Article
Hematology
Stephanie N. Hurwitz, Seul K. Jung, Danielle R. Kobulsky, Hossein Fazelinia, Lynn A. Spruce, Empar Baltasar Perez, Nathalie Groen, Clementina Mesaros, Peter Kurre
Summary: This study demonstrates that inhibition of nSMase-2 can significantly improve the transplantation efficiency of HSPC after ex vivo culture. The blockade of nSMase-2 activates ISR and promotes metabolic quiescence, partly due to disrupted sphingolipid metabolism that impairs the release of nSMase-2-dependent EVs. These findings reveal the importance of EV trafficking and ISR in maintaining HSPC homeostasis and long-term fitness, and suggest that transient nSMase-2 inhibition can enhance HSPC potency during ex vivo graft manipulation.
Review
Cell & Tissue Engineering
Yunzi Chen, Ye Li, Feng Lu, Ziqing Dong
Summary: In mammals, stem cell function plays a crucial role in post-injury repair and regenerative events. Stem cell transplantation has achieved success in animals but is not ideal for humans. However, evidence suggests that endogenous stem cells can be improved for tissue repair and regeneration. Artificially amplifying factors that promote stem cell mobilization or enhance the homing of endogenous stem cells can enhance tissue regeneration. Bone marrow-derived stem cells hold therapeutic potential for the regeneration of multiple injured tissues. Strategies involving the mobilization or homing of these stem cells have shown promise in animal models and human trials, offering a safer and more efficient approach for in situ tissue regeneration.
Article
Biology
Mateusz Nowicki, Janusz Szemraj, Agnieszka Wierzbowska, Agnieszka Pluta, Olga Grzybowska-Izydorczyk, Aleksandra Nowicka, Piotr Stelmach, Magdalena Czemerska, Anna Szmigielska-Kaplon
Summary: Lymphoproliferative disorders are a diverse group of hematological malignancies characterized by abnormal lymphocyte proliferation. Autologous hematopoietic stem cell transplantation is crucial in their treatment. miRNAs, especially hsa-miR-146a-5p, might play a significant role in influencing the efficacy of hematopoietic stem cell mobilization.
Review
Hematology
Jian Chen, Hillard M. Lazarus, Parastoo B. Dahi, Scott Avecilla, Sergio A. Giralt
Summary: Hematopoietic cell transplantation (HCT) is a primary treatment for many cancers, with mobilization being the first essential step. Poor mobilization is a major factor affecting successful transplantation. Novel agents are being investigated to enhance mobilization response, and further understanding of the molecular mechanism of mobilization may lead to new therapeutic strategies for successful mobilization.
Article
Cell & Tissue Engineering
Tso-Fu Wang, Yu-Shan Liou, Shang-Hsien Yang, Guan-Ling Lin, Ya-Wen Chiang, Te-Sheng Lien, Chi-Cheng Li, Jen-Hung Wang, Hsin-Hou Chang, Der-Shan Sun
Summary: P-selectin is identified as a critical factor for G-CSF-induced HSC mobilization. Blocking the interaction between P-selectin and PSGL-1 can facilitate the mobilization of HSCs from bone marrow to peripheral circulation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Oncology
Mateusz Nowicki, Agnieszka Wierzbowska, Emilia Stec-Martyna, Dominika Kulczycka-Wojdala, Grzegorz Nowicki, Anna Szmigielska-Kaplon
Summary: The expression levels of SIRT5, SIRT6, and SIRT7 may be associated with the efficacy of hematopoietic stem cell mobilization. Upregulated expressions of SIRT3 and SIRT7 were observed in the complete remission group. These findings contribute to a better understanding of the role of sirtuins during hematopoietic stem cell transplantation.
Article
Multidisciplinary Sciences
Xin Gao, Dachuan Zhang, Chunliang Xu, Huihui Li, Kathleen M. Caron, Paul S. Frenette
Summary: Nociceptive nerves play a role in enforcing HSC mobilization through CGRP secretion, collaborating with sympathetic nerves to maintain HSCs in the bone marrow. CGRP directly activates HSCs via the G alpha(s)/adenylyl cyclase/cAMP pathway, and consumption of capsaicin-containing food enhances HSC mobilization in mice.
Article
Medicine, General & Internal
Tso-Fu Wang, Yu-Shan Liou, Hsin-Hou Chang, Shang-Hsien Yang, Chi-Cheng Li, Jen-Hung Wang, Der-Shan Sun
Summary: This study investigated the correlation between body mass index (BMI), proinflammatory cytokine levels, and hematopoietic stem cell (HSC) mobilization triggered by granulocyte colony-stimulating factor (G-CSF). The results showed that BMI was positively correlated with G-CSF-induced HSC mobilization, and good mobilizers had higher BMI and proinflammatory cytokine levels compared to poor mobilizers.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Trieu Hai Nguyen, Belma Melda Abidin, Krista M. Heinonen
Summary: FZD6 plays a protective role in the bone marrow against inflammatory response and its deficiency leads to impaired mobilization of HSPCs and increased release of pro-inflammatory cytokines.
Article
Cell Biology
Zhencan Shi, Daojun Diao, Yanan Zhao, Ying Luo, Yafei Li, Dingdong Liu, Kai Zhang, Yugang Qiu, Li Yu, Zhangfa Song, Zhenyu Ju
Summary: The research reveals a new role of CHOP in regulating HSCs function and homeostasis by reducing ATF3 and ROS signaling, leading to improved HSCs homeostasis and function.
Article
Biochemistry & Molecular Biology
Irwin Puc, Tzu-Chuan Ho, Yu-Wen Chien, Sia-Seng Tan, Yu-Cin Fong, Yi-Ju Chen, Sheng-Hsuan Wang, Yun-Hsuan Li, Chun-Hong Chen, Po-Lin Chen, Guey-Chuen Perng, Jih-Jin Tsai
Summary: DENV infection can induce mobilization of hematopoietic stem and progenitor cells (HSPCs), which are also susceptible to DENV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Jiewu Huang, Yaozhong Kong, Chao Xie, Lili Zhou
Summary: Renal failure is a global public health problem, where stem/progenitor cell therapy shows potential. Understanding the characteristics and homing capacity of stem/progenitor cells in the kidney could greatly advance research and therapy in this field.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Chengxin Luo, Li Wang, Guixian Wu, Xiangtao Huang, Yali Zhang, Yanni Ma, Mingling Xie, Yanni Sun, Yarui Huang, Zhen Huang, Qiuyue Song, Hui Li, Yu Hou, Xi Li, Shuangnian Xu, Jieping Chen
Summary: Through network meta-analysis comparing the efficacy of different HSC mobilization regimens, it was found that regimens including G-CSF plus AMD3100, Me6, EP80031, and ML141 showed promising results in terms of collecting CFCs and LSK cells.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biotechnology & Applied Microbiology
Lingxi Meng, Yige Wei, Yaxian Liang, Qin Hu, Huixu Xie
Summary: The regeneration of periodontal tissue is a challenging issue in oral diseases, and tissue engineering has shown promise in addressing this problem. However, the source of exogenous seed cells remains a major obstacle. In recent years, the application of endogenous cell homing has gained attention and shown potential in clinical tissue repair, periodontal regeneration, and cell therapy prospects. Further research is needed to improve the efficiency of endogenous cell homing and expand its clinical applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Dusan M. Maric, Gordana Velikic, Dusica L. Maric, Gordana Supic, Danilo Vojvodic, Vedrana Petric, Dzihan Abazovic
Summary: Transplanted stem cells can be directed to migrate to targeted tissues through direct delivery to the cerebrospinal fluid. However, the efficiency and mechanisms of this method still need improvement, and there is a discrepancy between in vivo and in vitro results, as well as a gap between preclinical and clinical research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Marwah M. Albakri, Frank A. Veliz, Steven N. Fiering, Nicole F. Steinmetz, Scott F. Sieg
Article
Biochemistry & Molecular Biology
Stefania De Santis, Giulio Verna, Grazia Serino, Raffaele Armentano, Elisabetta Cavalcanti, Marina Liso, Manuela Dicarlo, Sergio Coletta, Mauro Mastronardi, Antonio Lippolis, Angela Tafaro, Angelo Santino, Aldo Pinto, Pietro Campiglia, Alex Y. Huang, Fabio Cominelli, Theresa T. Pizarro, Marcello Chieppa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
Soumya Panigrahi, Douglas A. Bazdar, Marwah Albakri, Brian Ferrari, Christopher J. Antonelli, Michael L. Freeman, George Dubyak, Chad Zender, Scott F. Sieg
EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Oncology
Lei Wang, Shuiliang Yu, Ernest R. Chan, Kai-Yuan Chen, Cui Liu, Danian Che, Amad Awadallah, Jay Myers, David Askew, Alex Y. Huang, Ivan Maillard, Dan Huang, Wei Xin, Lan Zhou
Summary: The study found that Notch2 signaling plays an important role in cDC1-mediated antitumor immunity by regulating DC terminal differentiation, CCR7 expression, and antigen presentation, leading to inhibition of inflammation-associated colon cancer development. Suppressed cDC1 gene signature expression in human colorectal cancer is associated with poor prognosis.
CANCER IMMUNOLOGY RESEARCH
(2021)
Meeting Abstract
Oncology
Katherine Daunov, Michael Daunov, Kara Noskoff, Hilary Gan, Simon Davies, Olivia Larbi, Megan Farrell, Whitney Hadley, Amelia Renee Baffa, Jennifer Giesel, Rachel A. Egler, Alex Yee-Chen Huang, John J. Letterio, Richard T. Lee
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
Sarah B. Whittle, Katharine Offer, Ryan D. Roberts, Amy LeBlanc, Cheryl London, Robbie G. Majzner, Alex Y. Huang, Peter Houghton, E. Alejandro Sweet Cordero, Patrick J. Grohar, Michael Isakoff, Michael W. Bishop, Elizabeth Stewart, Emily K. Slotkin, Emily Greengard, Scott C. Borinstein, Fariba Navid, Richard Gorlick, Katherine A. Janeway, Damon R. Reed, Pooja Hingorani
Summary: Osteosarcoma is the most common bone tumor in children and young adults with poor prognosis for metastatic and relapsed disease. The Children's Oncology Group Bone Tumor Committee established a task force to prioritize agents for clinical trials, identifying multitargeted tyrosine kinase inhibitors as the top agents of interest. Only these inhibitors met all criteria for frontline evaluation and have been incorporated into an upcoming phase III study concept. The task force will continue to reassess identified agents and evaluate novel agents using this method as new data become available.
PEDIATRIC BLOOD & CANCER
(2021)
Article
Multidisciplinary Sciences
Jeong-su Do, Daniel Zwick, Jonathan D. Kenyon, Fei Zhong, David Askew, Alex Y. Huang, Wouter Van't Hof, Marcie Finney, Mary J. Laughlin
Summary: Co-culturing human iTregs with MSCs enhances the stability of FOXP3 expression and suppressive function, with a key mechanism being MSC mt transfer via tunneling nanotubules. Mitochondrial metabolic function drives MSC mt transfer to proliferating iTregs, stabilizing FOXP3 expression and promoting suppressive function in inflammatory conditions.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Zainab Albar, Marwah Albakri, Jamal Hajjari, Mohamad Karnib, Scott E. Janus, Sadeer G. Al-Kindi
Summary: The study found that interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha), and C-reactive protein (CRP) are associated with the incidence of heart failure (HF), with IL-6 and CRP specifically linked to HF with preserved ejection fraction (HFpEF), but not with HF with reduced ejection fraction (HFrEF) or HF with midrange EF (HFmrEF).
AMERICAN JOURNAL OF CARDIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Byung-Gyu Kim, Sung Hee Choi, John J. Letterio, Jie-Young Song, Alex Y. Huang
Summary: The study demonstrates that overexpression of VEGF in plasmacytoma inhibits tumor growth and enhances T-cell-mediated antitumor immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Kristen A. A. VanHeyst, Sung Hee Choi, Daniel T. T. Kingsley, Alex Y. Y. Huang
Summary: Vascular Cell Adhesion Molecule-1 (VCAM-1) is a membrane protein with critical physiological functions in cellular immune response. It can be cleaved into a soluble form (sVCAM-1) and elevated concentrations of sVCAM-1 have been found in the peripheral blood of cancer patients. The VCAM-1/VLA-4 signaling plays a vital role in tumor metastasis and therapy resistance.
Article
Oncology
Veronique Roche, Victor Sandoval, Claire Wolford, Zachary Senders, Julian Anthony Kim, Susan Pereira Ribeiro, Alex Yicheng Huang, Rafick-Pierre Sekaly, Joshua Lyons, Mei Zhang
Summary: This study found that the carbohydrate ligand BG34-200 can bind to activated CD11b, resulting in significant effects on tumor-associated inflammatory monocytes (TAIMs) in three solid tumor backgrounds (osteosarcoma, advanced melanoma, and PDAC). This binding triggers structural biological changes in TAIMs, inducing F-actin cytoskeletal rearrangement, effective phagocytosis, and ICAM-1 clustering. These changes lead to the differentiation of TAIMs into monocyte-derived dendritic cells, enhancing T-cell activation in the tumor microenvironment and potentially improving immunotherapy for solid cancers.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Oncology
Mohammad Alshebremi, Suzanne L. Tomchuck, Jay T. Myers, Daniel T. Kingsley, Saada Eid, Muta Abiff, Melissa Bonner, Shahrazad T. Saab, Sung Hee Choi, Alex Yee-Chen Huang
Summary: The tumor cell-intrinsic STING pathway plays a crucial role in the effectiveness of cryoablation, and the expression of STING-related signaling components may serve as a potential therapy response biomarker.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Shuiliang Yu, Weihuan Wang, Marwah Albakri, Xiaoxing Yu, Gurnoor Majihail, Seunghwan Lim, Rachel K. Lopilato, Atsuko Ito, John Letterio, Robert S. Haltiwanger, Lan Zhou
Summary: The success of hematopoietic progenitor cell transplant therapy can be improved by mobilizing HPCs from the bone marrow niche in donors, with Notch receptor-ligand interactions playing a key role in retaining HPCs. Modifying O-fucose on Notch receptors can enhance the binding to ligands and effectively release HPCs from the niche, thus improving collection efficiency and therapy outcomes.
Review
Cell & Tissue Engineering
Marwah Albakri, Hammad Tashkandi, Lan Zhou
CELL TRANSPLANTATION
(2020)
Article
Oncology
Min Cui, Qiang Wang, Alison W. Xin, Danian Che, Zhengbin Lu, Lan Zhou, Wei Xin
Summary: The study reports pathological findings in the gastrointestinal tracts of COVID-19 patients, with three of six cases showing idiopathic inflammatory bowel disease. The enteric mucosa exhibited various histologic changes, including active enteritis, chronic active enteritis, and transmural necrosis. Extensive thrombi were found in vessels and/or the presence of vasculitis in three cases, indicating a poor prognosis for these patients.
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2021)