Review
Oncology
Hamid Bakherad, Fahimeh Ghasemi, Maryam Hosseindokht, Hamed Zare
Summary: Breast cancer is a common and deadly disease in women, especially triple-negative breast cancer. Nanobodies, with their unique advantages, have the potential to be used in the diagnosis and treatment of breast cancer. However, there is a lack of molecular diagnostic methods specifically for triple-negative breast cancer. Therefore, further research is needed to develop nanobodies against other antigens expressed in triple-negative breast cancer.
CANCER CELL INTERNATIONAL
(2022)
Review
Oncology
Maryam Hosseindokht, Hamid Bakherad, Hamed Zare
Summary: The research explores the application of nanobodies in targeting and sensing prostate cancer cells, aiming at efficient identification and elimination of cancer cells. These specialized nanobodies derived from camel heavy chain antibodies show promising potential in biosensors and cancer diagnostic kits.
CANCER CELL INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Huaping Tang, Yuan Gao, Jiangyuan Han
Summary: The camelid-derived single chain antibody (sdAb), or nanobody, is a unique functional heavy-chain antibody that has high solubility and similar antigen-binding affinity to conventional antibodies, making it a promising tool in biomedicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Chaolemeng Bao, Quanli Gao, Lin-Lin Li, Lu Han, Bingxiang Zhang, Yijin Ding, Zongpei Song, Ruining Zhang, Jishuai Zhang, Xian-Hui Wu
Summary: The use of nanobodies as the antigen binding domain in CAR-T therapy has shown efficacy in targeting various tumor-specific antigens both in vitro and in vivo. Nanobody-modified CAR-T demonstrates comparable clinical effects to traditional CAR-T and presents great potential for addressing more challenging tasks.
Article
Biochemistry & Molecular Biology
Eric Moeglin, Lina Barret, Bruno Chatton, Mariel Donzeau
Summary: This study describes a method for site-specific labeling of nanobodies using two co-assembling peptide tags, E3 and K3. The method provides a simple and robust approach for associating VHH and fluorescent probes, and demonstrates the potential application of these molecular probes in cell immuno-staining.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Zihan Yin, Qiyi He, Huiyi Yang, Christophe Morisseau, El-Sayed A. El-Sheikh, Dongyang Li, Bruce D. Hammock
Summary: A simple, sensitive, and low-cost sandwich ELISA method was developed to quantify heavy single-chain antibodies (VHHs). The method had a sensitivity of 0.149 OD·mL/ng and a limit of detection of 0.029 ng/mL. The method was applied to detect high levels of VHHs in bacterial cell media, raising attention to protein losses in cell culture supernatants and providing a continuous detection method for optimizing expression and purification protocols for nanobodies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Yan Yan, Xiao Cheng, Lin Li, Rumeng Zhang, Yong Zhu, Zhengsheng Wu, Keshuo Ding
Summary: The study successfully constructed HER2-nanobody and demonstrated its ability to inhibit growth and promote apoptosis of HER2-positive breast cancer cells. HER2-nanobody exerts its tumor suppressive role by inhibiting the phosphorylation of ERK and AKT.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Ines Zettl, Tatiana Ivanova, Mohammed Zghaebi, Marina Rutovskaya, Isabella Ellinger, Oksana Goryainova, Jessica Kollarova, Sergio Villazala-Merino, Christian Lupinek, Christina Weichwald, Anja Drescher, Julia Eckl-Dorna, Sergei Tillib, Sabine Flicker
Summary: This study reported the generation and characterization of ICAM-1-specific nanobodies, which showed high affinity binding to ICAM-1 and were not internalized by cells. The findings suggest that these nanobodies can be used in combination with allergen-specific nanobodies to develop topical treatments for pollen allergy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Serena D'Agostino, Elisa Mazzega, Katja Pracek, Sara Piccinin, Flavia Pivetta, Michela Armellin, Sara Fortuna, Roberta Maestro, Ario de Marco
Summary: The study showed that interfering with the p53:Twist1 complex could alleviate Twist1's inhibitory effect on p53, providing potential therapeutic tools for p53 wild type tumors. Using VHH nanobodies targeting the p53 C-terminal region, the research demonstrated the possibility of this intervention and validated its activity in human cell models. These findings provide insights into p53 biology and the development of targeted therapeutic approaches.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Shahryar Khoshtinat Nikkhoi, Geng Li, Suha Eleya, Ge Yang, Venu Gopal Vandavasi, Arash Hatefi
Summary: The objective of this study was to develop a bispecific killer cell engager (BiKE) with high affinity and selectivity for CD16a receptor to improve NK cell-based cancer immunotherapy. High affinity anti-CD16a and anti-HER2 VHH clones were isolated using phage display and fused to create BiKE:HER2/CD16a. The results showed that BiKE:HER2/CD16a can activate NK cells to release cytokines and kill HER2(+) cancer cells more effectively than existing monoclonal antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Julia Hambach, Anna Marei Mann, Peter Bannas, Friedrich Koch-Nolte
Summary: This article discusses the specificity and effectiveness of nanobody-based constructs in targeting CD38-expressing myeloma cells. Promising results have been obtained in preclinical studies, warranting further clinical research to evaluate the potential of these CD38-specific nanobody-based constructs for the treatment of multiple myeloma.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Jan Gettemans, Brian De Dobbelaer
Summary: Single-domain antibodies derived from camelids and sharks, known as nanobodies, have gained attention for their high specificity and affinity binding to targets. Their key features including single-domain nature, small size, affordable high-level expression, and flexibility in altering primary structure have made them appealing for researchers and valuable in biomedical research.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Medicine, Research & Experimental
Mahmoud Ganji, Pooria Safarzadeh Kozani, Fatemeh Rahbarizadeh
Summary: Monoclonal antibody-based immunotherapies have shown promising results in treating immunological and oncological conditions. CD19 is a qualified antigen for B-cell neoplasms treatment, and VHHs have advantages over conventional mAbs as potential therapeutics and diagnostic tools. By isolating CD19-specific VHHs from a novel immune library using phage display, it was demonstrated that selected VHHs exhibited high specificity, sensitivity, and affinity to CD19, making them potential targeting tools for CD19-based immunotherapeutics.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Allergy
Ines Zettl, Tatiana Ivanova, Maria R. Strobl, Christina Weichwald, Oksana Goryainova, Evgenia Khan, Marina Rutovskaya, Margarete Focke-Tejkl, Anja Drescher, Barbara Bohle, Sabine Flicker, Sergei Tillib
Summary: The study identified high-affinity Bet v 1-specific nanobodies that can recognize an important IgE epitope and reduce allergen-induced basophil activation, indicating that allergen-specific nanobodies are useful tools for future treatment of pollen allergy.
Article
Green & Sustainable Science & Technology
Hoda Nouri, Mehrdad Azin, S. Latif Mousavi
ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY
(2018)
Article
Infectious Diseases
Sajad Abdollahi, Iraj Rasooli, Seyed Latif Mousavi Gargari
INFECTION GENETICS AND EVOLUTION
(2018)
Article
Immunology
Sajad Abdollahi, Iraj Rasooli, Seyed Latif Mousavi Gargari
MICROBIAL PATHOGENESIS
(2018)
Article
Biochemistry & Molecular Biology
Mahmood Nazari, Seyed Latif Mousavi Gargari, Abbas Sahebghadam Lotfi, Mohammad Javad Rassaee, Ramezan Ali Taher
Article
Immunology
Zahra Aghajani, Iraj Rasooli, Seyed Latif Mousavi Gargari
Article
Biochemistry & Molecular Biology
Mahya Ranjbar, Bahador Behrouz, Fatemeh Norouzi, Seyed Latif Mousavi Gargari
MOLECULAR IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Hossein Aghamollaei, Mostafa Ghanei, Mohammad Javad Rasaee, Ali Mohammad Latifi, Hamid Bakherad, Mahdi Fasihi-Ramandi, Ramezan Ali Taheri, Seyed Latif Mousavi Gargari
Summary: A novel recombinant nanobody named V80 was isolated and produced using phage display technique, showing high specificity and affinity to cancer cells, making it a potential new tool for cancer detection and therapy.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2021)
Article
Immunology
Fatemeh Norouzi, Bahador Behrouz, Mahya Ranjbar, Seyed Latif Mousavi Gargari
JOURNAL OF IMMUNOLOGY RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Abolfazl Hajialibeigi, Jafar Amani, Seyed Latif Mousavi Gargari
Summary: In this study, new vaccine candidates against Shigella flexneri were identified and experimentally evaluated through reverse vaccinology approach. The results demonstrate the potential of the identified antigens for vaccine development against shigellosis and support the use of reverse vaccinology for discovering novel effective antigens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Homa Ahmadi Rouzbahani, Seyed Latif Mousavi Gargari, Shahram Nazarian, Sajad Abdollahi
Summary: The study developed an oral vaccine based on lactic acid bacteria against ETEC, a leading cause of diarrhea in children. Experimental results showed that oral administration of the engineered L. lactis significantly increased the production of IgA in the intestine, neutralized the toxin, and reduced the pathological symptoms caused by diarrhea.
CURRENT MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mohammad Fatemi Motlagh, S. Latif Mousavi Gargari
Summary: This study aimed to design a recombinant vaccine NeCoVac to improve protection against necrotic enteritis and coccidiosis. The results showed that immunization with NeCoVac significantly protected birds from both diseases, representing a novel approach in vaccine development against these two poultry diseases.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Atefeh Pourdadashi, Razieh Rezaei Adriani, Seyed Latif Mousavi Gargari
Summary: The research isolated single-stranded DNA aptamers with high affinity for different bacterial genera using the SELEX method. Aptamers C65 and C50 showed the highest affinity towards targets in 20 rounds of cell-SELEX. The selected aptamers successfully detected clinical bacterial strains from meningitis patients, demonstrating the potential for developing diagnostic tools.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Zahra Sadat Moshref, Tahmineh Jalali, Razieh Rezaei Adriani, Elahe Soltati, Seyed Latif Mousavi Gargari
Summary: The emergence of the SARS-CoV-2 virus has led to severe acute respiratory syndrome with high mortality worldwide. Early and accurate detection of the virus is crucial in controlling its spread. In this study, high-binding-affinity aptamers were identified using the GO-Cell-SELEX strategy, and two aptamers (52 and 91) were selected for further testing. Aptamer 91 was able to detect various strains of the virus in over 97% of clinical samples, while aptamer 52 showed potential for use in a future diagnostic kit. These findings suggest the potential for developing a new rapid aptamer-based coronavirus diagnostic kit.
Article
Biology
Samaneh Sarvary, Seyed Latif Mousavi Gargari, Shahram Nazarian, Razieh Rezaei Adriani, Nafiseh Noroozi
Summary: Shigellosis, an acute inflammatory disease caused by Shigella, is a significant cause of mortality in children in developing countries. Although no safe and effective vaccine is currently available, a new nanoparticle-based vaccine has shown promising protective effects in mouse models, suggesting a potential vaccination strategy for the future.
Article
Chemistry, Applied
Zahra Aghajani, Iraj Rasooli, Seyed Latif Mousavi Gargari
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2019)