Article
Developmental Biology
Rudra Prasanna Banerjee, Martin Srayko
Summary: In the nematode Caenorhabditis elegans, two genetic suppressors, gskl-1 and gskl-2, were identified to interact with memi-1(sb41) and play a role in regulating sperm development and fertilization through modulating the major sperm protein (MSP).
Article
Genetics & Heredity
Ting Gong, Francis J. McNally
Summary: Errors in chromosome segregation during meiosis are a major cause of aneuploidy. Crossovers are crucial for accurate chromosome alignment and partitioning, as they hold homologous chromosomes together. However, Caenorhabditis elegans zim-2 mutants with crossover defects on chromosome V have fewer dead embryos than expected, especially in males. This discrepancy can be explained by the presence of redundant mechanisms to compensate for crossover loss, a higher production of euploid gametes, and the viability and fertility of trisomy of chromosome V. Additionally, live imaging showed that univalents can segregate independently of homology.
Article
Biochemistry & Molecular Biology
Shalini Trivedi, Jitka Blazickova, Nicola Silva
Summary: This study reveals the important role of the BRC-1/BRCA1-BRD-1/BARD1 and PARG-1/PARG complex in meiotic cells for accurate DNA repair and genome integrity. Depletion of both BRC-1 and PARG-1 leads to synthetic lethality and reduces crossover formation and break repair. These factors function independently in developing oocytes, operating in different pathways. The study also highlights the influence of BRC-1-PARG-1 in DNA repair pathway choice.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biology
Samuel J. P. Taylor, Laura Bel Borja, Flavie Soubigou, Jack Houston, Dhanya K. Cheerambathur, Federico Pelisch
Summary: Phosphorylation is an important post-translational modification used for regulating protein localization and activity. Polo-like kinase 1 (PLK-1) is crucial for mitotic and meiotic cell divisions, but its specific roles and regulation in meiosis have been unclear. In this study, using C. elegans oocytes, we demonstrate that PLK-1 is involved in spindle assembly, chromosome alignment and segregation, and polar body extrusion during meiosis I. We also uncover the mechanisms of PLK-1 recruitment to meiotic chromosomes. Our findings reveal the essential functions of PLK-1 in oocyte meiosis and provide insights into its targeting mechanisms.
Article
Cell Biology
Tereza Pauerova, Lenka Radonova, Adela Horakova, Jason G. G. Knott, Martin Anger
Summary: The study found that the expression of securin protein in oocytes during female meiosis is compartmentalized, accumulating on the spindle during meiosis I, possibly impacting the regulation of separase activity directly.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yuanyuan Liu, Qiuchen Zhao, Hui Nie, Fengguo Zhang, Tingting Fu, Zhenguo Zhang, Feifei Qi, Ruoxi Wang, Jun Zhou, Jinmin Gao
Summary: The SYP-5 protein is identified as a critical regulator of meiotic thermotolerance, affecting crossover designation and bivalent formation. Homolog segregation errors become more severe at elevated temperature, with defects during the pachytene stage responsible for reduced viability in syp-5 mutants. These findings reveal a novel molecular mechanism for regulating meiotic thermotolerance.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Biology
David E. Almanzar, Spencer G. Gordon, Chloe Bristow, Antonia Hamrick, Lexy von Diezmann, Hanwenheng Liu, Ofer Rog
Summary: During meiosis, double-strand DNA breaks are repaired to form exchanges between parental chromosomes. The synaptonemal complex plays a role in promoting exchanges between both parental chromosomes and sister chromatids, by recruiting proexchange factors to repair sites. This mechanism is independent of specific chromosome conformation.
LIFE SCIENCE ALLIANCE
(2023)
Article
Cell Biology
Tereza Pauerova, Lenka Radonova, Adela Horakova, Jason G. Knott, Martin Anger
Summary: Chromosome segregation during female meiosis is crucial for proper cell division, with the study focusing on the role of the protein inhibitor securin in regulating separase activity. The research revealed compartmentalized expression of securin in oocytes, suggesting a potential impact on separase regulation near the chromosomes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Khairun Nisaa, Anat Ben-Zvi
Summary: Muscle proteostasis is regulated by the myogenic transcription factor MyoD. This study demonstrates that MyoD can modulate chaperone expression in terminally differentiated muscle cells, indicating its role in muscle maintenance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Christian Roedelsperger, Annabel Ebbing, Devansh Raj Sharma, Misako Okumura, Ralf J. Sommer, Hendrik C. Korswagen
Summary: Comparing the spatially resolved transcriptomes of Caenorhabditis elegans and Pristionchus pacificus, researchers found that regional gene expression in adult worms is driven by recent duplication and emergence of novel genes, rather than strong conservation at the level of single genes. Novel genes are enriched in sperm-related regions, supporting the out of testis hypothesis. Numerous novel genes are also found to have known functions during meiosis, suggesting rapid evolution even in universal processes like meiosis. This study highlights the importance of novel genes in generating phenotypic diversity and characterizes gene origination in sperm-related regions.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Genetics & Heredity
Debabrata Das, Shalini Trivedi, Jitka Blazickova, Swathi Arur, Nicola Silva
Summary: In this study, a phosphorylation site Serine 285 in HTP-3 protein during meiotic prophase I was identified. The phosphorylation at this site is independent of canonical meiotic protein kinases and does not regulate HTP-3-dependent meiotic processes.
G3-GENES GENOMES GENETICS
(2022)
Article
Developmental Biology
Silvan Spiri, Simon Berger, Louisa Mereu, Andrew DeMello, Alex Hajnal
Summary: During the development of Caenorhabditis elegans vulva, EGFR signaling in the uterine anchor cell plays a crucial role in aligning the uterus with the vulva.
Article
Biology
Isabelle Schiffer, Birgit Gerisch, Kazuto Kawamura, Raymond Laboy, Jennifer Hewitt, Martin Sebastian Denzel, Marcelo A. Mori, Siva Vanapalli, Yidong Shen, Orsolya Symmons, Adam Antebi
Summary: This study reveals that miR-1 in Caenorhabditis elegans plays a role in muscle function by regulating vacuolar ATPase subunits and lysosomal biogenesis, impacting muscle function and health by coordinating lysosomal v-ATPase and biogenesis. Deletion of mir-1 can improve mid-life muscle motility, pharyngeal pumping, and organismal longevity.
Article
Biology
Thanh Thi Vuong-Brender, Sean Flynn, Yvonne Vallis, Mario de Bono
Summary: CAMTAs are ancient proteins expressed broadly in nervous systems, controlling neuronal CaM levels. Loss of CAMT-1, the sole C. elegans CAMTA, results in behavioral and neuronal Ca2+ signaling defects.
Article
Cell Biology
Shitian Li, Umar Al-Sheikh, Yili Chen, Lijun Kang
Summary: Numerous taste receptors and related molecules have been discovered in vertebrates and invertebrates. Otopetrin1 has recently been identified as a sour taste receptor in mammals. However, the involvement of other Otopetrin proteins in pH-sensing is unknown. In this study, Otopetrin channels were found to be widely expressed in various tissues, especially in sensory neurons in nematodes. Mutations in otopetrin genes did not affect calcium responses or acid avoidance behaviors, indicating diverse functions of Otopetrin channels among species. This research reveals the evolutionarily conserved acid-sensitive proton channels in nematodes and provides insights for understanding Otopetrin channel function and mechanism.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Marina E. Crowder, Jonathan R. Flynn, Karen P. McNally, Daniel B. Cortes, Kari L. Price, Paul A. Kuehnert, Michelle T. Panzica, Armann Andaya, Julie A. Leary, Francis J. McNally
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Cell Biology
Karen Perry McNally, Michelle T. Panzica, Taekyung Kim, Daniel B. Cortes, Francis J. McNally
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Genetics & Heredity
Elizabeth Vargas, Karen McNally, Jacob A. Friedman, Daniel B. Cortes, David Y. Wang, Ian F. Korf, Francis J. McNally
Article
Cell Biology
Michelle T. Panzica, Harold C. Marin, Anne-Cecile Reymann, Francis J. McNally
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Jonathan R. Flynn, Francis J. McNally
MOLECULAR BIOLOGY OF THE CELL
(2017)
Editorial Material
Multidisciplinary Sciences
Francis J. McNally
Review
Cell Biology
Michelle T. Panzica, Francis J. McNally
Article
Biochemistry & Molecular Biology
Stanley Nithianantham, Francis J. McNally, Jawdat Al-Bassam
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Cell Biology
Francis J. McNally, Antonina Roll-Mecak
JOURNAL OF CELL BIOLOGY
(2018)
Article
Developmental Biology
Elizabeth Vargas, Karen P. McNally, Daniel B. Cortes, Michelle T. Panzica, Brennan M. Danlasky, Qianyan Li, Amy Shaub Maddox, Francis J. McNally
Article
Multidisciplinary Sciences
Franco L. Lombino, Mary Muhia, Jeffrey Lopez-Rojas, Monika S. Brill, Edda Thies, Laura Ruschkies, David Lutz, Melanie Richter, Torben J. Hausrat, Andre T. Lopes, Francis J. McNally, Irm Hermans-Borgmeyer, Jessica E. M. Dunleavy, Sabine Hoffmeister-Ullerich, Michael Frotscher, Thomas Misgeld, Michael R. Kreutz, Froylan Calderon de Anda, Matthias Kneussel
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Brennan M. Danlasky, Michelle T. Panzica, Karen P. McNally, Elizabeth Vargas, Cynthia Bailey, Wenzhe Li, Ting Gong, Elizabeth S. Fishman, Xueer Jiang, Francis J. McNally
JOURNAL OF CELL BIOLOGY
(2020)
Article
Genetics & Heredity
Karen P. McNally, Elizabeth A. Beath, Brennan M. Danlasky, Consuelo Barroso, Ting Gong, Wenzhe Li, Enrique Martinez-Perez, Francis J. McNally
Summary: Accurate chromosome segregation during meiosis requires both cohesion between chromosomes and the formation of a bipolar spindle. In this study, the researchers investigated whether the bipolar structure of C. elegans meiotic chromosomes is necessary for spindle polarity in the absence of cohesion. They found that while a mutant lacking cohesion formed an apolar spindle, a mutant with residual non-cohesive cohesin formed a bipolar spindle. The results suggest that cohesin is essential for spindle assembly and chromosome segregation independent of its role in sister chromatid cohesion.
Article
Genetics & Heredity
Ting Gong, Francis J. McNally
Summary: Errors in chromosome segregation during meiosis are a major cause of aneuploidy. Crossovers are crucial for accurate chromosome alignment and partitioning, as they hold homologous chromosomes together. However, Caenorhabditis elegans zim-2 mutants with crossover defects on chromosome V have fewer dead embryos than expected, especially in males. This discrepancy can be explained by the presence of redundant mechanisms to compensate for crossover loss, a higher production of euploid gametes, and the viability and fertility of trisomy of chromosome V. Additionally, live imaging showed that univalents can segregate independently of homology.