng Liu, Lin-Li Lv
Summary: The study revealed that urinary small EVs derived CCL21 mRNA may serve as an early biomarker for identifying DN linked with pathogenesis, and CCL21 mRNA-mediated T cell infiltration may constitute the key mechanism of chronic inflammation in DN.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Endocrinology & Metabolism
Hamad Ali, Mohamed Abu-Farha, Maha M. Hammad, Sriraman Devarajan, Yousif Bahbahani, Irina Al-Khairi, Preethi Cherian, Zahra Alsairafi, Vidya Vijayan, Fahd Al-Mulla, Abdulnabi Al Attar, Jehad Abubaker
Summary: The study identifies N-cadherin as a novel marker for diabetic nephropathy, showing significantly higher levels in diabetic patients with DN compared to those without DN. The increase in N-cadherin occurs early during disease development and correlates with renal markers.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Oke-Oghene Philomena Akpoveso, Emeka Emmanuel Ubah, Gideon Obasanmi
Summary: The global prevalence of diabetes is increasing due to urbanization and an aging population, leading to an increase in diabetic vascular complications. There is a need for safe and effective alternative therapies, with natural products like polyphenols showing potential. Polyphenols can reduce oxidative stress and hyperglycemia, preventing diabetic complications. This review summarizes the effects of polyphenols on endogenous antioxidant systems and discusses therapeutic strategies for diabetic vascular complications.
Article
Biotechnology & Applied Microbiology
Chunrong Huang, Lian-Fang Xue, Bo Hu, Huan-Huan Liu, Si-Bo Huang, Suliman Khan, Yu Meng
Summary: In this study, calycosin-loaded nanoliposomes were successfully fabricated and characterized, showing slow-release properties and high stability. Pharmacokinetic assays demonstrated improved bioavailability of calycosin in nanoliposome form compared to free calycosin, suggesting potential benefits in treating diabetic nephropathy.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Endocrinology & Metabolism
Ming Yang, Chongbin Liu, Na Jiang, Yan Liu, Shilu Luo, Chenrui Li, Hao Zhao, Yachun Han, Wei Chen, Li Li, Li Xiao, Lin Sun
Summary: The endoplasmic reticulum (ER) is an active organelle involved in various physiological processes. Recent studies have shown that dysfunction of the ER plays a role in the progression of kidney disease, particularly diabetic nephropathy (DN). This review discusses the function of the ER, the regulation of homeostasis through the unfolded protein response (UPR) and ER-phagy, as well as the impact of abnormal ER homeostasis in renal cells in DN. Additionally, potential therapeutic targets for DN related to maintaining ER homeostasis are also explored.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Om Prakash Dwivedi, Karina Barreiro, Annemari Karajamaki, Erkka Valo, Anil K. Giri, Rashmi B. Prasad, Rishi Das Roy, Lena M. Thorn, Antti Rannikko, Harry Holthoefer, Kim M. Gooding, Steven Sourbron, Denis Delic, Maria F. Gomez, Per-Henrik Groop, Tiinamaija Tuomi, Carol Forsblom, Leif Groop, Maija Puhka
Summary: By genome-wide sequencing of 200 urinary extracellular vesicle (uEV) mRNA samples from clinical studies, we identified potential candidate biomarkers and mechanisms for studying diabetic kidney disease (DKD) in Type 1 diabetes (T1D), which were validated in both Type 1 and 2 diabetes. The sequencing results revealed over 10,000 mRNAs with similarity to the kidney transcriptome. Thirteen upregulated genes predominantly expressed in proximal tubules, correlated with hyperglycemia and involved in cellular/oxidative stress homeostasis were found in T1D DKD groups. We constructed a transcriptional stress score based on six of these genes (GPX3, NOX4, MSRB, MSRA, HRSP12, and CRYAB), which reflected long-term decline of kidney function and could identify individuals with early decline even in normoalbuminuric patients.
Review
Cell Biology
Hong Zhou, Wei-Jian Ni, Xiao-Ming Meng, Li-Qin Tang
Summary: miRNAs play pivotal roles in the inflammatory and immune processes of DN, affecting the progression of the disease by modulating various aspects of immune and inflammatory responses. Understanding the mechanisms and signaling pathways of miRNAs can lead to the identification of new therapeutic targets and strategies for the treatment of DN.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Jiaqiong Lin, Xiaoyong Li, Yan Lin, Zena Huang, Fei He, Fu Xiong
Summary: In this study, FOS was identified as a promising diagnostic biomarker for diabetic nephropathy, and emetine was discovered as a potential therapeutic candidate. These findings contribute to a better understanding of the pathogenesis of diabetic nephropathy and offer new prospects for treatment strategies.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Review
Biochemical Research Methods
Hanjie Yu, Jian Shu, Zheng Li
EXPERT REVIEW OF PROTEOMICS
(2020)
Article
Oncology
Lina Liu, Dan Li, Jian Shu, Li Wang, Fan Zhang, Chen Zhang, Hanjie Yu, Mingwei Chen, Zheng Li, Xuan Guo
Summary: By investigating the alterations of protein glycopatterns in BALF, this study provided new possibilities for the diagnosis of lung cancer, showing potential value in the identification and early diagnosis of lung cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jian Shu, Jun Ma, Xiameng Ren, Jian Wang, Yan Wang, Kun Zhang, Hanjie Yu, Xiangqian Guo, Zheng Li
Summary: This study investigated the altered salivary glycopatterns between ESCC patients and healthy volunteers, and found significant differences in the glycopatterns of ESCC patients' saliva. The study suggests that GlcNAc or Gal β 1-4GlcNAc-containing N-glycans recognized by lectins like DSA may serve as potential biomarkers for the diagnosis of ESCC.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Wentian Chen, Yaogang Zhong, Jian Shu, Hanjie Yu, Zhuo Chen, Xiameng Ren, Ziye Hui, Zheng Li
Summary: This study isolated and identified GlcBPs from 30 healthy volunteers and 30 T2DM patients using GMPC. Further analysis revealed the main gene annotations and pathways of GlcBPs, as well as potential glucose-binding domains. A short peptide identified through Motif-X analysis showed high-binding ability to glucose.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Xilong Wang, Lixin Yue, Liuyi Dang, Jiajun Yang, Zhuo Chen, Xiurong Wang, Jian Shu, Zheng Li
Summary: This study investigated the role of sialylated glycans on bovine lactoferrin (bLf) against influenza A virus (IAV). The results showed that destroying the sialylated glycans on bLf reduced its binding activity to IAV and its ability to inhibit IAV attachment to host cells, indicating the importance of sialylated glycans in blocking IAV attachment during early stages of viral infection.
GLYCOCONJUGATE JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jian Shu, Hanjie Yu, Xiameng Ren, Yan Wang, Kun Zhang, Zhen Tang, Liuyi Dang, Wentian Chen, Baozhen Li, Hailong Xie, Zheng Li
Summary: The microbiota in the oral cavity plays a crucial role in maintaining human health. This study found that alterations in salivary glycopatterns in gastric cancer patients might contribute to dysbiosis of the oral microbiota. Additionally, specific salivary glycopatterns were shown to affect the adhesion, toxicity, glycan structures, and immune responses of Aggregatibacter segnis. These findings have important implications for developing carbohydrate drugs to maintain a balanced oral microbiota.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Xiameng Ren, Jian Shu, Junhong Wang, Yonghong Guo, Ying Zhang, Lixin Yue, Hanjie Yu, Wentian Chen, Chen Zhang, Jiancang Ma, Zheng Li
Summary: This study aimed to explore the glycosylation of salivary proteins associated with papillary thyroid cancer (PTC) and assess their potential as biomarkers. SVM was found to have the best diagnostic effect based on the changes of salivary glycopatterns. Salivary glycopatterns may also serve as potential biomarkers for prognosis of PTC patients.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Medical Laboratory Technology
Zhen Tang, Fan Zhang, Yuan Wang, Chen Zhang, Xia Li, Mengqi Yin, Jian Shu, Hanjie Yu, Xiawei Liu, Yonghong Guo, Zheng Li
Summary: This study demonstrated alterations in salivary glycopatterns in patients with HC and HCC, with significant differences in glycopatterns recognized by 22 lectins in the saliva of HC and HCC patients and healthy volunteers. The Random Forest algorithm showed the best predictive performance in constructing models for HC and HCC, with AUCs of 0.857 and 0.886 in the validation cohort, respectively.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Microbiology
Jing Dang, Jian Shu, Ruiying Wang, Hanjie Yu, Zhuo Chen, Wenbo Yan, Bingxiang Zhao, Li Ding, Yuzi Wang, Huizheng Hu, Zheng Li
Summary: This study analyzed the differences in glycan alterations between drug-sensitive and carbapenem-resistant Pseudomonas aeruginosa strains using lectin microarrays. The researchers constructed models using the gradient boosting decision tree algorithm to differentiate bacterial resistance based on the screened glycopatterns. The results showed significant differences in glycan structures on the bacterial surface and identified potential biomarkers for bacterial resistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Rheumatology
Hanjie Yu, Mingxiu Li, Jian Shu, Liuyi Dang, Xin Wu, Yuzi Wang, Xuan Wang, Xin Chang, Xiaojuan Bao, Bojing Zhu, Xiameng Ren, Wentian Chen, Yi Li
Summary: This study revealed abnormal glycopatterns and heterogeneity of site-specific glycosylation associated with osteoarthritis (OA). It is the first time that the heterogeneity of site-specific N-glycans was reported in OA cartilage. Gene expression analysis suggested that glycosyltransferases expression was impacted by pro-inflammatory cytokines, which may accelerate protein degradation and the progression of OA. These findings provide valuable information for understanding the molecular mechanisms in the pathogenesis of OA.
ARTHRITIS RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Jian Shu, Xiameng Ren, Hongwei Cheng, Shiyi Wang, Lixin Yue, Xia Li, Mengqi Yin, Xiangqin Chen, Tiantian Zhang, Ziye Hui, Xiaojuan Bao, Wanghua Song, Hanjie Yu, Liuyi Dang, Chen Zhang, Jun Wang, Qi Zhao, Zheng Li
Summary: This study reveals the impact of benign lesions on salivary glycopatterns and cancer screening, and demonstrates that adding benign diseases to the control group improves the performance of the classifier.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Rheumatology
Hanjie Yu, Mingxiu Li, Xiaodong Wen, Jie Yang, Xiaojun Liang, Xia Li, Xiaojuan Bao, Jian Shu, Xiameng Ren, Wentian Chen, Zheng Li, Yi Li
Summary: Elevated α-1,3 fucosylation was observed in OA patients and rabbit models, and was associated with inflammation. Silencing FUT10 gene expression weakened the binding ability of TNFR1 to TNF-α, inhibited activation of NF-kappa B and P38/JNK-MAPK pathways, and ultimately slowed down ECM degradation, senescence, and apoptosis in chondrocytes exposed to TNF-α.
ARTHRITIS RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Hui Zi-Ye, Yu Han-Jie, Shu Jian, Ren Xia-Meng, Chen Wen-Tian
Summary: This review summarizes the latest studies on glycosylation in coronaviridae members, including the crucial role of viral glycosylation in host recognition, immunity avoidance, virus replication, and transmission. It focuses on the distribution and structure of glycosylation in the spike protein. The glycan pattern of recombinant viral glycoproteins may differ from that of native viral proteins, which is important for vaccine design. Recent advances in bioinformatics, biochip, mass spectrography, and genetic technology provide a comprehensive perspective for glycosylation research in coronaviruses.
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Liyuan Jia, Tianran Ma, Yiqian Liang, Haoqi Du, Jian Shu, Xiawei Liu, Zhiwei Zhang, Hanjie Yu, Mingwei Chen, Zheng Li
